##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033028_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3256464
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71584577627758	34.0	31.0	34.0	31.0	34.0
2	32.717340956325636	34.0	31.0	34.0	31.0	34.0
3	32.81403510064905	34.0	31.0	34.0	31.0	34.0
4	36.30500198988842	37.0	37.0	37.0	35.0	37.0
5	36.28989142824855	37.0	37.0	37.0	35.0	37.0
6	36.35636874843389	37.0	37.0	37.0	35.0	37.0
7	36.30569415169337	37.0	37.0	37.0	35.0	37.0
8	36.31525882061033	37.0	37.0	37.0	35.0	37.0
9	38.120316699340144	39.0	39.0	39.0	37.0	39.0
10-11	38.08238107345882	39.0	39.0	39.0	36.0	39.0
12-13	38.05574328474075	39.0	39.0	39.0	35.0	39.0
14-15	39.55418039321178	41.0	40.0	41.0	37.0	41.0
16-17	39.55331288784399	41.0	40.0	41.0	37.0	41.0
18-19	39.574409850684674	41.0	40.0	41.0	37.0	41.0
20-21	39.56779577480359	41.0	40.0	41.0	37.0	41.0
22-23	39.49537611961932	41.0	39.0	41.0	36.5	41.0
24-25	39.44038794840047	41.0	39.0	41.0	36.0	41.0
26-27	39.32832145541913	41.0	39.0	41.0	36.0	41.0
28-29	39.27580421586113	41.0	39.0	41.0	36.0	41.0
30-31	39.27001066187128	41.0	39.0	41.0	36.0	41.0
32-33	39.22563369347857	41.0	39.0	41.0	35.5	41.0
34-35	39.143864940622706	41.0	39.0	41.0	35.0	41.0
36-37	39.05561630652143	41.0	39.0	41.0	35.0	41.0
38-39	38.96135824010338	41.0	39.0	41.0	35.0	41.0
40-41	38.86037892634465	41.0	39.0	41.0	35.0	41.0
42-43	38.741519175400065	41.0	39.0	41.0	35.0	41.0
44-45	38.57208079069813	41.0	38.5	41.0	35.0	41.0
46-47	38.372919829606595	41.0	38.0	41.0	34.5	41.0
48-49	38.16781085250751	40.0	38.0	41.0	34.0	41.0
50-51	38.02983911383636	40.0	38.0	41.0	34.0	41.0
52-53	37.89725512089186	40.0	38.0	41.0	34.0	41.0
54-55	37.75407543273931	40.0	37.5	41.0	33.0	41.0
56-57	37.609053408850826	40.0	37.0	41.0	33.0	41.0
58-59	37.43511950385449	40.0	37.0	41.0	33.0	41.0
60-61	37.2492619295039	40.0	36.0	41.0	33.0	41.0
62-63	37.0229564951432	39.5	36.0	41.0	32.5	41.0
64-65	36.76252109650221	39.0	35.0	41.0	32.0	41.0
66-67	36.49047340919476	39.0	35.0	41.0	32.0	41.0
68-69	36.19096695065568	38.0	35.0	40.5	31.5	41.0
70-71	35.86864156950607	37.0	35.0	40.0	31.0	41.0
72-73	35.52755657670406	37.0	35.0	39.5	31.0	41.0
74-75	35.18229420008942	36.5	35.0	39.0	31.0	41.0
76-77	34.82420702332346	36.0	35.0	39.0	31.0	40.5
78-79	34.4565792835419	35.5	35.0	37.5	30.0	39.5
80-81	34.11917420244781	35.0	34.0	37.0	30.0	39.0
82-83	33.8118548216716	35.0	34.0	37.0	30.0	39.0
84-85	33.57880879383282	35.0	34.0	36.0	30.0	38.0
86-87	33.359260381812916	35.0	34.0	36.0	30.0	37.0
88-89	33.14002964565246	35.0	34.0	36.0	30.0	37.0
90-91	32.95004950154524	35.0	34.0	35.0	29.5	36.5
92-93	32.7706180691695	35.0	34.0	35.0	29.0	36.0
94-95	32.61279657935724	35.0	34.0	35.0	29.0	36.0
96-97	32.463811361034544	35.0	34.0	35.0	29.0	36.0
98-99	32.337173080986005	35.0	34.0	35.0	29.0	36.0
100	32.22958491173248	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.13870942860881996
1101	2	0.08542163615408072
1101	3	0.14174840087638074
1101	4	0.11548029106918989
1101	5	0.08756412010757231
1101	6	0.10647948790054329
1101	7	0.1398185334114075
1101	8	0.12616279891566506
1101	9	0.14875446302307438
