##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033026_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3445673 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.72699788981717 34.0 31.0 34.0 31.0 34.0 2 32.726383786273395 34.0 31.0 34.0 31.0 34.0 3 32.8271034424915 34.0 31.0 34.0 31.0 34.0 4 36.31189494766335 37.0 37.0 37.0 35.0 37.0 5 36.294723265962844 37.0 37.0 37.0 35.0 37.0 6 36.35953092472791 37.0 37.0 37.0 35.0 37.0 7 36.31045517087663 37.0 37.0 37.0 35.0 37.0 8 36.31840369065782 37.0 37.0 37.0 35.0 37.0 9 38.12771554352372 39.0 39.0 39.0 37.0 39.0 10-11 38.08680669930084 39.0 39.0 39.0 36.0 39.0 12-13 38.06386110928112 39.0 39.0 39.0 35.0 39.0 14-15 39.56869078986892 41.0 40.0 41.0 37.0 41.0 16-17 39.56519147347993 41.0 40.0 41.0 37.0 41.0 18-19 39.57950348161303 41.0 40.0 41.0 37.0 41.0 20-21 39.56774148330384 41.0 40.0 41.0 37.0 41.0 22-23 39.49669875812359 41.0 39.0 41.0 36.5 41.0 24-25 39.44021284085866 41.0 39.0 41.0 36.0 41.0 26-27 39.332214635573365 41.0 39.0 41.0 36.0 41.0 28-29 39.28082104715102 41.0 39.0 41.0 36.0 41.0 30-31 39.28186148250283 41.0 39.0 41.0 36.0 41.0 32-33 39.24588897437453 41.0 39.0 41.0 35.5 41.0 34-35 39.16523288774066 41.0 39.0 41.0 35.0 41.0 36-37 39.082515375080575 41.0 39.0 41.0 35.0 41.0 38-39 38.991816112556236 41.0 39.0 41.0 35.0 41.0 40-41 38.8918761298591 41.0 39.0 41.0 35.0 41.0 42-43 38.779117896561864 41.0 39.0 41.0 35.0 41.0 44-45 38.62032555033516 41.0 38.5 41.0 35.0 41.0 46-47 38.42885816500869 41.0 38.0 41.0 35.0 41.0 48-49 38.23146015306734 40.0 38.0 41.0 34.0 41.0 50-51 38.0926212382893 40.0 38.0 41.0 34.0 41.0 52-53 37.95629649128051 40.0 38.0 41.0 34.0 41.0 54-55 37.803830050036666 40.0 37.0 41.0 33.5 41.0 56-57 37.64792030468358 40.0 37.0 41.0 33.0 41.0 58-59 37.46316800810756 40.0 37.0 41.0 33.0 41.0 60-61 37.26433878084194 40.0 36.0 41.0 33.0 41.0 62-63 37.028530855945995 39.0 35.5 41.0 33.0 41.0 64-65 36.7577506629329 39.0 35.0 41.0 32.0 41.0 66-67 36.47049038025372 39.0 35.0 41.0 32.0 41.0 68-69 36.1580625903851 37.5 35.0 40.0 31.5 41.0 70-71 35.827134060602965 37.0 35.0 40.0 31.0 41.0 72-73 35.475207600953425 37.0 35.0 39.0 31.0 41.0 74-75 35.122867869353826 36.0 35.0 39.0 31.0 41.0 76-77 34.769172089168066 36.0 35.0 38.5 31.0 40.0 78-79 34.40897453124542 35.0 34.5 37.0 30.5 39.0 80-81 34.0862666016189 35.0 34.0 37.0 30.0 39.0 82-83 33.79213349612688 35.0 34.0 36.5 30.0 39.0 84-85 33.576105161459026 35.0 34.0 36.0 30.0 37.0 86-87 33.37504327891823 35.0 34.0 36.0 30.0 37.0 88-89 33.17269920854359 35.0 34.0 35.5 30.0 37.0 90-91 32.99426106307824 35.0 34.0 35.0 30.0 36.0 92-93 32.828153455072496 35.0 34.0 35.0 29.5 36.0 94-95 32.679531836015784 35.0 34.0 35.0 29.0 36.0 96-97 32.54066767217899 35.0 34.0 35.0 29.0 36.0 98-99 32.42096855969792 35.0 34.0 35.0 29.0 35.0 100 32.320077093792705 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1494136455714994 1101 2 0.1523791919291142 1101 3 0.15121303248299967 1101 4 0.14169966811050472 1101 5 0.09865734491145162 1101 6 0.14226907900496144 1101 7 0.18106828794581986 1101 8 0.1435982826354234 1101 9 0.21200269967879137 1101 10-11 0.2052470567187612 1101 12-13 0.22061800884562643 1101 14-15 0.31203115964117245 1101 16-17 0.3085917778777798 1101 18-19 0.2691526119212426 1101 20-21 0.2930666765138028 1101 22-23 0.289453879281254 1101 24-25 0.3391986252162411 1101 26-27 0.33838048195197956 1101 28-29 0.298196577558123 1101 30-31 0.33489388187356894 1101 32-33 0.3400518639910288 1101 34-35 0.3570296753658937 1101 36-37 0.34308221821608953 1101 38-39 0.29268096590590886 1101 40-41 0.2770044819176931 1101 42-43 0.3274572772034361 1101 44-45 