##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033024_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3290056 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.687170978244744 34.0 31.0 34.0 31.0 34.0 2 32.68499381165549 34.0 31.0 34.0 31.0 34.0 3 32.78507447897543 34.0 31.0 34.0 31.0 34.0 4 36.28721638780617 37.0 37.0 37.0 35.0 37.0 5 36.26661400292274 37.0 37.0 37.0 35.0 37.0 6 36.33318156286702 37.0 37.0 37.0 35.0 37.0 7 36.2825954938153 37.0 37.0 37.0 35.0 37.0 8 36.29266979042302 37.0 37.0 37.0 35.0 37.0 9 38.093597191050854 39.0 39.0 39.0 35.0 39.0 10-11 38.04996100370328 39.0 39.0 39.0 35.0 39.0 12-13 38.02296647838213 39.0 38.0 39.0 35.0 39.0 14-15 39.51994282164194 41.0 39.0 41.0 37.0 41.0 16-17 39.51197791162217 41.0 39.0 41.0 36.5 41.0 18-19 39.51782629231843 41.0 39.5 41.0 36.5 41.0 20-21 39.501681126400285 41.0 39.5 41.0 36.5 41.0 22-23 39.433921945401536 41.0 39.0 41.0 36.0 41.0 24-25 39.374592560126636 41.0 39.0 41.0 36.0 41.0 26-27 39.26064130823305 41.0 39.0 41.0 36.0 41.0 28-29 39.20707139939259 41.0 39.0 41.0 36.0 41.0 30-31 39.20955965491165 41.0 39.0 41.0 36.0 41.0 32-33 39.18119022898091 41.0 39.0 41.0 35.0 41.0 34-35 39.096171767289064 41.0 39.0 41.0 35.0 41.0 36-37 39.01666749745293 41.0 39.0 41.0 35.0 41.0 38-39 38.92754758581617 41.0 39.0 41.0 35.0 41.0 40-41 38.82295483724289 41.0 39.0 41.0 35.0 41.0 42-43 38.70105827985907 41.0 38.0 41.0 35.0 41.0 44-45 38.52924843832445 40.5 38.0 41.0 35.0 41.0 46-47 38.32308872554145 40.0 38.0 41.0 34.0 41.0 48-49 38.11171390395786 40.0 38.0 41.0 34.0 41.0 50-51 37.95227072122785 40.0 38.0 41.0 34.0 41.0 52-53 37.79815981855628 40.0 37.0 41.0 33.0 41.0 54-55 37.62828049127431 40.0 37.0 41.0 33.0 41.0 56-57 37.44927730713398 40.0 36.5 41.0 33.0 41.0 58-59 37.24701995953868 40.0 36.0 41.0 33.0 41.0 60-61 37.03181663169259 39.0 35.5 41.0 33.0 41.0 62-63 36.77700197200291 39.0 35.0 41.0 32.0 41.0 64-65 36.495131845780136 38.5 35.0 41.0 32.0 41.0 66-67 36.19983322472322 38.0 35.0 40.5 31.5 41.0 68-69 35.88443585762674 37.0 35.0 40.0 31.0 41.0 70-71 35.55316672421381 37.0 35.0 39.5 31.0 41.0 72-73 35.212356415817844 36.0 35.0 39.0 31.0 41.0 74-75 34.86929432204194 36.0 35.0 39.0 31.0 40.5 76-77 34.52498346532704 35.0 34.5 37.5 30.0 39.5 78-79 34.18319125875061 35.0 34.0 37.0 30.0 39.0 80-81 33.87961587887865 35.0 34.0 37.0 30.0 39.0 82-83 33.60920391628592 35.0 34.0 36.0 30.0 38.0 84-85 33.415376668360665 35.0 34.0 36.0 30.0 37.0 86-87 33.23223738440926 35.0 34.0 35.5 30.0 37.0 88-89 33.04973973087388 35.0 34.0 35.0 30.0 36.5 90-91 32.88624859272912 35.0 34.0 35.0 29.5 36.0 92-93 32.72380804460471 35.0 34.0 35.0 29.0 36.0 94-95 32.578195933443084 35.0 34.0 35.0 29.0 36.0 96-97 32.442899756113576 35.0 34.0 35.0 29.0 35.5 98-99 32.32521589298176 35.0 34.0 35.0 29.0 35.0 100 32.22689978529241 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0592176476798727 1101 2 0.03103403826984419 1101 3 0.0601027214739176 1101 4 0.06781411298702977 1101 5 0.06875452501386548 1101 6 0.0586635982267012 1101 7 0.15803307612550554 1101 8 0.1255034902086507 1101 9 0.17514694833923983 1101 10-11 0.20175510603643687 1101 12-13 0.19033592429164514 1101 14-15 0.2457963634688909 1101 16-17 0.2801239747899089 1101 18-19 0.2701799324947345 1101 20-21 0.27044894115211804 1101 22-23 0.27080241520029347 1101 24-25 0.33030811258600323 1101 26-27 0.32859482658581385 1101 28-29 0.3829817106974289 1101 30-31 0.37919382158423076 1101 32-33 0.30532141071163466 1101 34-35 0.35454429045545766 1101 36-37 0.3628482319386066 1101 38-39 0.29796376088903287 1101 40-41 0.32477345427634674 1101 42-43 0.3180601956497213 1101 44-45 0.35275409957756665 1101 46-47 0.36650762980583096 1101 48-49 