##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033027_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 239228 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.034448308726404 33.0 31.0 34.0 22.0 34.0 2 29.995957831023123 33.0 31.0 34.0 23.0 34.0 3 30.18135836942164 34.0 31.0 34.0 23.0 34.0 4 33.20480880164529 37.0 35.0 37.0 23.0 37.0 5 33.62391526075543 37.0 35.0 37.0 28.0 37.0 6 33.49076613105489 37.0 35.0 37.0 26.0 37.0 7 33.57144648619727 37.0 35.0 37.0 27.0 37.0 8 33.35665139532162 37.0 35.0 37.0 25.0 37.0 9 34.71783821291822 39.0 37.0 39.0 18.0 39.0 10-11 34.89141948266925 39.0 37.0 39.0 24.5 39.0 12-13 34.86180756433193 39.0 37.0 39.0 24.5 39.0 14-15 36.15364840236093 40.0 38.0 41.0 24.5 41.0 16-17 36.22720793552594 40.0 38.0 41.0 27.0 41.0 18-19 36.25455214272577 40.0 38.0 41.0 27.0 41.0 20-21 36.304742755864694 40.0 38.0 41.0 27.0 41.0 22-23 36.294300834350494 40.0 37.5 41.0 27.0 41.0 24-25 36.30402586653736 40.0 37.0 41.0 27.0 41.0 26-27 36.113688615045064 40.0 37.0 41.0 27.0 41.0 28-29 35.99523049141405 40.0 37.0 41.0 27.0 41.0 30-31 35.958662865550856 40.0 37.0 41.0 27.0 41.0 32-33 35.78222657882856 40.0 37.0 41.0 26.5 41.0 34-35 35.59909584162389 40.0 37.0 41.0 25.0 41.0 36-37 35.38611073954554 40.0 37.0 41.0 23.5 41.0 38-39 35.186554249502564 40.0 36.5 41.0 18.0 41.0 40-41 35.037445449529315 40.0 36.0 41.0 11.0 41.0 42-43 34.85164989048104 40.0 36.0 41.0 2.0 41.0 44-45 34.67312981757988 40.0 35.0 41.0 2.0 41.0 46-47 34.50177236778303 40.0 35.0 41.0 2.0 41.0 48-49 34.3413062016152 40.0 35.0 41.0 2.0 41.0 50-51 34.16705402377649 40.0 35.0 41.0 2.0 41.0 52-53 33.989415954654135 40.0 35.0 41.0 2.0 41.0 54-55 33.80611592288528 39.0 35.0 41.0 2.0 41.0 56-57 33.598773972946304 39.0 34.5 41.0 2.0 41.0 58-59 33.376630243951375 39.0 34.0 41.0 2.0 41.0 60-61 33.13070376377347 38.0 34.0 41.0 2.0 41.0 62-63 32.87397587238952 37.5 34.0 40.5 2.0 41.0 64-65 32.59307856939823 37.0 33.5 40.0 2.0 41.0 66-67 32.27967252997141 36.5 33.0 40.0 2.0 41.0 68-69 31.959103867440266 36.0 33.0 39.5 2.0 41.0 70-71 31.62497909943652 35.5 33.0 39.0 2.0 41.0 72-73 31.29069966726303 35.0 32.0 39.0 2.0 41.0 74-75 30.98093659605063 35.0 32.0 37.5 2.0 40.0 76-77 30.64053747889043 35.0 32.0 37.0 2.0 39.5 78-79 30.287217633387396 35.0 31.0 37.0 2.0 39.0 80-81 29.97028566890163 35.0 31.0 36.0 2.0 39.0 82-83 29.76997884862976 35.0 31.0 36.0 2.0 37.5 84-85 29.595603357466516 35.0 31.0 35.5 2.0 37.0 86-87 29.455239353252963 35.0 31.0 35.0 2.0 37.0 88-89 29.24128864514187 35.0 31.0 35.0 2.0 36.0 90-91 29.02488839099102 35.0 31.0 35.0 2.0 36.0 92-93 28.792883776146603 35.0 31.0 35.0 2.0 36.0 94-95 28.567728275954323 35.0 30.0 35.0 2.0 35.5 96-97 28.371990318858998 35.0 30.0 35.0 2.0 35.0 98-99 28.172463089604896 35.0 30.0 35.0 2.0 35.0 100 28.01621047703446 35.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7580438582305078 1101 2 0.9110286674818688 1101 3 0.8588208686256067 1101 4 0.7986758754617753 1101 5 0.6625753735169653 1101 6 0.8387513883671929 1101 7 1.0058956001786683 1101 8 1.1861939136252033 1101 9 1.2420790616867095 1101 10-11 1.212933254114887 1101 12-13 1.568479759076638 1101 14-15 1.7841844994979326 1101 16-17 1.795678821877864 1101 18-19 1.5271791251239577 1101 20-21 1.4122463308016435 1101 22-23 1.4222723372451043 1101 24-25 1.4657622033654647 1101 26-27 1.3065469950093345 1101 28-29 1.2995934643889058 1101 30-31 1.2100188639432616 1101 32-33 1.2527376979793274 1101 34-35 1.2470559052889882 1101 36-37 1.0485508749202666 