1101	10-11	0.2086218508732145
1101	12-13	0.21469779148605284
1101	14-15	0.27083477082677376
1101	16-17	0.25847032254341684
1101	18-19	0.24337977226009855
1101	20-21	0.27070459955395165
1101	22-23	0.27507804697554405
1101	24-25	0.35749701338829
1101	26-27	0.315661575821359
1101	28-29	0.25588712761582144
1101	30-31	0.2912377531598267
1101	32-33	0.2949209528396608
1101	34-35	0.2883499065750996
1101	36-37	0.2196083777724951
1101	38-39	0.24274816062439442
1101	40-41	0.2899074040589156
1101	42-43	0.3039903111298443
1101	44-45	0.2948061208375563
1101	46-47	0.2863007477387285
1101	48-49	0.3095548495840106
1101	50-51	0.36400952723359126
1101	52-53	0.3492334109661783
1101	54-55	0.36984014269926035
1101	56-57	0.31511026962076727
1101	58-59	0.275365246961627
1101	60-61	0.3093868057783098
1101	62-63	0.3151915667925351
1101	64-65	0.36142007097895146
1101	66-67	0.37362694781057826
1101	68-69	0.31505470241948785
1101	70-71	0.31551431330169066
1101	72-73	0.2789340212677871
1101	74-75	0.25921552784114965
1101	76-77	0.29151904349014757
1101	78-79	0.27328053319232026
1101	80-81	0.2594213966526908
1101	82-83	0.2722490457903177
1101	84-85	0.2870788463305658
1101	86-87	0.2976456909990688
1101	88-89	0.269206274135648
1101	90-91	0.2921539839775491
1101	92-93	0.3156228386634865
1101	94-95	0.3197314999200245
1101	96-97	0.28324395299556926
1101	98-99	0.2887649256026208
1101	100	0.2391588970879468
1102	1	0.16113845945641003
1102	2	0.17627032843935808
1102	3	0.19233712055268626
1102	4	0.1456212593075179
1102	5	0.1580001656426262
1102	6	0.17218353611167458
1102	7	0.1801949660824107
1102	8	0.16325038068445252
1102	9	0.26915718775101993
1102	10-11	0.2311997901811722
1102	12-13	0.2239606373147538
1102	14-15	0.3549657852715171
1102	16-17	0.30882638251618744
1102	18-19	0.26739002449561156
1102	20-21	0.25484298389976345
1102	22-23	0.2873060409848378
1102	24-25	0.3353973884719821
1102	26-27	0.29785334273039865
1102	28-29	0.3620185467540722
1102	30-31	0.344655082531645
1102	32-33	0.3491338669375992
1102	34-35	0.3691763407174804
1102	36-37	0.3982842591592757
1102	38-39	0.4251947229822193
1102	40-41	0.36446278157364276
1102	42-43	0.32191971789448104
1102	44-45	0.38117307993044136
1102	46-47	0.40407213132437647
1102	48-49	0.3464510483036207
1102	50-51	0.33264075431170426
1102	52-53	0.29355539699565725
1102	54-55	0.2603774761961404
1102	56-57	0.3108401020688021
1102	58-59	0.2945037341735457
1102	60-61	0.2648013783383689
1102	62-63	0.25690231614480297
1102	64-65	0.22135812214273187
1102	66-67	0.27085193266061935
1102	68-69	0.2833431900350547
1102	70-71	0.2946673371877253
1102	72-73	0.3180247577709068
1102	74-75	0.2978278718264633
1102	76-77	0.2772800300464624
1102	78-79	0.3345232391387327
1102	80-81	0.26023586186740744
1102	82-83	0.22850641731900367
1102	84-85	0.24119694131270109
1102	86-87	0.22944889745291164
1102	88-89	0.2518489591963373
1102	90-91	0.23215366626449452
1102	92-93	0.29721272163831003
1102	94-95	0.3592613835667322
1102	96-97	0.3333851920712547
1102	98-99	0.3748975322585011
1102	100	0.3888363792797378
1103	1	0.14022259500927703
1103	2	0.09036441904056147
1103	3	0.12087629418104484
