FastQCFastQC Report
Wed 25 May 2016
SRR1033024_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033024_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3290056
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59800.18175982414888986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50720.15416150971290457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46250.049.9350171
GTATCAA73550.041.3330881
TCAACGC89150.033.6349454
ATCAACG89900.033.407133
CAACGCA92800.032.3105435
TATCAAC93650.032.2432982
AACGCAG96450.031.136536
GTACATG107500.027.3149491
ACGCAGA115200.026.0687377
TACATGG111000.025.8454152
CGCAGAG117600.025.3768658
ACATGGG106350.025.2788073
GTGGTAT20200.025.1995751
CATGGGG87650.022.306574
GCAGAGT130400.022.1290329
GAGTACA67150.020.495461
GAGTACT83500.019.41813512-13
CAGAGTA129750.019.14296710-11
AGAGTAC122650.018.69923410-11
ATGGGGG40450.018.2411735
Produced by FastQC (version 0.11.5)