0.313030199837776 1101 46-47 0.2792677456421373 1101 48-49 0.26054929411096595 1101 50-51 0.31511572384783904 1101 52-53 0.3218941347536344 1101 54-55 0.3303603135896722 1101 56-57 0.29324994170217167 1101 58-59 0.3017750469702918 1101 60-61 0.29478076154966004 1101 62-63 0.31178479757536337 1101 64-65 0.3150201128556134 1101 66-67 0.36508157093606286 1101 68-69 0.36322201948591726 1101 70-71 0.3163406634267645 1101 72-73 0.26037419846751675 1101 74-75 0.21524288939397707 1101 76-77 0.26409685770585867 1101 78-79 0.286919411894182 1101 80-81 0.3029231686941003 1101 82-83 0.2994811602951586 1101 84-85 0.3097743142817748 1101 86-87 0.36800108049715163 1101 88-89 0.3282999583908861 1101 90-91 0.34138606661908 1101 92-93 0.36617191872099397 1101 94-95 0.3302119397715799 1101 96-97 0.2981932885559928 1101 98-99 0.34128206892351187 1101 100 0.3257935102057843 1102 1 0.19434171625871954 1102 2 0.11232142213516738 1102 3 0.1366824702380427 1102 4 0.10319014154065798 1102 5 0.12252627567199426 1102 6 0.11700770945740402 1102 7 0.15341022417540273 1102 8 0.1558689804606601 1102 9 0.166904243538049 1102 10-11 0.1873499453919294 1102 12-13 0.21525238792752077 1102 14-15 0.31676813114088986 1102 16-17 0.3123082713538352 1102 18-19 0.2778840094170363 1102 20-21 0.26286618077855906 1102 22-23 0.2931913269332256 1102 24-25 0.346900347060469 1102 26-27 0.33896444683162485 1102 28-29 0.33657847195275536 1102 30-31 0.3361065444531022 1102 32-33 0.35425696056805833 1102 34-35 0.35070081415582877 1102 36-37 0.269641476867001 1102 38-39 0.28940366519774585 1102 40-41 0.3309820286974059 1102 42-43 0.31782064965155854 1102 44-45 0.2739759612570083 1102 46-47 0.26487903447748806 1102 48-49 0.2536332090192417 1102 50-51 0.267427230172089 1102 52-53 0.29207974626456235 1102 54-55 0.26181159981396007 1102 56-57 0.24082237628095982 1102 58-59 0.27427998158215416 1102 60-61 0.2757099991044427 1102 62-63 0.24409040100203327 1102 64-65 0.2512398501250104 1102 66-67 0.3047607509151433 1102 68-69 0.25206950697883457 1102 70-71 0.2486337860827561 1102 72-73 0.26800965393742615 1102 74-75 0.25510960924755466 1102 76-77 0.25451273629755633 1102 78-79 0.2879166665108883 1102 80-81 0.2269822899841074 1102 82-83 0.3079307144685046 1102 84-85 0.292499551636098 1102 86-87 0.29539910970379424 1102 88-89 0.3064374865378028 1102 90-91 0.2769998467804271 1102 92-93 0.3172757596021256 1102 94-95 0.3300812175796324 1102 96-97 0.3745278788501949 1102 98-99 0.3299174702215737 1102 100 0.3475631125718124 1103 1 0.12714062049563069 1103 2 0.11999111091327563 1103 3 0.1297419078548856 1103 4 0.08217411570202415 1103 5 0.1076401251793726 1103 6 0.12525328809130798 1103 7 0.14613804008300946 1103 8 0.1270236387020276 1103 9 0.15403150071388438 1103 10-11 0.15734962862033086 1103 12-13 0.13882804326200215 1103 14-15 0.21478820287471478 1103 16-17 0.23827605802649998 1103 18-19 0.23808903650422053 1103 20-21 0.244626320124965 1103 22-23 0.23114395344106953 1103 24-25 0.22153384714255964 1103 26-27 0.21072268141060135 1103 28-29 0.2463171390578438 1103 30-31 0.22776559871654456 1103 32-33 0.18831443194815733 1103 34-35 0.2334637815968108 1103 36-37 0.2495985927043094 1103 38-39 0.2462830731906962 1103 40-41 0.2596571091672786 1103 42-43 0.25571472628463 1103 44-45 0.24618968949464914 1103 46-47 0.26477372745890904 1103 48-49 0.23214152004408106 1103 50-51 0.25236021182917057 1103 52-53 0.23085184114138713 1103 54-55 0.24562178934958467 1103 56-57 0.28557330759637267 1103 58-59 0.29827979823162565 1103 60-61 0.29472293878768596 1103 62-63 0.3002855915263609 1103 64-65 0.28064623163378855 1103 66-67 0.22899633942781605 