1103	4	0.125213410213739
1103	5	0.14481742379653184
1103	6	0.10246227746515046
1103	7	0.16486727052215855
1103	8	0.14479248951263202
1103	9	0.19376052525795018
1103	10-11	0.1895835829519612
1103	12-13	0.1922943469557552
1103	14-15	0.25717282975108446
1103	16-17	0.2838438295081147
1103	18-19	0.3027689321086626
1103	20-21	0.2948145555658428
1103	22-23	0.2763246990032613
1103	24-25	0.25695263385878775
1103	26-27	0.2438908229742438
1103	28-29	0.2794173028861806
1103	30-31	0.29662454003282335
1103	32-33	0.2779963230635474
1103	34-35	0.24384279528345587
1103	36-37	0.2637320310757758
1103	38-39	0.31369048438946834
1103	40-41	0.33509795470937576
1103	42-43	0.23793554334245215
1103	44-45	0.25110575506744937
1103	46-47	0.26799842291492126
1103	48-49	0.24453239233777424
1103	50-51	0.24846671543569698
1103	52-53	0.29293448934156174
1103	54-55	0.2929340762905568
1103	56-57	0.2064532098912153
1103	58-59	0.23958676132927792
1103	60-61	0.2043116310118691
1103	62-63	0.18719132043470665
1103	64-65	0.24988138821962025
1103	66-67	0.26322400515799416
1103	68-69	0.28656062060629495
1103	70-71	0.28031718632256286
1103	72-73	0.2572681740971845
1103	74-75	0.26149194539119236
1103	76-77	0.2283283890029253
1103	78-79	0.20813517698639572
1103	80-81	0.20582177290827275
1103	82-83	0.14373969386409868
1103	84-85	0.13660735614059405
1103	86-87	0.12107485447173616
1103	88-89	0.15135171992623242
1103	90-91	0.09966685413780851
1103	92-93	0.10940355067831575
1103	94-95	0.13857489574883175
1103	96-97	0.19348597410700563
1103	98-99	0.21696890071844166
1103	100	0.2110104797333605
1104	1	0.06495475575877663
1104	2	0.04919866692369368
1104	3	0.07684510205542239
1104	4	0.053115225337201366
1104	5	0.08006423783984928
1104	6	0.11434146231937348
1104	7	0.08731696551651424
1104	8	0.0938289565304018
1104	9	0.1147034486941223
1104	10-11	0.16202229534059143
1104	12-13	0.1947435538343001
1104	14-15	0.23338626468114398
1104	16-17	0.2053269455631792
1104	18-19	0.21615633284811508
1104	20-21	0.20933887526872752
1104	22-23	0.20917159442464595
1104	24-25	0.22204315360107785
1104	26-27	0.19897453960242473
1104	28-29	0.21538961718623995
1104	30-31	0.16804606483415796
1104	32-33	0.14505412112355032
1104	34-35	0.12792327471233733
1104	36-37	0.0934277099352272
1104	38-39	0.08091322650587784
1104	40-41	0.1192384380622471
1104	42-43	0.08921883508107697
1104	44-45	0.14602085628379058
1104	46-47	0.06608219178674801
1104	48-49	-0.027941646479249016
1104	50-51	0.04518671542806629
1104	52-53	0.05574419262625696
1104	54-55	0.05436787930188558
1104	56-57	0.10875556731897262
1104	58-59	0.035116955200862776
1104	60-61	0.0380703496468513
1104	62-63	-0.03290748678948319
1104	64-65	-0.04520537817901982
1104	66-67	0.029558748307067617
1104	68-69	-0.01053693029169267
1104	70-71	0.033064196279553926
1104	72-73	0.0013272231446208593
1104	74-75	-0.017641895010577002
1104	76-77	0.023456483175714027
1104	78-79	-0.02931981768723091
1104	80-81	0.0234059352281335
1104	82-83	-0.01678772480217816
1104	84-85	0.01438077392559478
1104	86-87	0.08360236103698782
1104	88-89	0.03561781683209375
1104	90-91	0.08608729593220232
