##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033024_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3290056 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.614787103927714 33.0 31.0 34.0 30.0 34.0 2 31.662539786556824 33.0 31.0 34.0 30.0 34.0 3 31.72328464925825 34.0 31.0 34.0 30.0 34.0 4 35.10598603792762 37.0 35.0 37.0 33.0 37.0 5 35.15151839360789 37.0 35.0 37.0 33.0 37.0 6 35.21219061316889 37.0 35.0 37.0 33.0 37.0 7 35.10428120372419 37.0 35.0 37.0 33.0 37.0 8 35.107629171053624 37.0 35.0 37.0 33.0 37.0 9 36.73021614221764 39.0 37.0 39.0 33.0 39.0 10-11 36.72499936171299 39.0 37.5 39.0 33.0 39.0 12-13 36.68699089012466 39.0 37.0 39.0 33.0 39.0 14-15 38.07581360317271 41.0 38.0 41.0 33.0 41.0 16-17 38.08888997634083 41.0 38.5 41.0 33.0 41.0 18-19 38.0820919157607 41.0 38.5 41.0 33.0 41.0 20-21 38.072763199167426 40.0 39.0 41.0 33.0 41.0 22-23 38.044333287944035 40.0 38.5 41.0 33.0 41.0 24-25 38.043934510537206 40.0 38.0 41.0 33.0 41.0 26-27 37.943289871053864 40.0 38.0 41.0 33.0 41.0 28-29 37.94522707212278 40.0 38.0 41.0 33.0 41.0 30-31 38.09612769509091 40.0 38.0 41.0 33.0 41.0 32-33 38.08407197324301 40.0 38.0 41.0 33.0 41.0 34-35 38.035197425210995 40.0 38.0 41.0 33.0 41.0 36-37 37.97188695268409 40.0 38.0 41.0 33.0 41.0 38-39 37.8780336869646 40.0 38.0 41.0 33.0 41.0 40-41 37.79138105855949 40.0 38.0 41.0 33.0 41.0 42-43 37.667625110332466 40.0 38.0 41.0 32.0 41.0 44-45 37.536011697065334 40.0 38.0 41.0 32.0 41.0 46-47 37.39870734723056 40.0 37.5 41.0 31.5 41.0 48-49 37.270984597222665 40.0 37.0 41.0 31.0 41.0 50-51 37.12309516920077 40.0 37.0 41.0 31.0 41.0 52-53 36.95136450564975 40.0 36.5 41.0 31.0 41.0 54-55 36.77506385909541 40.0 36.0 41.0 30.5 41.0 56-57 36.57456271261036 39.5 35.5 41.0 30.0 41.0 58-59 36.36305840994804 39.0 35.0 41.0 30.0 41.0 60-61 36.11963580559115 39.0 35.0 41.0 30.0 41.0 62-63 35.848549842312714 38.5 35.0 41.0 30.0 41.0 64-65 35.55202571020068 38.0 35.0 40.0 29.0 41.0 66-67 35.233292837568726 37.0 35.0 40.0 29.0 41.0 68-69 34.901381313874296 37.0 34.0 40.0 29.0 41.0 70-71 34.55225032643821 36.0 34.0 39.0 28.5 41.0 72-73 34.19835528635379 36.0 34.0 39.0 28.0 41.0 74-75 33.837161586307346 35.0 34.0 38.5 28.0 40.0 76-77 33.47770189929898 35.0 34.0 37.0 27.0 39.5 78-79 33.10490946050766 35.0 33.5 37.0 27.0 39.0 80-81 32.7809681658914 35.0 33.0 36.5 26.0 39.0 82-83 32.586961437738445 35.0 33.0 36.0 26.5 37.5 84-85 32.39242918661567 35.0 33.0 36.0 26.5 37.0 86-87 32.24773104166008 35.0 33.0 35.0 27.0 37.0 88-89 32.053158061747276 35.0 33.0 35.0 26.0 36.0 90-91 31.861644908171776 35.0 33.0 35.0 26.0 36.0 92-93 31.65930640694262 35.0 33.0 35.0 25.5 36.0 94-95 31.469688205915038 35.0 33.0 35.0 25.0 36.0 96-97 31.305196932818166 35.0 33.0 35.0 25.0 35.0 98-99 31.137574254055252 35.0 33.0 35.0 24.0 35.0 100 30.998984211818886 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.011745195850899393 1101 2 0.0595013775029507 1101 3 0.059845712510998794 1101 4 -0.12517985747759042 1101 5 -0.0340635997623977 1101 6 0.04487776168002 1101 7 0.13430621446463675 1101 8 0.054312803867169634 1101 9 0.1639709411006649 1101 10-11 0.15133733590016618 1101 12-13 0.18555311432827182 1101 14-15 0.2488388890983657 1101 16-17 0.32610567139236224 1101 18-19 0.29129346658744026 1101 20-21 0.2372672280053436 1101 22-23 0.25414796121287964 1101 24-25 0.1924409636158515 1101 26-27 0.10249548436920719 1101 28-29 0.12777875605976874 1101 30-31 0.18026451719452297 1101 32-33 0.1778210413588468 1101 34-35 0.2009561182337123 1101 36-37 0.1571567079857985 1101 38-39 0.14613068908271742 1101 40-41 0.15128215351901986 1101 