##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033026_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3445673 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.640735786593794 33.0 31.0 34.0 30.0 34.0 2 31.787717522817747 33.0 31.0 34.0 30.0 34.0 3 31.994062408127526 34.0 31.0 34.0 30.0 34.0 4 35.37256698473709 37.0 35.0 37.0 33.0 37.0 5 35.47926718524944 37.0 35.0 37.0 35.0 37.0 6 35.51312994587705 37.0 36.0 37.0 35.0 37.0 7 35.41117482709473 37.0 36.0 37.0 33.0 37.0 8 35.44458078291237 37.0 36.0 37.0 35.0 37.0 9 37.110141618197666 39.0 38.0 39.0 35.0 39.0 10-11 37.109036609103654 39.0 38.0 39.0 35.0 39.0 12-13 37.05511477728734 39.0 38.0 39.0 35.0 39.0 14-15 38.475549188794176 41.0 39.0 41.0 35.0 41.0 16-17 38.48362018102124 41.0 39.0 41.0 35.0 41.0 18-19 38.47787877723742 41.0 39.0 41.0 34.0 41.0 20-21 38.49821210544356 41.0 39.0 41.0 34.0 41.0 22-23 38.462854426406686 40.5 39.0 41.0 34.0 41.0 24-25 38.46597616779073 40.5 39.0 41.0 34.0 41.0 26-27 38.37569307940713 40.0 39.0 41.0 34.0 41.0 28-29 38.388316593013904 40.0 38.5 41.0 34.0 41.0 30-31 38.535785461940236 41.0 39.0 41.0 34.0 41.0 32-33 38.523533864066614 41.0 39.0 41.0 34.0 41.0 34-35 38.48754118571321 41.0 39.0 41.0 34.0 41.0 36-37 38.41720586950648 41.0 39.0 41.0 34.0 41.0 38-39 38.33296325565426 41.0 39.0 41.0 34.0 41.0 40-41 38.26083888401482 41.0 38.0 41.0 33.5 41.0 42-43 38.14873204741134 40.0 38.0 41.0 33.0 41.0 44-45 38.013894528006574 40.0 38.0 41.0 33.0 41.0 46-47 37.88793800224224 40.0 38.0 41.0 33.0 41.0 48-49 37.77577616912574 40.0 38.0 41.0 33.0 41.0 50-51 37.650459576402056 40.0 38.0 41.0 32.5 41.0 52-53 37.498260717137114 40.0 37.0 41.0 32.0 41.0 54-55 37.34081411671973 40.0 37.0 41.0 32.0 41.0 56-57 37.16644353657471 40.0 36.5 41.0 31.5 41.0 58-59 36.97011948028731 40.0 36.0 41.0 31.0 41.0 60-61 36.744866967933405 39.0 35.5 41.0 31.0 41.0 62-63 36.48822334562798 39.0 35.0 41.0 31.0 41.0 64-65 36.20624504995105 39.0 35.0 41.0 30.5 41.0 66-67 35.89820290549916 38.0 35.0 40.0 30.0 41.0 68-69 35.57129478043912 37.0 35.0 40.0 30.0 41.0 70-71 35.21915413911883 37.0 35.0 39.5 30.0 41.0 72-73 34.860489373193566 36.5 34.0 39.0 29.0 41.0 74-75 34.49553526988777 36.0 34.0 39.0 29.0 40.5 76-77 34.12444158223952 35.5 34.0 38.5 29.0 40.0 78-79 33.73491085776276 35.0 34.0 37.0 29.0 39.0 80-81 33.38884522704272 35.0 34.0 37.0 28.0 39.0 82-83 33.17780764454433 35.0 34.0 36.5 28.0 38.5 84-85 32.96578085035928 35.0 34.0 36.0 28.5 37.0 86-87 32.80254075183571 35.0 34.0 36.0 29.0 37.0 88-89 32.59234001601429 35.0 34.0 35.0 28.0 37.0 90-91 32.391152903946484 35.0 34.0 35.0 28.0 36.0 92-93 32.189299448903014 35.0 33.5 35.0 27.0 36.0 94-95 32.002058814054614 35.0 33.0 35.0 27.0 36.0 96-97 31.84166054062588 35.0 33.0 35.0 27.0 36.0 98-99 31.677837682217667 35.0 33.0 35.0 26.5 35.0 100 31.55086858213185 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.015334484482835364 1101 2 0.07783060782327311 1101 3 0.06895575509864926 1101 4 -0.08295733489598689 1101 5 0.029863986947241017 1101 6 0.11482043118041219 1101 7 0.1216188704617025 1101 8 0.19637230980706022 1101 9 0.2124152962172161 1101 10-11 0.21171580109340482 1101 12-13 0.2818151341406363 1101 14-15 0.31648657473129305 1101 16-17 0.34173196761528857 1101 18-19 0.321605010160674 1101 20-21 0.32598620053727956 1101 22-23 0.3226340359897719 1101 24-25 0.3361633338017427 1101 26-27 0.23198886566193977 1101 28-29 0.20844184375168595 1101 30-31 0.2249615890782053 1101 32-33 0.19646130126139383 1101 34-35 0.22360062836584405 1101 36-37 0.26945805969926795 1101 38-39 0.2862178698024138 1101 40-41 0.3282163208991804 1101 42-43 