1104	92-93	0.049375149392965056
1104	94-95	0.07911588346262022
1104	96-97	0.09335001669358434
1104	98-99	0.09901645406654325
1104	100	0.11837305844918689
1105	1	0.07085533817874534
1105	2	0.10028559273968085
1105	3	0.08773182175213634
1105	4	0.060785776784420875
1105	5	0.060288224561034554
1105	6	0.09441688884484023
1105	7	0.10266652491439743
1105	8	0.12769126472432646
1105	9	0.11866310358467302
1105	10-11	0.1445851909278133
1105	12-13	0.1874646299184164
1105	14-15	0.21057612653113722
1105	16-17	0.1704478685343105
1105	18-19	0.18388204172480016
1105	20-21	0.1817519554408591
1105	22-23	0.1803231242701102
1105	24-25	0.22760604726845202
1105	26-27	0.23969383790096543
1105	28-29	0.25301689784808445
1105	30-31	0.23920187321326836
1105	32-33	0.25339223809518785
1105	34-35	0.2644346443212342
1105	36-37	0.2770250050243277
1105	38-39	0.28194019799795456
1105	40-41	0.25280269336381167
1105	42-43	0.27616675682450165
1105	44-45	0.2792741865234163
1105	46-47	0.2795641301124121
1105	48-49	0.24621147584009861
1105	50-51	0.22297660962303922
1105	52-53	0.21487529192508248
1105	54-55	0.20308095489379951
1105	56-57	0.13789951227509079
1105	58-59	0.19875276532064134
1105	60-61	0.17732014077122216
1105	62-63	0.2515851508871947
1105	64-65	0.2556128136706093
1105	66-67	0.2357646424991202
1105	68-69	0.3036936874252518
1105	70-71	0.31923631839657673
1105	72-73	0.2865738609531334
1105	74-75	0.27519886573831087
1105	76-77	0.20860682655089846
1105	78-79	0.30729761018816504
1105	80-81	0.2369673875416396
1105	82-83	0.19311277003583172
1105	84-85	0.15447157574565296
1105	86-87	0.18330076555533736
1105	88-89	0.23324666811803496
1105	90-91	0.2551430391002327
1105	92-93	0.26910669517689456
1105	94-95	0.21644803672517554
1105	96-97	0.28625815063399784
1105	98-99	0.29314246163608004
1105	100	0.2838760645304603
1106	1	0.02946292143743534
1106	2	0.0016170364495309286
1106	3	0.0535063920701333
1106	4	0.022719392081917533
1106	5	0.028698302444233548
1106	6	0.050072008213668084
1106	7	0.07545519542065193
1106	8	0.09985650625238662
1106	9	0.07489517432289716
1106	10-11	0.06998758043603459
1106	12-13	0.07203346972197977
1106	14-15	0.0990631073841115
1106	16-17	0.0981419885576642
1106	18-19	0.1379033246256256
1106	20-21	0.1342969627147852
1106	22-23	0.145482128363291
1106	24-25	0.10677439860283044
1106	26-27	0.16889262682727235
1106	28-29	0.13010865233587765
1106	30-31	0.1337528278738347
1106	32-33	0.1404459700549765
1106	34-35	0.14793812472532863
1106	36-37	0.15352517468507187
1106	38-39	0.1423506843948701
1106	40-41	0.15759134604781622
1106	42-43	0.12925968443510527
1106	44-45	0.10086429385174966
1106	46-47	0.11781025735692907
1106	48-49	0.1765099145069513
1106	50-51	0.17067309371424955
1106	52-53	0.24681598020604412
1106	54-55	0.24345794071952298
1106	56-57	0.18982806221213622
1106	58-59	0.1734637042976388
1106	60-61	0.22543748310466327
1106	62-63	0.22270818445625906
1106	64-65	0.19225994518092193
1106	66-67	0.17658567295313787
1106	68-69	0.1956764921078218
1106	70-71	0.19037461444357007
1106	72-73	0.16484603313135437
1106	74-75	0.17784081202486846
1106	76-77	0.20042833995270115