42-43 0.16381237898706047 1101 44-45 0.1623438309454741 1101 46-47 0.14283045005028328 1101 48-49 0.12522443739276667 1101 50-51 0.08666471985515045 1101 52-53 0.08335035228324728 1101 54-55 0.02294978008098525 1101 56-57 0.10562452753128326 1101 58-59 0.159746721415992 1101 60-61 0.2065203608210524 1101 62-63 0.160145550699184 1101 64-65 0.10968875003368339 1101 66-67 0.08299800293118409 1101 68-69 0.14092546671074757 1101 70-71 0.11270373071268835 1101 72-73 0.011787958578324265 1101 74-75 0.02073104570246187 1101 76-77 0.026316378592397882 1101 78-79 0.08001386524097143 1101 80-81 -0.049316960365736406 1101 82-83 0.03643292515867813 1101 84-85 0.0855649872811739 1101 86-87 0.02795667110252964 1101 88-89 0.012617324997627577 1101 90-91 0.03446717469240568 1101 92-93 0.11005946632602459 1101 94-95 0.13962031479964665 1101 96-97 0.08687505574498289 1101 98-99 0.06344388132201573 1101 100 0.022026553941177696 1102 1 0.08419884813009659 1102 2 0.1634653176485692 1102 3 0.1504007367072866 1102 4 -9.654267966467955E-4 1102 5 0.04040031057525795 1102 6 0.1314449993635023 1102 7 0.20600498300713355 1102 8 0.23805450623858349 1102 9 0.3019159544883223 1102 10-11 0.27576301429043326 1102 12-13 0.23359874901748157 1102 14-15 0.31451243233157555 1102 16-17 0.4126884610544508 1102 18-19 0.3688992654018577 1102 20-21 0.342537923152868 1102 22-23 0.335725864961681 1102 24-25 0.3049329608268607 1102 26-27 0.3302627645614322 1102 28-29 0.3677012035264795 1102 30-31 0.40743330541027234 1102 32-33 0.395257012566951 1102 34-35 0.414775217618633 1102 36-37 0.4241467262075318 1102 38-39 0.4374927313685433 1102 40-41 0.36950544364377436 1102 42-43 0.4671044736571375 1102 44-45 0.44186589272311494 1102 46-47 0.43876464444483076 1102 48-49 0.3976477985382232 1102 50-51 0.47327990947264453 1102 52-53 0.43665644373406565 1102 54-55 0.39507091444229303 1102 56-57 0.4085962514421908 1102 58-59 0.4386876575932561 1102 60-61 0.4460877047763816 1102 62-63 0.3596846937333211 1102 64-65 0.3766444243026754 1102 66-67 0.412768637166792 1102 68-69 0.42823894016741093 1102 70-71 0.38808663338581795 1102 72-73 0.44135823148963027 1102 74-75 0.3771034689340098 1102 76-77 0.3021754310103404 1102 78-79 0.26214762262491575 1102 80-81 0.4143935343686209 1102 82-83 0.3201728131325936 1102 84-85 0.3236072005350863 1102 86-87 0.28045133806986655 1102 88-89 0.27849161835460023 1102 90-91 0.3357645985071507 1102 92-93 0.3653623022293253 1102 94-95 0.4252101106280861 1102 96-97 0.35546718696220836 1102 98-99 0.3669524076942814 1102 100 0.3241921669612964 1103 1 0.11531217348914424 1103 2 0.19835807498819236 1103 3 0.13614212701062556 1103 4 0.13332123004055063 1103 5 0.09976973365645136 1103 6 0.18782084900010432 1103 7 0.2351273304291155 1103 8 0.19168556116564872 1103 9 0.3125356172593641 1103 10-11 0.3207398756488331 1103 12-13 0.3308895368733289 1103 14-15 0.40854994637945197 1103 16-17 0.4094652840624775 1103 18-19 0.4214867313122923 1103 20-21 0.36634208588911576 1103 22-23 0.4034773635063331 1103 24-25 0.42354421344536775 1103 26-27 0.40703448083547755 1103 28-29 0.3808667277858433 1103 30-31 0.41943682327174514 1103 32-33 0.3757935986504535 1103 34-35 0.3913635103811046 1103 36-37 0.4796829569640835 1103 38-39 0.5416162445122765 1103 40-41 0.49902104449166984 1103 42-43 0.4733248147660234 1103 44-45 0.5212672777750953 1103 46-47 0.562592545514832 1103 48-49 0.49988069802358837 1103 50-51 0.5532497179027942 1103 52-53 0.5642787628545705 1103 54-55 0.48916927043801195 1103 56-57 0.5813834684492107 1103 58-59 0.5610994872627302 1103 60-61 0.4987764825040415 1103 62-63 0.575172285840928 