##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033025_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3568518 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.709479397329645 34.0 31.0 34.0 31.0 34.0 2 32.714076824048526 34.0 31.0 34.0 31.0 34.0 3 32.81329924635381 34.0 31.0 34.0 31.0 34.0 4 36.303490973003356 37.0 37.0 37.0 35.0 37.0 5 36.28689052430168 37.0 37.0 37.0 35.0 37.0 6 36.35445638777778 37.0 37.0 37.0 35.0 37.0 7 36.30265785404473 37.0 37.0 37.0 35.0 37.0 8 36.31161059016656 37.0 37.0 37.0 35.0 37.0 9 38.120163048077664 39.0 39.0 39.0 37.0 39.0 10-11 38.07675188972004 39.0 39.0 39.0 36.0 39.0 12-13 38.052408450791056 39.0 39.0 39.0 35.0 39.0 14-15 39.55395671256247 41.0 40.0 41.0 37.0 41.0 16-17 39.54946661331118 41.0 40.0 41.0 37.0 41.0 18-19 39.564478447355455 41.0 40.0 41.0 37.0 41.0 20-21 39.55348550855005 41.0 40.0 41.0 37.0 41.0 22-23 39.48007772414206 41.0 39.0 41.0 36.5 41.0 24-25 39.4231012706115 41.0 39.0 41.0 36.0 41.0 26-27 39.313432775174455 41.0 39.0 41.0 36.0 41.0 28-29 39.262603411276054 41.0 39.0 41.0 36.0 41.0 30-31 39.26502304317927 41.0 39.0 41.0 36.0 41.0 32-33 39.22691310510413 41.0 39.0 41.0 35.5 41.0 34-35 39.14879061840237 41.0 39.0 41.0 35.0 41.0 36-37 39.06126955223429 41.0 39.0 41.0 35.0 41.0 38-39 38.96928725033754 41.0 39.0 41.0 35.0 41.0 40-41 38.86981052078202 41.0 39.0 41.0 35.0 41.0 42-43 38.755390753248264 41.0 39.0 41.0 35.0 41.0 44-45 38.59725732082618 41.0 38.0 41.0 35.0 41.0 46-47 38.41008087951357 41.0 38.0 41.0 34.5 41.0 48-49 38.21243104280265 40.0 38.0 41.0 34.0 41.0 50-51 38.08004541381044 40.0 38.0 41.0 34.0 41.0 52-53 37.94151843986775 40.0 38.0 41.0 34.0 41.0 54-55 37.78885450486729 40.0 37.0 41.0 33.0 41.0 56-57 37.63755696342291 40.0 37.0 41.0 33.0 41.0 58-59 37.45599083429032 40.0 37.0 41.0 33.0 41.0 60-61 37.26256319850425 40.0 36.0 41.0 33.0 41.0 62-63 37.029426641535785 39.0 36.0 41.0 32.5 41.0 64-65 36.760516550568056 39.0 35.0 41.0 32.0 41.0 66-67 36.483867252456065 39.0 35.0 41.0 32.0 41.0 68-69 36.17832710946112 38.0 35.0 40.5 31.5 41.0 70-71 35.85146172724924 37.0 35.0 40.0 31.0 41.0 72-73 35.505584811397895 37.0 35.0 39.0 31.0 41.0 74-75 35.15962102475034 36.0 35.0 39.0 31.0 41.0 76-77 34.80603124322198 36.0 35.0 39.0 31.0 40.5 78-79 34.44649445512114 35.0 34.5 37.5 30.0 39.5 80-81 34.11758004303187 35.0 34.0 37.0 30.0 39.0 82-83 33.816693932887546 35.0 34.0 36.5 30.0 39.0 84-85 33.59278193356458 35.0 34.0 36.0 30.0 37.5 86-87 33.38269808363024 35.0 34.0 36.0 30.0 37.0 88-89 33.173384861726916 35.0 34.0 35.5 30.0 37.0 90-91 32.98977838979655 35.0 34.0 35.0 30.0 36.0 92-93 32.81653700499759 35.0 34.0 35.0 29.0 36.0 94-95 32.661364325470686 35.0 34.0 35.0 29.0 36.0 96-97 32.51993236407943 35.0 34.0 35.0 29.0 36.0 98-99 32.39057095970932 35.0 34.0 35.0 29.0 35.5 100 32.28373291097313 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12485748508836991 1101 2 0.14264385468941754 1101 3 0.1612108888230992 1101 4 0.11677269910958898 1101 5 0.1088192846035625 1101 6 0.13621109168840917 1101 7 0.18316349459569636 1101 8 0.14774905315770326 1101 9 0.2176987771601091 1101 10-11 0.15944442722928898 1101 12-13 0.17401263574018344 1101 14-15 0.27060086165327846 1101 16-17 0.27559068968722045 1101 18-19 0.24385894865692848 1101 20-21 0.25368861506579776 1101 22-23 0.21416323507819612 1101 24-25 0.2741067094265972 1101 26-27 0.24293287035908406 1101 28-29 0.22889709295017013 1101 30-31 0.2648074720728175 1101 32-33 0.23692687368402687 1101 34-35 0.2596807017419067 1101 36-37 0.25225853670222165 1101 38-39 0.22188780589554113 1101 40-41 0.24709891760111447 1101 42-43 0.2591953006925607 1101 44-45 