FastQCFastQC Report
Wed 25 May 2016
SRR1033027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033027_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239228
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23240.9714581905128161No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20900.8736435534302004No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT13040.5450866955373117No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11890.4970153995351715No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6370.2662731787248984No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT5440.2273981306536024TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT3860.1613523500593576RNA PCR Primer, Index 10 (95% over 23bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.15591820355476785No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.15508218101560017No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3080.12874747103181902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3040.1270754259534837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2880.12038724564014246No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2860.1195512231009748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17800.043.4548031
GGTATGA651.3814717E-436.2802731
GCCATTA1001.4057387E-632.897739
GTATCAA26150.031.3827841
TACATAC902.5866315E-531.3311735
GGGCCAT902.5866315E-531.3311737
CGGGGCA650.006146633528.9210839
ATCCCGT650.006146633528.92108394
ACGTCAC650.006146633528.9210837
TGGACAC856.8029494E-427.6451535
TTGGACA700.00882525926.8552914
GGCCATT1057.400923E-526.8552918
TAACCTC1057.400923E-526.8552915
ATAGTGG1057.400923E-526.8552916
TATCAAC31400.025.5187842
ATCAACG31300.025.3752463
TCAACGC31650.025.0946354
CAACGCA31850.024.9370565
AACGCAG32650.024.18216
TATAAGA1252.3692867E-422.6246552
Produced by FastQC (version 0.11.5)