1101 38-39 1.204384077649813 1101 40-41 0.926173992978228 1101 42-43 1.0476727422659806 1101 44-45 1.1613772418401282 1101 46-47 1.017917162483414 1101 48-49 0.9544713458606111 1101 50-51 1.2458005262924772 1101 52-53 1.0717762602762875 1101 54-55 1.2510838157124269 1101 56-57 1.3206042122041026 1101 58-59 1.4778713713535652 1101 60-61 1.5874568507056281 1101 62-63 1.4470316512824013 1101 64-65 0.987513957896148 1101 66-67 1.1791685093349002 1101 68-69 1.206087195114577 1101 70-71 1.6254681123030323 1101 72-73 1.3160445228712838 1101 74-75 1.105022788548169 1101 76-77 1.2322538793060644 1101 78-79 1.0955436941858352 1101 80-81 0.7554372502904236 1101 82-83 0.9053710668003667 1101 84-85 0.913956930544245 1101 86-87 0.8371248104966895 1101 88-89 0.9606069441128753 1101 90-91 0.9050382472760639 1101 92-93 0.5956568038166452 1101 94-95 0.848026091424142 1101 96-97 0.7446743525075057 1101 98-99 0.7599569887017914 1101 100 0.8165186174127648 1102 1 0.3389262111716853 1102 2 0.422670824344614 1102 3 0.3447277313707069 1102 4 0.5588882937624291 1102 5 0.03982210554310939 1102 6 0.47171707464170254 1102 7 0.10046259364272458 1102 8 0.4339471815990592 1102 9 0.3366460551507586 1102 10-11 0.3768425678403844 1102 12-13 0.5242599878348102 1102 14-15 0.5836126040731031 1102 16-17 0.5269900963876708 1102 18-19 0.5999936676076203 1102 20-21 0.5426589125009897 1102 22-23 0.3941881869183135 1102 24-25 0.35271073277722564 1102 26-27 0.32717607997665255 1102 28-29 0.3707740199444629 1102 30-31 0.4608358574073108 1102 32-33 0.33413067183553835 1102 34-35 0.270646591563505 1102 36-37 0.4971824108679854 1102 38-39 0.4220107116367444 1102 40-41 0.44016500605012965 1102 42-43 0.22741130435748858 1102 44-45 0.23439603268980136 1102 46-47 0.2666916722873225 1102 48-49 0.32354814324622794 1102 50-51 0.1261109838088217 1102 52-53 0.23588982236778833 1102 54-55 0.22075293335948487 1102 56-57 0.29772839521064043 1102 58-59 0.17466467200716096 1102 60-61 0.034556523908243264 1102 62-63 0.0709082852693328 1102 64-65 0.185124252013793 1102 66-67 0.042219979923139306 1102 68-69 0.22038458073549094 1102 70-71 0.15712660903505338 1102 72-73 -0.01473570588688844 1102 74-75 -0.11607606766098044 1102 76-77 0.03321384662632809 1102 78-79 -0.07469731888605935 1102 80-81 0.06395440715317235 1102 82-83 0.160987896865727 1102 84-85 0.018225721393921646 1102 86-87 0.212839679777737 1102 88-89 0.09786184607365911 1102 90-91 0.1689271361649496 1102 92-93 0.22331203257481747 1102 94-95 0.16001547050910858 1102 96-97 0.18595294727874645 1102 98-99 0.40119473379983006 1102 100 0.3796313625108034 1103 1 0.4804009240341749 1103 2 0.70279646683462 1103 3 0.3161091642027394 1103 4 0.7287594784822744 1103 5 0.3950794188244089 1103 6 0.248035368949715 1103 7 0.4910965171150252 1103 8 0.24751403228755464 1103 9 0.5519833227438795 1103 10-11 0.35888457558198894 1103 12-13 0.5521805411694061 1103 14-15 0.3572022988506873 1103 16-17 0.2225767452867089 1103 18-19 0.4565042329988245 1103 20-21 0.5871890222795244 1103 22-23 0.4971610912904154 1103 24-25 0.5042431904204747 1103 26-27 0.6963460780729775 1103 28-29 0.6124137178948388 1103 30-31 0.7441342900489758 1103 32-33 0.9859328166416788 1103 34-35 0.7497696323655845 1103 36-37 0.7915221532374233 1103 38-39 0.9811673840144337 1103 40-41 0.8595444082964647 1103 42-43 0.9244257088247707 1103 44-45 1.0186621663277222 1103 46-47 1.0636965583841658 1103 48-49 0.8253249057311578 1103 50-51 0.7366985845449037 1103 52-53 0.7516744533722957 