0.23642621313052103 1101 46-47 0.19766380426116825 1101 48-49 0.13682999313921584 1101 50-51 0.15351980384141228 1101 52-53 0.17224142008103627 1101 54-55 0.1554734424167492 1101 56-57 0.12591253863288898 1101 58-59 0.2048174517895518 1101 60-61 0.19330234710041339 1101 62-63 0.17248915783111585 1101 64-65 0.16124173739457603 1101 66-67 0.15885005783717077 1101 68-69 0.1306839262666628 1101 70-71 0.06515562646567474 1101 72-73 0.13935972668421925 1101 74-75 0.18819111670089228 1101 76-77 0.19467125756996495 1101 78-79 0.17381304790642815 1101 80-81 0.17042582024151187 1101 82-83 0.19588584836036915 1101 84-85 0.23279083692222713 1101 86-87 0.19349650263155382 1101 88-89 0.2249555813556796 1101 90-91 0.2525435419034707 1101 92-93 0.24614668941048734 1101 94-95 0.20224491361706498 1101 96-97 0.196265244799271 1101 98-99 0.22023327279192273 1101 100 0.21143179206874407 1102 1 0.20302400867340253 1102 2 0.21119509696032424 1102 3 0.18001562487410183 1102 4 0.17395847376724305 1102 5 0.17610769713966334 1102 6 0.17410135539812188 1102 7 0.21760856736222678 1102 8 0.20699705904895893 1102 9 0.27814564113293017 1102 10-11 0.27899743628982776 1102 12-13 0.26698835288194545 1102 14-15 0.33359389509140414 1102 16-17 0.36731871542434646 1102 18-19 0.3308562462208329 1102 20-21 0.3496895565722582 1102 22-23 0.2943018924157528 1102 24-25 0.3477976589514995 1102 26-27 0.4009250296807281 1102 28-29 0.35482407150550443 1102 30-31 0.3087210820176196 1102 32-33 0.33355214922605114 1102 34-35 0.32967570120962364 1102 36-37 0.29183159672521697 1102 38-39 0.34160660408792864 1102 40-41 0.3857608943154318 1102 42-43 0.35044402736365754 1102 44-45 0.26946342119270383 1102 46-47 0.3151431062571888 1102 48-49 0.29845584003112435 1102 50-51 0.302903624970817 1102 52-53 0.2727064598167033 1102 54-55 0.2895922109184852 1102 56-57 0.32248253485318656 1102 58-59 0.3681846949824319 1102 60-61 0.29187533973342283 1102 62-63 0.2988439162966188 1102 64-65 0.39123228326322845 1102 66-67 0.3461395251730508 1102 68-69 0.29404091446169645 1102 70-71 0.2554468781711776 1102 72-73 0.22403011640344062 1102 74-75 0.25894596267571046 1102 76-77 0.25428478088445416 1102 78-79 0.27372358525854423 1102 80-81 0.28399674722494694 1102 82-83 0.1943727478447883 1102 84-85 0.27691150015689203 1102 86-87 0.28194927738979914 1102 88-89 0.27994971647357403 1102 90-91 0.26673987725557424 1102 92-93 0.2780731835392558 1102 94-95 0.3208168612169615 1102 96-97 0.3445024595027846 1102 98-99 0.2921042043656925 1102 100 0.2754825552847535 1103 1 0.1346818101517684 1103 2 0.13268502510899793 1103 3 0.17007771966900975 1103 4 0.11040780928946248 1103 5 0.1380350264469996 1103 6 0.1374131276785775 1103 7 0.145074903221726 1103 8 0.15043744256555414 1103 9 0.18105440841152642 1103 10-11 0.23678733759825832 1103 12-13 0.21466675685128678 1103 14-15 0.2949866090940958 1103 16-17 0.28735041437688835 1103 18-19 0.301779563776968 1103 20-21 0.3136784147037801 1103 22-23 0.33659381282220835 1103 24-25 0.31035279826291173 1103 26-27 0.3170874479743375 1103 28-29 0.3246030754965048 1103 30-31 0.3079677865412336 1103 32-33 0.27520956763678583 1103 34-35 0.3289894531971882 1103 36-37 0.303402832572381 1103 38-39 0.3306873059783655 1103 40-41 0.3147465121029711 1103 42-43 0.34220637271710785 1103 44-45 0.36114670410864136 1103 46-47 0.35952965406149673 1103 48-49 0.32784099607974326 1103 50-51 0.31186073249305224 1103 52-53 0.31727765387081774 1103 54-55 0.3115536353705437 1103 56-57 0.29806229240326587 1103 58-59 0.3366450279893627 1103 60-61 0.3506412301843227 1103 62-63 0.36258054501492865 1103 64-65 0.36868492689966104 1103 66-67 