FastQCFastQC Report
Wed 25 May 2016
SRR1033027_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033027_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239228
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17210.719397394953768No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGA250.005228398456.4070361
GCGTTAG503.0000061E-547.0058637
AGCGTTA555.266613E-542.73266
TAACAAC1450.042.143191
CAAAGCG608.807496E-539.1633533
CGGTCCG500.001707040137.5653894
GGATATA651.4091075E-436.1583561
ATAGCAC550.002713848634.178933
TACCCAT702.1809299E-433.568592
GCCGTAT702.1916776E-433.54051694
GTACCTA753.2654518E-431.337246
ATTCCGC300.00416882331.31757746-47
AGGCCCG600.004173228531.30448294
GTACCCA953.7368016E-529.6879121
ATACACC804.77205E-429.3725174
TAGCACT1154.1808853E-628.6062764
ACCTATC1005.2489017E-528.2448988
GGAAACA2700.027.8553261
GAACAAA4050.027.8553261
CGTTAGG856.7372073E-427.6910768
Produced by FastQC (version 0.11.5)