1103 54-55 0.5620499699734864 1103 56-57 0.635329131297965 1103 58-59 0.858686500803401 1103 60-61 0.7658211980626888 1103 62-63 0.8123734797613622 1103 64-65 0.6419267140989504 1103 66-67 0.7642042428839915 1103 68-69 0.7433714453842626 1103 70-71 0.886154466132588 1103 72-73 0.9764915293437788 1103 74-75 0.8180250808890541 1103 76-77 0.7522950065984055 1103 78-79 0.8770128150056848 1103 80-81 1.121048765932283 1103 82-83 0.8598107917194611 1103 84-85 1.1085867849131787 1103 86-87 0.9257338989540784 1103 88-89 1.074684614015787 1103 90-91 0.8933068556903017 1103 92-93 1.1646630066213959 1103 94-95 1.146182105447874 1103 96-97 0.9279099242442932 1103 98-99 1.0830590652929466 1103 100 1.1877092862369274 1104 1 0.09679724576438531 1104 2 0.2502054967501621 1104 3 0.16327547567167855 1104 4 0.23448210852411222 1104 5 0.28274813774460483 1104 6 -0.058352270169393705 1104 7 0.177220125923931 1104 8 -0.07862654707940209 1104 9 0.03975860098210404 1104 10-11 -0.18776965916424615 1104 12-13 0.12314608698991236 1104 14-15 0.2816438490914308 1104 16-17 0.28521981103775573 1104 18-19 0.3368420655033688 1104 20-21 0.14907729286125715 1104 22-23 0.12590546732641172 1104 24-25 0.18819614645489224 1104 26-27 0.3836980790201707 1104 28-29 0.3120003072073416 1104 30-31 0.36872482600287526 1104 32-33 0.5800158145104959 1104 34-35 0.3586328023079588 1104 36-37 0.22017180987961638 1104 38-39 0.4074243893029319 1104 40-41 0.4721174211625154 1104 42-43 0.5397120376589299 1104 44-45 0.45791348845259705 1104 46-47 0.47709737928558127 1104 48-49 0.553810776754915 1104 50-51 0.33878833117052665 1104 52-53 0.4409751085147704 1104 54-55 0.37942900541433033 1104 56-57 0.4293440496024772 1104 58-59 0.3463927128169857 1104 60-61 0.1652854414915339 1104 62-63 0.45401843990028823 1104 64-65 0.7174851638582069 1104 66-67 0.31411939009371537 1104 68-69 0.28874302167284327 1104 70-71 0.2729105242271501 1104 72-73 0.10910854726152763 1104 74-75 0.13089507852107118 1104 76-77 0.3155448684659561 1104 78-79 0.36811313863027806 1104 80-81 0.32119775164543896 1104 82-83 0.5680997253369533 1104 84-85 0.30712258095075384 1104 86-87 0.2334239297378815 1104 88-89 0.08865572460068094 1104 90-91 0.03664393833297197 1104 92-93 -0.05991568940828529 1104 94-95 0.1731869992832209 1104 96-97 0.14715572107119002 1104 98-99 0.205375349135398 1104 100 0.1279176689032795 1105 1 0.6431745771847552 1105 2 0.6216904321877514 1105 3 0.6009368816975034 1105 4 0.6781693395140636 1105 5 0.6509332166542166 1105 6 0.4854425648377827 1105 7 0.6681096524662564 1105 8 0.8234896652591885 1105 9 0.7565806956736338 1105 10-11 1.0006987769907028 1105 12-13 1.1343907067890484 1105 14-15 1.2457041073410764 1105 16-17 0.998885521224274 1105 18-19 1.0700917068233124 1105 20-21 1.1733778667493553 1105 22-23 1.2847110627353047 1105 24-25 1.2772205366987919 1105 26-27 1.1634505897805738 1105 28-29 1.1086825166764953 1105 30-31 1.0077312822439097 1105 32-33 1.059947665299589 1105 34-35 1.0605158726092512 1105 36-37 0.929371953351648 1105 38-39 0.9009322802641933 1105 40-41 0.922027751148164 1105 42-43 1.0316596703705585 1105 44-45 0.9054744640623511 1105 46-47 0.9434890579082449 1105 48-49 0.7227311497821773 1105 50-51 0.721535820410125 1105 52-53 0.6131774040671374 1105 54-55 0.7042496653856247 1105 56-57 1.0563885259295986 1105 58-59 0.9740110772359216 1105 60-61 0.7557983539736028 1105 62-63 0.6344703767725903 1105 64-65 0.866660199726212 1105 66-67 0.9217951433218303 1105 68-69 0.889012031715886 