Basic Statistics
Measure | Value |
---|---|
Filename | SRR1033027_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 239228 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2324 | 0.9714581905128161 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2090 | 0.8736435534302004 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT | 1304 | 0.5450866955373117 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1189 | 0.4970153995351715 | No Hit |
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 637 | 0.2662731787248984 | No Hit |
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT | 544 | 0.2273981306536024 | TruSeq Adapter, Index 10 (95% over 21bp) |
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT | 386 | 0.1613523500593576 | RNA PCR Primer, Index 10 (95% over 23bp) |
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 373 | 0.15591820355476785 | No Hit |
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 371 | 0.15508218101560017 | No Hit |
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 308 | 0.12874747103181902 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 304 | 0.1270754259534837 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG | 288 | 0.12038724564014246 | No Hit |
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT | 286 | 0.1195512231009748 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTATCA | 1780 | 0.0 | 43.454803 | 1 |
GGTATGA | 65 | 1.3814717E-4 | 36.280273 | 1 |
GCCATTA | 100 | 1.4057387E-6 | 32.89773 | 9 |
GTATCAA | 2615 | 0.0 | 31.382784 | 1 |
TACATAC | 90 | 2.5866315E-5 | 31.331173 | 5 |
GGGCCAT | 90 | 2.5866315E-5 | 31.331173 | 7 |
CGGGGCA | 65 | 0.0061466335 | 28.921083 | 9 |
ATCCCGT | 65 | 0.0061466335 | 28.921083 | 94 |
ACGTCAC | 65 | 0.0061466335 | 28.921083 | 7 |
TGGACAC | 85 | 6.8029494E-4 | 27.645153 | 5 |
TTGGACA | 70 | 0.008825259 | 26.855291 | 4 |
GGCCATT | 105 | 7.400923E-5 | 26.855291 | 8 |
TAACCTC | 105 | 7.400923E-5 | 26.855291 | 5 |
ATAGTGG | 105 | 7.400923E-5 | 26.855291 | 6 |
TATCAAC | 3140 | 0.0 | 25.518784 | 2 |
ATCAACG | 3130 | 0.0 | 25.375246 | 3 |
TCAACGC | 3165 | 0.0 | 25.094635 | 4 |
CAACGCA | 3185 | 0.0 | 24.937056 | 5 |
AACGCAG | 3265 | 0.0 | 24.1821 | 6 |
TATAAGA | 125 | 2.3692867E-4 | 22.624655 | 2 |