0.34474093530292294 1101 44-45 0.34225855980557895 1101 46-47 0.29558041254333745 1101 48-49 0.355149880686767 1101 50-51 0.3121132192352718 1101 52-53 0.273198744203377 1101 54-55 0.2445581954176106 1101 56-57 0.27558529506672613 1101 58-59 0.31394822281689727 1101 60-61 0.3263864891118189 1101 62-63 0.3184216799620003 1101 64-65 0.2587020331590111 1101 66-67 0.22547609246471012 1101 68-69 0.23070817371530694 1101 70-71 0.17293723638655933 1101 72-73 0.25241377373637164 1101 74-75 0.16710268868290257 1101 76-77 0.17393426423873137 1101 78-79 0.1856602851103375 1101 80-81 0.16394956383131642 1101 82-83 0.2133200789660421 1101 84-85 0.23064670072865567 1101 86-87 0.3036165070105312 1101 88-89 0.27131546814763396 1101 90-91 0.37757741464682226 1101 92-93 0.34503400942558216 1101 94-95 0.36538442424504325 1101 96-97 0.3874318345276393 1101 98-99 0.4015684812115481 1101 100 0.32816698742323425 1102 1 -0.04360973081269037 1102 2 0.09287237471028931 1102 3 0.09593753973974373 1102 4 -0.07697168280927258 1102 5 0.03910690291490937 1102 6 0.06909928838793888 1102 7 0.20376266805942578 1102 8 0.17251095924379456 1102 9 0.19398759522152886 1102 10-11 0.17966185056013018 1102 12-13 0.22213628923523743 1102 14-15 0.29936959606467184 1102 16-17 0.27597199393450467 1102 18-19 0.22441343462777041 1102 20-21 0.26933885516582023 1102 22-23 0.24783122492431175 1102 24-25 0.29967489543729187 1102 26-27 0.14909477337800325 1102 28-29 0.16636964860090586 1102 30-31 0.26557099927995154 1102 32-33 0.2615527932769339 1102 34-35 0.2840686587148298 1102 36-37 0.3427192903892049 1102 38-39 0.34083760896444915 1102 40-41 0.25791387778711794 1102 42-43 0.2439991961005319 1102 44-45 0.27612696333202535 1102 46-47 0.2762704152094386 1102 48-49 0.18138320051093615 1102 50-51 0.2360646003327247 1102 52-53 0.24777162422738996 1102 54-55 0.17428027931330092 1102 56-57 0.23387827712696208 1102 58-59 0.13768369865194785 1102 60-61 0.17380882612816606 1102 62-63 0.201141688428649 1102 64-65 0.21910786243694247 1102 66-67 0.17069847276059846 1102 68-69 0.21198419322528395 1102 70-71 0.1258267689645578 1102 72-73 0.1409921642258425 1102 74-75 0.20115718363850021 1102 76-77 0.13545624135503687 1102 78-79 0.14677063820172265 1102 80-81 0.19353838109940824 1102 82-83 0.16525708974759823 1102 84-85 0.2135255215705456 1102 86-87 0.18350441858505917 1102 88-89 0.22517502456993554 1102 90-91 0.197788195309613 1102 92-93 0.18957678882416218 1102 94-95 0.2612987558488342 1102 96-97 0.214356304186623 1102 98-99 0.2980707126381503 1102 100 0.21468800615153327 1103 1 0.002178478083884272 1103 2 0.09768468247785478 1103 3 0.12798659639712895 1103 4 0.11432342432918574 1103 5 0.07485009576210899 1103 6 0.09907967475458435 1103 7 0.13415576322925205 1103 8 0.16989899576879708 1103 9 0.3238255630981257 1103 10-11 0.27422996696031277 1103 12-13 0.2935946528262363 1103 14-15 0.3493023407177489 1103 16-17 0.38338354373980366 1103 18-19 0.3871418348847371 1103 20-21 0.3659662398548633 1103 22-23 0.35955909816343024 1103 24-25 0.30142394363976877 1103 26-27 0.19966083136045398 1103 28-29 0.19893972328405596 1103 30-31 0.2596085669209174 1103 32-33 0.235673036007654 1103 34-35 0.18670987184064813 1103 36-37 0.27486541064737224 1103 38-39 0.3084370231806517 1103 40-41 0.3438068659028488 1103 42-43 0.28246823388310816 1103 44-45 0.22934326863769172 1103 46-47 0.30884770805540285 1103 48-49 0.29723577105272625 1103 50-51 0.3377415496631073 1103 52-53 0.2921314874353911 1103 54-55 0.30155253512756985 1103 56-57 0.29832435646229527 1103 58-59 0.2690022634402709 1103 60-61 0.23845522097537497 1103 62-63 0.2891959645993225 