1103 64-65 0.5555099763149371 1103 66-67 0.5486161642533034 1103 68-69 0.5346696847007948 1103 70-71 0.5065496710297737 1103 72-73 0.5160603989341439 1103 74-75 0.4892061292481813 1103 76-77 0.4636028579121074 1103 78-79 0.4593480701623207 1103 80-81 0.505365063079914 1103 82-83 0.48989653832547475 1103 84-85 0.490959785357866 1103 86-87 0.47480794818594063 1103 88-89 0.5207948251990118 1103 90-91 0.5359430848442663 1103 92-93 0.5945428734005596 1103 94-95 0.500926219107285 1103 96-97 0.5264144088166418 1103 98-99 0.5354399940667847 1103 100 0.4966451272836174 1104 1 -0.03371244203552948 1104 2 0.09476806898985757 1104 3 0.1372648823382896 1104 4 0.022545209430745672 1104 5 0.024147869660005483 1104 6 0.0044239864079997915 1104 7 0.10794019900786367 1104 8 0.07867782354783515 1104 9 0.13877523450752705 1104 10-11 0.1391067924704359 1104 12-13 0.1768222492737337 1104 14-15 0.17820783205780089 1104 16-17 0.14784607443942122 1104 18-19 0.1888352988253672 1104 20-21 0.2534907396933477 1104 22-23 0.2139201391097174 1104 24-25 0.16100491998167143 1104 26-27 0.09618985823419024 1104 28-29 0.10057068186802098 1104 30-31 0.1194306066175912 1104 32-33 0.13178283599065566 1104 34-35 0.2086270305455855 1104 36-37 0.2334893496156596 1104 38-39 0.20623020241816192 1104 40-41 0.25240054754080177 1104 42-43 0.2640268120052909 1104 44-45 0.24921231779593 1104 46-47 0.23633832602622107 1104 48-49 0.22147502390687634 1104 50-51 0.17999445375944134 1104 52-53 0.16821220688905925 1104 54-55 0.18024831464860114 1104 56-57 0.1978951492189296 1104 58-59 0.19903940888912786 1104 60-61 0.26908842488693097 1104 62-63 0.310782051381743 1104 64-65 0.2353561400471449 1104 66-67 0.22512210115802134 1104 68-69 0.22625571679690637 1104 70-71 0.1559729467441926 1104 72-73 0.20590284266236836 1104 74-75 0.23066801614017862 1104 76-77 0.2014397505058696 1104 78-79 0.22312995721033246 1104 80-81 0.21326341493406886 1104 82-83 0.19769238911073472 1104 84-85 0.2249124819945152 1104 86-87 0.21868077437252254 1104 88-89 0.23178369129984588 1104 90-91 0.2630528957376477 1104 92-93 0.2617675882299828 1104 94-95 0.31155979890455754 1104 96-97 0.3570897063111289 1104 98-99 0.3961674871983796 1104 100 0.44989622098905 1105 1 -0.08079821732568604 1105 2 -0.050613286949911185 1105 3 -0.028342866501148478 1105 4 -0.00895819855089286 1105 5 -0.05373780863931188 1105 6 0.038350960092088826 1105 7 0.01863948295050477 1105 8 0.1052674099958324 1105 9 0.08470819905426907 1105 10-11 0.15210621181100237 1105 12-13 0.17758010179088046 1105 14-15 0.21207032522585934 1105 16-17 0.17047092380632733 1105 18-19 0.18363119221329072 1105 20-21 0.15318903158684805 1105 22-23 0.17377377525729543 1105 24-25 0.1777024498043005 1105 26-27 0.1456042528387016 1105 28-29 0.08290085549982962 1105 30-31 0.1209195954256117 1105 32-33 0.09851178248839432 1105 34-35 0.08802761931139713 1105 36-37 0.10857253372201825 1105 38-39 0.13731974770931998 1105 40-41 0.1869386956336001 1105 42-43 0.21985239116040844 1105 44-45 0.1652699125338586 1105 46-47 0.13358568873071164 1105 48-49 0.1535256391255473 1105 50-51 0.11779771752256352 1105 52-53 0.16474492281642483 1105 54-55 0.12731614966919125 1105 56-57 0.10160766012638334 1105 58-59 0.1406095266405103 1105 60-61 0.15867381048175133 1105 62-63 0.12961385280620874 1105 64-65 0.08295395178957676 1105 66-67 0.038271113510248256 1105 68-69 0.16168424953014693 1105 70-71 0.14751218348414596 1105 72-73 0.16596773117557717 1105 74-75 0.18946639482904715 1105 76-77 0.11971782745182225 1105 78-79 0.18455956055672829 1105 80-81 0.2594493421920063 1105 82-83 0.19517023897099506 