##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033023_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2521639
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71132743426002	34.0	31.0	34.0	31.0	34.0
2	32.70687834380734	34.0	31.0	34.0	31.0	34.0
3	32.80809624216631	34.0	31.0	34.0	31.0	34.0
4	36.30199247394254	37.0	37.0	37.0	35.0	37.0
5	36.28723461209158	37.0	37.0	37.0	35.0	37.0
6	36.35424261759911	37.0	37.0	37.0	35.0	37.0
7	36.306326163261275	37.0	37.0	37.0	35.0	37.0
8	36.31671861039586	37.0	37.0	37.0	35.0	37.0
9	38.121774766332535	39.0	39.0	39.0	37.0	39.0
10-11	38.0825629679744	39.0	39.0	39.0	36.0	39.0
12-13	38.0580420115647	39.0	39.0	39.0	35.0	39.0
14-15	39.55243871148883	41.0	40.0	41.0	37.0	41.0
16-17	39.549681377865745	41.0	40.0	41.0	37.0	41.0
18-19	39.56924841343269	41.0	40.0	41.0	37.0	41.0
20-21	39.562779208284766	41.0	40.0	41.0	37.0	41.0
22-23	39.49083235149837	41.0	39.0	41.0	36.5	41.0
24-25	39.43441626656313	41.0	39.0	41.0	36.0	41.0
26-27	39.32103306619227	41.0	39.0	41.0	36.0	41.0
28-29	39.26874961086817	41.0	39.0	41.0	36.0	41.0
30-31	39.26058587291837	41.0	39.0	41.0	36.0	41.0
32-33	39.21196174392925	41.0	39.0	41.0	35.0	41.0
34-35	39.12458642969909	41.0	39.0	41.0	35.0	41.0
36-37	39.03180490942597	41.0	39.0	41.0	35.0	41.0
38-39	38.93447476026505	41.0	39.0	41.0	35.0	41.0
40-41	38.827408681417126	41.0	39.0	41.0	35.0	41.0
42-43	38.70702844459497	41.0	39.0	41.0	35.0	41.0
44-45	38.53340803342588	41.0	38.0	41.0	35.0	41.0
46-47	38.32526483767106	41.0	38.0	41.0	34.5	41.0
48-49	38.11659638830142	40.0	38.0	41.0	34.0	41.0
50-51	37.97730900418339	40.0	38.0	41.0	34.0	41.0
52-53	37.84108153466852	40.0	38.0	41.0	33.5	41.0
54-55	37.69326358769038	40.0	37.5	41.0	33.0	41.0
56-57	37.54301230271264	40.0	37.0	41.0	33.0	41.0
58-59	37.3648811348492	40.0	37.0	41.0	33.0	41.0
60-61	37.174020746030656	40.0	36.0	41.0	33.0	41.0
62-63	36.946287117228124	39.0	36.0	41.0	32.5	41.0
64-65	36.68291238357275	39.0	35.0	41.0	32.0	41.0
66-67	36.40833204118432	39.0	35.0	41.0	32.0	41.0
68-69	36.10916312763246	38.0	35.0	40.5	31.0	41.0
70-71	35.785776036934706	37.0	35.0	40.0	31.0	41.0
72-73	35.444410559957234	37.0	35.0	39.5	31.0	41.0
74-75	35.09977459104971	36.0	35.0	39.0	31.0	41.0
76-77	34.74623766526454	36.0	35.0	39.0	30.5	40.5
78-79	34.385864709421135	35.0	34.5	37.5	30.0	39.5
80-81	34.05569433213874	35.0	34.0	37.0	30.0	39.0
82-83	33.75278340793429	35.0	34.0	37.0	30.0	39.0
84-85	33.52505632249501	35.0	34.0	36.0	30.0	38.0
86-87	33.30910193727175	35.0	34.0	36.0	30.0	37.0
88-89	33.09734977925072	35.0	34.0	36.0	30.0	37.0
90-91	32.91042730541525	35.0	34.0	35.0	29.0	36.0
92-93	32.732783122405706	35.0	34.0	35.0	29.0	36.0
94-95	32.572633909929216	35.0	34.0	35.0	29.0	36.0
96-97	32.4266488978002	35.0	34.0	35.0	29.0	36.0
98-99	32.301807673501244	35.0	34.0	35.0	29.0	35.5
100	32.20100775725629	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15917589031359114
1101	2	0.14385347088587963
1101	3	0.16926466429274
1101	4	0.13406669623929446