0.3172728384859482 1101 50-51 0.308504993975923 1101 52-53 0.30998572743725106 1101 54-55 0.37127208930049704 1101 56-57 0.29847659096176926 1101 58-59 0.34094306350886683 1101 60-61 0.3361464924251365 1101 62-63 0.29161129103164285 1101 64-65 0.35699575881135104 1101 66-67 0.3966860455371375 1101 68-69 0.3442950205047737 1101 70-71 0.3245334493316534 1101 72-73 0.351597335217285 1101 74-75 0.3272964377635148 1101 76-77 0.3306938350135624 1101 78-79 0.29668359970606417 1101 80-81 0.3245780166713672 1101 82-83 0.29202166365393367 1101 84-85 0.3145358335160253 1101 86-87 0.32774245863840434 1101 88-89 0.38006058183325564 1101 90-91 0.3407199674992256 1101 92-93 0.33749947304971073 1101 94-95 0.38246070109813246 1101 96-97 0.40497992658063 1101 98-99 0.3904803525230207 1101 100 0.4021353820702558 1102 1 0.10356359625984624 1102 2 0.0815989638925032 1102 3 0.12628235201074745 1102 4 0.10768368372649917 1102 5 0.10112415407325415 1102 6 0.12114082105804158 1102 7 0.11729751937763666 1102 8 0.15996486038980606 1102 9 0.20379472971989543 1102 10-11 0.17637790575546575 1102 12-13 0.1975472471688633 1102 14-15 0.2658549166683244 1102 16-17 0.26527857441718083 1102 18-19 0.2551327451996599 1102 20-21 0.2513218218710236 1102 22-23 0.24928521600795506 1102 24-25 0.27717024170371474 1102 26-27 0.29054185654669595 1102 28-29 0.24003349619417236 1102 30-31 0.2881687388347416 1102 32-33 0.2866901403482629 1102 34-35 0.2902910316679481 1102 36-37 0.23175282832588096 1102 38-39 0.19943653326249233 1102 40-41 0.24237609085512446 1102 42-43 0.2645668324565804 1102 44-45 0.3055713405010465 1102 46-47 0.3049663768865969 1102 48-49 0.35095868333422686 1102 50-51 0.30971895550550954 1102 52-53 0.3455087793575302 1102 54-55 0.35727867815543135 1102 56-57 0.3486310739491998 1102 58-59 0.3521850704398659 1102 60-61 0.38046548424220816 1102 62-63 0.37368167410279796 1102 64-65 0.35163123339293634 1102 66-67 0.3485627805547509 1102 68-69 0.41211901357895897 1102 70-71 0.28014680640767153 1102 72-73 0.34885811338644857 1102 74-75 0.33930347244184134 1102 76-77 0.3436707947572657 1102 78-79 0.3431238814042388 1102 80-81 0.33005464593553313 1102 82-83 0.2962077378938943 1102 84-85 0.34218997306392396 1102 86-87 0.33031942149250426 1102 88-89 0.29083875100249656 1102 90-91 0.2797906849379075 1102 92-93 0.33739877398826934 1102 94-95 0.3523008785980508 1102 96-97 0.3774114525148491 1102 98-99 0.32465815829436195 1102 100 0.327472406406514 1103 1 0.10841554211749127 1103 2 0.06966476595519566 1103 3 0.08499136567051124 1103 4 0.0673517061213218 1103 5 0.09810150615687974 1103 6 0.1263578186026706 1103 7 0.11190368122070993 1103 8 0.09783824979248124 1103 9 0.11521364124248379 1103 10-11 0.1369313281025697 1103 12-13 0.1685223216690801 1103 14-15 0.22639926259369503 1103 16-17 0.2520807839772985 1103 18-19 0.21917256076876868 1103 20-21 0.2241469902897677 1103 22-23 0.2525479652012379 1103 24-25 0.252772994652247 1103 26-27 0.2012774431765294 1103 28-29 0.22975769016927217 1103 30-31 0.23606409367669556 1103 32-33 0.18921228843806404 1103 34-35 0.25314024013664493 1103 36-37 0.2686484826398825 1103 38-39 0.31405790060547645 1103 40-41 0.30498367699821216 1103 42-43 0.31099063502347235 1103 44-45 0.33929209241775027 1103 46-47 0.2677039254198874 1103 48-49 0.2167548114424278 1103 50-51 0.24993810121973326 1103 52-53 0.2321232765474548 1103 54-55 0.28355691386861537 1103 56-57 0.19605123821747128 1103 58-59 0.20605253623060804 1103 60-61 0.2725978224606038 1103 62-63 0.1507166066068848 1103 64-65 0.2722897427502531 1103 66-67 0.22335648520848395 1103 68-69 0.19762389327772212 1103 70-71 0.2387410182937515 1103 72-73 0.22153067823112593 1103 74-75 0.2381936055984326 