1103 68-69 0.20283807350733696 1103 70-71 0.2733017195599885 1103 72-73 0.2821372996527103 1103 74-75 0.25732167730775046 1103 76-77 0.31448854303874185 1103 78-79 0.31991344895733675 1103 80-81 0.27106046094026937 1103 82-83 0.251424246419937 1103 84-85 0.3434984465215791 1103 86-87 0.276534556138202 1103 88-89 0.2609980252463089 1103 90-91 0.24112208347115427 1103 92-93 0.2959326176590693 1103 94-95 0.2659117272090512 1103 96-97 0.2334840580274289 1103 98-99 0.26181509258173463 1103 100 0.23894594567497052 1104 1 0.07404456001385284 1104 2 0.09180039593818634 1104 3 0.12462902406007004 1104 4 0.0870913643479696 1104 5 0.048978220699794406 1104 6 0.07231461763668534 1104 7 0.0843104786050688 1104 8 0.1072042395014563 1104 9 0.10576432343700048 1104 10-11 0.1851822103380556 1104 12-13 0.17687925435315321 1104 14-15 0.2364596526556042 1104 16-17 0.18494602995650666 1104 18-19 0.18624157446534184 1104 20-21 0.22014044851034953 1104 22-23 0.20835239310908804 1104 24-25 0.19008088004148505 1104 26-27 0.18353304122821612 1104 28-29 0.21778667118640982 1104 30-31 0.2085835527230273 1104 32-33 0.2285381056610376 1104 34-35 0.1701530210349418 1104 36-37 0.17806744489049464 1104 38-39 0.1887532199480546 1104 40-41 0.21833495784600387 1104 42-43 0.20272519331082606 1104 44-45 0.21033811863740226 1104 46-47 0.13408169857179786 1104 48-49 0.09465257773039326 1104 50-51 0.14834201605764008 1104 52-53 0.24445915856830425 1104 54-55 0.1983456889968238 1104 56-57 0.20225318833097106 1104 58-59 0.14945386234599312 1104 60-61 0.12758808954463063 1104 62-63 0.12235433215554536 1104 64-65 0.1088606053205936 1104 66-67 0.18925162474210566 1104 68-69 0.2584275980462536 1104 70-71 0.21234739986516615 1104 72-73 0.16135986015313364 1104 74-75 0.15748355109518286 1104 76-77 0.18153422351856818 1104 78-79 0.1475369224159664 1104 80-81 0.17099295131503567 1104 82-83 0.14324281156250862 1104 84-85 0.1649836652622838 1104 86-87 0.20597269949995223 1104 88-89 0.23965711635186437 1104 90-91 0.2276900998442528 1104 92-93 0.2509775290452012 1104 94-95 0.25128165838791716 1104 96-97 0.23451004017393728 1104 98-99 0.2551869859623679 1104 100 0.2327049617659469 1105 1 0.013439338343083307 1105 2 0.061250209797698574 1105 3 0.06308189810795994 1105 4 0.06796021524455398 1105 5 0.042867416052203566 1105 6 0.07554487607963978 1105 7 0.09660276476012086 1105 8 0.06646957149084187 1105 9 0.03710462158360173 1105 10-11 0.07913383072781954 1105 12-13 0.07578310287939871 1105 14-15 0.09221511319599784 1105 16-17 0.09419265093833928 1105 18-19 0.15305105267372454 1105 20-21 0.10483571076770204 1105 22-23 0.1988800847813934 1105 24-25 0.1286767788031753 1105 26-27 0.1580385198194847 1105 28-29 0.15600189274577758 1105 30-31 0.1851834263876242 1105 32-33 0.14042838365255506 1105 34-35 0.18465000850083868 1105 36-37 0.14154803474727373 1105 38-39 0.17065608609483718 1105 40-41 0.1288921332801607 1105 42-43 0.10022571627008148 1105 44-45 0.06573503748938947 1105 46-47 0.07921658905154771 1105 48-49 0.1030388224373624 1105 50-51 0.039806268956191104 1105 52-53 0.09640413376538248 1105 54-55 0.10781678018070551 1105 56-57 0.04538577407669209 1105 58-59 0.03907486438956198 1105 60-61 0.049221480049340016 1105 62-63 0.06616018410051794 1105 64-65 0.08165090134234276 1105 66-67 0.08819654671493282 1105 68-69 0.07551862843895663 1105 70-71 0.052305468229043584 1105 72-73 0.06621568637304875 1105 74-75 0.053415051891278154 1105 76-77 0.008621738634872145 1105 78-79 0.05709261541021249 1105 80-81 0.11955795937912939 1105 82-83 0.09867459436464543 1105 84-85 0.12285472805989173 1105 86-87 0.04448787480785654 1105 