1101	5	0.14041901143345115
1101	6	0.11224130304466229
1101	7	0.249054007197266
1101	8	0.20351674032222888
1101	9	0.23389546207224754
1101	10-11	0.2285783869136111
1101	12-13	0.21380278719264822
1101	14-15	0.3061673428478642
1101	16-17	0.3321958653485808
1101	18-19	0.34668307928385644
1101	20-21	0.30447875738930463
1101	22-23	0.30055096577598306
1101	24-25	0.3351093582164921
1101	26-27	0.36351072943666196
1101	28-29	0.3675331048983992
1101	30-31	0.3723543675579606
1101	32-33	0.339470560106065
1101	34-35	0.366085170749912
1101	36-37	0.31803760910189993
1101	38-39	0.28927929058097845
1101	40-41	0.3505675471761549
1101	42-43	0.3878934667749121
1101	44-45	0.3596388930587864
1101	46-47	0.36902690337004174
1101	48-49	0.40154314442652606
1101	50-51	0.35910705803052423
1101	52-53	0.3466211965571233
1101	54-55	0.3809416963277741
1101	56-57	0.42380205294530526
1101	58-59	0.3710105667812016
1101	60-61	0.3249959152446351
1101	62-63	0.3634058690002533
1101	64-65	0.3664103597643589
1101	66-67	0.36902700816942513
1101	68-69	0.37574854968170257
1101	70-71	0.3155727360660876
1101	72-73	0.32012397417224747
1101	74-75	0.2317353204174637
1101	76-77	0.27504409487532655
1101	78-79	0.33427650706705236
1101	80-81	0.2997470560878952
1101	82-83	0.30816850621747705
1101	84-85	0.3581375628423942
1101	86-87	0.37757983439797016
1101	88-89	0.40057038410790824
1101	90-91	0.42219160717937854
1101	92-93	0.36833034001151077
1101	94-95	0.36958374365234903
1101	96-97	0.42106972244325647
1101	98-99	0.4090829632369619
1101	100	0.37615965642439164
1102	1	0.1442904647022516
1102	2	0.10369867041065817
1102	3	0.1561066410353149
1102	4	0.0921057531312428
1102	5	0.13016135507658788
1102	6	0.17278855055354825
1102	7	0.18084290579469808
1102	8	0.1629342233373734
1102	9	0.19720908948695381
1102	10-11	0.21965361755238177
1102	12-13	0.2203864684026513
1102	14-15	0.28456984396127893
1102	16-17	0.30584983069702787
1102	18-19	0.2839916371612148
1102	20-21	0.251426359203748
1102	22-23	0.23352109707775526
1102	24-25	0.29749408167703706
1102	26-27	0.3130119496111021
1102	28-29	0.3551211864818953
1102	30-31	0.3272367446610076
1102	32-33	0.3356684927753406
1102	34-35	0.28723791986521974
1102	36-37	0.32072754496326894
1102	38-39	0.36429302943160735
1102	40-41	0.4471039002520243
1102	42-43	0.4414515077853096
1102	44-45	0.36975376304479823
1102	46-47	0.3658584425274398
1102	48-49	0.4032406014839438
1102	50-51	0.4210965207133839
1102	52-53	0.396264971765369
1102	54-55	0.41025351485807704
1102	56-57	0.48288870683646223
1102	58-59	0.4563260445496269
1102	60-61	0.46983437429309305
1102	62-63	0.45148646717275653
1102	64-65	0.5189990385401728
1102	66-67	0.501942184068696
1102	68-69	0.4962668440730198
1102	70-71	0.44583160565727553
1102	72-73	0.4832816290039972
1102	74-75	0.511677018338915
1102	76-77	0.4384605044865424
1102	78-79	0.44948565889801984
1102	80-81	0.4752885261680504
1102	82-83	0.4604496352314058
1102	84-85	0.5330168520241685
1102	86-87	0.5098468381427139