0.3715484094764676 1103 68-69 0.3302863398115292 1103 70-71 0.37046295489456327 1103 72-73 0.3752983784936319 1103 74-75 0.38446415092653297 1103 76-77 0.3987921331682003 1103 78-79 0.35362360588298003 1103 80-81 0.34844052048615737 1103 82-83 0.37056174873595893 1103 84-85 0.36327192870114544 1103 86-87 0.32135050399701015 1103 88-89 0.3337133431691157 1103 90-91 0.3092784107340947 1103 92-93 0.33367504591968355 1103 94-95 0.407680740962455 1103 96-97 0.40976689457306037 1103 98-99 0.4672983135763644 1103 100 0.5088032231523201 1104 1 0.09659147026665948 1104 2 0.16253454871583273 1104 3 0.1672249345298411 1104 4 0.1085996941376095 1104 5 0.09585721466325481 1104 6 0.07084645206001028 1104 7 0.10666871323535787 1104 8 0.11544226476897279 1104 9 0.18028420709509163 1104 10-11 0.20234785329532912 1104 12-13 0.1623517614032437 1104 14-15 0.24096990788660122 1104 16-17 0.21731403249891912 1104 18-19 0.24134662956330288 1104 20-21 0.2345060264209735 1104 22-23 0.252536726716734 1104 24-25 0.26687738305514586 1104 26-27 0.2812384614815784 1104 28-29 0.28289186045325465 1104 30-31 0.2657705508207826 1104 32-33 0.22808456362532326 1104 34-35 0.2996394625713492 1104 36-37 0.24292429433341312 1104 38-39 0.2502244926829391 1104 40-41 0.22693385006088818 1104 42-43 0.19929180256882972 1104 44-45 0.13656672562698446 1104 46-47 0.16881024211696172 1104 48-49 0.15439959809678783 1104 50-51 0.14499898194031147 1104 52-53 0.18016915176036719 1104 54-55 0.19468529756970554 1104 56-57 0.15243808308416362 1104 58-59 0.1657316975949641 1104 60-61 0.19857503002995003 1104 62-63 0.12482438129310225 1104 64-65 0.18647024341693452 1104 66-67 0.20147495321494802 1104 68-69 0.25372520423751865 1104 70-71 0.18187384209554835 1104 72-73 0.16079688960665806 1104 74-75 0.15001301226158148 1104 76-77 0.16173396438939136 1104 78-79 0.157547410001861 1104 80-81 0.19173411075594515 1104 82-83 0.22274812394223176 1104 84-85 0.2368634821600395 1104 86-87 0.2322773973663601 1104 88-89 0.2444333293865597 1104 90-91 0.24775309121033473 1104 92-93 0.2150108384923044 1104 94-95 0.2781923336746033 1104 96-97 0.3502802438846899 1104 98-99 0.33402899478226544 1104 100 0.3083355285371425 1105 1 0.039229247329451766 1105 2 0.0901835113074867 1105 3 0.08576963354044409 1105 4 0.050644980036885556 1105 5 0.04115125469608927 1105 6 0.05545860359413979 1105 7 0.043865678930551155 1105 8 0.034351696857847 1105 9 0.051087289878523734 1105 10-11 0.0609699946283655 1105 12-13 0.08161432142698999 1105 14-15 0.1254664310313771 1105 16-17 0.0879439528224566 1105 18-19 0.13822012389599792 1105 20-21 0.1348084118667714 1105 22-23 0.10984908755505529 1105 24-25 0.10647297753691021 1105 26-27 0.14811466101321002 1105 28-29 0.0983430398445364 1105 30-31 0.06671924556800946 1105 32-33 0.09415777870281516 1105 34-35 0.12040179586603728 1105 36-37 0.12990128825139635 1105 38-39 0.09888353265516514 1105 40-41 0.1088407683499284 1105 42-43 0.08823115191703579 1105 44-45 0.006863355379003622 1105 46-47 0.008298074552868684 1105 48-49 0.04808139215363383 1105 50-51 0.07927408123574509 1105 52-53 0.11756391940710387 1105 54-55 0.11646360114242782 1105 56-57 0.12061500766857591 1105 58-59 0.12153433789656987 1105 60-61 0.11518830160333948 1105 62-63 0.1775478054575501 1105 64-65 0.15871148359172338 1105 66-67 0.12745906365841364 1105 68-69 0.12726658634949928 1105 70-71 0.12791021112858658 1105 72-73 0.1437617396281894 1105 74-75 0.1273930442346085 1105 76-77 0.13365569152743006 1105 78-79 0.09994793905919863 1105 80-81 0.10485789293049663 1105 82-83 0.14439511217312884 1105 84-85 0.08564880667301367 1105 86-87 0.03545096389103719 1105 88-89 