1103 76-77 0.2828918582396014 1103 78-79 0.20527358201945844 1103 80-81 0.23350603010230486 1103 82-83 0.1419243026876984 1103 84-85 0.1910949302114986 1103 86-87 0.23558274868428697 1103 88-89 0.17991103846773626 1103 90-91 0.19550471381938905 1103 92-93 0.2956719448586398 1103 94-95 0.2450363001566913 1103 96-97 0.23505448532636564 1103 98-99 0.2857619758523029 1103 100 0.29812893341348 1104 1 0.055929905466229 1104 2 0.07288368003271728 1104 3 0.08898581567760289 1104 4 0.025467819237029232 1104 5 0.055809089490367114 1104 6 0.09287226523650105 1104 7 0.12382262917827802 1104 8 0.10503534688611182 1104 9 0.13741870226486697 1104 10-11 0.12880130480699137 1104 12-13 0.1494172639714364 1104 14-15 0.16533916181730746 1104 16-17 0.1929632568224804 1104 18-19 0.22825056436757762 1104 20-21 0.18898619845640496 1104 22-23 0.18450575797223223 1104 24-25 0.21068976578200704 1104 26-27 0.20100097567633668 1104 28-29 0.246031173675469 1104 30-31 0.1830196240517168 1104 32-33 0.2099049473780994 1104 34-35 0.17281505157153987 1104 36-37 0.18642478877819002 1104 38-39 0.1756436915505759 1104 40-41 0.17781071973869444 1104 42-43 0.19044604850921587 1104 44-45 0.16055470010868333 1104 46-47 0.12294059563152615 1104 48-49 0.040694837424908314 1104 50-51 0.13960873317949307 1104 52-53 0.07261218333954389 1104 54-55 0.11745721367115891 1104 56-57 0.1362542550213206 1104 58-59 0.18280793362534808 1104 60-61 0.2034869642964452 1104 62-63 0.13506688134542344 1104 64-65 0.07062787936453674 1104 66-67 0.10549238488319901 1104 68-69 0.12227516728039234 1104 70-71 0.16741901468181197 1104 72-73 0.13306887871474515 1104 74-75 0.050618290500395346 1104 76-77 0.1326822970635675 1104 78-79 0.05201562478538335 1104 80-81 0.06487491023555947 1104 82-83 0.0748892219703734 1104 84-85 0.12358387353297218 1104 86-87 0.0913867420973915 1104 88-89 0.09837136682027392 1104 90-91 0.15404640851926388 1104 92-93 0.1552224831414577 1104 94-95 0.1971514394409013 1104 96-97 0.24810425076063325 1104 98-99 0.18283725173356657 1104 100 0.17036893754967508 1105 1 0.024648039056827997 1105 2 0.043074576285548005 1105 3 0.032734745466200366 1105 4 0.026283545751439874 1105 5 0.02261994789320454 1105 6 0.04659619024430128 1105 7 0.0186932297947493 1105 8 0.015394796859496296 1105 9 0.03594703771317853 1105 10-11 0.041241629640602184 1105 12-13 0.06887958172749364 1105 14-15 0.1188249843172926 1105 16-17 0.14652014403795732 1105 18-19 0.07160042917499254 1105 20-21 0.10307534074993185 1105 22-23 0.15238064532663032 1105 24-25 0.08698732178073953 1105 26-27 0.1164353480365179 1105 28-29 0.10691201636014824 1105 30-31 0.10941767055768281 1105 32-33 0.12450560385179443 1105 34-35 0.13757065769374321 1105 36-37 0.1640150530192983 1105 38-39 0.17790624567716407 1105 40-41 0.13220815255262863 1105 42-43 0.08121728190519661 1105 44-45 0.0847260373162868 1105 46-47 0.20410815141062955 1105 48-49 0.24276795855328714 1105 50-51 0.19663964043842697 1105 52-53 0.2130264638476902 1105 54-55 0.20219162001027513 1105 56-57 0.1634052170108049 1105 58-59 0.17954480437452247 1105 60-61 0.2472783632874993 1105 62-63 0.22205428914207914 1105 64-65 0.20613601618518373 1105 66-67 0.1880632902387518 1105 68-69 0.2089950110665768 1105 70-71 0.19460767400310175 1105 72-73 0.16718331236913997 1105 74-75 0.1387284047413928 1105 76-77 0.153129294124021 1105 78-79 0.23090948463326555 1105 80-81 0.1892204631288692 1105 82-83 0.21152194005824754 1105 84-85 0.20064146041449504 1105 86-87 0.12423178673514457 1105 88-89 0.19996183711334936 1105 90-91 0.2183487940617681 1105 92-93 0.24036343589789055 1105 94-95 0.16635528607753258 1105 96-97 0.18102583281118712 1105 98-99 0.16411696656050623 1105 100 