1103 64-65 0.3159953998282816 1103 66-67 0.3522058660673082 1103 68-69 0.3247329767464677 1103 70-71 0.24399145038357517 1103 72-73 0.208061585987096 1103 74-75 0.22678704075519818 1103 76-77 0.18414435865449974 1103 78-79 0.18158255011972102 1103 80-81 0.16572728253503044 1103 82-83 0.1218133815097815 1103 84-85 0.1931440733891634 1103 86-87 0.13137314567400438 1103 88-89 0.13192941812081926 1103 90-91 0.202533079919462 1103 92-93 0.17510793549553227 1103 94-95 0.12408090613217837 1103 96-97 0.17539408657943056 1103 98-99 0.19969958992372838 1103 100 0.1863731303961167 1104 1 0.015147110709889944 1104 2 0.06946755391984638 1104 3 0.13746204359654968 1104 4 0.15727623238120003 1104 5 0.11008201706167853 1104 6 0.15919707680014028 1104 7 0.2053732006297153 1104 8 0.22327652462924164 1104 9 0.29833422897992534 1104 10-11 0.28863667728521847 1104 12-13 0.2851310859761611 1104 14-15 0.31556034195958205 1104 16-17 0.26642088049622004 1104 18-19 0.36364736763535177 1104 20-21 0.29966926002322936 1104 22-23 0.2695230089139926 1104 24-25 0.2051965323830771 1104 26-27 0.182255961079953 1104 28-29 0.1712002439887783 1104 30-31 0.25949738974917125 1104 32-33 0.20248685762000918 1104 34-35 0.19246261922437213 1104 36-37 0.25627577111114164 1104 38-39 0.20394316462245854 1104 40-41 0.25635983168542964 1104 42-43 0.2555866066387438 1104 44-45 0.21166262745385467 1104 46-47 0.16520730423233232 1104 48-49 0.19838097232467078 1104 50-51 0.19670930160350508 1104 52-53 0.2043704153635062 1104 54-55 0.2485470666863847 1104 56-57 0.29445408985275634 1104 58-59 0.24068445765546187 1104 60-61 0.27143532975742346 1104 62-63 0.2611813474535154 1104 64-65 0.23039297236326206 1104 66-67 0.21739895023163314 1104 68-69 0.26816145631689636 1104 70-71 0.23607621641730248 1104 72-73 0.23759716709160017 1104 74-75 0.26454685108733855 1104 76-77 0.2140435085387935 1104 78-79 0.2446634033631554 1104 80-81 0.2962105786288518 1104 82-83 0.30233181858358904 1104 84-85 0.2996500794013315 1104 86-87 0.23923480154282117 1104 88-89 0.3314822689060719 1104 90-91 0.28004797390582326 1104 92-93 0.314058487609266 1104 94-95 0.28098101589916524 1104 96-97 0.2930714009615727 1104 98-99 0.3662701271567954 1104 100 0.37501322031231865 1105 1 0.0291503297458533 1105 2 0.0958533340492096 1105 3 0.11735878854138093 1105 4 0.11865856943761344 1105 5 0.11711137333288235 1105 6 0.1470895076921579 1105 7 0.10334680057393086 1105 8 0.14440915328443538 1105 9 0.15413470321216494 1105 10-11 0.1960297326339031 1105 12-13 0.14962225590718248 1105 14-15 0.27447961314211256 1105 16-17 0.20725481793173373 1105 18-19 0.2669042382059317 1105 20-21 0.2692980352836045 1105 22-23 0.24589094447716064 1105 24-25 0.15510551478070056 1105 26-27 0.21926858265508997 1105 28-29 0.23897479347020578 1105 30-31 0.2314265255729353 1105 32-33 0.21086586135272256 1105 34-35 0.2052857863811326 1105 36-37 0.26161055373957254 1105 38-39 0.2936018362358226 1105 40-41 0.2917946841655308 1105 42-43 0.31306548094162423 1105 44-45 0.2623762244356769 1105 46-47 0.318280613010991 1105 48-49 0.2825816409698447 1105 50-51 0.2430409332735053 1105 52-53 0.2884113906134047 1105 54-55 0.20258709710260803 1105 56-57 0.2626279656554047 1105 58-59 0.2602752332731413 1105 60-61 0.2664401096256128 1105 62-63 0.21157537197875342 1105 64-65 0.26591399450001063 1105 66-67 0.21684527357022176 1105 68-69 0.22583575440655324 1105 70-71 0.2121089423202278 1105 72-73 0.22575233601259015 1105 74-75 0.20570945861373957 1105 76-77 0.19939712182620184 1105 78-79 0.2038879507197393 1105 80-81 0.23400732963877147 1105 82-83 0.16881862990194918 1105 84-85 