88-89 0.038826075306054975 1105 90-91 0.07278206021735656 1105 92-93 0.13440481625443113 1105 94-95 0.07497268209664298 1105 96-97 0.11321769749390143 1105 98-99 0.10064763776556873 1105 100 0.14976560482222823 1106 1 0.04547794680831885 1106 2 0.026311044903607694 1106 3 0.01963162579937716 1106 4 0.02734376333730637 1106 5 0.028697329738307076 1106 6 0.043268065274311596 1106 7 0.08193855623660795 1106 8 0.04294305474728333 1106 9 0.045856096814006264 1106 10-11 0.10864021051487782 1106 12-13 0.06568194562943575 1106 14-15 0.10961402238677209 1106 16-17 0.11003438615379224 1106 18-19 0.12195176856034351 1106 20-21 0.12585539395859513 1106 22-23 0.10082527265117847 1106 24-25 0.0978189452436169 1106 26-27 0.09020941917111713 1106 28-29 0.12738985447197848 1106 30-31 0.10240762105209456 1106 32-33 0.09412706556680206 1106 34-35 0.091236696927254 1106 36-37 0.08558800549366907 1106 38-39 0.07207033866609436 1106 40-41 0.05754000033569184 1106 42-43 0.11271401531835323 1106 44-45 0.16768957627748904 1106 46-47 0.18785496738576057 1106 48-49 0.14793878237605895 1106 50-51 0.1633769810925969 1106 52-53 0.11522812290650108 1106 54-55 0.1595019633061341 1106 56-57 0.10719061942726427 1106 58-59 0.09755097968343307 1106 60-61 0.09537141168920016 1106 62-63 0.11172573256140339 1106 64-65 0.15922848197433126 1106 66-67 0.14514098158972644 1106 68-69 0.10246059270269114 1106 70-71 0.09085015036179556 1106 72-73 0.11686951242431576 1106 74-75 0.17556826324270247 1106 76-77 0.1402204602166961 1106 78-79 0.12850341306322832 1106 80-81 0.04786171264087358 1106 82-83 0.12251835331569083 1106 84-85 0.13920840144511004 1106 86-87 0.08410456590174675 1106 88-89 0.10603614516233506 1106 90-91 0.0794226886778091 1106 92-93 0.09493695240773548 1106 94-95 0.13390728767982552 1106 96-97 0.11986925176417884 1106 98-99 0.10851166163305237 1106 100 0.13986170273322784 1107 1 -0.03823058642348798 1107 2 -0.012081028120071835 1107 3 -0.00845880592066095 1107 4 -0.0036319181882049634 1107 5 -0.0029832735746424532 1107 6 0.0446592904200358 1107 7 0.032438789061771445 1107 8 0.017364063312520273 1107 9 0.06503326842055657 1107 10-11 0.06157549497868331 1107 12-13 0.05804232257868591 1107 14-15 0.07778164664487974 1107 16-17 0.055051870792638624 1107 18-19 0.06808791364302635 1107 20-21 0.03774318512276409 1107 22-23 0.07435070064753546 1107 24-25 0.11766766955890517 1107 26-27 0.08831980029150088 1107 28-29 0.050629787248453795 1107 30-31 0.10415145876915233 1107 32-33 0.049320128438694155 1107 34-35 0.036647519581215704 1107 36-37 0.06164916115586294 1107 38-39 0.06032391105348012 1107 40-41 0.010997529840786058 1107 42-43 0.07394695727283818 1107 44-45 0.08102685575717317 1107 46-47 0.12273193028389784 1107 48-49 0.11466270102830833 1107 50-51 0.10806399809414557 1107 52-53 0.11448930116867473 1107 54-55 0.22229299475952757 1107 56-57 0.17109547660002278 1107 58-59 0.1625692075426599 1107 60-61 0.15444677727020917 1107 62-63 0.1564789378889273 1107 64-65 0.16603729080438967 1107 66-67 0.1321541343154493 1107 68-69 0.10710837449318689 1107 70-71 0.08429149586372375 1107 72-73 0.08066264006255608 1107 74-75 0.12008866236722326 1107 76-77 0.10973287730590187 1107 78-79 0.14033267562175666 1107 80-81 0.11979797618378285 1107 82-83 0.11087062992108088 1107 84-85 0.15863085473977634 1107 86-87 0.12245310744549442 1107 88-89 0.14748165952393322 1107 90-91 0.1156737181897256 1107 92-93 0.1015109453061882 1107 94-95 0.12596487815932988 1107 96-97 0.12618300376821168 1107 98-99 0.1416495267400748 1107 100 0.12314811711954832 1108 1 -0.030134947755207975 1108 2 0.009586894348629471 1108 3 