1105 70-71 0.6881723606690358 1105 72-73 0.7904930522830433 1105 74-75 0.8296755663259461 1105 76-77 0.7828870492407027 1105 78-79 0.6269366680420418 1105 80-81 0.7799674790485973 1105 82-83 0.9073931256238943 1105 84-85 0.8886505579952271 1105 86-87 0.9522187647450551 1105 88-89 0.9164892970540528 1105 90-91 0.9349402080603753 1105 92-93 1.0059917711369089 1105 94-95 1.2091984770450566 1105 96-97 1.2531098100238474 1105 98-99 1.1381228383749935 1105 100 1.115579074929105 1106 1 0.0844743306190665 1106 2 0.014134954907017772 1106 3 0.056695951792605115 1106 4 -0.15795253438519552 1106 5 -0.20200795981637043 1106 6 0.043279831480965925 1106 7 -0.5384947857162032 1106 8 -0.2796452413980859 1106 9 -0.1073678777677145 1106 10-11 0.01219930534576008 1106 12-13 -0.11140006460932739 1106 14-15 0.2513751182604125 1106 16-17 0.1424748132284961 1106 18-19 -0.11404883562121171 1106 20-21 0.1831524352593945 1106 22-23 0.28832148894171894 1106 24-25 0.22003490795628267 1106 26-27 0.39360205156288686 1106 28-29 0.6224373599311548 1106 30-31 0.4903748187004098 1106 32-33 0.4912086726965583 1106 34-35 0.21962119138346736 1106 36-37 0.4149742781138883 1106 38-39 0.29708409428321403 1106 40-41 0.7130897448079025 1106 42-43 0.5064219105959893 1106 44-45 0.5412529071761227 1106 46-47 0.5617877546324408 1106 48-49 0.599369050451422 1106 50-51 0.7042586101248176 1106 52-53 0.6700900493647808 1106 54-55 0.6263437125853457 1106 56-57 0.5336780644995116 1106 58-59 0.5655397845272176 1106 60-61 0.4441393191021703 1106 62-63 0.7262544556736188 1106 64-65 0.8196101821143742 1106 66-67 0.41009207886251886 1106 68-69 0.37046676929954003 1106 70-71 0.21406175834427899 1106 72-73 0.48286712766170226 1106 74-75 0.3712923327930113 1106 76-77 0.09757531975849432 1106 78-79 0.07619697096560785 1106 80-81 0.16323623565303436 1106 82-83 0.049925707518927 1106 84-85 -0.0817703648649335 1106 86-87 0.08805004336429079 1106 88-89 0.12527760279551003 1106 90-91 -0.012875924380626458 1106 92-93 0.17198331745603213 1106 94-95 -0.107136500059557 1106 96-97 -0.25175986239602466 1106 98-99 -0.2824477850839706 1106 100 -0.3843504777302833 1107 1 1.0618187870054356 1107 2 0.996409721612924 1107 3 1.4141093301640701 1107 4 1.5314037956896982 1107 5 1.021638763830353 1107 6 1.4068603627261638 1107 7 1.52379089932397 1107 8 1.4763470475283356 1107 9 1.4764985773562245 1107 10-11 1.5585083965650313 1107 12-13 1.713467294689167 1107 14-15 1.6864280892415238 1107 16-17 1.6852354458094894 1107 18-19 1.3846844670042984 1107 20-21 1.481112071542377 1107 22-23 1.5032463400941083 1107 24-25 1.437761847240104 1107 26-27 1.6009736331859656 1107 28-29 1.7468423960128376 1107 30-31 1.7360160149404038 1107 32-33 1.6364182535348846 1107 34-35 1.601801275659362 1107 36-37 1.7685918293362306 1107 38-39 1.698908650299387 1107 40-41 1.7074685086477501 1107 42-43 1.6609206055138372 1107 44-45 1.8086795922674739 1107 46-47 1.7100467921130402 1107 48-49 1.8811985538378124 1107 50-51 1.8784572214491746 1107 52-53 1.8531598072848325 1107 54-55 1.8230656533192686 1107 56-57 1.8683250099249022 1107 58-59 1.7129586220658197 1107 60-61 1.8021114091101893 1107 62-63 1.8520797282054744 1107 64-65 1.812700923708114 1107 66-67 1.732809891101283 1107 68-69 1.8472908988182795 1107 70-71 2.0219246650095855 1107 72-73 1.9263988676006285 1107 74-75 1.9125815492319305 1107 76-77 2.088601813778997 1107 78-79 1.8289393494564905 1107 80-81 2.007351424079598 1107 82-83 2.0015694286237284 1107 84-85 1.8282642667265847 