0.20880664584472441 1106 1 -0.023907814544898542 1106 2 -0.011990079696538203 1106 3 -0.008776949715219473 1106 4 0.013938423314570514 1106 5 0.021233657516091853 1106 6 0.02702094360483187 1106 7 0.0796674650809166 1106 8 0.02979681355509456 1106 9 0.04631603890786806 1106 10-11 0.0759054636163583 1106 12-13 0.058541574829853005 1106 14-15 0.10326960408301744 1106 16-17 0.11516330763524962 1106 18-19 0.10643707983733464 1106 20-21 0.06622687844275532 1106 22-23 0.09397611903219172 1106 24-25 0.05398872685553613 1106 26-27 0.06383332983817525 1106 28-29 0.056983021477030604 1106 30-31 -0.020636278303605593 1106 32-33 0.008882787540905213 1106 34-35 -0.01953849482825376 1106 36-37 0.05124277281582579 1106 38-39 0.05349513732109301 1106 40-41 0.04492257673494038 1106 42-43 0.07590316879291947 1106 44-45 0.030362040291748826 1106 46-47 -0.05754006895318042 1106 48-49 -0.04082704178483709 1106 50-51 -0.037281833161941336 1106 52-53 -0.04379415994541347 1106 54-55 0.005860593404015901 1106 56-57 -0.0821625985111396 1106 58-59 -0.08657035164888782 1106 60-61 -0.06158262447257101 1106 62-63 -0.07391213182434342 1106 64-65 -0.06891582882079206 1106 66-67 -0.038408281416309364 1106 68-69 -0.0782948851682761 1106 70-71 -0.031476938549403144 1106 72-73 -0.012540406345401323 1106 74-75 -0.06449274802104554 1106 76-77 -0.0811472050607378 1106 78-79 -0.05384733223364435 1106 80-81 -0.07515055926967307 1106 82-83 -0.057844799491974186 1106 84-85 -0.03092632505236992 1106 86-87 -0.10718854101546071 1106 88-89 -0.007251005420599199 1106 90-91 -0.0747464279325385 1106 92-93 -0.032593946004453755 1106 94-95 -0.048283038652535026 1106 96-97 -0.06778980935307288 1106 98-99 0.026487260123467138 1106 100 -0.06295135068391033 1107 1 -0.03177812504679167 1107 2 -0.03844967739025407 1107 3 0.009626786474356663 1107 4 0.024588159009681476 1107 5 0.006167733647991724 1107 6 0.02681421154692032 1107 7 0.024896599511500028 1107 8 2.532046103951302E-4 1107 9 0.017446879367206236 1107 10-11 0.08401303910142843 1107 12-13 0.035605588191508275 1107 14-15 0.08036297364461831 1107 16-17 0.04157796680852499 1107 18-19 0.05734645316049125 1107 20-21 0.08215546583232225 1107 22-23 0.08664530612061583 1107 24-25 0.05795856453680415 1107 26-27 0.06148235652530332 1107 28-29 0.08974150591428298 1107 30-31 0.07286588166861208 1107 32-33 0.07228902086546896 1107 34-35 0.04971079901270059 1107 36-37 0.08420353014137305 1107 38-39 0.08789156146343657 1107 40-41 0.03537239794701463 1107 42-43 0.053937856673343276 1107 44-45 0.008293052478649088 1107 46-47 -0.02354051090643594 1107 48-49 -0.09077667794136346 1107 50-51 -0.03383780589758345 1107 52-53 -0.07110075213403633 1107 54-55 -0.08454039990078854 1107 56-57 -0.07115012175456314 1107 58-59 -0.09273825028503069 1107 60-61 -0.08146680497380743 1107 62-63 -0.03261582396723384 1107 64-65 -0.05369017997445269 1107 66-67 -0.048370252131455516 1107 68-69 -0.0659359869200884 1107 70-71 -0.05828571399702298 1107 72-73 -0.09294202145662922 1107 74-75 -0.09950404210675146 1107 76-77 -0.06727838243510575 1107 78-79 -0.0330518708755676 1107 80-81 -0.06401551728163923 1107 82-83 -0.051708610506423724 1107 84-85 -0.042313867519773396 1107 86-87 -0.039471653198752676 1107 88-89 -0.056180544595143544 1107 90-91 -0.08402329678290243 1107 92-93 -0.028139854889744242 1107 94-95 -0.018702765424350787 1107 96-97 0.005766794602806158 1107 98-99 0.022405138074013564 1107 100 -0.0063860409425586795 1108 1 0.021672582099327542 1108 2 0.0742775225285115 1108 3 0.04048943465754462 1108 4 -1.2012265887051399E-4 1108 5 3.2299830755988523E-4 1108 6 0.060956232859268766 1108 7 0.028990095254606274 1108 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50-51 -0.11868894173921518 2214 52-53 -0.19448114166925734 2214 54-55 -0.1429480890767323 2214 56-57 -0.2013032020213643 2214 58-59 -0.19406456179552833 2214 60-61 -0.17561847317944768 2214 62-63 -0.16296277784191915 2214 64-65 -0.21572331256420796 2214 66-67 -0.17507403541758038 2214 68-69 -0.15061356733022535 2214 70-71 -0.07716881932567787 2214 72-73 -0.12449838960031911 2214 74-75 -0.16363402439277053 2214 76-77 -0.17854069898367442 2214 78-79 -0.1185092524760023 2214 80-81 -0.09758110994687286 2214 82-83 -0.09445664698911571 2214 84-85 -0.09046381246619717 2214 86-87 -0.15206046881022672 2214 88-89 -0.12284646105221952 2214 90-91 -0.10953269777736807 2214 92-93 -0.11877149454851121 2214 94-95 -0.1733413321572641 2214 96-97 -0.10004068202765382 2214 98-99 -0.12093556745782053 2214 100 -0.11819581946610214 2215 1 -0.21151985906838888 2215 2 -0.1655082045969536 2215 3 -0.205820124080212 2215 4 -0.12710014428986938 2215 5 -0.1664564907572128 2215 6 -0.1695423485308183 2215 7 -0.22751733832419063 2215 8 -0.1566132632437558 2215 9 -0.2253327489396213 2215 10-11 -0.1715482554896326 2215 12-13 -0.221509149065362 2215 14-15 -0.3226243266705282 2215 16-17 -0.3303703144642469 2215 18-19 -0.31284048360869576 2215 20-21 -0.26723162159242264 2215 22-23 -0.3004221174286812 2215 24-25 -0.30267828882875847 2215 26-27 -0.3623889019996085 2215 28-29 -0.34188172215013424 2215 30-31 -0.3721207223906404 2215 32-33 -0.33773677263156543 2215 34-35 -0.39592498946528565 2215 36-37 -0.4310168074459142 2215 38-39 -0.38787592230049484 2215 40-41 -0.37864246076097885 2215 42-43 -0.36104868473560714 2215 44-45 -0.3933950079253492 2215 46-47 -0.4794532937821785 2215 48-49 -0.4298612753115094 2215 50-51 -0.4124115071577066 2215 52-53 -0.4111513032204783 2215 54-55 -0.485375662411073 2215 56-57 -0.4211650020383786 2215 58-59 -0.42954715088918505 2215 60-61 -0.46235221854669817 2215 62-63 -0.4225861675934084 2215 64-65 -0.3957430279482139 2215 66-67 -0.4609272015054273 2215 68-69 -0.41460407001957833 2215 70-71 -0.4610051088983056 2215 72-73 -0.4050755486105615 2215 74-75 -0.4121082840815049 2215 76-77 -0.42069932836016477 2215 78-79 -0.45024423888973075 2215 80-81 -0.42641999693220356 2215 82-83 -0.4003550172721688 2215 84-85 -0.3848707991372393 2215 86-87 -0.37560049652244487 2215 88-89 -0.3514127127032509 2215 90-91 -0.33793971947355317 2215 92-93 -0.2837382203668639 2215 94-95 -0.30646760407252316 2215 96-97 -0.34728635720815504 2215 98-99 -0.32028923892085714 2215 100 -0.3840326685254887 2216 1 -0.20904544349596677 2216 2 -0.17139073655973647 2216 3 -0.20432081732288765 2216 4 -0.17021424730499035 2216 5 -0.20538760982532978 2216 6 -0.19262852483632287 2216 7 -0.20141161698898458 2216 8 -0.18366205372204547 2216 9 -0.21976105800538193 2216 10-11 -0.2826792899055661 2216 12-13 -0.311931407049606 2216 14-15 -0.3828779411510439 2216 16-17 -0.37255494579107307 2216 18-19 -0.3003309188833043 2216 20-21 -0.3235199853857935 2216 22-23 -0.3901217964939505 2216 24-25 -0.35825413796104044 2216 26-27 -0.4137332124939661 2216 28-29 -0.4059288391511231 2216 30-31 -0.4232066009440061 2216 32-33 -0.3947080204119118 2216 34-35 -0.43701169323426825 2216 36-37 -0.3654866845733764 2216 38-39 -0.3509179866425356 2216 40-41 -0.37799327597792143 2216 42-43 -0.3979544655891232 2216 44-45 -0.49368235319440856 2216 46-47 -0.4509489332416976 2216 48-49 -0.5043605256179831 2216 50-51 -0.4410497812145664 2216 52-53 -0.43529604673487654 2216 54-55 -0.43550979407560675 2216 56-57 -0.47637626272238265 2216 58-59 -0.4236007670988329 2216 60-61 -0.4206938997269063 2216 62-63 -0.4580869004239503 2216 64-65 -0.5016771579692687 2216 66-67 -0.498292431791036 2216 68-69 -0.46192685295925884 