0.2229553922960008 1105 86-87 0.22225838896490302 1105 88-89 0.22128877191984486 1105 90-91 0.21180249291327868 1105 92-93 0.26412831865732755 1105 94-95 0.2806857450599125 1105 96-97 0.29159275688454755 1105 98-99 0.3529099107609994 1105 100 0.31616258366357997 1106 1 -0.0633024948712766 1106 2 -0.018378468325529695 1106 3 0.05120015562594915 1106 4 0.035197981589675464 1106 5 0.03216349732014834 1106 6 0.006362714948863868 1106 7 0.06034950643439885 1106 8 0.09564007864146618 1106 9 0.08106643068112618 1106 10-11 0.08810143350688548 1106 12-13 0.11629637049825448 1106 14-15 0.13100355198513824 1106 16-17 0.1698282103366182 1106 18-19 0.12651447224465073 1106 20-21 0.17829037048350926 1106 22-23 0.12110353022261933 1106 24-25 0.045570137303819536 1106 26-27 0.10319366506620753 1106 28-29 0.12387739135218112 1106 30-31 0.16241281122083961 1106 32-33 0.11275956995645231 1106 34-35 0.1639150598738368 1106 36-37 0.09814429628221433 1106 38-39 0.11330431954443299 1106 40-41 0.15229679894952852 1106 42-43 0.11931878452263334 1106 44-45 0.09427525814130178 1106 46-47 0.044341819200703014 1106 48-49 0.03674314412504032 1106 50-51 0.08635389472901522 1106 52-53 0.0772563356546172 1106 54-55 0.12130812771160038 1106 56-57 0.10701510154786575 1106 58-59 0.01749862080571063 1106 60-61 0.08738596977721613 1106 62-63 -3.4970649513610397E-4 1106 64-65 -0.012078373182035307 1106 66-67 0.04051008547403967 1106 68-69 0.07594604602724786 1106 70-71 0.052018661664718024 1106 72-73 0.06791011454938456 1106 74-75 0.046787649024032874 1106 76-77 0.051959125796905425 1106 78-79 0.031120088743229246 1106 80-81 0.10150688423976817 1106 82-83 0.06599819607840374 1106 84-85 0.032335938251826235 1106 86-87 0.10538815116613165 1106 88-89 0.04133842037453661 1106 90-91 0.0021683894251935953 1106 92-93 0.026160834261055754 1106 94-95 0.0966189224133629 1106 96-97 0.0921810772922882 1106 98-99 0.07045331624733109 1106 100 0.10313291566055227 1107 1 0.02561301765502222 1107 2 0.09277776266736737 1107 3 0.13639956620667704 1107 4 0.17103447613762057 1107 5 0.14266357088380488 1107 6 0.20751599581185332 1107 7 0.23074844135408057 1107 8 0.20175708579913731 1107 9 0.31776399913703557 1107 10-11 0.28034917085583544 1107 12-13 0.24858087829676379 1107 14-15 0.3095040062596439 1107 16-17 0.28410247837829417 1107 18-19 0.3540203004575986 1107 20-21 0.38840781826469595 1107 22-23 0.33879043119078034 1107 24-25 0.27966237702120367 1107 26-27 0.19093813148705152 1107 28-29 0.21690183192716006 1107 30-31 0.24530816275650835 1107 32-33 0.22775210741703233 1107 34-35 0.23085271126706886 1107 36-37 0.33836977266373225 1107 38-39 0.38356490550545175 1107 40-41 0.3425461076787357 1107 42-43 0.3012792141040137 1107 44-45 0.327557103239009 1107 46-47 0.35978832780917 1107 48-49 0.35576380337669633 1107 50-51 0.39913425186917095 1107 52-53 0.3947448422821722 1107 54-55 0.4217995063550859 1107 56-57 0.3982436031397256 1107 58-59 0.3925152105465415 1107 60-61 0.3101160950781079 1107 62-63 0.37251251106215477 1107 64-65 0.4398112077781775 1107 66-67 0.4632865293313202 1107 68-69 0.4417880161376502 1107 70-71 0.48379986516604845 1107 72-73 0.41405873511717317 1107 74-75 0.47260831980318585 1107 76-77 0.4914521509010612 1107 78-79 0.45106253087442383 1107 80-81 0.4750101293826745 1107 82-83 0.4445919700150256 1107 84-85 0.4163622550790933 1107 86-87 0.421330702505216 1107 88-89 0.4301666613842201 1107 90-91 0.41913901174334 1107 92-93 0.44712700559914254 1107 94-95 0.429456807086801 1107 96-97 0.4803240008482774 1107 98-99 0.47571582851426086 1107 100 0.4825902282666341 1108 1 -0.03234843077174432 1108 