FastQCFastQC Report
Wed 25 May 2016
SRR1033024_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033024_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3290056
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40850.036.4843031
TCAACGC42350.033.0832564
ATCAACG42950.032.6210943
CAACGCA44750.031.2038975
GGTATCA30050.030.6655271
AACGCAG46650.030.228456
TATCAAC49650.029.1655562
GTACATG108750.026.8040981
TACATGG109900.025.582462
TAGACCG1551.5436544E-624.2663085
ACATGGG110000.024.1491153
ACGCAGA58100.024.1101347
CGCAGAG61600.022.5284528
GAGTACA70450.021.3554041
GTATAGA11050.020.4229181
CATGGGG88900.019.8852714
GCAGAGT72950.018.1875159
AGTACAT77000.018.1344572
TAGGTCG1851.6465732E-417.7898275
TATACCG1750.002324566516.1197625
Produced by FastQC (version 0.11.5)