1102	88-89	0.541825089460751
1102	90-91	0.5077176445075793
1102	92-93	0.6268187819838644
1102	94-95	0.6160014465233772
1102	96-97	0.6818571834950902
1102	98-99	0.6608867068188573
1102	100	0.6946454063188767
1103	1	0.10333094860599346
1103	2	0.12962262485013554
1103	3	0.13984684380277201
1103	4	0.10011722106619203
1103	5	0.08941750359252865
1103	6	0.09651804156781196
1103	7	0.10678302070244428
1103	8	0.11141034540338524
1103	9	0.2180726894658278
1103	10-11	0.17189799603859512
1103	12-13	0.17138237471860407
1103	14-15	0.17184421050912846
1103	16-17	0.20497059277442986
1103	18-19	0.20994502057748576
1103	20-21	0.22979339112547592
1103	22-23	0.22248071209219233
1103	24-25	0.19612974215339563
1103	26-27	0.18106378010249813
1103	28-29	0.23065583959501623
1103	30-31	0.26546822254199753
1103	32-33	0.25518890890205626
1103	34-35	0.2791090482232974
1103	36-37	0.2905376623365825
1103	38-39	0.28049200513560635
1103	40-41	0.2191211415902501
1103	42-43	0.2323446607994768
1103	44-45	0.22990031474839157
1103	46-47	0.12352540164901171
1103	48-49	0.08411978518229546
1103	50-51	0.17477425961492798
1103	52-53	0.19355970773313658
1103	54-55	0.16223337761650924
1103	56-57	0.17539192557944716
1103	58-59	0.059559929247683385
1103	60-61	0.12692529939733532
1103	62-63	0.14325979471011863
1103	64-65	0.08784957573402608
1103	66-67	0.06100807942559072
1103	68-69	0.08021575267451908
1103	70-71	0.07415923873528385
1103	72-73	0.13881980997938825
1103	74-75	0.13593984588518992
1103	76-77	0.22487111988489517
1103	78-79	0.1610150972818971
1103	80-81	0.15709831293776944
1103	82-83	0.2096252418466662
1103	84-85	0.19684649521959585
1103	86-87	0.18063636862401466
1103	88-89	0.19011788428270648
1103	90-91	0.20975299263586322
1103	92-93	0.14565607942447656
1103	94-95	0.19248281388139077
1103	96-97	0.28176235679207906
1103	98-99	0.25572906091681347
1103	100	0.20599688237266633
1104	1	0.07280843412137017
1104	2	0.07768894662430625
1104	3	0.08725775464725416
1104	4	0.015563163605229136
1104	5	0.08627789281786846
1104	6	0.03351180632829909
1104	7	0.09886570379535442
1104	8	0.08594694356540344
1104	9	0.10464151703816782
1104	10-11	0.1132495562621827
1104	12-13	0.10715147529374747
1104	14-15	0.17495566492448944
1104	16-17	0.17917367355923375
1104	18-19	0.18566343970589827
1104	20-21	0.16971577067948118
1104	22-23	0.15744816145590335
1104	24-25	0.2046073195598339
1104	26-27	0.19920122711788224
1104	28-29	0.19584397669646592
1104	30-31	0.1542492288981876
1104	32-33	0.2134535661131025
1104	34-35	0.1710744327873286
1104	36-37	0.1611606443659923
1104	38-39	0.12207831949566383
1104	40-41	0.06766580731497385
1104	42-43	0.06937592810268
1104	44-45	0.046070154535961194
1104	46-47	0.05604953090279707
1104	48-49	-0.0017062564262815272
1104	50-51	0.06666876240975483
1104	52-53	0.08152268742207269
1104	54-55	0.1350641402258148
1104	56-57	0.14398164147903714
1104	58-59	0.10017113822755164
1104	60-61	0.10178324133632799
1104	62-63	0.11721084270002535
