FastQCFastQC Report
Wed 25 May 2016
SRR1033025_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033025_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3568518
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37800.035.5715871
GGTATCA21700.035.5314641
TCAACGC37700.033.9176754
ATCAACG38800.033.077253
CAACGCA39400.032.4542245
AACGCAG42450.030.4439646
GTACATG88000.028.5290661
TATCAAC48350.027.0300522
TACATGG88500.026.8254282
ACATGGG91000.025.0552583
ACGCAGA51750.024.701837
CGCAGAG52550.023.7283738
CATGGGG66450.022.4265674
CTGTCGC8850.018.6125139
GAGTACA62900.018.0881331
GCAGAGT68100.017.830069
GTATTAG15350.017.764321
TAGCGCG1600.001277323117.6290864
TCTGTCG8800.017.1104768
AGTACAT63300.016.8585682
Produced by FastQC (version 0.11.5)