2 0.03131025923182307 1108 3 0.06774356436857687 1108 4 0.02756521796806055 1108 5 0.10675110628855577 1108 6 0.1279013663087909 1108 7 0.10288904693338452 1108 8 0.10680044292105606 1108 9 0.18672079073615322 1108 10-11 0.12589320012715888 1108 12-13 0.13659967001381546 1108 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-0.1228387999461944 2214 84-85 -0.11006047016608278 2214 86-87 -0.10240484892986501 2214 88-89 -0.10877141673017832 2214 90-91 -0.13461906764551657 2214 92-93 -0.12609465422250565 2214 94-95 -0.09188653929517443 2214 96-97 -0.03093683674678971 2214 98-99 -0.15559985459002235 2214 100 -0.1380350942529489 2215 1 -0.1299270288521619 2215 2 -0.19883814192862204 2215 3 -0.25987393763017863 2215 4 -0.24237547370081813 2215 5 -0.23158532565226153 2215 6 -0.2540348522610216 2215 7 -0.2600124849371994 2215 8 -0.32226539193909076 2215 9 -0.33640409648599956 2215 10-11 -0.3202241848991818 2215 12-13 -0.3988145141531163 2215 14-15 -0.43646181465226164 2215 16-17 -0.4854360809550329 2215 18-19 -0.47433981659012403 2215 20-21 -0.44345233031580733 2215 22-23 -0.42986499644381126 2215 24-25 -0.42598044434608795 2215 26-27 -0.4574822137797625 2215 28-29 -0.4507145814709972 2215 30-31 -0.4715368005909184 2215 32-33 -0.48009409744189213 2215 34-35 -0.460676563740563 2215 36-37 -0.47831793011464896 2215 38-39 -0.5107013627808072 2215 40-41 -0.47125281671128505 2215 42-43 -0.406851045484089 2215 44-45 -0.3278769326316038 2215 46-47 -0.4263542563289633 2215 48-49 -0.36774986913852814 2215 50-51 -0.36977631016961965 2215 52-53 -0.4308102629603141 2215 54-55 -0.46332476151611246 2215 56-57 -0.47489020327492426 2215 58-59 -0.46943142584702713 2215 60-61 -0.5630823657556121 2215 62-63 -0.6097358176394465 2215 64-65 -0.6855094518631191 2215 66-67 -0.6360056086668706 2215 68-69 -0.5952037836287118 2215 70-71 -0.5659309023442418 2215 72-73 -0.6728541746470214 2215 74-75 -0.6128799011149795 2215 76-77 -0.5669494586395345 2215 78-79 -0.5948360720523524 2215 80-81 -0.6120512319387359 2215 82-83 -0.5533935070624807 2215 84-85 -0.6215312385597116 2215 86-87 -0.49721922781712635 2215 88-89 -0.5248405697664467 2215 90-91 -0.5370375905190556 2215 92-93 -0.5564596035784604 2215 94-95 -0.667447013170495 2215 96-97 -0.6385128533194617 2215 98-99 -0.5760890900370583 2215 100 -0.5522380317521751 2216 1 -0.17131999393575725 2216 2 -0.3420723879867289 2216 3 -0.3233357299298092 2216 4 -0.42311084293426404 2216 5 -0.37100719370053525 2216 6 -0.4116319860386497 2216 7 -0.42156622333700255 2216 8 -0.3134415811547129 2216 9 -0.376252323104147 2216 10-11 -0.3612754140465668 2216 12-13 -0.4524922806580989 2216 14-15 -0.5449812963858562 2216 16-17 -0.5799250985405067 2216 18-19 -0.5974722757156243 2216 20-21 -0.46632337719272243 2216 22-23 -0.4402164588968205 2216 24-25 -0.4990158078395339 2216 26-27 -0.5401886558291196 2216 28-29 -0.483872001124908 2216 30-31 -0.6803240400596522 2216 32-33 -0.7215588246501596 2216 34-35 -0.8739757347826611 2216 36-37 -0.8573920897191343 2216 38-39 -0.7629643400879473 2216 40-41 -0.6798375939859156 2216 42-43 -0.7296470363959955 2216 44-45 -0.5836671891127807 2216 46-47 -0.5642956763471361 2216 48-49 -0.6565563703074773 2216 50-51 -0.5797874293027405 2216 52-53 -0.5752564121824264 2216 54-55 -0.6866858519128982 2216 56-57 -0.7517872555057821 2216 58-59 -0.6991468307750566 2216 60-61 -0.7038702087721518 2216 62-63 -0.6919072043874053 2216 64-65 -0.709609282835828 2216 66-67 -0.7137316793405759 2216 68-69 -0.6630937446572531 2216 70-71 -0.6510605815653676 2216 72-73 -0.6629027563702223 2216 74-75 -0.6994962043168584 2216 76-77 -0.48444490962803144 