2216 70-71 -0.4302565125343847 2216 72-73 -0.4456921364400941 2216 74-75 -0.5238571060079309 2216 76-77 -0.5613824768015618 2216 78-79 -0.5339408424141538 2216 80-81 -0.5164140689237158 2216 82-83 -0.460542732276906 2216 84-85 -0.6199041435492845 2216 86-87 -0.6096673643618473 2216 88-89 -0.695531584650908 2216 90-91 -0.6547126644357419 2216 92-93 -0.6997101922652149 2216 94-95 -0.6139884580312298 2216 96-97 -0.6868628896393041 2216 98-99 -0.8162404361566757 2216 100 -0.8715432590824896 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21.0 3 8.0 4 14.0 5 30.0 6 72.0 7 213.0 8 436.0 9 793.0 10 1324.0 11 2043.0 12 3208.0 13 4804.0 14 5372.0 15 7216.0 16 9415.0 17 13681.0 18 16215.0 19 9070.0 20 6536.0 21 6319.0 22 6652.0 23 7191.0 24 8370.0 25 10134.0 26 13038.0 27 17019.0 28 21574.0 29 28121.0 30 35857.0 31 45693.0 32 58673.0 33 76575.0 34 107265.0 35 167892.0 36 317199.0 37 699597.0 38 1123892.0 39 457248.0 40 1276.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58557387736634 18.960443923734587 13.190913173793806 24.263069025105263 2 15.976994648720803 19.84482722373027 39.27494021210724 24.903237915441682 3 18.661860523362623 24.88215647868847 29.903621811355528 26.552361186593377 4 12.734617901584114 16.455768535767877 37.303269901278945 33.50634366136906 5 13.410924882881684 37.53223736181437 34.95580157012859 14.101036185175358 6 31.99819698843574 37.17070039339816 17.180432765327954 13.65066985283815 7 28.022623323128848 32.34391755033957 21.79485698723669 17.838602139294892 8 26.221729153052387 35.7412501329767 19.532742663485358 18.504278050485553 9 25.905090977174854 15.918391662634313 20.154641744699788 38.02187561549104 10-11 24.918849709159847 26.070842657568495 28.12825081780983 20.88205681546183 12-13 25.600339325531234 23.24009074617575 28.51077610837019 22.648793819922822 14-15 22.587197188608172 24.869781337204408 25.839297541095966 26.70372393309145 16-17 22.36976301731539 28.519790197749245 26.347391932129916 22.76305485280545 18-19 22.324211863685548 27.165232033653087 28.61112630849473 21.899429794166636 20-21 23.115032084560262 26.34505309332121 28.550729835601587 21.989184986516946 22-23 22.712915392496953 26.184624493662866 28.475713598360635 22.62674651547955 24-25 22.602172111484276 26.271597673512765 28.57284193476371 22.55338828023925 26-27 22.49877319512269 26.44979157614754 28.42248166099578 22.62895356773399 28-29 22.4615397240863 26.501538502220555 28.509927127258383 22.52699464643476 30-31 22.636671810454118 26.720709362432764 28.22284321863508 22.41977560847804 32-33 22.519844647021205 26.442893373243493 28.478451430613948 22.558810549121354 34-35 22.4409706096188 26.437133592862853 28.540517243475488 22.58137855404285 36-37 22.743442664805706 26.49413870159049 28.16056930337964 22.601849330224166 38-39 22.765843499320376 26.665290803560794 28.101983674442018 22.466882022676817 40-41 22.59647488961424 26.53970923872655 28.255076741924828 22.608739129734385 42-43 22.61628677445004 26.54439620480624 28.080859413943106 22.758457606800615 44-45 22.684583484293277 26.636309533941066 27.964919138154485 22.71418784361117 46-47 22.828502007260667 26.705609266225256 27.76837233165636 22.69751639485772 48-49 22.95292238186827 26.764772392931913 27.45667550947461 22.825629715725203 50-51 22.86942836231359 26.959845060388034 27.370719525746672 22.800007051551706 52-53 22.967770153456353 26.816519232499385 27.32755916616617 22.88815144787809 54-55 22.914609964085717 27.04773110244932 27.234673209209813 22.802985724255148 56-57 22.63142329492264 26.9534317956898 27.474501953766133 22.940642955621424 58-59 22.682866188295883 26.70649071018852 27.602600077323913 23.008043024191686 60-61 22.744628054963197 