0.040162796686466606 1105 90-91 0.10764545264778036 1105 92-93 0.17216830782557224 1105 94-95 0.0660161257506715 1105 96-97 0.06426976975684084 1105 98-99 0.03621597548068678 1105 100 0.056912898027299264 1106 1 0.0631330991385255 1106 2 0.08124070974808006 1106 3 0.07136262518361036 1106 4 0.039786762796957476 1106 5 0.08529191844801431 1106 6 0.09740029825076846 1106 7 0.0843372360466148 1106 8 0.09103244855968029 1106 9 0.08017493213132099 1106 10-11 0.1346888631723715 1106 12-13 0.0779147299387759 1106 14-15 0.15408630057097383 1106 16-17 0.15318810622636647 1106 18-19 0.10859318393742257 1106 20-21 0.12775604021907583 1106 22-23 0.1573738836239471 1106 24-25 0.11973401748225143 1106 26-27 0.14173365734262688 1106 28-29 0.1770536737877464 1106 30-31 0.18485668456877846 1106 32-33 0.12664080978584735 1106 34-35 0.15154406549797983 1106 36-37 0.1694894489681289 1106 38-39 0.12756758277374303 1106 40-41 0.17216843578189156 1106 42-43 0.17104607668071736 1106 44-45 0.16193603481003294 1106 46-47 0.13330056574842786 1106 48-49 0.17252674130188694 1106 50-51 0.10342511500439855 1106 52-53 0.15148376137008768 1106 54-55 0.1881618701933192 1106 56-57 0.13245816982023229 1106 58-59 0.16259540414893792 1106 60-61 0.12073994013832134 1106 62-63 0.05188544269105222 1106 64-65 0.17445539665985166 1106 66-67 0.14647125922324022 1106 68-69 0.172716401048838 1106 70-71 0.12509862936481397 1106 72-73 0.14285328364683636 1106 74-75 0.1481225223929883 1106 76-77 0.1710833029204082 1106 78-79 0.1234754358055099 1106 80-81 0.11781186995628445 1106 82-83 0.12093373360234239 1106 84-85 0.11331906937124359 1106 86-87 0.0390593591684123 1106 88-89 0.08397550838272849 1106 90-91 0.11716396027475184 1106 92-93 0.14028740793634853 1106 94-95 0.12883898469542032 1106 96-97 0.13830751133269104 1106 98-99 0.11552004911319358 1106 100 0.14262213070068697 1107 1 0.016127363249900384 1107 2 0.019961633415647384 1107 3 0.01926922986847046 1107 4 0.01616122951192267 1107 5 0.0259227320375075 1107 6 0.03256340297587457 1107 7 0.02868818791009886 1107 8 0.02783307094915699 1107 9 0.006171990678645045 1107 10-11 0.03940049795373568 1107 12-13 0.06846499177390086 1107 14-15 0.08856927821324234 1107 16-17 0.09700968604695959 1107 18-19 0.07936679521623802 1107 20-21 0.09379985623685627 1107 22-23 0.12023940607557648 1107 24-25 0.09422581053858892 1107 26-27 0.08989432706813716 1107 28-29 0.10077700394595723 1107 30-31 0.10192862550270831 1107 32-33 0.07329137867912294 1107 34-35 0.0808323004698579 1107 36-37 0.07444662405556102 1107 38-39 0.06486071821024808 1107 40-41 0.0630880282492754 1107 42-43 0.11583378144204204 1107 44-45 0.09344747863735137 1107 46-47 0.09221124022542426 1107 48-49 0.03907009977899634 1107 50-51 0.008603199504925385 1107 52-53 0.06944769000379836 1107 54-55 0.07887176816482366 1107 56-57 0.0929030170234455 1107 58-59 0.07539582418078083 1107 60-61 0.0997981610010612 1107 62-63 0.11737601740909298 1107 64-65 0.09281834575963899 1107 66-67 0.024076369200706438 1107 68-69 0.10576159835311927 1107 70-71 0.14672692913584484 1107 72-73 0.11762363288704591 1107 74-75 0.10585870505596517 1107 76-77 0.07155178599839473 1107 78-79 0.09860071446650664 1107 80-81 0.06449010929952692 1107 82-83 0.11115460528301213 1107 84-85 0.09698347684349784 1107 86-87 0.0866408640160472 1107 88-89 0.02349153823774941 1107 90-91 0.04222204026974907 1107 92-93 0.09704915054862084 1107 94-95 0.1199852792029148 1107 96-97 0.1271865059394699 1107 98-99 0.15816779394356928 1107 100 0.09765233900606773 1108 1 -0.01213727356906702 1108 2 0.035314017447554136 1108 3 0.02086116789093495 1108 4 0.022977989761976403 1108 