FastQCFastQC Report
Wed 25 May 2016
SRR1033023_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033023_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2521639
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC30300.033.8214454
GTATCAA32400.033.520791
ATCAACG30500.033.445543
GGTATCA20800.032.323621
CAACGCA32500.031.3885975
AACGCAG34550.029.6551766
GTACATG67700.026.7374111
TATCAAC39750.026.253922
TACATGG66950.026.0496352
ACGCAGA40800.024.6516347
ACATGGG72500.023.6664523
CGCAGAG41850.023.4893328
TATACCG1554.2337146E-521.2305815
GAGTACA49200.020.832371
TACCGAT1605.4166987E-520.5622277
CATGGGG49700.019.8628334
AGTACAT51800.018.9668332
GCAGAGT51900.018.4913629
GTATAGA10600.017.7419531
GTCTCGC6800.016.5939451
Produced by FastQC (version 0.11.5)