FastQCFastQC Report
Wed 25 May 2016
SRR1033026_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033026_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3445673
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC39850.035.0372244
GTATCAA43400.033.906381
ATCAACG41500.033.7574543
CAACGCA42100.033.277325
AACGCAG43450.032.126326
TATCAAC48600.029.502932
GGTATCA28300.028.9061071
GTACATG93450.027.2675911
ACGCAGA51950.026.3277857
TACATGG94350.025.4612982
CGCAGAG54400.025.0778948
ACATGGG92150.024.8957983
CATGGGG70800.020.9160124
GCAGAGT66550.019.5117939
GTATTAG16500.019.3754481
TACACCG4801.8189894E-1216.6502915
GAGTACA64050.016.442041
GTATAAG14850.015.8296151
AGAGTAC58500.015.68796210-11
GACGCGT1054.2979128E-415.68796110-11
Produced by FastQC (version 0.11.5)