5 0.0126442427965614 1108 6 0.02991317432987728 1108 7 0.029747679090206702 1108 8 0.005912041693328263 1108 9 0.009357467465683555 1108 10-11 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-0.22301513028710218 2214 68-69 -0.2807626213775549 2214 70-71 -0.193058449254643 2214 72-73 -0.2587398533721199 2214 74-75 -0.2500924200007546 2214 76-77 -0.25135528104764404 2214 78-79 -0.16480239671083297 2214 80-81 -0.23938517613450472 2214 82-83 -0.25930897512407824 2214 84-85 -0.20360702321271873 2214 86-87 -0.23584296577941188 2214 88-89 -0.22781861277524484 2214 90-91 -0.1857215992684047 2214 92-93 -0.23657003654638942 2214 94-95 -0.2694598304485609 2214 96-97 -0.20281054491812966 2214 98-99 -0.25099517529515225 2214 100 -0.23797147176991018 2215 1 -0.26819582253241236 2215 2 -0.21298570879208256 2215 3 -0.2614035654061695 2215 4 -0.21057104029207352 2215 5 -0.1857131727917718 2215 6 -0.2258841104796261 2215 7 -0.2408601147196805 2215 8 -0.2500577886339457 2215 9 -0.32354715177350357 2215 10-11 -0.2514291223962388 2215 12-13 -0.2819000696914884 2215 14-15 -0.4523333297737153 2215 16-17 -0.426563779694078 2215 18-19 -0.40073229201522764 2215 20-21 -0.45727949782731514 2215 22-23 -0.4139600789707245 2215 24-25 -0.46744344725924236 2215 26-27 -0.48422793845915635 2215 28-29 -0.512897836439457 2215 30-31 -0.5558013004034876 2215 32-33 -0.45755123543150944 2215 34-35 -0.5235833086745956 2215 36-37 -0.5267105704128667 2215 38-39 -0.5012543412378321 2215 40-41 -0.5241881224505605 2215 42-43 -0.5444019319214704 2215 44-45 -0.5237935853223448 2215 46-47 -0.5519941465927971 2215 48-49 -0.5522176251630242 2215 50-51 -0.5402214109512329 2215 52-53 -0.5454167227822211 2215 54-55 -0.5368967386980898 2215 56-57 -0.4455440683126284 2215 58-59 -0.44414219573775426 2215 60-61 -0.4329025698503486 2215 62-63 -0.43475797298668795 2215 64-65 -0.49073781700822394 2215 66-67 -0.486707196930972 2215 68-69 -0.4772692495564499 2215 70-71 -0.458450721371662 2215 72-73 -0.4101700592776041 2215 74-75 -0.4780449965407598 2215 76-77 -0.49664388340555377 2215 78-79 -0.5035905509110492 2215 80-81 -0.4299215313347915 2215 82-83 -0.4379192838703787 2215 84-85 -0.4796559235472344 2215 86-87 -0.45597845241844936 2215 88-89 -0.39683543741009686 2215 90-91 -0.4349858399518567 2215 92-93 -0.4455613401255505 2215 94-95 -0.4852946100329447 2215 96-97 -0.49112961968287294 2215 98-99 -0.5391331148351632 2215 100 -0.4899811849412714 2216 1 -0.226719888053168 2216 2 -0.19623015731030335 2216 3 -0.20526009972819992 2216 4 -0.18683835172392804 2216 5 -0.20172211171335874 2216 6 -0.20173660200243404 2216 7 -0.21740895951538164 2216 8 -0.1822262816291058 2216 9 -0.20897419119775407 2216 10-11 -0.27166528771385856 2216 12-13 -0.2852834191511917 2216 14-15 -0.393980263342506 2216 16-17 -0.35265279720817233 2216 18-19 -0.3589091752379545 2216 20-21 -0.3707100449159313 2216 22-23 -0.36595723932099133 2216 24-25 -0.4279534777836176 2216 26-27 -0.39868410042856794 2216 28-29 -0.4525897198899784 2216 30-31 -0.36725527898652643 2216 32-33 -0.3687821025524656 2216 34-35 -0.38958398102815295 2216 36-37 -0.39333912228008927 2216 38-39 -0.4224046380207298 2216 40-41 -0.4086011400760228 2216 42-43 -0.34011105882725445 2216 44-45 -0.41376497730212947 2216 46-47 -0.30901858254981107 2216 48-49 -0.3433558461578059 2216 50-51 -0.2959679186539219 2216 52-53 -0.380139305075474 2216 54-55 -0.35972730469721625 2216 56-57 -0.37489013170875296 2216 58-59 -0.35970150838596027 2216 60-61 -0.37047576671706395 2216 62-63 -0.39741218131613465 2216 64-65 -0.45736648638816746 2216 66-67 -0.4759060602756122 2216 68-69 -0.43740974776699915 2216 70-71 -0.3770883701698935 2216 72-73 -0.36523030172261883 2216 74-75 -0.4565309707646179 2216 76-77 -0.4473075681416958 2216 78-79 -0.451116465178103 2216 80-81 -0.3812087566533222 2216 82-83 -0.3459390706625243 2216 84-85 -0.45386447988355627 2216 86-87 -0.4805997405531315 2216 88-89 -0.5830218711629342 2216 90-91 -0.5312648041260246 2216 92-93 -0.5253423994611595 2216 94-95 -0.433348337211072 2216 96-97 -0.510821904484537 2216 98-99 -0.6315785202323596 2216 100 -0.6421241379021936 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19.0 3 5.0 4 12.0 5 24.0 6 82.0 7 180.0 8 410.0 9 847.0 10 1476.0 11 2345.0 12 3721.0 13 5760.0 14 6840.0 15 8740.0 16 10610.0 17 14626.0 18 17387.0 19 9639.0 20 6715.0 21 6288.0 22 6743.0 23 7397.0 24 8570.0 25 10379.0 26 13189.0 27 17187.0 28 22319.0 29 28326.0 30 36836.0 31 46482.0 32 60655.0 33 78645.0 34 109910.0 35 168199.0 36 306890.0 37 692870.0 38 1251211.0 39 605147.0 40 1837.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66889420759923 19.789966701832956 13.47724877574496 24.063890314822856 2 15.644202110774952 20.253748072239404 40.17346333954149 23.928586477444146 3 17.989766284674843 25.459554573603132 30.256913390619495 26.293765751102526 4 12.147077896056016 16.952591369518515 39.08472708665063 31.815603647774843 5 12.418386737086406 37.89167290410248 36.27578704483415 13.414153313976964 6 30.804250732938137 38.57409554789473 17.48377616004869 13.137877559118447 7 26.86426129838773 32.58845828996799 22.857471925320258 17.689808486324015 8 24.971437618834777 36.26894175572124 20.435672641624432 18.323947983819554 9 25.06460665183698 16.035144000955018 21.268661108056623 37.631588239151384 10-11 23.78619330925128 26.489223957049674 29.278369351557075 20.446213382141963 12-13 24.808800179794524 23.969670322526046 29.254006845418743 21.967522652260687 14-15 21.896823280650725 25.791055282620174 26.674029219770667 25.63809221695843 16-17 21.08428045959747 29.224825795835464 27.190505627832763 22.500388116734303 18-19 20.94213122862375 27.86487618764721 29.767745876774725 21.42524670695431 20-21 22.088931595693225 27.170368763727687 29.469180203098315 21.27151943748077 22-23 21.72717623939947 26.988626213303164 29.539170732903415 21.745026814393952 24-25 21.60439190595082 27.11908887651821 29.477057907253617 21.799461310277348 26-27 21.322219651902774 27.05337192829117 29.62546460158702 21.99894381821904 28-29 21.37752470604324 27.11606904787559 29.68236458386898 21.824041662212192 30-31 21.687793793303182 27.52704099868951 29.210429600087316 21.574735607919987 32-33 21.33340787408106 27.241000325625368 29.683428807140665 21.7421629931529 34-35 21.53472393862102 27.00465571422086 29.634893252605142 21.825727094552978 36-37 21.850541317151826 27.41916952639723 29.030146408116757 21.700142748334184 38-39 21.640384047383254 27.631876874377543 29.08804159037449 21.63969748786471 40-41 21.52364197381236 27.279690952722497 29.329086562745772 21.86758051071937 42-43 21.579126124626526 27.215331406483028 29.16315680627084 22.04238566261961 44-45 21.696878087766404 27.437636576304225 29.015140178639985 21.850345157289386 46-47 21.774431290524525 27.656957313932562 28.70950910153739 21.859102294005524 48-49 21.79963783284826 27.58051101325536 28.482173832386444 22.137677321509937 50-51 21.812962692075534 27.562534363004477 28.350018130775855 22.274484814144135 52-53 21.905396021541716 27.520205866973345 28.377536557192652 22.196861554292287 54-55 22.022013059763186 27.902143130565687 28.148589974885933 21.927253834785194 56-57 21.738534035697732 27.629901264334382 28.326534992957864 22.305029707010025 58-59 21.64378882213849 27.280414446557366 