1105 84-85 0.18947870868488081 1105 86-87 0.2119091372646693 1105 88-89 0.2697474488306071 1105 90-91 0.22035206336984814 1105 92-93 0.21660716677693514 1105 94-95 0.23968951218699885 1105 96-97 0.2826112061551598 1105 98-99 0.21404489217209033 1105 100 0.29052676678213274 1106 1 -0.07578841185716456 1106 2 -0.004476866960281711 1106 3 -0.01197224233236227 1106 4 -0.04519147102269727 1106 5 -0.034168197326579275 1106 6 0.027519862105030768 1106 7 -0.0059418122729937295 1106 8 0.01696371848112932 1106 9 0.060341046246747965 1106 10-11 0.07622559808634577 1106 12-13 0.09957684832891545 1106 14-15 0.07217615666196053 1106 16-17 0.07166486169752062 1106 18-19 0.15725504542430002 1106 20-21 0.1310271699754466 1106 22-23 0.10002425426007022 1106 24-25 0.08678678434735332 1106 26-27 0.043046002344667045 1106 28-29 0.08371797787658863 1106 30-31 0.09306135770498969 1106 32-33 0.08643152040812652 1106 34-35 0.11157201441542242 1106 36-37 0.10753845126591699 1106 38-39 0.2132116320874502 1106 40-41 0.24647028727528664 1106 42-43 0.21339242827842497 1106 44-45 0.21331764750820525 1106 46-47 0.16307911042596857 1106 48-49 0.20514241436617198 1106 50-51 0.20925907045180736 1106 52-53 0.16556110597205986 1106 54-55 0.18719713156851725 1106 56-57 0.19067681685609017 1106 58-59 0.20664804222031563 1106 60-61 0.20546298915166972 1106 62-63 0.16382216453745713 1106 64-65 0.147020103011684 1106 66-67 0.14262045868528617 1106 68-69 0.11972360665208015 1106 70-71 0.07175742538994001 1106 72-73 0.14865037211509957 1106 74-75 0.1300268468573833 1106 76-77 0.1203367741716761 1106 78-79 0.13780888995285068 1106 80-81 0.1940269556687042 1106 82-83 0.07685373648018157 1106 84-85 0.10315113841235757 1106 86-87 0.1414562824081429 1106 88-89 0.18027067013364473 1106 90-91 0.16089654863910496 1106 92-93 0.24771510958212772 1106 94-95 0.25579903288367944 1106 96-97 0.22346093828929625 1106 98-99 0.1462453369872101 1106 100 0.143893686662814 1107 1 0.10918066875619914 1107 2 0.13448138678926114 1107 3 0.12858833969827543 1107 4 0.1258563297891726 1107 5 0.11901790413538293 1107 6 0.11328232808528327 1107 7 0.09540309805558422 1107 8 0.11886788299872819 1107 9 0.17344837895480936 1107 10-11 0.14991449074076257 1107 12-13 0.12800479780948848 1107 14-15 0.13851918670734875 1107 16-17 0.1592064744206212 1107 18-19 0.18310565680122437 1107 20-21 0.14672722492232992 1107 22-23 0.22416561637783872 1107 24-25 0.16404332235705965 1107 26-27 0.2595171963566969 1107 28-29 0.2104203897423389 1107 30-31 0.22320098421484857 1107 32-33 0.2220154214185257 1107 34-35 0.21198384350238797 1107 36-37 0.21977593623480374 1107 38-39 0.28513735669012163 1107 40-41 0.24424640029640443 1107 42-43 0.25573589779975237 1107 44-45 0.32768333310799846 1107 46-47 0.2990366526902193 1107 48-49 0.319519062091274 1107 50-51 0.2919224754836307 1107 52-53 0.31749120799290864 1107 54-55 0.24893563545765574 1107 56-57 0.34072007080133915 1107 58-59 0.29650315884493494 1107 60-61 0.30964585127464517 1107 62-63 0.2737823144250129 1107 64-65 0.2476397170160567 1107 66-67 0.23669289690472795 1107 68-69 0.20667239754823896 1107 70-71 0.21490513092421537 1107 72-73 0.22708889973692692 1107 74-75 0.16785429235270755 1107 76-77 0.23988098014964265 1107 78-79 0.2772541221839191 1107 80-81 0.21819460211403197 1107 82-83 0.23811712823358988 1107 84-85 0.21336849568053395 1107 86-87 0.16125982625887758 1107 88-89 0.18037310716893273 1107 90-91 0.2491574501940086 1107 92-93 0.19387367121256105 1107 94-95 0.27037215657658464 1107 96-97 0.2598196447931933 1107 98-99 0.26160492194752294 1107 100 0.2578579231315885 1108 1 