2216 78-79 -0.5131623579397697 2216 80-81 -0.48712060239623867 2216 82-83 -0.6657933991407674 2216 84-85 -0.5673842330117793 2216 86-87 -0.5857169920018848 2216 88-89 -0.673895939949297 2216 90-91 -0.6403205684607371 2216 92-93 -0.8302284883640425 2216 94-95 -0.7396923878072954 2216 96-97 -0.8130590238788749 2216 98-99 -0.7574962694949292 2216 100 -0.714913032157849 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28370.0 3 6015.0 4 3228.0 5 2867.0 6 2512.0 7 2736.0 8 3386.0 9 3806.0 10 4150.0 11 4668.0 12 5273.0 13 5858.0 14 6782.0 15 7114.0 16 7189.0 17 7288.0 18 6867.0 19 6628.0 20 6638.0 21 6826.0 22 8002.0 23 11014.0 24 17572.0 25 22673.0 26 22733.0 27 20278.0 28 23400.0 29 29235.0 30 36034.0 31 44526.0 32 58662.0 33 79321.0 34 116138.0 35 182110.0 36 335990.0 37 713959.0 38 1135741.0 39 459348.0 40 736.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14393683098467 19.55190909943614 13.260068643539425 24.044085426039764 2 15.51313861789161 20.04709845964636 40.291161504572514 24.148601417889516 3 18.214166347976253 25.36163013850184 29.898184582585248 26.52601893093667 4 12.233354786544421 16.709718304623205 39.08601769541397 31.970909213418402 5 13.062193604757727 37.860278105286824 35.818945447014265 13.258582842941186 6 31.078818552458365 38.1591498745749 17.897389600928577 12.86464197203816 7 27.432851229166648 32.3268896888617 22.679810156052792 17.56044892591886 8 25.17835048641306 36.442530573850334 20.42252150417214 17.95659743556447 9 25.054431611962958 17.007035512795895 20.878702693155603 37.05983018208555 10-11 23.919584340429488 26.88332306869945 29.01918518174212 20.177907409128938 12-13 24.656077062560747 24.163686763827698 28.833630415737666 22.346605757873895 14-15 21.907346704817783 26.08836870271965 25.85134776695977 26.1529368255028 16-17 21.821516007813273 29.37932230272264 26.367079466256648 22.43208222320744 18-19 21.766664425004116 28.540774994549565 28.067716413516152 21.62484416693017 20-21 22.98376490727068 27.827786392941213 27.520792221284456 21.667656478503655 22-23 22.475020954710097 27.598148116152327 27.502002548685127 22.424828380452453 24-25 22.41975584586736 27.67560098753104 27.588647747212953 22.315995419388642 26-27 22.179251863160722 27.642144051477985 27.61045294728723 22.56815113807407 28-29 22.16745106170671 27.74401771727975 27.673724189163206 22.41480703185033 30-31 22.518183224165007 28.176304483416363 27.22597734149173 22.079534950926895 32-33 22.116287188352054 27.78165504358221 27.756416511866465 22.34564125619927 34-35 22.200278726103853 27.517990780151518 27.86657257749657 22.41515791624806 36-37 22.564680401483734 27.861576296820257 27.226245548075127 22.347497753620885 38-39 22.424989729394785 28.07308924266467 27.38132459076957 22.12059643717098 40-41 22.228705809363465 27.686038569727135 27.732278906491555 22.35297671441785 42-43 22.11372047762647 27.635517783491604 27.666129652009275 22.58463208687265 44-45 22.264831146245726 27.741173439893075 27.625975418516752 22.368019995344447 46-47 22.307947789745665 27.87496875874519 27.532385980856315 22.284697470652826 48-49 22.274688326796774 27.705264154557437 27.533469771667402 22.486577746978387 50-51 22.277822374069046 27.720246303186887 27.552439042807404 22.449492279936663 52-53 22.357302150631167 27.574423728811098 27.54169165016872 22.526582470389016 54-55 22.41537332963933 27.978987738346028 27.39374075409956 22.21189817791508 56-57 22.130850816512545 27.785313272734115 27.53754464968322 22.546291261070124 58-59 22.123586821119094 27.396419881493284 27.792724906695597 22.687268390692022 60-61 22.281778451118907 