28.648419315805608 22.427377415498533 60-61 21.94509037084863 27.710144659491696 28.202772131176022 22.141992838483652 62-63 21.735493557829887 27.631372463302696 28.319641374934918 22.3134926039325 64-65 21.767131341357953 27.400310156765357 28.40251891681645 22.43003958506024 66-67 21.666305172063023 27.49345806858758 28.268163422462774 22.57207333688663 68-69 22.09698947263999 27.608377836021802 28.148140779165313 22.14649191217289 70-71 21.99515625186089 27.444534039695757 28.21527427005752 22.34503543838583 72-73 21.90887006309918 27.600841135611542 28.165923078798453 22.32436572249082 74-75 21.937083343783527 27.632324318704605 28.085355965346366 22.345236372165505 76-77 21.924367482523557 27.63738336194465 27.949936079907683 22.488313075624113 78-79 22.05981586753941 27.72478659208108 27.98057905270479 22.234818487674715 80-81 21.926679366616618 27.520528129604504 28.099816226231727 22.452976277547148 82-83 21.859843139276943 27.584505716101592 28.16739507403077 22.388256070590685 84-85 21.854996948312998 27.54518822659715 27.989784554820794 22.610030270269057 86-87 21.958385876418664 27.592714025500907 27.97042174583158 22.478478352248842 88-89 21.701398570631504 27.540438663624805 28.024321066777564 22.733841698966128 90-91 21.630599740418454 27.407809388736325 28.146550498110447 22.815040372734774 92-93 21.749528793346666 27.639121557585295 27.907203534932624 22.704146114135416 94-95 21.548881133059183 27.38584589568974 28.11225785859235 22.95301511265873 96-97 21.53985729348013 27.39296835866485 28.07044952203474 22.996724825820284 98-99 21.54157660237149 27.473749238303714 27.963070357968178 23.021603801356612 100 21.62903479819914 27.35566977664117 27.890037264769298 23.12525816039039 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 34.0 1 30.5 2 91.0 3 160.5 4 113.0 5 89.0 6 203.0 7 522.5 8 936.5 9 1127.0 10 956.0 11 778.5 12 859.5 13 1013.0 14 1206.0 15 1471.5 16 1748.0 17 2020.0 18 2366.5 19 2844.0 20 3511.5 21 4408.5 22 5717.5 23 7808.5 24 10871.0 25 14603.0 26 19278.5 27 25705.0 28 34273.0 29 44450.5 30 54983.5 31 65766.5 32 76275.0 33 86983.5 34 98213.5 35 107222.5 36 114068.0 37 120827.0 38 126410.5 39 129402.0 40 130565.5 41 131234.5 42 130367.5 43 128309.5 44 125792.5 45 123478.5 46 122391.5 47 119682.0 48 115756.0 49 112552.0 50 109594.0 51 105686.5 52 98860.0 53 93570.5 54 90840.0 55 86770.5 56 81391.0 57 73932.0 58 66449.5 59 60781.0 60 54219.0 61 46299.5 62 39432.5 63 32915.5 64 26681.5 65 21929.0 66 17629.5 67 13424.0 68 10198.0 69 7995.0 70 5908.5 71 4159.0 72 3075.0 73 2241.0 74 1669.5 75 1182.5 76 788.0 77 535.0 78 355.0 79 262.0 80 150.5 81 57.0 82 30.5 83 20.0 84 9.5 85 6.0 86 5.0 87 2.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04637779604866782 2 0.24346241212738737 3 0.003278671986522136 4 0.00269019239919765 5 3.0825121240806407E-4 6 1.1209134996656875E-4 7 0.0 8 3.6429688739134846E-4 9 0.0 10-11 6.585366810535915E-4 12-13 0.0 14-15 2.662169561706008E-4 16-17 1.1209134996656875E-4 18-19 3.6429688739134846E-4 20-21 0.0 22-23 1.5412560620403203E-4 24-25 0.0012470162683780775 26-27 9.807993122074766E-5 28-29 2.381941186789586E-4 30-31 4.203425623746328E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.005709372910547E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.961598624414953E-4 70-71 1.4011418745821094E-5 72-73 2.5220553742477973E-4 74-75 9.807993122074766E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.604567498328438E-5 84-85 0.0 86-87 5.044110748495595E-4 88-89 0.002494032536756155 90-91 0.0023118840930604805 92-93 5.604567498328438E-4 94-95 5.464453310870227E-4 96-97 1.5412560620403203E-4 98-99 7.005709372910547E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3568518.