0.06332700917315037 1108 2 0.08036958490744794 1108 3 0.12244505697855601 1108 4 0.15954034009209295 1108 5 0.07802717018793714 1108 6 0.1258488487318843 1108 7 0.19626813461780301 1108 8 0.12545809293295207 1108 9 0.1050656548011446 1108 10-11 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2214 74-75 -0.17521469490672104 2214 76-77 -0.1851741237866804 2214 78-79 -0.13030981043419843 2214 80-81 -0.13821953705832613 2214 82-83 -0.14877915536008146 2214 84-85 -0.20234184478472628 2214 86-87 -0.18415578881191408 2214 88-89 -0.238961474985512 2214 90-91 -0.22055064950633962 2214 92-93 -0.20153099635533422 2214 94-95 -0.24079367120988948 2214 96-97 -0.27869842522039434 2214 98-99 -0.2703665274372611 2214 100 -0.20768731217963676 2215 1 -0.23771565045827003 2215 2 -0.32170194822424136 2215 3 -0.3731168038008974 2215 4 -0.2981203956578824 2215 5 -0.24925905280620242 2215 6 -0.19976919347776345 2215 7 -0.19344520549992694 2215 8 -0.24575096647977546 2215 9 -0.37424794773749426 2215 10-11 -0.3721869772160744 2215 12-13 -0.3634608242120052 2215 14-15 -0.5209358273787501 2215 16-17 -0.49996408385151625 2215 18-19 -0.4933419277968625 2215 20-21 -0.44075726870963905 2215 22-23 -0.4504042516219968 2215 24-25 -0.36917329715268465 2215 26-27 -0.3997284585183465 2215 28-29 -0.45556298662035744 2215 30-31 -0.4223646091248625 2215 32-33 -0.36999703051287014 2215 34-35 -0.38000584325315145 2215 36-37 -0.3570575373581448 2215 38-39 -0.4114127827637546 2215 40-41 -0.3975172411614878 2215 42-43 -0.38039117657429955 2215 44-45 -0.29469972282018375 2215 46-47 -0.36030845534821054 2215 48-49 -0.28874370505990044 2215 50-51 -0.3491823494906967 2215 52-53 -0.35673880725657625 2215 54-55 -0.35742155748861393 2215 56-57 -0.40510719307479093 2215 58-59 -0.39082356659039164 2215 60-61 -0.4632536458176659 2215 62-63 -0.5447133506935202 2215 64-65 -0.6726975117923217 2215 66-67 -0.5953239928120837 2215 68-69 -0.4693316597790016 2215 70-71 -0.4649001579739078 2215 72-73 -0.5686149380227761 2215 74-75 -0.4682165977683468 2215 76-77 -0.4309388988642553 2215 78-79 -0.5123376260143999 2215 80-81 -0.5922957952868799 2215 82-83 -0.46558884807903667 2215 84-85 -0.4756725027762947 2215 86-87 -0.4501235958887939 2215 88-89 -0.4821614874389013 2215 90-91 -0.47057956528841416 2215 92-93 -0.49625598509090807 2215 94-95 -0.5330655256893024 2215 96-97 -0.4874075182595057 2215 98-99 -0.4563024032495129 2215 100 -0.3983254721870111 2216 1 -0.5614117609411231 2216 2 -0.6340857257861536 2216 3 -0.556598583599591 2216 4 -0.6722017142726671 2216 5 -0.5341804684843368 2216 6 -0.6753861063881814 2216 7 -0.6762681538173183 2216 8 -0.5522313608637788 2216 9 -0.6851812676660884 2216 10-11 -0.6863576825007911 2216 12-13 -0.8228486528786121 2216 14-15 -0.9766472174393286 2216 16-17 -0.9882970126070347 2216 18-19 -1.0527259877200947 2216 20-21 -0.8962887735439793 2216 22-23 -0.8721967639372608 2216 24-25 -0.9777320437650019 2216 26-27 -1.0378499722801635 2216 28-29 -0.9603328559881632 2216 30-31 -1.0688215366573601 2216 32-33 -1.1043530182497747 2216 34-35 -1.24001466607411 2216 36-37 -1.2665953435421144 2216 38-39 -1.1840383519798436 2216 40-41 -1.1278681092960738 2216 42-43 -1.1595720439489767 2216 44-45 -1.138510992503143 2216 46-47 -1.0897861554286692 2216 48-49 -1.117190667210508 2216 50-51 -1.0910266001214453 2216 52-53 -1.1367986376636594 2216 54-55 -1.1611443719808179 2216 56-57 -1.1665428198463914 2216 58-59 -1.1020365925880569 2216 60-61 -1.0870423715629727 2216 62-63 -1.1155756699594548 2216 64-65 -1.1572045102390263 2216 66-67 -1.1495316114392438 2216 68-69 -1.1522856549052776 2216 