1106	78-79	0.14478568064402708
1106	80-81	0.19228986876101573
1106	82-83	0.19004013706531708
1106	84-85	0.18328323764166754
1106	86-87	0.1156082687537392
1106	88-89	0.1923835457243186
1106	90-91	0.18553299147990288
1106	92-93	0.18214547287868044
1106	94-95	0.17901591412773143
1106	96-97	0.18452993491639091
1106	98-99	0.15759034084182133
1106	100	0.10362838430479826
1107	1	0.021685197827572722
1107	2	0.009582084567881566
1107	3	0.02728688781559896
1107	4	0.02181131899664024
1107	5	0.07255160431417096
1107	6	0.06685574991553977
1107	7	0.06448026651882088
1107	8	0.04735079886528837
1107	9	0.02991375150476472
1107	10-11	0.07709839874362245
1107	12-13	0.05672436591782315
1107	14-15	0.11965315120335163
1107	16-17	0.09756766029633468
1107	18-19	0.08693398707653444
1107	20-21	0.08289988417573113
1107	22-23	0.12182806831326332
1107	24-25	0.1303508241640401
1107	26-27	0.13254950175360136
1107	28-29	0.08733968723199581
1107	30-31	0.07956261903538575
1107	32-33	0.029748788971609486
1107	34-35	0.12571784011896625
1107	36-37	0.13889883699273753
1107	38-39	0.13060963665316194
1107	40-41	0.14066306228424708
1107	42-43	0.18943526192717997
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2216	50-51	-0.3577400904683685
2216	52-53	-0.3371346500837902
2216	54-55	-0.270949841827715
2216	56-57	-0.2832939260931937
2216	58-59	-0.29640185854131573
2216	60-61	-0.29877722413545627
2216	62-63	-0.29930870656047404
2216	64-65	-0.439254256363256
2216	66-67	-0.42436540736043327
2216	68-69	-0.4418757866843137
2216	70-71	-0.36404969148396304
2216	72-73	-0.4176110910333293
2216	74-75	-0.4809639117116191
2216	76-77	-0.4099455173962596
2216	78-79	-0.46717305169369183
2216	80-81	-0.4283673076993395
2216	82-83	-0.41948482723127256
2216	84-85	-0.46574653712766434
2216	86-87	-0.5218765807624948
2216	88-89	-0.606019227945346
2216	90-91	-0.5485794623190543
2216	92-93	-0.48736129327942734
2216	94-95	-0.42295531676820985
2216	96-97	-0.5685177873179441
2216	98-99	-0.5544328291674745
2216	100	-0.58780525461788
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12.0
3	3.0
4	11.0
5	33.0
6	75.0
7	196.0
8	409.0
9	770.0
10	1423.0
11	2313.0
12	3672.0
13	5572.0
14	6746.0
15	8874.0
16	10718.0
17	15108.0
18	18070.0
19	9661.0
20	6393.0
21	5917.0
22	6331.0
23	6733.0
24	7661.0
25	9444.0
26	11784.0
27	15396.0
28	19824.0
29	25725.0
30	33165.0
31	42062.0
32	54019.0
33	70414.0
34	97617.0
35	148000.0
36	266590.0
37	604504.0
38	1147527.0
39	591335.0
40	2357.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.48387317775434	20.168965656020728	13.75940443277549	23.587756733449442
2	15.373081677682448	20.628706129766254	40.34248455984476	23.655727632706533
3	17.857399496985906	25.974965989737036	30.500219569643498	25.667414943633553
4	12.07795797160642	17.1956810804669	39.4660311942439	31.260329753682782
5	12.2930071461188	38.04157468263783	36.209305032193	13.456113139050371
6	30.16996057990561	38.90379771168762	17.792751087760607	13.133490620646155
7	26.399032815962343	33.0417594052936	23.07355462857873	17.485653150165334
8	24.780796505641575	36.28169825475541	20.6758036130767	18.261701626526317