1104	64-65	0.1709249727308375
1104	66-67	0.13780288043435007
1104	68-69	0.20587086908162178
1104	70-71	0.2169671149761001
1104	72-73	0.13783849999004616
1104	74-75	0.15172193686989743
1104	76-77	0.1630689322496579
1104	78-79	0.13038749713832942
1104	80-81	0.0631134851706534
1104	82-83	0.16142257574966834
1104	84-85	0.15962064666075548
1104	86-87	0.16256069281248386
1104	88-89	0.16588676494765053
1104	90-91	0.18499935096225784
1104	92-93	0.13488236466213976
1104	94-95	0.21139332378245257
1104	96-97	0.23216561711784323
1104	98-99	0.21017387798688247
1104	100	0.22802245949949196
1105	1	0.0758585687454314
1105	2	0.10330989487373898
1105	3	0.08143738476012885
1105	4	0.06770067719506301
1105	5	0.019269042398555314
1105	6	0.06910406470302632
1105	7	0.08979016292160225
1105	8	0.029094126198408787
1105	9	0.025680606326744737
1105	10-11	0.04769943114816044
1105	12-13	0.10926490947402812
1105	14-15	0.1445098953305859
1105	16-17	0.14044123954555943
1105	18-19	0.12877163119388513
1105	20-21	0.0983094418650623
1105	22-23	0.15652340561654654
1105	24-25	0.11642292986444858
1105	26-27	0.19856454926995326
1105	28-29	0.20132464812017759
1105	30-31	0.18733178893884173
1105	32-33	0.12751723465869702
1105	34-35	0.08793104656044193
1105	36-37	0.07108478471585755
1105	38-39	0.035835042139666484
1105	40-41	0.054632608161895746
1105	42-43	0.04092977658575592
1105	44-45	0.05839485605399375
1105	46-47	0.06826943183105527
1105	48-49	0.03449635601847234
1105	50-51	-0.004870668859702221
1105	52-53	0.03843644509571931
1105	54-55	0.08179497046711504
1105	56-57	0.06539272531114193
1105	58-59	0.044080721376076326
1105	60-61	0.058037953558851996
1105	62-63	0.060786471446505175
1105	64-65	0.05905386185980177
1105	66-67	0.01479951236022714
1105	68-69	0.016513892025159294
1105	70-71	-0.004380175538742037
1105	72-73	0.011078363623745702
1105	74-75	-0.017933670513237132
1105	76-77	0.032301647379973986
1105	78-79	0.0317874504250355
1105	80-81	0.035741092727256785
1105	82-83	0.05825406020532142
1105	84-85	0.03873447874092051
1105	86-87	0.01628371949916385
1105	88-89	-0.03478251330152915
1105	90-91	-0.04310721621169478
1105	92-93	0.018564969894484307
1105	94-95	-0.06372267962898803
1105	96-97	-0.09700470457242005
1105	98-99	-0.08711591409331731
1105	100	-0.051209677420672506
1106	1	-0.020163630496085716
1106	2	-0.002231216789816415
1106	3	0.017067767918710786
1106	4	0.03565606381988573
1106	5	-0.013707113699098272
1106	6	0.04105902095481184
1106	7	0.03714688652880227
1106	8	0.04061852690690415
1106	9	0.07771144011120867
1106	10-11	0.09362420035252228
1106	12-13	0.0814694202661741
1106	14-15	0.11495953947802917
1106	16-17	0.09943588137187476
1106	18-19	0.1289874800487567
1106	20-21	0.1430688627513419
1106	22-23	0.13986459134429197
1106	24-25	0.1326647282702993
1106	26-27	0.10965472802194398
1106	28-29	0.10562188016218954
1106	30-31	0.13822253159137432
1106	32-33	0.16570963995823718
1106	34-35	0.12062618702483974
1106	36-37	0.1720267629540544