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.59458649420749 #Duplication Level Percentage of deduplicated Percentage of total 1 72.26100514377883 42.3410371605552 2 15.612337258325926 18.295968917194333 3 5.564305510835116 9.781145416044708 4 2.466254062689106 5.780365479717098 5 1.3030681130746826 3.8176368629699122 6 0.7387260863186174 2.5971209736194165 7 0.4790514907399293 1.9648876806537878 8 0.3367674694519913 1.5786200493792066 9 0.2260489236535091 1.1920718888044242 >10 0.9591144314608073 9.437799224511107 >50 0.0395504848249635 1.5484488196928563 >100 0.013339743148865118 1.212438384023801 >500 2.3960094539052533E-4 0.08743687429037986 >1k 1.4376056418422602E-4 0.21819908068105312 >5k 4.792018806140868E-5 0.14682318786272383 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5229 0.146531417243797 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4439 0.12439337562539968 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 8.406851247492657E-5 0.0 4 0.0 0.0 0.0 8.406851247492657E-5 0.0 5 0.0 0.0 0.0 1.1209134996656875E-4 0.0 6 0.0 0.0 0.0 1.1209134996656875E-4 0.0 7 0.0 0.0 0.0 1.1209134996656875E-4 0.0 8 0.0 0.0 0.0 1.1209134996656875E-4 0.0 9 0.0 0.0 0.0 1.4011418745821095E-4 0.0 10-11 0.0 0.0 0.0 1.4011418745821095E-4 0.0 12-13 0.0 0.0 0.0 1.4011418745821095E-4 0.0 14-15 0.0 0.0 0.0 1.8214844369567423E-4 0.0 16-17 0.0 0.0 0.0 2.381941186789586E-4 0.0 18-19 0.0 0.0 0.0 2.522055374247797E-4 0.0 20-21 0.0 0.0 0.0 2.522055374247797E-4 0.0 22-23 0.0 0.0 0.0 2.662169561706008E-4 0.0 24-25 0.0 0.0 0.0 3.2226263115388515E-4 0.0 26-27 0.0 0.0 0.0 4.3435398112045393E-4 0.0 28-29 0.0 0.0 0.0 0.0010928906621740454 0.0 30-31 0.0 0.0 0.0 0.004147379948763044 0.0 32-33 0.0 0.0 0.0 0.010788792434282244 0.0 34-35 0.0 0.0 0.0 0.02124131081866478 0.0 36-37 0.0 0.0 0.0 0.04032486315047311 0.0 38-39 0.0 0.0 0.0 0.07341983422810253 0.0 40-41 0.0 0.0 0.0 0.11256773820392667 0.0 42-43 0.0 0.0 0.0 0.15292062419189142 0.0 44-45 0.0 0.0 0.0 0.20027921955276673 0.0 46-47 0.0 0.0 0.0 0.25803428762304126 0.0 48-49 0.0 0.0 0.0 0.32195437994147713 0.0 50-51 0.0 0.0 0.0 0.39401510655123495 0.0 52-53 0.0 0.0 0.0 0.4661879245109595 0.0 54-55 0.0 0.0 0.0 0.5449881435374573 0.0 56-57 0.0 0.0 0.0 0.6421293097022349 0.0 58-59 0.0 0.0 0.0 0.7505356565386527 0.0 60-61 0.0 0.0 0.0 0.8673628660413091 0.0 62-63 0.0 0.0 0.0 0.9899067343922603 0.0 64-65 0.0 0.0 0.0 1.1196804948160555 0.0 66-67 0.0 0.0 0.0 1.264992918628966 0.0 68-69 0.0 0.0 0.0 1.4349093937595383 0.0 70-71 0.0 0.0 0.0 1.619131527429594 0.0 72-73 0.0 0.0 0.0 1.8116203981596843 0.0 74-75 0.0 0.0 0.0 2.01042841874414 0.0 76-77 0.0 0.0 0.0 2.2223371158559377 0.0 78-79 0.0 0.0 0.0 2.4673127612078742 0.0 80-81 0.0 0.0 0.0 2.736808389364997 0.0 82-83 0.0 0.0 0.0 3.020175322080483 0.0 84-85 0.0 0.0 0.0 3.3034862091209853 0.0 86-87 0.0 0.0 0.0 3.6097898343233803 0.0 88 0.0 0.0 0.0 3.8574836949120055 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4635 0.0 53.39555 1 GTATCAA 8100 0.0 42.310184 1 ATCAACG 9385 0.0 35.65686 3 TCAACGC 9475 0.0 35.31767 4 CAACGCA 9650 0.0 34.62897 5 AACGCAG 9995 0.0 33.433678 6 TATCAAC 10215 0.0 33.073097 2 ACGCAGA 11580 0.0 28.695131 7 CGCAGAG 11870 0.0 28.07326 8 GTACATG 8680 0.0 27.752155 1 TACATGG 8785 0.0 26.71048 2 TATACCG 180 7.508788E-9 26.11108 5 ACATGGG 9055 0.0 25.070099 3 GCAGAGT 13685 0.0 23.971869 9 GAGTACT 9530 0.0 21.131231 12-13 CAGAGTA 13355 0.0 20.692913 10-11 AGAGTAC 12300 0.0 20.003214 10-11 CATGGGG 6760 0.0 19.884312 4 GTGGTAT 2260 0.0 19.60717 1 TAGACCG 145 6.60368E-4 19.448252 5 >>END_MODULE