9	24.95181890541397	16.41283306064492	21.582213099853092	37.053134934088014
10-11	23.488635613375937	26.865047620948612	29.354910032259106	20.29140673341635
12-13	24.65573087864629	24.222561649691198	29.550841034938507	21.570866436724003
14-15	21.689670187670163	26.071240593307206	27.06707717403387	25.172012044988755
16-17	20.80139746777292	29.588465885440378	27.498298383076232	22.111838263710474
18-19	20.504297779575236	28.122058721017893	30.397128897151465	20.976514602255403
20-21	21.739936857873097	27.453098653354886	30.01782916004266	20.789135328729362
22-23	21.306075296215198	27.24940299408084	30.087946767938238	21.356574941765725
24-25	21.189220578259548	27.435207360705462	30.03687784188007	21.33869421915492
26-27	20.98495958109093	27.33114823972116	30.156855983271395	21.527036195916516
28-29	21.042854243123738	27.371741742636246	30.21117736239109	21.374226651848925
30-31	21.330447786860578	27.867561708112493	29.67811672798069	21.12387377704624
32-33	20.964073301593388	27.51780765885943	30.215780060826713	21.302338978720478
34-35	21.09229520117526	27.340176338507042	30.190614728122284	21.376913732195412
36-37	21.427459354686558	27.667540620746923	29.5978859278039	21.30711409676262
38-39	21.375240137769065	27.83032454834446	29.614760058763125	21.17967525512335
40-41	21.21038108229115	27.654092079072317	29.76738251513452	21.36814432350201
42-43	21.199082808838053	27.534543603122895	29.604902741132715	21.66147084690634
44-45	21.418569343926418	27.696667305396282	29.419609736204666	21.465153614472634
46-47	21.471479494322676	27.950224538026525	29.106094831694744	21.472201135956055
48-49	21.500114848498246	27.92063723105798	28.86710861842784	21.712139302015927
50-51	21.60697922654757	27.910948808277936	28.7020062251571	21.780065740017392
52-53	21.6029257501388	27.92211122247935	28.659874637029613	21.815088390352237
54-55	21.633050449813048	28.253360086277628	28.467518756540837	21.646070707368484
56-57	21.44655982685514	27.945587606680128	28.65075431510989	21.957098251354846
58-59	21.317831242722168	27.6387824339529	28.99603680556579	22.04734951775914
60-61	21.57418291742209	28.029129141301727	28.61815760898938	21.7785303322868
62-63	21.385204933940617	27.988471544595612	28.63486284509824	21.99146067636553
64-65	21.418093367529934	27.697941693812677	28.812187083904504	22.071777854752884
66-67	21.347172884453812	27.79057284219939	28.651076750733314	22.211177522613486
68-69	21.77802105508824	27.86512644491752	28.568851274736428	21.788001225257815
70-71	21.71954330211286	27.747508915730208	28.5670022096056	21.965945572551327
72-73	21.525462504471886	27.917720343133865	28.59284098812973	21.963976164264515
74-75	21.597050906682828	27.977417799121344	28.42581903009051	21.999712264105316
76-77	21.67317065381346	27.960158626043462	28.287154410428002	22.07951630971508
78-79	21.767367918085384	28.05380314353237	28.37537893862791	21.803449999754335
80-81	21.694205739722594	27.846953014066788	28.4870798510286	21.971761395182014
82-83	21.493880086640107	27.960780719060068	28.575589532128376	21.96974966217145
84-85	21.48502179050651	27.88561458072314	28.42971701821362	22.199646610556727