26.878737018458043 27.442633195301237 22.934001731277522 62-63 22.725798588230717 26.870120143851654 27.387390974500132 23.0166902934175 64-65 22.70903595561899 26.764362065569703 27.459882141823726 23.066719836987577 66-67 22.566002306040126 26.843300971670537 27.42937011244947 23.161326609839865 68-69 22.72691420810362 26.730776157251178 27.51239077478645 23.029918859858753 70-71 22.605591303854904 26.7413725999455 27.480372291592044 23.172663804607552 72-73 22.67438591265469 26.860954789372183 27.395749906156297 23.06890939181683 74-75 22.6777957680317 26.94757500867007 27.19843722882276 23.17619199447547 76-77 22.702242758177977 26.842339461699133 27.198123679353785 23.257294100769105 78-79 22.755205382522366 26.82720294122653 27.309975878830027 23.107615797421076 80-81 22.737120583965744 26.709894907563886 27.40050625278111 23.152478255689264 82-83 22.53538999663835 26.84082084974897 27.419686728546093 23.20410242506658 84-85 22.59786763507977 26.79071420060935 27.169613526335112 23.441804637975768 86-87 22.60598166252462 26.838561311668542 27.251991124116024 23.303465901690817 88-89 22.382863453804884 26.69236494082249 27.363251599670452 23.56152000570217 90-91 22.37310973308792 26.727971018655595 27.332777402648677 23.566141845607806 92-93 22.512643108345248 26.723299322028183 27.353794421850115 23.41026314777646 94-95 22.302783662972633 26.606840564486312 27.381984176158586 23.70839159638247 96-97 22.376507233474495 26.55937552335815 27.410897277884143 23.653219965283213 98-99 22.094191506062593 26.760424035786663 27.3936662104936 23.75171824765714 100 22.3348781905232 26.432407229542598 27.276526600155133 23.956187979779067 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 69.0 1 64.0 2 158.5 3 273.0 4 203.5 5 159.0 6 369.5 7 850.5 8 1537.5 9 1854.0 10 1513.0 11 1188.5 12 1195.0 13 1386.5 14 1650.0 15 1890.0 16 2125.0 17 2371.0 18 2704.0 19 3210.0 20 3704.0 21 4292.0 22 5327.5 23 7025.5 24 9564.0 25 12648.0 26 16658.0 27 21866.0 28 28039.0 29 34744.5 30 41510.5 31 48525.0 32 55298.5 33 63101.0 34 70858.0 35 76191.0 36 81156.5 37 88736.0 38 95062.5 39 98116.5 40 101683.0 41 106322.0 42 110135.0 43 111869.0 44 112475.5 45 114049.0 46 113194.5 47 108738.0 48 108887.5 49 110153.0 50 107140.5 51 104885.5 52 102802.0 53 103041.0 54 103192.5 55 98088.0 56 90011.0 57 83280.0 58 76177.0 59 67264.5 60 60804.0 61 55165.0 62 49092.0 63 41914.5 64 34842.5 65 29059.5 66 23255.5 67 17963.5 68 14392.0 69 11780.0 70 9194.5 71 6680.0 72 4967.0 73 3771.5 74 2685.0 75 1672.0 76 913.5 77 545.5 78 345.0 79 204.5 80 127.0 81 71.0 82 32.5 83 13.0 84 5.0 85 2.5 86 2.5 87 2.0 88 1.5 89 0.5 90 0.0 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.042035758661858644 2 0.24428155630177723 3 0.003039461942289128 4 0.002401174934408411 5 2.1276233596023898E-4 6 1.519730971144564E-4 7 0.0 8 1.823677165373477E-4 9 0.0 10-11 5.623004593234887E-4 12-13 0.0 14-15 3.9513005249758673E-4 16-17 1.6717040682590205E-4 18-19 4.8631391076626047E-4 20-21 0.0 22-23 1.519730971144564E-4 24-25 9.574305118210753E-4 26-27 1.6717040682590205E-4 28-29 1.6717040682590205E-4 30-31 9.118385826867385E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 9.118385826867385E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.519730971144564E-5 68-69 2.2795964567168462E-4 70-71 1.519730971144564E-5 72-73 3.343408136518041E-4 74-75 9.118385826867385E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.078923884578256E-5 84-85 0.0 86-87 5.319058399005974E-4 88-89 0.0024771614829656396 90-91 0.0023555830052740746 92-93 6.838789370150538E-4 94-95 6.230896981692713E-4 96-97 1.519730971144564E-4 98-99 1.0638116798011949E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3290056.