FastQCFastQC Report
Wed 25 May 2016
SRR1033025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3568518
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52290.146531417243797No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44390.12439337562539968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46350.053.395551
GTATCAA81000.042.3101841
ATCAACG93850.035.656863
TCAACGC94750.035.317674
CAACGCA96500.034.628975
AACGCAG99950.033.4336786
TATCAAC102150.033.0730972
ACGCAGA115800.028.6951317
CGCAGAG118700.028.073268
GTACATG86800.027.7521551
TACATGG87850.026.710482
TATACCG1807.508788E-926.111085
ACATGGG90550.025.0700993
GCAGAGT136850.023.9718699
GAGTACT95300.021.13123112-13
CAGAGTA133550.020.69291310-11
AGAGTAC123000.020.00321410-11
CATGGGG67600.019.8843124
GTGGTAT22600.019.607171
TAGACCG1456.60368E-419.4482525
Produced by FastQC (version 0.11.5)