1107 86-87 1.9315425455394255 1107 88-89 1.8017750352809685 1107 90-91 1.6988416660794812 1107 92-93 1.6496988266086703 1107 94-95 1.5984267324497807 1107 96-97 1.3283192955274465 1107 98-99 1.486497230867034 1107 100 1.3523982470423945 1108 1 0.1118940220672684 1108 2 0.19697035648991346 1108 3 0.3630564212023124 1108 4 0.4112950294683344 1108 5 0.20147729487531763 1108 6 0.3118579567318065 1108 7 0.38133311879648346 1108 8 0.29925058624957046 1108 9 0.02755530535664974 1108 10-11 0.414568829032973 1108 12-13 0.4045452565446084 1108 14-15 0.5301071983361894 1108 16-17 0.39359455765386286 1108 18-19 0.36571148288386013 1108 20-21 0.3598232223714959 1108 22-23 0.4391819663782641 1108 24-25 0.3215905098891412 1108 26-27 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-0.9837684742902155 2215 26-27 -0.668094335881996 2215 28-29 -0.70773635856591 2215 30-31 -0.8260922471678498 2215 32-33 -0.855889438772806 2215 34-35 -1.0075190336609552 2215 36-37 -0.7905860298416343 2215 38-39 -1.012690861300122 2215 40-41 -0.8542327530535161 2215 42-43 -0.9031819585887177 2215 44-45 -0.8689760853882049 2215 46-47 -0.6802665982003475 2215 48-49 -0.6769618043225449 2215 50-51 -0.5485676055756556 2215 52-53 -0.6503810962560621 2215 54-55 -0.5886559584023487 2215 56-57 -0.7231686815687866 2215 58-59 -0.7212701121629195 2215 60-61 -0.6008596938609188 2215 62-63 -0.8164184402926864 2215 64-65 -1.0840023729951866 2215 66-67 -0.8981628398714427 2215 68-69 -0.9280855765581961 2215 70-71 -0.9771799340950196 2215 72-73 -1.1578909166891584 2215 74-75 -0.8846101479597692 2215 76-77 -0.7995951744179912 2215 78-79 -0.5928758723587322 2215 80-81 -0.4784035616753819 2215 82-83 -0.45366052660622813 2215 84-85 -0.38007924161692586 2215 86-87 -0.53561252161564 2215 88-89 -0.5619205284145963 2215 90-91 -0.5478005805627681 2215 92-93 -0.5274277505179086 2215 94-95 -0.5355171014940545 2215 96-97 -0.3562146890065385 2215 98-99 -0.3572553128855098 2215 100 -0.30555861091446346 2216 1 -0.0681699606303674 2216 2 -0.38965410562737546 2216 3 -0.09948328636972548 2216 4 -0.1306541898976974 2216 5 -0.17679787578275352 2216 6 -0.3385070149941498 2216 7 -0.3806298433320663 2216 8 -0.29667840196770356 2216 9 -0.22871342197342415 2216 10-11 -0.5169482818328248 2216 12-13 -0.3817857637991864 2216 14-15 -0.4677412708101869 2216 16-17 -0.47044220986816043 2216 18-19 -0.46478224275652025 2216 20-21 -0.48208431812459906 2216 22-23 -0.5640284330630152 2216 24-25 -0.5145861859902823 2216 26-27 -0.8846586539169081 2216 28-29 -0.5799729455083877 2216 30-31 -0.7183191379241549 2216 32-33 -0.9444640994062894 2216 34-35 -1.1646942114243615 2216 36-37 -0.9445776264802888 2216 38-39 -0.9778492323408514 2216 40-41 -0.9596949379274591 2216 42-43 -0.8309453716462514 2216 44-45 -0.7859120905594992 2216 46-47 -0.8298302698228497 2216 48-49 -1.0995377577808512 2216 50-51 -1.0467414905142434 2216 52-53 -1.0920746967731958 2216 54-55 -1.1417587379757137 2216 56-57 -1.3394097933981328 2216 58-59 -1.1648544689825684 2216 60-61 -1.0198318981272436 2216 62-63 -1.139059034992112 2216 64-65 -0.8734658506939574 2216 66-67 -0.9256838482748009 2216 68-69 -1.0051056153429414 2216 70-71 -0.8674999082385213 2216 72-73 -0.6998430821707338 2216 74-75 -0.7678034252706922 2216 76-77 -0.6958944633643362 2216 78-79 -0.6970129117898907 2216 80-81 -0.7286459663295553 2216 82-83 -0.9001698995861851 2216 84-85 -0.93088725713082 2216 86-87 -0.6066047646667094 2216 88-89 -0.6549392743745202 2216 90-91 -0.6698917247127341 2216 92-93 -0.9267813381067889 2216 94-95 -0.7977342960641884 2216 96-97 -0.6708397698080866 2216 98-99 -0.6885578338939133 2216 100 -0.6684545385162757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7948.0 3 1128.0 4 758.0 5 870.0 6 1632.0 7 3293.0 8 3795.0 9 2324.0 10 1407.0 11 1395.0 12 1335.0 13 954.0 14 821.0 15 755.0 16 734.0 17 675.0 18 599.0 19 472.0 20 421.0 21 434.0 22 605.0 23 1040.0 24 1721.0 25 1654.0 26 1569.0 27 1298.0 28 1481.0 29 1956.0 30 2382.0 31 2959.0 32 3880.0 33 5213.0 34 7745.0 35 13171.0 36 23865.0 37 46401.0 38 63790.0 39 26727.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.79247895135284 20.376512512077092 13.318889618510262 22.5121189180598 2 15.703066848430613 24.7271449957765 36.623205011332374 22.94658314446052 3 18.641756899746134 28.944429806401423 28.53456460186614 23.879248691986298 4 12.862772000568889 20.281349608051467 34.10496021952464 32.750918171855 5 12.487763247069454 41.71331275048738 31.489662558464488 14.309261443978679 6 28.71832629821912 39.22163328996523 16.758771581206414 15.30126883060923 7 25.048841811100374 37.71006162225932 19.904449938712418 17.336646627927895 8 23.470194685377884 38.32536265538638 19.56686373697181 18.637578922263923 9 23.959283401250115 21.656527960237305 18.90861776100545 35.47557087750713 10-11 21.953255425709518 31.311375266212277 25.75721440496065 20.97815490311756 12-13 22.91901594826594 30.12471154831867 25.68124807786051 21.275024425554882 14-15 21.418065798420418 31.47863381976045 23.15267534912914 23.950625032689995 16-17 20.538599415638874 33.908003906895416 23.970518416577953 21.582878260887753 18-19 20.77719947115626 32.732875336803176 25.90602145498971 20.58390373705086 20-21 22.674559678701417 30.914529556959376 25.540517926620087 20.870392837719116 22-23 22.487238843267274 30.81706952399474 25.24816555104567 21.447526081692313 24-25 22.004749422000362 31.0905274952234 24.893075292553522 22.011647790222714 26-27 22.065939035599207 30.767153173109335 25.450198168848654 21.716709622442806 28-29 21.216962862890913 31.109386959558655 25.906678322117056 21.76697185543338 30-31 21.766174877331537 31.700124237113016 25.59326358764991 20.940437297905536 32-33 21.454849673202613 31.783529411764704 25.573437908496732 21.188183006535947 34-35 21.397096020301937 31.346341177896974 25.63788468533252 21.61867811646857 36-37 21.458652213551467 31.84348313385023 25.228966451710033 21.468898200888265 38-39 21.13774546610586 31.94609732682731 25.74829384193541 21.16786336513142 40-41 20.90403870328112 30.991070886992024 26.383447779643966 21.72144263008289 42-43 20.762980392156862 31.58650980392157 26.08962091503268 21.560888888888886 44-45 20.803486837564652 31.817601920772326 25.88608799496383 21.492823246699196 46-47 20.842771478709007 31.50196710819409 25.8961793550897 21.759082058007206 48-49 20.741741578438795 31.24793461028449 25.710598638829747 22.29972517244697 50-51 20.33036556468125 30.64121789361626 26.06114983780799 22.96726670389449 52-53 20.726299982012957 30.312765361142134 25.575271583403396 23.385663073441506 54-55 20.599128745338913 30.75282174832326 25.39144765047756 23.256601855860264 56-57 21.101151511856013 30.543506903633556 25.45393325163023 22.9014083328802 58-59 21.326203476955186 30.053306620770133 25.28477948542067 23.33571041685401 60-61 21.216474795319805 30.240179010215712 25.248805378671435 23.29454081579305 62-63 21.262953164702562 30.184977988769568 25.312601298714878 23.239467547812996 64-65 