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.10715726259528 #Duplication Level Percentage of deduplicated Percentage of total 1 69.65584504655791 34.90256382006846 2 15.706993775419747 15.740656144551252 3 5.878609090860095 8.836811706031474 4 2.8331346334690086 5.678412905013474 5 1.5948752918354656 3.995733353111361 6 0.9787939340221768 2.942674894783411 7 0.6695454126881608 2.348431210161045 8 0.48728983500108863 1.9533366715890923 9 0.35048760365547604 1.580574372745959 >10 1.726034636651372 15.060001596851407 >50 0.08369643605904584 2.862870484576894 >100 0.03250675057520219 2.9342180060267804 >500 0.00164066490552018 0.5283606372729481 >1k 4.2535756650251505E-4 0.29884513565344556 >5k 1.2153073328643287E-4 0.3365090615631055 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5980 0.18175982414888986 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5072 0.15416150971290457 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.5197309711445641E-5 0.0 20-21 0.0 0.0 0.0 7.59865485572282E-5 0.0 22-23 0.0 0.0 0.0 1.0638116798011949E-4 0.0 24-25 3.0394619422891283E-5 0.0 0.0 1.9756502624879334E-4 0.0 26-27 3.0394619422891283E-5 0.0 0.0 3.495381233632498E-4 0.0 28-29 3.0394619422891283E-5 0.0 0.0 0.001063811679801195 0.0 30-31 3.0394619422891283E-5 0.0 0.0 0.003890511286130084 0.0 32-33 3.0394619422891283E-5 0.0 0.0 0.010273381364937252 0.0 34-35 3.0394619422891283E-5 0.0 0.0 0.018966242519884158 0.0 36-37 3.0394619422891283E-5 0.0 0.0 0.036124005184106286 0.0 38-39 3.0394619422891283E-5 0.0 0.0 0.0652268532815247 0.0 40-41 3.0394619422891283E-5 0.0 0.0 0.09890409160208824 0.0 42-43 3.0394619422891283E-5 0.0 0.0 0.13343237926649273 0.0 44-45 3.0394619422891283E-5 0.0 0.0 0.17393320964749537 0.0 46-47 3.0394619422891283E-5 0.0 0.0 0.22645511201025148 0.0 48-49 3.0394619422891283E-5 0.0 0.0 0.2836881803835558 0.0 50-51 3.0394619422891283E-5 0.0 0.0 0.34627070177528896 0.0 52-53 3.0394619422891283E-5 0.0 0.0 0.4098866402274004 0.0 54-55 3.0394619422891283E-5 0.0 0.0 0.48096445774783164 0.0 56-57 3.0394619422891283E-5 0.0 0.0 0.5636530198878074 0.0 58-59 3.0394619422891283E-5 0.0 0.0 0.6626482953481643 0.0 60-61 3.0394619422891283E-5 0.0 0.0 0.7657924363597459 0.0 62-63 3.0394619422891283E-5 0.0 0.0 0.8743164250091792 0.0 64-65 3.0394619422891283E-5 0.0 0.0 0.987490790430315 0.0 66-67 3.0394619422891283E-5 0.0 0.0 1.1220781652348775 0.0 68-69 3.0394619422891283E-5 0.0 0.0 1.2756773744884586 0.0 70-71 3.0394619422891283E-5 0.0 0.0 1.4441699472592564 0.0 72-73 3.0394619422891283E-5 0.0 0.0 1.6205195291508716 0.0 74-75 3.0394619422891283E-5 0.0 0.0 1.7974770034309446 0.0 76-77 3.0394619422891283E-5 0.0 0.0 1.9928080251521556 0.0 78-79 3.0394619422891283E-5 0.0 0.0 2.216816370298864 0.0 80-81 3.0394619422891283E-5 0.0 0.0 2.46255686833294 0.0 82-83 3.0394619422891283E-5 0.0 0.0 2.719345810527237 0.0 84-85 3.0394619422891283E-5 0.0 0.0 2.975481268403942 0.0 86-87 3.0394619422891283E-5 0.0 0.0 3.252953749115517 0.0 88 3.0394619422891283E-5 0.0 0.0 3.480335897018166 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4625 0.0 49.935017 1 GTATCAA 7355 0.0 41.333088 1 TCAACGC 8915 0.0 33.634945 4 ATCAACG 8990 0.0 33.40713 3 CAACGCA 9280 0.0 32.310543 5 TATCAAC 9365 0.0 32.243298 2 AACGCAG 9645 0.0 31.13653 6 GTACATG 10750 0.0 27.314949 1 ACGCAGA 11520 0.0 26.068737 7 TACATGG 11100 0.0 25.845415 2 CGCAGAG 11760 0.0 25.376865 8 ACATGGG 10635 0.0 25.278807 3 GTGGTAT 2020 0.0 25.199575 1 CATGGGG 8765 0.0 22.30657 4 GCAGAGT 13040 0.0 22.129032 9 GAGTACA 6715 0.0 20.49546 1 GAGTACT 8350 0.0 19.418135 12-13 CAGAGTA 12975 0.0 19.142967 10-11 AGAGTAC 12265 0.0 18.699234 10-11 ATGGGGG 4045 0.0 18.241173 5 >>END_MODULE