0.030454076662039142 1108 4 0.017750725659460898 1108 5 0.004966040435029129 1108 6 0.020095083740777397 1108 7 0.024118180769470143 1108 8 0.020426424195171933 1108 9 0.01354757570437215 1108 10-11 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-0.10615413262806328 2214 58-59 -0.10696324995346629 2214 60-61 -0.14591972367501427 2214 62-63 -0.16194628567259173 2214 64-65 -0.21419046268506747 2214 66-67 -0.16684383716913231 2214 68-69 -0.20003341167492295 2214 70-71 -0.15562105994075637 2214 72-73 -0.16259670066681053 2214 74-75 -0.13021141360059119 2214 76-77 -0.14349175625562083 2214 78-79 -0.17471363600369472 2214 80-81 -0.19558990944967292 2214 82-83 -0.16960534650890935 2214 84-85 -0.1180560398136592 2214 86-87 -0.14517707801341828 2214 88-89 -0.11867026172443218 2214 90-91 -0.07688237366635065 2214 92-93 -0.18998192521492285 2214 94-95 -0.2584863044020622 2214 96-97 -0.20120487499315942 2214 98-99 -0.2214282909096994 2214 100 -0.24519648435557428 2215 1 -0.20225988648623883 2215 2 -0.13908928726377923 2215 3 -0.17714826573482156 2215 4 -0.13626508123638104 2215 5 -0.1213915477758647 2215 6 -0.18010846699560545 2215 7 -0.22716978648526265 2215 8 -0.13036572325945173 2215 9 -0.2240490751542481 2215 10-11 -0.18306156456766587 2215 12-13 -0.24375552248586274 2215 14-15 -0.3670040752883139 2215 16-17 -0.3751000444814707 2215 18-19 -0.33281762405073323 2215 20-21 -0.25489932226115997 2215 22-23 -0.32534524025270173 2215 24-25 -0.35123529650229557 2215 26-27 -0.3240232752446559 2215 28-29 -0.33922392387853506 2215 30-31 -0.36438994975852523 2215 32-33 -0.33734421930576985 2215 34-35 -0.29744971467629 2215 36-37 -0.3465368430079323 2215 38-39 -0.3178402902713344 2215 40-41 -0.3435293855861019 2215 42-43 -0.33604718594121863 2215 44-45 -0.368738952248286 2215 46-47 -0.4002885720112772 2215 48-49 -0.3384015670531113 2215 50-51 -0.29271980840605494 2215 52-53 -0.2487381252114531 2215 54-55 -0.3275395973497979 2215 56-57 -0.3159179377864092 2215 58-59 -0.26633380190665434 2215 60-61 -0.24713071111216323 2215 62-63 -0.2624362012916279 2215 64-65 -0.25606867839957204 2215 66-67 -0.32457288604959444 2215 68-69 -0.27600994113819866 2215 70-71 -0.3135028534622748 2215 72-73 -0.26150029408453435 2215 74-75 -0.26502573623302084 2215 76-77 -0.3086112191345336 2215 78-79 -0.3628112524483029 2215 80-81 -0.32161812303544224 2215 82-83 -0.30897205267753947 2215 84-85 -0.28486342878293414 2215 86-87 -0.24866793296021683 2215 88-89 -0.24157097540641814 2215 90-91 -0.25357640788027425 2215 92-93 -0.313693424379224 2215 94-95 -0.32353394009477654 2215 96-97 -0.33624332197125995 2215 98-99 -0.3137903940271016 2215 100 -0.31329420563873356 2216 1 -0.18403286386845252 2216 2 -0.14644607968330092 2216 3 -0.12388144214249763 2216 4 -0.12133892259776502 2216 5 -0.15552285429943424 2216 6 -0.12653056924560957 2216 7 -0.14170066006258963 2216 8 -0.12038194784511091 2216 9 -0.14232647415384037 2216 10-11 -0.1593423174717543 2216 12-13 -0.22043579884875442 2216 14-15 -0.3060059665823829 2216 16-17 -0.2619258577270216 2216 18-19 -0.258607122464241 2216 20-21 -0.3268958545254179 2216 22-23 -0.30967889998301246 2216 24-25 -0.33213475226783373 2216 26-27 -0.2996781984824395 2216 28-29 -0.2752002896052588 2216 30-31 -0.23539991628972246 2216 32-33 -0.27714931114700647 2216 34-35 -0.24875326410411702 2216 36-37 -0.26839872743821047 2216 38-39 -0.2611444456689114 2216 40-41 -0.27767258384211146 2216 42-43 -0.23772488899134459 2216 44-45 -0.2656958554958422 2216 46-47 -0.25717228661066827 2216 48-49 -0.2879574435428154 2216 50-51 -0.34803190099009385 2216 52-53 -0.29789392119582203 2216 54-55 -0.29074352554504657 2216 56-57 -0.23174239450256096 2216 58-59 -0.192568150412896 2216 60-61 -0.22434892488675473 2216 62-63 -0.22450284633843154 2216 64-65 -0.25699136542733925 2216 66-67 -0.2335056963081641 2216 68-69 -0.23805010225542844 2216 70-71 -0.2344061213636266 2216 72-73 -0.2617802462947836 2216 74-75 -0.2790364373390446 2216 76-77 -0.3064895984391427 2216 78-79 -0.3505334019988311 2216 80-81 -0.3218442718167651 2216 82-83 -0.27895285152586524 2216 84-85 -0.30895309971132434 2216 86-87 -0.3702814715159519 2216 88-89 -0.39726950085581336 2216 90-91 -0.4540019672094928 2216 92-93 -0.39274908035116596 2216 94-95 -0.3445108834545252 2216 96-97 -0.47943921658677624 2216 98-99 -0.5844089869324094 2216 100 -0.5248220171554969 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30.0 3 5.0 4 9.0 5 25.0 6 76.0 7 185.0 8 404.0 9 771.0 10 1448.0 11 2394.0 12 3558.0 13 5526.0 14 6390.0 15 8465.0 16 10308.0 17 14134.0 18 17291.0 19 9147.0 20 6383.0 21 6056.0 22 6287.0 23 6966.0 24 7838.0 25 9956.0 26 12742.0 27 16662.0 28 21117.0 29 27533.0 30 35279.0 31 45083.0 32 57468.0 33 75694.0 34 104371.0 35 160218.0 36 294675.0 37 674324.0 38 1221158.0 39 573914.0 40 1783.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.99050034754826 19.538338608982368 13.48495960949164 23.986201433977737 2 15.676940340040444 20.178500723097248 39.80060495208881 24.3439539847735 3 18.233404738886986 25.487284132672116 30.169402686400566 26.10990844204033 4 12.333896564714241 16.881675554345637 38.7228152823577 32.06161259858242 5 12.791554835294445 37.60221542204928 35.83123601140448 13.7749937312518 6 30.978066075450705 38.19495615798044 17.539971216022906 13.287006550545946 7 27.077003534578004 32.48294309994012 22.669040271668266 17.771013093813604 8 25.416944552309538 35.74018577562163 20.33467008661906 18.508199585449763 9 25.315722066487446 16.11096003596395 21.111898894642643 37.46141900290596 10-11 24.033789821551967 26.403427739241298 28.928841644820302 20.633940794386437 12-13 25.071110926660772 23.728296329918713 29.06742746627437 22.133165277146148 14-15 22.096115277619162 25.470689325672886 26.599354057831228 25.833841338876724 16-17 21.439453621263915 28.97043055650885 26.95362307778592 22.63649274444132 18-19 21.30700435156362 27.605801272209835 29.57559920340324 21.511595172823302 20-21 22.470606245950535 26.89512714861903 29.218142467109104 21.41612413832133 22-23 21.91834790815598 26.76967136706148 29.237175491080393 22.074805233702143 24-25 21.86447080088338 26.862574919249084 29.314001386975637 21.958952892891904 26-27 21.70842079219774 26.83205793942683 29.31722227986305 22.142298988512383 28-29 21.7038447719541 26.978441903539114 29.308920249379945 22.00879307512684 30-31 21.982862261495985 27.398528094894736 28.8462869563413 21.772322687267977 32-33 21.636324747008786 27.016405793585175 29.353801129706735 21.993468329699304 34-35 21.696138896523262 26.848064224318442 29.413267016341944 22.042529862816348 36-37 22.098759806865015 27.206107486113744 28.73274683929671 21.962385867724535 38-39 21.986067743514838 27.36320306657074 28.813529316333845 21.837199873580577 40-41 21.871014925979562 27.062472610551797 28.99978813989732 22.066724323571325 42-43 21.83637274924231 26.984554251085346 28.83117463555015 22.34789836412219 44-45 21.99433898689748 27.171760059645823 28.677184399099975 22.156716554356727 46-47 22.16876064559812 27.367585374468213 28.363007749139285 22.10064623079439 48-49 22.159226949278125 27.32276103971561 28.13387979648678 22.384132214519486 50-51 22.16031527077584 27.387392825726643 28.04968724542346 22.402604658074054 52-53 22.27027927490508 