FastQCFastQC Report
Wed 25 May 2016
SRR1033028_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033028_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3256464
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57700.17718605211051006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47960.14727630951854526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46350.049.518671
GTATCAA87200.045.4536971
TCAACGC101200.038.6846664
ATCAACG101700.038.496843
CAACGCA102550.038.129585
AACGCAG106000.036.9772456
TATCAAC109800.035.9548572
ACGCAGA123200.031.4715127
CGCAGAG125150.030.8309358
GCAGAGT140450.027.037349
GTACATG82550.026.7759861
TACATGG85850.024.693812
ACATGGG88150.023.3535463
GTGGTAT23000.022.9498521
GAGTACT94950.022.74372512-13
CAGAGTA138150.021.41505610-11
AGAGTAC129950.021.30165710-11
GGTCGAC2109.543801E-720.1417687
AGTACTT102050.020.01003512-13
GTACTTT107150.020.00078414-15
Produced by FastQC (version 0.11.5)