FastQCFastQC Report
Wed 25 May 2016
SRR1033029_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033029_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5605662
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75140.1340430443362443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64600.11524062635242724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92750.044.2722851
GTATCAA151400.039.826521
TCAACGC179650.032.988424
CAACGCA180150.032.9229475
ATCAACG184750.032.2066963
AACGCAG186850.031.868176
TATCAAC202000.029.65022
GTACATG216150.027.15451
ACGCAGA220850.026.7279627
TACATGG214750.026.4415762
CGCAGAG228950.025.4541458
ACATGGG233050.023.837753
GCAGAGT242000.023.7513669
CATGGGG169350.020.5639554
GTCTATA5350.020.2675721
CAGAGTA236950.019.77515210-11
AGAGTAC221800.019.7379810-11
CATGGGA54650.018.9193484
GAGTACT129050.018.8099612-13
GTACTTT129950.018.46269214-15
Produced by FastQC (version 0.11.5)