27.2819272171213 28.000320982287057 22.447472525686564 54-55 22.267188441851562 27.667715421631712 27.83106522296225 22.234030913554477 56-57 22.005947749539786 27.40644570741333 27.99271724275635 22.59488930029054 58-59 21.954143646248497 27.021092831502003 28.302439030053055 22.722324492196442 60-61 22.112849942522114 27.456102189615788 28.01256822687469 22.418479640987407 62-63 22.060189692985958 27.333716809459286 27.972851747684707 22.633241749870052 64-65 22.02495709836656 27.177622484780183 28.092392980993843 22.70502743585941 66-67 21.903631017801168 27.25805669893806 27.994995462424903 22.84331682083587 68-69 22.275717789703553 27.325600040282506 27.963648230987086 22.435033939026855 70-71 22.202693698065282 27.18125811162525 27.94163228537133 22.674415904938137 72-73 22.13064013206163 27.321555438951677 27.975710922481124 22.572093506505567 74-75 22.135777135844613 27.354953037414948 27.785014722106897 22.724255104633542 76-77 22.185694928102578 27.350535004337324 27.672300882875422 22.791469184684676 78-79 22.290710697155532 27.432725043844847 27.76726056128948 22.50930369771014 80-81 22.277447685836698 27.13504734778953 27.929812260188356 22.657692706185408 82-83 22.032156005630835 27.22693594758988 28.0582835764476 22.68262447033168 84-85 21.98799770030412 27.20311822973335 27.81662682442588 22.99225724553665 86-87 22.111867650357944 27.329879520939528 27.749958571084036 22.808294257618495 88-89 21.89664306992955 27.138598506763767 27.847574452487063 23.117183970819628 90-91 21.832042680427183 27.10452365801967 27.935784908757018 23.12764875279613 92-93 21.94594847164829 27.21980557261004 27.82019914057016 23.014046815171508 94-95 21.778604196529137 27.00704103745672 27.843688446064128 23.37066631995001 96-97 21.73026296505147 26.96393760627478 27.956488540535112 23.34931088813864 98-99 21.676469551127873 27.119852905219144 27.823663877223282 23.380013666429704 100 21.78892193194189 26.93874317150815 27.697666029248857 23.574668867301103 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 27.0 1 26.0 2 78.0 3 137.5 4 111.0 5 90.0 6 195.0 7 462.5 8 812.5 9 984.0 10 844.0 11 725.5 12 838.5 13 999.5 14 1204.0 15 1440.5 16 1653.5 17 1956.0 18 2311.5 19 2631.0 20 3187.0 21 4061.5 22 5202.0 23 6900.0 24 9341.5 25 12502.0 26 16718.5 27 22641.0 28 30199.0 29 38605.0 30 47171.5 31 56798.5 32 67052.0 33 77574.0 34 88119.0 35 96437.5 36 103130.0 37 110088.0 38 115923.5 39 119512.0 40 122424.0 41 125005.0 42 126469.5 43 126137.5 44 124471.0 45 123058.5 46 120460.5 47 116835.0 48 115191.0 49 113803.5 50 110182.0 51 106021.5 52 100684.5 53 97563.5 54 96050.0 55 91159.5 56 83789.5 57 76966.0 58 69477.5 59 60533.5 60 52656.5 61 45369.5 62 39137.5 63 32532.0 64 26288.0 65 21408.5 66 16841.5 67 13121.0 68 10697.5 69 8783.0 70 6732.0 71 4869.5 72 3725.5 73 2883.5 74 2134.5 75 1374.0 76 795.0 77 558.0 78 360.0 79 206.0 80 121.0 81 59.5 82 31.5 83 8.0 84 5.5 85 4.5 86 2.5 87 2.0 88 1.0 89 0.0 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04489688951911572 2 0.2430874897298728 3 0.00278610303415327 4 0.0022927306218552953 5 2.6119715945186904E-4 6 1.7413143963457937E-4 7 0.0 8 3.192409726633955E-4 9 0.0 10-11 5.804381321152645E-4 12-13 0.0 14-15 2.4668620614898745E-4 16-17 1.3059857972593452E-4 18-19 4.353285990864484E-4 20-21 1.4510953302881615E-5 22-23 1.4510953302881614E-4 24-25 0.00134951865716799 26-27 1.5962048633169775E-4 28-29 2.031533462403426E-4 30-31 7.255476651440807E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.706571981728968E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.4668620614898745E-4 70-71 2.902190660576323E-5 72-73 2.6119715945186904E-4 74-75 1.3059857972593452E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.255476651440807E-5 84-85 0.0 86-87 3.4826287926915874E-4 88-89 0.0020170225091005445 90-91 0.0019009348826774914 92-93 4.788614589950933E-4 94-95 4.6435050569221167E-4 96-97 1.7413143963457937E-4 98-99 1.015766731201713E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3445673.