27.858767957074214 27.441854994788045 22.417598597018838 62-63 22.254694084754757 27.777571258926333 27.413838318391853 22.553896337927053 64-65 22.20586578258642 27.537819870199115 27.562461012688516 22.693853334525944 66-67 22.04153761487606 27.678272978479022 27.45789390381848 22.822295502826435 68-69 22.463322081328947 27.718555555700764 27.464300042995465 22.353822319974828 70-71 22.349310097044214 27.583224884507988 27.49153402224654 22.575930996201254 72-73 22.226849034707048 27.685026158671693 27.615101277101882 22.473023529519377 74-75 22.241907496280454 27.750839991874866 27.433617011329165 22.573635500515515 76-77 22.307609215174644 27.762386179444903 27.268295062602395 22.66170954277806 78-79 22.424537190958546 27.85483962292914 27.364467473790725 22.356155712321588 80-81 22.45259092199179 27.503297312717358 27.593364580074432 22.450747185216425 82-83 22.230962348927928 27.62569666940195 27.702542999390804 22.44079798227932 84-85 22.199622411701895 27.614537657573436 27.461963531735346 22.723876398989322 86-87 22.37963329644155 27.73712455448235 27.402441941893695 22.4808002071824 88-89 22.16189394873108 27.598043848352518 27.4627879269319 22.777274275984503 90-91 22.155199407028817 27.573377788752275 27.56415979272719 22.707263011491722 92-93 22.38352545908105 27.716945699991175 27.369252486447067 22.530276354480712 94-95 22.195096918723916 27.47352258591614 27.484671429216384 22.84670906614356 96-97 22.194226593579298 27.455102994507996 27.58832741007062 22.76234300184209 98-99 22.11712416645883 27.659267444807796 27.471022948495015 22.75258544023836 100 22.325983453114787 27.415662019140445 27.335462501789394 22.922892025955374 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 347.0 1 326.5 2 295.5 3 293.5 4 328.0 5 365.0 6 363.0 7 331.5 8 298.5 9 274.0 10 249.0 11 242.5 12 235.5 13 227.0 14 263.5 15 318.5 16 411.0 17 541.5 18 738.0 19 1067.0 20 1533.0 21 2220.0 22 3374.0 23 5140.0 24 7617.0 25 10993.5 26 15576.0 27 21513.5 28 29361.0 29 38201.5 30 47189.0 31 57424.0 32 67571.0 33 78355.5 34 89405.0 35 98055.0 36 104814.0 37 111529.0 38 117684.5 39 121070.5 40 123769.5 41 126756.0 42 127137.0 43 126351.5 44 124996.5 45 123219.0 46 120578.5 47 116827.5 48 115464.5 49 114222.5 50 110736.5 51 106728.0 52 101305.5 53 97621.0 54 95813.0 55 91476.0 56 84232.0 57 76940.5 58 69149.5 59 60371.5 60 52593.0 61 45314.0 62 39275.5 63 32730.0 64 26578.0 65 22071.0 66 17494.0 67 13630.0 68 11043.5 69 9020.5 70 7178.0 71 5232.0 72 3965.5 73 3109.5 74 2293.5 75 1553.5 76 932.5 77 628.5 78 442.0 79 286.5 80 202.0 81 130.5 82 76.5 83 49.0 84 36.0 85 28.0 86 19.5 87 17.5 88 14.5 89 5.5 90 5.5 91 6.0 92 6.0 93 4.0 94 3.0 95 4.5 96 3.5 97 2.5 98 5.0 99 5.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0605396971796221 2 0.04060164734146276 3 0.05490944729810403 4 0.0731642265531291 5 0.08764615794940495 6 0.08651430359178018 7 0.08869094658721242 8 0.09966122728419093 9 0.09519185366690339 10-11 0.09963220537758516 12-13 0.10408706804156981 14-15 0.10366625039578625 16-17 0.07422352614423947 18-19 0.09540951796644662 20-21 0.08995339952456312 22-23 0.05620092214206049 24-25 0.020837728942938 26-27 0.1805743029010588 28-29 0.06544439939599608 30-31 0.07532635859525846 32-33 0.07328031417955215 34-35 0.020721641316514945 36-37 0.0521668771238594 38-39 0.0752973366886527 40-41 0.08101465228998805 42-43 0.07441216853717691 44-45 0.07172764217614382 46-47 0.07863485594831547 48-49 0.07555853384810456 50-51 0.070726386398245 52-53 0.07451374521029709 54-55 0.06544439939599608 56-57 0.06789675050418308 58-59 0.06499455984360675 60-61 0.06235356634248229 62-63 