70-71 -1.118560363949122 2216 72-73 -1.0995326058890527 2216 74-75 -1.1835896553163892 2216 76-77 -1.0824826529710947 2216 78-79 -1.0671376038409122 2216 80-81 -1.0908147701469204 2216 82-83 -1.2330062575267462 2216 84-85 -1.0872053446579955 2216 86-87 -1.04634300024372 2216 88-89 -1.1112226664297715 2216 90-91 -1.1495468696462687 2216 92-93 -1.3849471398635913 2216 94-95 -1.3861198258669845 2216 96-97 -1.3219713409515848 2216 98-99 -1.19046761173637 2216 100 -1.135200535640685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40202.0 3 8038.0 4 4624.0 5 3920.0 6 3279.0 7 3488.0 8 3943.0 9 4437.0 10 4987.0 11 5398.0 12 6104.0 13 6721.0 14 7336.0 15 7776.0 16 7859.0 17 7974.0 18 7607.0 19 7410.0 20 7231.0 21 7505.0 22 9020.0 23 12364.0 24 19128.0 25 24093.0 26 23609.0 27 21672.0 28 25283.0 29 31484.0 30 38329.0 31 47457.0 32 61864.0 33 83710.0 34 121191.0 35 190221.0 36 353837.0 37 708560.0 38 1007246.0 39 354672.0 40 477.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.97406529720471 18.785628435110326 12.952327541951227 24.287978725733737 2 15.65978866402104 19.671438262631067 39.82878325367159 24.839989819676305 3 18.582725153319753 24.89202384507634 29.559799113564576 26.965451888039325 4 12.61547483727595 16.27909664769753 37.53717353673066 33.56825497829586 5 13.683456410802425 37.83130147769015 34.867129569773894 13.61811254173353 6 32.209659779716986 37.04873697003622 17.450758791470385 13.290844458776412 7 28.35503551182602 32.38390738997662 21.654425790231677 17.606631307965685 8 26.062664869519182 36.36932597841597 19.621655830029606 17.94635332203524 9 25.698408666008493 16.877997727317673 19.877451614228146 37.54614199244569 10-11 24.767210906127975 26.69848411733958 28.053778509894222 20.48052646663823 12-13 25.106118327659438 23.887908375471067 28.15678538866137 22.849187908208123 14-15 22.365328907623137 25.627256638299055 25.01701250261102 26.990401951466787 16-17 22.65970039432046 29.079624383413265 25.63402370972101 22.62665151254527 18-19 22.699787621566912 28.138187607693325 27.072385890137323 22.08963888060243 20-21 23.57255528902099 27.32975970818775 26.822160937863643 22.27552406492762 22-23 23.234843887352497 27.00915839171531 26.751387124787477 23.004610596144715 24-25 23.147976154247687 27.04928424133108 26.851005394145332 22.951734210275905 26-27 22.991970408866376 27.243046841768248 26.706604019758686 23.058378729606694 28-29 22.955467557193863 27.268624123311252 26.818646325662876 22.957261993832002 30-31 23.122974823746453 27.52553024351925 26.55193994869997 22.79955498403433 32-33 22.953099645194307 27.216136435183707 26.889085580714873 22.941678338907113 34-35 22.934564051178437 27.143657994852482 26.951872616933294 22.969905337035783 36-37 23.16428963618562 27.221478257154164 26.59786033044619 23.016371776214022 38-39 23.142734602065072 27.408605535758557 26.620750805694843 22.827909056481527 40-41 22.976028891145333 27.27038359493018 26.841804431135614 22.911783082788876 42-43 22.89023503682051 27.196668055737216 26.812204759138208 23.100892148304066 44-45 22.9469410151893 27.245674180560357 26.84128112450166 22.966103679748684 46-47 23.022782199495957 27.26087359902173 26.829321340193797 22.88702286128851 48-49 23.09601143223547 27.189383813945113 26.711337271022217 23.0032674827972 50-51 22.947370886392434 27.350133193985073 26.770074757747548 22.932421161874945 52-53 23.042058685845152 27.18353326298762 26.788986890937476 22.98542116022975 54-55 23.07080030646139 27.36360171820334 26.689544818457517 22.876053156877756 56-57 22.770224759513205 27.252503491360454 26.906447051996064 23.07082469713028 