FastQCFastQC Report
Wed 25 May 2016
SRR1033026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033026_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3445673
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50100.14539975209487377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41590.12070210957336927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47200.046.8158721
GTATCAA81100.042.5838971
ATCAACG95550.035.7125363
TCAACGC96050.035.427224
CAACGCA97900.034.661745
TATCAAC100700.033.9098242
AACGCAG100600.033.82446
ACGCAGA116000.029.0097897
CGCAGAG119950.028.0153058
GTACATG92900.026.676711
TACATGG94700.025.5143412
GCAGAGT133050.024.6917489
ACATGGG94150.024.3121513
GAGTACT91250.020.42118512-13
AGAGTAC126200.019.9614410-11
CATGGGG71700.019.8618344
GTACGAC2652.0971129E-819.5101933
CAGAGTA135900.019.31479510-11
CGTATAC2451.9649633E-719.184423
GTACTTT101400.018.28435914-15
Produced by FastQC (version 0.11.5)