FastQCFastQC Report
Wed 25 May 2016
SRR1033029_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033029_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5605662
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93400.033.1182941
GGTATCA65450.032.5371
TCAACGC103450.028.4911824
GTACATG230600.027.7248061
CAACGCA106600.027.6059175
ATCAACG110800.026.9406073
AACGCAG110000.026.704426
TACATGG228850.026.580112
ACGCGTT950.001305387424.7362867
TATCAAC123600.024.3408052
ACATGGG244950.024.3149053
ACGCAGA133500.021.5807887
CATGGGG180550.021.0632024
GAGTACA160200.020.423761
CGCAGAG141300.020.074988
AGTACAT164700.018.8946042
GTATTAG27750.018.6345371
CATGGGA59000.018.165874
GTCTATA5600.017.6286951
GCAGAGT154350.017.5265149
Produced by FastQC (version 0.11.5)