58-59 22.84241464697625 27.04330887618525 26.973628283082046 23.140648193756455 60-61 22.90689839281877 27.35549062846387 26.745310126046185 22.99230085267118 62-63 22.907208809264656 27.294458272836756 26.700049471966974 23.09828344593161 64-65 22.93948317003361 27.20890314870978 26.74524437626117 23.106369304995436 66-67 22.827019914763984 27.21052785256578 26.76729995510692 23.195152277563317 68-69 22.919595714922476 27.17705622419174 26.888081297427767 23.01526676345801 70-71 22.807893646382585 27.13066486217292 26.896866339475455 23.16457515196904 72-73 22.83745548166478 27.251128801441194 26.837730411514332 23.073685305379698 74-75 22.895966657360216 27.334079065900006 26.670340585673785 23.09961369106599 76-77 22.94629780111234 27.232963973081997 26.694859327633775 23.12587889817189 78-79 23.01295394024489 27.24239802001305 26.78206960456369 22.962578435178372 80-81 22.96200046191266 27.118484549416976 26.938174405021375 22.98134058364899 82-83 22.838737086157966 27.2092355638056 26.97563688824608 22.976390461790356 84-85 22.84602443728339 27.17271441158114 26.712962646214333 23.268298504921134 86-87 22.90014291361328 27.22667011281053 26.833475233374887 23.039711740201298 88-89 22.743699106059175 27.099936888814696 26.887721537212716 23.268642467913413 90-91 22.753623518912537 27.156578130190507 26.89669274794508 23.193105602951874 92-93 22.9658301425979 27.127945855611724 26.917147506847716 22.989076494942665 94-95 22.795040924007612 27.028727763938377 26.922774607426753 23.253456704627258 96-97 22.88538447223327 26.991296023976076 26.95425912208555 23.16906038170511 98-99 22.68253827314862 27.18237376535533 26.90427653364747 23.230811427848586 100 22.929206459745195 26.908235551710348 26.76512491770226 23.397433070842197 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 769.0 1 702.5 2 598.5 3 524.0 4 486.5 5 499.0 6 489.5 7 453.0 8 403.5 9 387.0 10 374.5 11 352.0 12 351.0 13 347.0 14 384.0 15 440.5 16 546.0 17 699.0 18 906.5 19 1244.0 20 1761.0 21 2408.5 22 3430.5 23 5305.0 24 7957.0 25 11150.0 26 15626.0 27 21304.5 28 27892.5 29 35031.0 30 41910.5 31 48692.5 32 55842.0 33 63827.5 34 71430.0 35 77177.0 36 82079.5 37 89334.0 38 95836.5 39 99353.0 40 103089.0 41 106992.0 42 110143.0 43 112249.5 44 113258.0 45 114940.5 46 114090.0 47 109635.5 48 109839.0 49 111133.5 50 108026.0 51 105721.5 52 103707.5 53 103484.5 54 103005.0 55 98238.5 56 90513.0 57 83863.0 58 76845.5 59 67443.0 60 60596.0 61 54939.0 62 49021.5 63 42172.0 64 35067.5 65 29530.5 66 24060.5 67 18724.0 68 14994.0 69 12150.0 70 9721.0 71 7218.5 72 5335.0 73 4046.0 74 2948.0 75 1941.5 76 1152.0 77 760.5 78 522.5 79 335.0 80 214.0 81 130.0 82 75.5 83 55.0 84 36.0 85 29.0 86 23.5 87 17.5 88 14.0 89 15.0 90 14.0 91 8.5 92 5.0 93 3.5 94 4.0 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 11.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.059330297113483775 2 0.04130628779570925 3 0.055622153543891044 4 0.07401089829474027 5 0.08604716758620522 6 0.08878268333426544 7 0.0906367551190618 8 0.10230828897745206 9 0.09698923057844608 10-11 0.10303775984360143 12-13 0.10676110072290564 14-15 0.10645715452867671 16-17 0.07603214048636253 18-19 0.09831139652334185 20-21 0.09151819908232565 22-23 0.05682274101109525 24-25 0.02276556994774557 26-27 0.17717023661603326 28-29 0.06431501469883796 30-31 0.07700476830789507 32-33 0.0711538040689885 34-35 0.02133702283486968 36-37 0.05302341358323384 38-39 0.07662483556510892 40-41 0.08151836929219441 42-43 0.07556102388530772 44-45 0.07334221666743666 46-47 0.07966429750739805 48-49 0.07629049475145712 50-51 0.07203524803225234 