0.06820148052354358 64-65 0.06515418032993844 66-67 0.06640212231398628 68-69 0.06635858945407763 70-71 0.05910311280263682 72-73 0.05739082031289679 74-75 0.05916115661584834 76-77 0.054416074885806055 78-79 0.051020511812931754 80-81 0.045869123390408786 82-83 0.051862147104498886 84-85 0.041951165998630746 86-87 0.03961490251686681 88-89 0.04241551650432296 90-91 0.03841049339272763 92-93 0.040659691154674285 94-95 0.039745501096592736 96-97 0.04308302035625551 98-99 0.0430104655897411 100 0.0511365994393548 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3445673.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.822394359611444 #Duplication Level Percentage of deduplicated Percentage of total 1 76.23876979639404 47.132632918443065 2 13.923208603478507 17.215321860707665 3 4.531437493821232 8.404329472768367 4 1.9223660477638689 4.7538108763354225 5 1.015235421610549 3.1382142301326876 6 0.5861783666089639 2.1743370087343354 7 0.37977892055917284 1.6435189537393713 8 0.2607285569792834 1.2895090936308546 9 0.19157176355602343 1.0659082603254049 >10 0.8907290575830658 9.470616200263617 >50 0.04347350210114584 1.7988899840174037 >100 0.015957895836225432 1.6754523685762073 >500 5.645737080222295E-4 0.23745877232566157 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.902190660576323E-5 0.0 12-13 0.0 0.0 0.0 2.902190660576323E-5 0.0 14-15 0.0 0.0 0.0 8.706571981728968E-5 0.0 16-17 0.0 0.0 0.0 1.1608762642305292E-4 0.0 18-19 0.0 0.0 0.0 1.1608762642305292E-4 0.0 20-21 0.0 0.0 0.0 1.4510953302881614E-4 0.0 22-23 0.0 0.0 0.0 1.5962048633169775E-4 0.0 24-25 0.0 0.0 0.0 1.7413143963457937E-4 0.0 26-27 0.0 0.0 0.0 2.321752528461058E-4 0.0 28-29 0.0 0.0 0.0 8.706571981728969E-4 0.0 30-31 0.0 0.0 0.0 0.003990512158292444 0.0 32-33 0.0 0.0 0.0 0.011129901183310198 0.0 34-35 0.0 0.0 0.0 0.02175191900101954 0.0 36-37 0.0 0.0 0.0 0.03997767634943884 0.0 38-39 0.0 0.0 0.0 0.07313520464652334 0.0 40-41 0.0 0.0 0.0 0.11543463352442324 0.0 42-43 0.0 0.0 0.0 0.15695047092396752 0.0 44-45 0.0 0.0 0.0 0.20457541966402498 0.0 46-47 0.0 0.0 0.0 0.26485391968419525 0.0 48-49 0.0 0.0 0.0 0.33105288865194116 0.0 50-51 0.0 0.0 0.0 0.40489913001030564 0.0 52-53 0.0 0.0 0.0 0.4780633565634348 0.0 54-55 0.0 0.0 0.0 0.5597310017520525 0.0 56-57 0.0 0.0 0.0 0.6565045493289701 0.0 58-59 0.0 0.0 0.0 0.7683259554809757 0.0 60-61 0.0 0.0 0.0 0.8862854948801004 0.0 62-63 0.0 0.0 0.0 1.0082210354842145 0.0 64-65 0.0 0.0 0.0 1.1377022718058272 0.0 66-67 0.0 0.0 0.0 1.2878325946774405 0.0 68-69 0.0 0.0 0.0 1.4558839448781122 0.0 70-71 0.0 0.0 0.0 1.6435831258508862 0.0 72-73 0.0 0.0 0.0 1.8402355650115378 0.0 74-75 0.0 0.0 0.0 2.038164968062843 0.0 76-77 0.0 0.0 0.0 2.254610347528625 0.0 78-79 0.0 0.0 0.0 2.504692116750487 0.0 80-81 0.0 0.0 0.0 2.781154799076987 0.0 82-83 0.0 0.0 0.0 3.0711562008350763 0.0 84-85 0.0 0.0 0.0 3.3626232088767565 0.0 86-87 0.0 0.0 0.0 3.6686156811746207 0.0 88 0.0 0.0 0.0 3.9238778607256117 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAACGC 3985 0.0 35.037224 4 GTATCAA 4340 0.0 33.90638 1 ATCAACG 4150 0.0 33.757454 3 CAACGCA 4210 0.0 33.27732 5 AACGCAG 4345 0.0 32.12632 6 TATCAAC 4860 0.0 29.50293 2 GGTATCA 2830 0.0 28.906107 1 GTACATG 9345 0.0 27.267591 1 ACGCAGA 5195 0.0 26.327785 7 TACATGG 9435 0.0 25.461298 2 CGCAGAG 5440 0.0 25.077894 8 ACATGGG 9215 0.0 24.895798 3 CATGGGG 7080 0.0 20.916012 4 GCAGAGT 6655 0.0 19.511793 9 GTATTAG 1650 0.0 19.375448 1 TACACCG 480 1.8189894E-12 16.650291 5 GAGTACA 6405 0.0 16.44204 1 GTATAAG 1485 0.0 15.829615 1 AGAGTAC 5850 0.0 15.687962 10-11 GACGCGT 105 4.2979128E-4 15.687961 10-11 >>END_MODULE