FastQCFastQC Report
Wed 25 May 2016
SRR1033023_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033023_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2521639
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47140.18694190564152918No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37840.1500611308755932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37000.051.100111
GTATCAA69450.043.6533851
TCAACGC81850.036.460254
ATCAACG82050.036.3155363
CAACGCA83600.035.640815
AACGCAG86800.034.4892926
TATCAAC88700.033.896252
ACGCAGA100200.029.7831487
CGCAGAG102350.029.0197628
GCAGAGT112700.026.1044799
GTACATG64250.025.6846771
TACATGG64200.024.5169282
ACATGGG68050.022.9984173
GAGTACT79700.022.0535212-13
AGAGTAC103350.021.12223410-11
CAGAGTA112750.020.77845210-11
AGTACTT84750.020.0739812-13
GTACTTT87300.019.8375414-15
GTATTAG12450.019.693061
ACACCGT3055.2150426E-918.4903876
Produced by FastQC (version 0.11.5)