52-53 0.07546984002703906 54-55 0.0669593465886295 56-57 0.06983163812409272 58-59 0.0667617815623807 60-61 0.06366153038124579 62-63 0.0709866336621626 64-65 0.0664274407487289 66-67 0.06820552598496804 68-69 0.06770401476449033 70-71 0.059831808333961484 72-73 0.0591023374678121 74-75 0.06121476351770304 76-77 0.05616925669350308 78-79 0.053236175919194086 80-81 0.04727883051230739 82-83 0.052400323885064576 84-85 0.043570686942714654 86-87 0.04121510393744058 88-89 0.04302358379310261 90-91 0.03946741332062433 92-93 0.04157983937051527 94-95 0.04068319809753999 96-97 0.04388983044665501 98-99 0.04431535511857549 100 0.05139730144410916 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3290056.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.578209519321426 #Duplication Level Percentage of deduplicated Percentage of total 1 71.92930189250217 37.81913905482502 2 14.558847028485292 15.309562188468998 3 5.388919946502562 8.500192860901864 4 2.6657369863702605 5.606387111711202 5 1.4636837087467018 3.847893435425076 6 0.920352927032465 2.9034305417540223 7 0.6207059830232633 2.2844926457705457 8 0.44195335457791146 1.8589692859811504 9 0.318210113334037 1.5057826209039613 >10 1.5869006509194747 14.44955863844756 >50 0.07567517328720129 2.7030696552958697 >100 0.028151383033277518 2.5867503519909167 >500 0.0012139961460478602 0.41310002176322147 >1k 3.468560393193804E-4 0.2116715867605551 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.5197309711445641E-5 0.0 20-21 0.0 0.0 0.0 7.59865485572282E-5 0.0 22-23 0.0 0.0 0.0 1.0638116798011949E-4 0.0 24-25 0.0 0.0 0.0 1.823677165373477E-4 0.0 26-27 0.0 0.0 0.0 3.1914350394035844E-4 0.0 28-29 0.0 0.0 0.0 9.878251312439666E-4 0.0 30-31 0.0 0.0 0.0 0.0038297220472843017 0.0 32-33 0.0 0.0 0.0 0.010197394816380025 0.0 34-35 0.0 0.0 0.0 0.01892065059074982 0.0 36-37 0.0 0.0 0.0 0.03594163746756894 0.0 38-39 0.0 0.0 0.0 0.06487731515816145 0.0 40-41 0.0 0.0 0.0 0.09853935616901355 0.0 42-43 0.0 0.0 0.0 0.13299165728486081 0.0 44-45 0.0 0.0 0.0 0.17355327690470923 0.0 46-47 0.0 0.0 0.0 0.2260903765771768 0.0 48-49 0.0 0.0 0.0 0.2830498933756751 0.0 50-51 0.0 0.0 0.0 0.34523728471491066 0.0 52-53 0.0 0.0 0.0 0.4087164473796191 0.0 54-55 0.0 0.0 0.0 0.479338345608707 0.0 56-57 0.0 0.0 0.0 0.5618445400321453 0.0 58-59 0.0 0.0 0.0 0.6603990935108703 0.0 60-61 0.0 0.0 0.0 0.7634520506641832 0.0 62-63 0.0 0.0 0.0 0.8720976177913081 0.0 64-65 0.0 0.0 0.0 0.9851656020444637 0.0 66-67 0.0 0.0 0.0 1.1194642279645088 0.0 68-69 0.0 0.0 0.0 1.2723643609713635 0.0 70-71 0.0 0.0 0.0 1.4399754897788974 0.0 72-73 0.0 0.0 0.0 1.61535244384898 0.0 74-75 0.0 0.0 0.0 1.791413276856078 0.0 76-77 0.0 0.0 0.0 1.9858932492334478 0.0 78-79 0.0 0.0 0.0 2.2086554149838182 0.0 80-81 0.0 0.0 0.0 2.453271312099247 0.0 82-83 0.0 0.0 0.0 2.709406769975952 0.0 84-85 0.0 0.0 0.0 2.964903940844776 0.0 86-87 0.0 0.0 0.0 3.241343004495972 0.0 88 0.0 0.0 0.0 3.4680868653907413 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4085 0.0 36.484303 1 TCAACGC 4235 0.0 33.083256 4 ATCAACG 4295 0.0 32.621094 3 CAACGCA 4475 0.0 31.203897 5 GGTATCA 3005 0.0 30.665527 1 AACGCAG 4665 0.0 30.22845 6 TATCAAC 4965 0.0 29.165556 2 GTACATG 10875 0.0 26.804098 1 TACATGG 10990 0.0 25.58246 2 TAGACCG 155 1.5436544E-6 24.266308 5 ACATGGG 11000 0.0 24.149115 3 ACGCAGA 5810 0.0 24.110134 7 CGCAGAG 6160 0.0 22.528452 8 GAGTACA 7045 0.0 21.355404 1 GTATAGA 1105 0.0 20.422918 1 CATGGGG 8890 0.0 19.885271 4 GCAGAGT 7295 0.0 18.187515 9 AGTACAT 7700 0.0 18.134457 2 TAGGTCG 185 1.6465732E-4 17.789827 5 TATACCG 175 0.0023245665 16.119762 5 >>END_MODULE