86-87	21.5572193295034	28.02398131463323	28.309537496637443	22.10926185922593
88-89	21.35679178969382	27.89190193450119	28.41336815711853	22.337938118686463
90-91	21.302046802661593	27.798861072179182	28.473441190813404	22.42565093434582
92-93	21.388013402957302	27.963296176766967	28.32881741764141	22.319873002634314
94-95	21.181784207276085	27.758748870318563	28.4577311052622	22.60173581714315
96-97	21.113167197714326	27.811538064693643	28.456211292276016	22.61908344531601
98-99	21.135696431093887	27.86036063701074	28.333420339878273	22.670522592017097
100	21.244147025730978	27.793520825042133	28.214099710606348	22.74823243862054
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24.0
1	26.5
2	119.0
3	214.5
4	145.0
5	102.5
6	279.5
7	652.5
8	1153.5
9	1388.0
10	1126.5
11	888.0
12	968.5
13	1144.0
14	1368.5
15	1627.5
16	1912.5
17	2310.0
18	2710.0
19	3121.0
20	3775.5
21	4855.0
22	6393.0
23	8568.0
24	11573.0
25	15574.0
26	20800.5
27	27483.0
28	35730.0
29	45300.5
30	55410.0
31	65519.0
32	75484.5
33	85460.0
34	95022.5
35	103034.0
36	109369.0
37	114716.5
38	119068.5
39	121078.0
40	121244.5
41	121026.5
42	119913.5
43	118151.5
44	115564.5
45	112879.5
46	110607.5
47	106940.0
48	103800.0
49	100707.5
50	97279.5
51	93946.0
52	87767.0
53	82611.0
54	79174.0
55	74742.0
56	68798.0
57	62439.5
58	56473.5
59	50395.5
60	44102.5
61	37218.5
62	31136.0
63	25847.5
64	20940.0
65	16524.5
66	12721.5
67	9614.0
68	7536.0
69	5940.0
70	4361.5
71	3176.0
72	2394.0
73	1722.0
74	1203.5
75	795.0
76	462.5
77	294.0
78	209.0
79	134.0
80	85.5
81	40.5
82	21.0
83	11.5
84	4.0
85	5.0
86	5.5
87	3.5
88	4.5
89	4.0
90	1.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04554019328940839
2	0.23927794073571826
3	0.002886566533516108
4	0.002886566533516108
5	3.3778970073060837E-4
6	9.212446383562048E-5
7	0.0
8	4.6062231917810235E-4
9	0.0
10-11	5.834549376255964E-4
12-13	0.0
14-15	1.8424892767124096E-4
16-17	7.677038652968373E-5
18-19	4.6062231917810235E-4
20-21	3.070815461187349E-5
22-23	1.6889485036530419E-4
24-25	0.0011362017206393191
26-27	1.6889485036530419E-4
28-29	2.1495708228311443E-4
30-31	1.2283261844749397E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	6.141630922374699E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	1.996030049771777E-4
70-71	1.5354077305936747E-5
72-73	2.763733915068614E-4
74-75	1.6889485036530419E-4
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.381866957534307E-4
84-85	0.0
86-87	3.684978553424819E-4
88-89	0.0021188626682192707
90-91	0.0020881545136073975
92-93	5.681008603196596E-4
94-95	4.6062231917810235E-4
96-97	2.4566523689498795E-4
98-99	1.2283261844749397E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3256464.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.77854507640017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.43419695932418	48.11083331072306
2	14.846445538133182	18.93769391956295
3	4.762369688925389	9.112110296268295
4	1.9838259854531366	5.061021401478275
5	0.9771861363728851	3.1161755023345687
6	0.5540536289255841	2.120204060830408
7	0.33708876015771966	1.504932147912688
