FastQCFastQC Report
Wed 25 May 2016
SRR1033028_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033028_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3256464
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37400.033.5668871
TCAACGC37750.031.6286984
GGTATCA25750.031.2241231
ATCAACG38450.030.9306263
CAACGCA39900.029.9253125
AACGCAG41400.028.8353026
GTACATG85300.025.9071851
TATCAAC47600.025.6762122
TACATGG86400.024.9725862
ACGCAGA48750.024.102937
CGCAGAG49650.023.4072138
ACATGGG90700.022.4929813
TACACCG3850.020.7570725
CGCGTAT1301.6545528E-819.91767512-13
CATGGGG62050.019.0150074
GAGTACA62700.017.8476411
GCAGAGT61750.017.603299
GTATTAG14900.017.3559271
GTCTTAC12650.017.0977381
GTCCTAG13350.016.905631
Produced by FastQC (version 0.11.5)