21.634129764207664 30.5534584618602 25.06007214931772 22.75233962461442 66-67 21.613680375762797 30.544948073295497 24.956396625496158 22.88497492544555 68-69 21.994428666435226 31.006437148916056 24.80519154596141 22.193942638687307 70-71 22.40844987955032 30.668453894539617 24.93747490631692 21.98562131959315 72-73 22.23272406387815 30.549346225505676 25.051074498717124 22.16685521189905 74-75 22.46812016679144 30.901376405786674 24.753556028623887 21.876947398797995 76-77 22.927574900884924 30.03563124174041 24.963615985546763 22.073177871827898 78-79 23.327053401722555 29.55192234909868 25.2781535746472 21.842870674531568 80-81 23.35388687814027 29.07877046729519 25.623349495988606 21.943993158575932 82-83 23.0819415721691 29.285219904188565 25.522812197452694 22.110026326189644 84-85 23.237482724518536 29.075915940277536 25.595102440207494 22.091498894996437 86-87 23.404851409384186 28.83163911736296 25.575482862005376 22.188026611247473 88-89 23.27288617911677 28.761638324875747 25.492557535612804 22.47291796039467 90-91 23.184163648984303 28.302375954437874 25.642494543082467 22.870965853495353 92-93 23.47883902100417 28.466649661499606 25.69822240249558 22.35628891500065 94-95 23.039867526406905 28.568172884729577 25.828169037642905 22.563790551220613 96-97 22.668880209422333 28.830488345446486 25.53714653708799 22.96348490804319 98-99 22.247010586048496 29.87929437494642 25.128013197538635 22.74568184146645 100 22.191135757241195 30.359069580709107 24.956715931046595 22.493078731003102 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1302.0 1 860.5 2 378.0 3 338.0 4 289.5 5 207.5 6 167.0 7 172.0 8 197.0 9 217.0 10 178.5 11 118.0 12 106.5 13 102.5 14 113.0 15 142.0 16 163.5 17 203.5 18 269.0 19 334.0 20 413.0 21 504.0 22 662.5 23 913.0 24 1261.0 25 1671.0 26 1961.0 27 2288.5 28 2985.5 29 3698.5 30 4318.0 31 5071.0 32 5817.5 33 6104.0 34 6383.5 35 7028.0 36 7255.0 37 7093.5 38 7085.0 39 7187.5 40 6872.5 41 6324.0 42 5963.5 43 5837.0 44 5961.5 45 5891.0 46 5844.0 47 6037.5 48 6416.5 49 6972.0 50 7454.5 51 7840.5 52 7563.5 53 7316.5 54 7197.0 55 6909.0 56 6456.0 57 5798.5 58 5319.5 59 4939.0 60 4410.0 61 3792.0 62 3298.0 63 3039.0 64 2613.0 65 2045.0 66 1731.5 67 1211.0 68 800.5 69 622.5 70 554.5 71 455.5 72 275.0 73 160.5 74 96.0 75 65.0 76 52.5 77 39.5 78 27.5 79 29.0 80 26.5 81 18.5 82 14.0 83 9.5 84 6.0 85 5.0 86 3.0 87 1.5 88 1.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05810356647215209 2 0.037621014262544515 3 0.0522514086979785 4 0.06938987075091545 5 0.08109418629926263 6 0.07900412995134348 7 0.07984015249051114 8 0.09488855819552895 9 0.08778236661260388 10-11 0.09488855819552895 12-13 0.09885966525657532 14-15 0.10053171033491064 16-17 0.06834484257695587 18-19 0.09029043423010684 20-21 0.08276623137759795 22-23 0.05162439179360275 24-25 0.017138462052936947 26-27 0.17410169378166435 28-29 0.05893958901131975 30-31 0.07064390455966692 32-33 0.06918086511612354 34-35 0.016302439513769293 36-37 0.04598123965422108 38-39 0.07001688765529118 40-41 0.0746150116207133 42-43 0.06918086511612354 44-45 0.0658367749594529 46-47 0.07168893273362649 48-49 0.07064390455966692 50-51 0.06667279749862055 52-53 0.06980788202049927 54-55 0.061656662263614624 56-57 0.06311970170715803 58-59 0.058312572106944 60-61 0.05664052702860869 62-63 0.06082063972444698 64-65 0.058730583376527826 66-67 0.05893958901131975 68-69 0.06144765662882271 70-71 0.05183339742839466 72-73 0.04869831290651596 74-75 0.053087431237146154 76-77 0.046817262193388734 78-79 0.04451820021067768 80-81 0.0403380875148394 82-83 0.047026267828180646 84-85 0.03699399735816878 86-87 0.03385891283629006 88-89 0.03908405370608792 90-91 0.03427692410587389 92-93 0.03553095791462538 94-95 0.03594896918420921 96-97 0.03929305934087983 98-99 0.03866604243650409 100 0.04598123965422108 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 239228.