1106	38-39	0.15372755809393368
1106	40-41	0.22201206176477228
1106	42-43	0.23304033535537627
1106	44-45	0.30523377054841205
1106	46-47	0.36346581234086983
1106	48-49	0.2622339715340445
1106	50-51	0.3125763580191787
1106	52-53	0.2992041605243614
1106	54-55	0.26097369537956183
1106	56-57	0.28006405219358754
1106	58-59	0.26860132055424657
1106	60-61	0.313472516987801
1106	62-63	0.3466924096229178
1106	64-65	0.2893612415209361
1106	66-67	0.29904702778021175
1106	68-69	0.2816777708342286
1106	70-71	0.2910266079291688
1106	72-73	0.34633082362363865
1106	74-75	0.38435807324230353
1106	76-77	0.33318795972187587
1106	78-79	0.33338478346635014
1106	80-81	0.3415564936056015
1106	82-83	0.3273747509923126
1106	84-85	0.3383030312480457
1106	86-87	0.2845136013997447
1106	88-89	0.23811133763337722
1106	90-91	0.29060394460917394
1106	92-93	0.38481823412085703
1106	94-95	0.3342144441073813
1106	96-97	0.3462822782918451
1106	98-99	0.3398352182558071
1106	100	0.3623232436141208
1107	1	0.07132384270862957
1107	2	0.13358402972679784
1107	3	0.09190197209877482
1107	4	0.05477282427004582
1107	5	0.07380963335342017
1107	6	0.0954743813513872
1107	7	0.09293383052666115
1107	8	0.057702195181285276
1107	9	0.08781326219052232
1107	10-11	0.14450677002025714
1107	12-13	0.1318730242318793
1107	14-15	0.19871489853483837
1107	16-17	0.10976253263125102
1107	18-19	0.14148999210663504
1107	20-21	0.07094391299250447
1107	22-23	0.1334065092167549
1107	24-25	0.1983089873806989
1107	26-27	0.15589920149461278
1107	28-29	0.19047333380634512
1107	30-31	0.1839352861465713
1107	32-33	0.18895203679639394
1107	34-35	0.19668543809665096
1107	36-37	0.19306817841492574
1107	38-39	0.15042838874774134
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2216	28-29	-0.3678005368978461
2216	30-31	-0.33280517347814254
2216	32-33	-0.27427782167926296
2216	34-35	-0.33534251757486544
2216	36-37	-0.32931571297765316
2216	38-39	-0.32337840547532437
2216	40-41	-0.24288518399865922
2216	42-43	-0.2829502291793986
2216	44-45	-0.3762873304411727
2216	46-47	-0.33760915140668857
2216	48-49	-0.3345129352292133
2216	50-51	-0.336174500412902
2216	52-53	-0.3564242388836689
2216	54-55	-0.2802460442536656
2216	56-57	-0.3921270020796612
2216	58-59	-0.3504838874320626
2216	60-61	-0.3846849640317842
2216	62-63	-0.46048128618738815
2216	64-65	-0.47241792127304194
2216	66-67	-0.43996546516081736
2216	68-69	-0.39926496894882035
2216	70-71	-0.35505463686345706
2216	72-73	-0.34184723603849676
2216	74-75	-0.47004870865934123
2216	76-77	-0.45424578084553957
2216	78-79	-0.47838834572792877
2216	80-81	-0.4630136022472797
2216	82-83	-0.40233337192770335
2216	84-85	-0.4611721472685417
2216	86-87	-0.545103729403543
2216	88-89	-0.6290609141805987
2216	90-91	-0.6050090182746999
2216	92-93	-0.6209219902612588
2216	94-95	-0.5947836461566283
2216	96-97	-0.7770898361729941
2216	98-99	-0.8330722412394138
2216	100	-0.8294694676618626
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28.0
3	3.0
4	8.0