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.05941072441991 #Duplication Level Percentage of deduplicated Percentage of total 1 75.07134266307315 45.08740602635177 2 14.51463034255585 17.434802905133793 3 4.759501860895673 8.575586313215224 4 2.078738190758203 4.993911631491379 5 1.0499959512729216 3.153106904823919 6 0.6380722038624538 2.299334433816654 7 0.39629721097380544 1.6660963873972507 8 0.27992256241676156 1.344958731777628 9 0.21056081198691706 1.138154246063065 >10 0.9350771561856331 9.78532735458948 >50 0.046171501323968474 1.8790130891605064 >100 0.018722803195209435 1.9708158211166096 >500 7.733932021732038E-4 0.31173447399565757 >1k 1.450112229550081E-4 0.21430656146198343 >5k 4.833707431833604E-5 0.14544511960505785 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5010 0.14539975209487377 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4159 0.12070210957336927 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.4510953302881615E-5 0.0 16-17 0.0 0.0 0.0 5.804381321152646E-5 0.0 18-19 0.0 0.0 0.0 5.804381321152646E-5 0.0 20-21 0.0 0.0 0.0 5.804381321152646E-5 0.0 22-23 0.0 0.0 0.0 7.255476651440807E-5 0.0 24-25 0.0 0.0 0.0 1.015766731201713E-4 0.0 26-27 0.0 0.0 0.0 1.7413143963457937E-4 0.0 28-29 0.0 0.0 0.0 8.126133849613703E-4 0.0 30-31 0.0 0.0 0.0 0.003961490251686681 0.0 32-33 0.0 0.0 0.0 0.011100879276704435 0.0 34-35 0.0 0.0 0.0 0.021650342327899367 0.0 36-37 0.0 0.0 0.0 0.03990512158292444 0.0 38-39 0.0 0.0 0.0 0.07319324845973486 0.0 40-41 0.0 0.0 0.0 0.11560876496405782 0.0 42-43 0.0 0.0 0.0 0.1569069380640589 0.0 44-45 0.0 0.0 0.0 0.20448835394420772 0.0 46-47 0.0 0.0 0.0 0.26491196349740675 0.0 48-49 0.0 0.0 0.0 0.33118348723166713 0.0 50-51 0.0 0.0 0.0 0.4054215243292094 0.0 52-53 0.0 0.0 0.0 0.47883243708848755 0.0 54-55 0.0 0.0 0.0 0.5607612794365571 0.0 56-57 0.0 0.0 0.0 0.6575348270134747 0.0 58-59 0.0 0.0 0.0 0.7695448755584178 0.0 60-61 0.0 0.0 0.0 0.888273495482595 0.0 62-63 0.0 0.0 0.0 1.0111667590046995 0.0 64-65 0.0 0.0 0.0 1.1410978348787015 0.0 66-67 0.0 0.0 0.0 1.2917215301626128 0.0 68-69 0.0 0.0 0.0 1.4603678294487028 0.0 70-71 0.0 0.0 0.0 1.6487780471333178 0.0 72-73 0.0 0.0 0.0 1.8461415230058105 0.0 74-75 0.0 0.0 0.0 2.044810984675563 0.0 76-77 0.0 0.0 0.0 2.262707459471633 0.0 78-79 0.0 0.0 0.0 2.513210046339278 0.0 80-81 0.0 0.0 0.0 2.7906594734903747 0.0 82-83 0.0 2.902190660576323E-5 0.0 3.0819378391391172 0.0 84-85 0.0 2.902190660576323E-5 0.0 3.373999796266216 0.0 86-87 0.0 2.902190660576323E-5 0.0 3.6811676557816133 0.0 88 0.0 2.902190660576323E-5 0.0 3.937169893951051 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4720 0.0 46.815872 1 GTATCAA 8110 0.0 42.583897 1 ATCAACG 9555 0.0 35.712536 3 TCAACGC 9605 0.0 35.42722 4 CAACGCA 9790 0.0 34.66174 5 TATCAAC 10070 0.0 33.909824 2 AACGCAG 10060 0.0 33.8244 6 ACGCAGA 11600 0.0 29.009789 7 CGCAGAG 11995 0.0 28.015305 8 GTACATG 9290 0.0 26.67671 1 TACATGG 9470 0.0 25.514341 2 GCAGAGT 13305 0.0 24.691748 9 ACATGGG 9415 0.0 24.312151 3 GAGTACT 9125 0.0 20.421185 12-13 AGAGTAC 12620 0.0 19.96144 10-11 CATGGGG 7170 0.0 19.861834 4 GTACGAC 265 2.0971129E-8 19.510193 3 CAGAGTA 13590 0.0 19.314795 10-11 CGTATAC 245 1.9649633E-7 19.18442 3 GTACTTT 10140 0.0 18.284359 14-15 >>END_MODULE