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.51248059342919 #Duplication Level Percentage of deduplicated Percentage of total 1 78.713621381206 38.18593029694288 2 9.575342303961719 9.290472153927688 3 3.054076040567979 4.444824139467334 4 1.7043708935522637 3.3073303958983904 5 1.1840590071368997 2.872081980260194 6 0.8978199609643328 2.6133284059605324 7 0.7150216422298543 2.4281231479790373 8 0.5493504693876571 2.1320283188127944 9 0.4343115588697783 1.896257796405489 >10 3.021308446645377 26.594459305177143 >50 0.13088694080998692 4.188844979728388 >100 0.01883978693477084 1.2184593860418014 >500 0.0 0.0 >1k 9.915677334089916E-4 0.8278596933983182 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1721 0.719397394953768 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 8.36022539167656E-4 0.0 16-17 0.0 0.0 0.0 0.001463039443543398 0.0 18-19 0.0 0.0 0.0 0.001881050713127226 0.0 20-21 0.0 0.0 0.0 0.002717073252294882 0.0 22-23 0.0 0.0 0.0 0.004807129600214022 0.0 24-25 0.0 0.0 0.0 0.008778236661260388 0.0 26-27 0.0 0.0 0.0 0.025916698714197335 0.0 28-29 0.0 0.0 0.0 0.05204240306318658 0.0 30-31 0.0 0.0 0.0 0.10178574414366212 0.0 32-33 0.0 0.0 0.0 0.1883140769475145 0.0 34-35 0.0 0.0 0.0 0.3346180213018543 0.0 36-37 0.0 0.0 0.0 0.5743474844081796 0.0 38-39 0.0 0.0 0.0 0.8778236661260388 0.0 40-41 0.0 0.0 0.0 1.1731486280870134 0.0 42-43 0.0 0.0 0.0 1.4256274349156453 0.0 44-45 0.0 0.0 0.0 1.6808233149965723 0.0 46-47 0.0 0.0 0.0 1.9928687277409 0.0 48-49 0.0 0.0 0.0 2.3199625461902453 0.0 50-51 0.0 0.0 0.0 2.6309629307606133 0.0 52-53 0.0 0.0 0.0 2.9298409885130505 0.0 54-55 0.0 0.0 0.0 3.2454394970488405 0.0 56-57 0.0 0.0 0.0 3.642968214423061 0.0 58-59 0.0 0.0 0.0 4.002039894995569 0.0 60-61 0.0 0.0 0.0 4.358812513585367 0.0 62-63 0.0 0.0 0.0 4.727498453358303 0.0 64-65 0.0 0.0 0.0 5.1066346748708344 0.0 66-67 0.0 0.0 0.0 5.558295851656161 0.0 68-69 0.0 0.0 0.0 6.090842209105958 0.0 70-71 0.0 0.0 0.0 6.641572056782651 0.0 72-73 0.0 0.0 0.0 7.172028357884528 0.0 74-75 0.0 0.0 0.0 7.730282408413731 0.0 76-77 0.0 0.0 0.0 8.331173608440483 0.0 78-79 0.0 0.0 0.0 9.019011152540672 0.0 80-81 0.0 0.0 0.0 9.727331248850469 0.0 82-83 0.0 0.0 0.0 10.474735398866354 0.0 84-85 0.0 0.0 0.0 11.212943300951395 0.0 86-87 0.0 0.0 0.0 12.028901299179026 0.0 88 0.0 0.0 0.0 12.651947096493721 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCCGA 25 0.0052283984 56.407036 1 GCGTTAG 50 3.0000061E-5 47.005863 7 AGCGTTA 55 5.266613E-5 42.7326 6 TAACAAC 145 0.0 42.14319 1 CAAAGCG 60 8.807496E-5 39.163353 3 CGGTCCG 50 0.0017070401 37.56538 94 GGATATA 65 1.4091075E-4 36.158356 1 ATAGCAC 55 0.0027138486 34.17893 3 TACCCAT 70 2.1809299E-4 33.56859 2 GCCGTAT 70 2.1916776E-4 33.540516 94 GTACCTA 75 3.2654518E-4 31.33724 6 ATTCCGC 30 0.004168823 31.317577 46-47 AGGCCCG 60 0.0041732285 31.304482 94 GTACCCA 95 3.7368016E-5 29.687912 1 ATACACC 80 4.77205E-4 29.372517 4 TAGCACT 115 4.1808853E-6 28.606276 4 ACCTATC 100 5.2489017E-5 28.244898 8 GGAAACA 270 0.0 27.855326 1 GAACAAA 405 0.0 27.855326 1 CGTTAGG 85 6.7372073E-4 27.691076 8 >>END_MODULE