5	15.0
6	60.0
7	136.0
8	320.0
9	662.0
10	1060.0
11	1871.0
12	2924.0
13	4468.0
14	5316.0
15	7040.0
16	8802.0
17	12051.0
18	14093.0
19	7497.0
20	5083.0
21	4729.0
22	4826.0
23	5237.0
24	6047.0
25	7317.0
26	9376.0
27	11931.0
28	15660.0
29	20002.0
30	25846.0
31	33005.0
32	42493.0
33	54877.0
34	76817.0
35	116168.0
36	211070.0
37	477182.0
38	879592.0
39	446371.0
40	1656.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.88820165320077	19.84479670232666	13.513954212646768	23.753047431825802
2	15.575209488378453	20.307512003988208	40.382259143027746	23.735019364605588
3	18.115499181260482	25.559861974278615	30.34333124864419	25.981307595816716
4	12.074034152220442	16.903401936791543	39.320183709266324	31.70238020172169
5	12.301269732245045	37.91125119664471	36.1816584008623	13.605820670247942
6	30.55433390743084	38.702922979855565	17.578963523327488	13.16377958938611
7	26.743915366156696	32.857915030660614	22.875122093210013	17.52304750997268
8	24.994745470595653	36.267634398965114	20.41818915829974	18.319430972139493
9	25.027135129175903	16.156476006280045	21.530798024618115	37.28559083992594
10-11	23.705220361782438	26.660555530439485	29.161719461966207	20.47250464581187
12-13	24.803649531118452	24.064566736158508	29.352754299881944	21.779029432841103
14-15	21.751620628002396	25.95312834295112	26.915957750352398	25.37929327869408
16-17	20.854009402629643	29.421724397067777	27.3875481582386	22.33671804206398
18-19	20.610998375255342	27.906387423675273	30.270773591811956	21.211840609257436
20-21	21.850752992548298	27.203165719432025	29.974518552123946	20.971562735895727
22-23	21.403307218012433	27.031478770131773	30.066770144461767	21.498443867394027
24-25	21.289836203659014	27.194820604479602	29.99418624710353	21.52115694475785
26-27	21.065281057726434	27.130155633150714	30.11770141198072	21.686861897142133
28-29	21.046373105692577	27.205851443944677	30.176901168052144	21.570874282310598
30-31	21.33044922802132	27.643035060161314	29.710303267282324	21.316212444535036
32-33	21.02570986568656	27.278210719298045	30.269995031009593	21.4260843840058
34-35	21.184971361880113	27.10600129518936	30.172796343965175	21.536230998965355
36-37	21.541604488192007	27.578015727072746	29.520998049284614	21.359381735450633
38-39	21.378476459160094	27.71384008575375	29.59928046798134	21.308402987104817
40-41	21.188969469243695	27.43913429269671	29.76200980513654	21.60988643292305
42-43	21.26055712177675	27.33912348278243	29.641197649623912	21.759121745816906
44-45	21.425330905811656	27.614916330212214	29.41717668548115	21.542576078494978
46-47	21.527565999732715	27.861065759214544	29.05429762150728	21.557070619545463
48-49	21.54291316084499	27.773939885923397	28.79765898290755	21.885487970324064
50-51	21.643918895607182	27.765512827173122	28.65513263397338	21.93543564324632
52-53	21.593356543105497	27.782803168891345	28.69843780176306	21.9254024862401
54-55	21.746748840734142	28.18214661178702	28.48920880427373	21.581895743205116