8	0.22017789704059731	1.1234100744984585
9	0.15122139734457557	0.8680212636351511
>10	0.698648863741096	7.493393624579467
>50	0.026595742137083815	1.1557942121205147
>100	0.007707730551988191	0.8365014077532688
>500	2.8900313648849805E-4	0.11965569532418274
>1k	1.4450156703080814E-4	0.26299676529072286
>5k	4.816718901026937E-5	0.17725631768805877
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5770	0.17718605211051006	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4796	0.14727630951854526	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.070815461187349E-5	0.0
5	0.0	0.0	0.0	3.070815461187349E-5	0.0
6	0.0	0.0	0.0	3.070815461187349E-5	0.0
7	0.0	0.0	0.0	3.070815461187349E-5	0.0
8	0.0	0.0	0.0	3.070815461187349E-5	0.0
9	0.0	0.0	0.0	2.7637339150686144E-4	0.0
10-11	0.0	0.0	0.0	3.224356234246717E-4	0.0
12-13	0.0	0.0	0.0	3.8385193264841867E-4	0.0
14-15	0.0	0.0	0.0	5.066845510959127E-4	0.0
16-17	0.0	0.0	0.0	5.681008603196597E-4	0.0
18-19	0.0	0.0	0.0	6.295171695434067E-4	0.0
20-21	0.0	0.0	0.0	6.909334787671537E-4	0.0
22-23	0.0	0.0	0.0	7.369957106849638E-4	0.0
24-25	0.0	0.0	0.0	8.44474251826521E-4	0.0
26-27	0.0	0.0	0.0	9.67306870274015E-4	0.0
28-29	0.0	0.0	0.0	0.0014893454986758645	0.0
30-31	0.0	0.0	0.0	0.0037463948626485664	0.0
32-33	0.0	0.0	0.0	0.010548251109178544	0.0
34-35	0.0	0.0	0.0	0.020405568739589937	0.0
36-37	0.0	0.0	0.0	0.03731040785342629	0.0
38-39	0.0	0.0	0.0	0.06537766116867866	0.0
40-41	0.0	0.0	0.0	0.100492435967356	0.0
42-43	0.0	0.0	0.0	0.13694301549164983	0.0
44-45	0.0	0.0	0.0	0.17878287615032748	0.0
46-47	0.0	0.0	0.0	0.231570193928138	0.0
48-49	0.0	0.0	0.0	0.29274083791499	0.0
50-51	0.0	0.0	0.0	0.3598535098192395	0.0
52-53	0.0	0.0	0.0	0.4267819327958178	0.0
54-55	0.0	0.0	0.0	0.5013873944253644	0.0
56-57	0.0	0.0	0.0	0.5878615578124002	0.0
58-59	0.0	0.0	0.0	0.6863579637299844	0.0
60-61	0.0	0.0	0.0	0.7930995091608567	0.0
62-63	0.0	0.0	0.0	0.9053378142672543	0.0
64-65	0.0	0.0	0.0	1.0240708940740633	0.0
66-67	0.0	0.0	0.0	1.1603383301642518	0.0
68-69	0.0	0.0	0.0	1.3179939959416103	0.0
70-71	0.0	0.0	0.0	1.4887773978155447	0.0
72-73	0.0	0.0	0.0	1.6708921087412605	0.0
74-75	0.0	0.0	0.0	1.854787892634465	0.0
76-77	0.0	0.0	0.0	2.0544062516889485	0.0
78-79	0.0	0.0	0.0	2.2888937203052144	0.0
80-81	0.0	0.0	0.0	2.5457060173243127	0.0
82-83	0.0	0.0	0.0	2.813312230689484	0.0
84-85	0.0	0.0	0.0	3.0809952144411854	0.0
86-87	0.0	0.0	0.0	3.368960934314029	0.0
88	0.0	0.0	0.0	3.6050452269701125	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4635	0.0	49.51867	1
GTATCAA	8720	0.0	45.453697	1
TCAACGC	10120	0.0	38.684666	4
ATCAACG	10170	0.0	38.49684	3
CAACGCA	10255	0.0	38.12958	5
AACGCAG	10600	0.0	36.977245	6
TATCAAC	10980	0.0	35.954857	2
ACGCAGA	12320	0.0	31.471512	7
CGCAGAG	12515	0.0	30.830935	8
GCAGAGT	14045	0.0	27.03734	9
GTACATG	8255	0.0	26.775986	1
TACATGG	8585	0.0	24.69381	2
ACATGGG	8815	0.0	23.353546	3
GTGGTAT	2300	0.0	22.949852	1
GAGTACT	9495	0.0	22.743725	12-13
CAGAGTA	13815	0.0	21.415056	10-11
AGAGTAC	12995	0.0	21.301657	10-11
GGTCGAC	210	9.543801E-7	20.141768	7
AGTACTT	10205	0.0	20.010035	12-13
GTACTTT	10715	0.0	20.000784	14-15
>>END_MODULE