56-57	21.49839846226998	27.8678272345883	28.589599859456488	22.04417444368524
58-59	21.345244105123694	27.507922426643937	29.01049674437935	22.136336723853017
60-61	21.677468503620066	27.892335104271464	28.564854049290954	21.86534234281751
62-63	21.496058714193428	27.85208350600542	28.627967762237176	22.023890017563975
64-65	21.504921997161368	27.62738837716263	28.76428783025643	22.10340179541957
66-67	21.351688326520964	27.713304719668436	28.64747888179077	22.287528072019825
68-69	21.841485624887845	27.855744245047603	28.454998894565325	21.847771235499224
70-71	21.762179630426804	27.66601953452091	28.516617270873677	22.05518356417861
72-73	21.542604766518178	27.820069629537393	28.600695343608955	22.036630260335475
74-75	21.68230136399598	27.840448756461583	28.40026411435438	22.076985765188063
76-77	21.655696949484046	27.846154822319928	28.310198248044227	22.187949980151796
78-79	21.799512142697665	27.97337366688888	28.333932811159723	21.893181379253733
80-81	21.684229978993823	27.767158582176116	28.48367668805884	22.064934750771226
82-83	21.55825058776338	27.825651248961243	28.596995878270278	22.01910228500511
84-85	21.509879090543887	27.77542701393816	28.448540810163546	22.266153085354407
86-87	21.63218591824437	27.831126323526757	28.385947233618648	22.150740524610228
88-89	21.389481932959484	27.807068107104456	28.415671116033923	22.387778843902144
90-91	21.372689881192333	27.660412758345753	28.57302903140927	22.393868329052648
92-93	21.49777712652186	27.852215051136696	28.311859403277296	22.338148419064147
94-95	21.272001441927614	27.58705747029346	28.53406603560522	22.60687505217371
96-97	21.20149617449976	27.624700045327806	28.550033688513505	22.62377009165893
98-99	21.213557870191362	27.70668633644441	28.402028842397886	22.67772695096634
100	21.381252431454303	27.565365224760562	28.307422275750017	22.745960068035114
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	23.5
2	92.0
3	169.0
4	114.5
5	89.0
6	247.0
7	563.5
8	979.0
9	1170.0
10	970.5
11	754.0
12	820.5
13	1011.5
14	1164.5
15	1369.0
16	1580.5
17	1832.5
18	2191.0
19	2579.0
20	3132.5
21	3967.0
22	5228.5
23	7198.0
24	9696.5
25	12707.5
26	16598.0
27	21741.0
28	28628.5
29	36236.5
30	43301.5
31	50464.5
32	58213.0
33	65698.5
34	71967.5
35	77332.0
36	81927.5
37	86226.5
38	89164.5
39	89927.5
40	90651.5
41	91282.5
42	90524.0
43	89022.5
44	87413.0
45	85944.0
46	84803.5
47	82557.0
48	80243.5
49	78189.0
50	75113.5
51	72144.5
52	67960.0
53	64015.5
54	61467.0
55	58680.0
56	54438.5
57	49412.5
58	44994.0
59	41035.5
60	36902.0
61	31537.0
62	26569.5
63	22237.5
64	18254.5
65	14937.0
66	11884.5
67	8788.5
68	6354.5
69	4857.5
70	3550.5
71	2538.5
72	1854.0
73	1392.0
74	1043.0
75	703.0
76	454.0
77	300.5
78	205.0
79	134.0
80	83.5
81	38.5
82	16.5
83	8.0
84	3.0
85	1.0
86	1.0
87	2.0
88	1.5
89	0.5
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.042591346342597015
2	0.24686325044941007
3	0.003172539764811696
4	0.001824210364766725
5	1.98283735300731E-4
6	0.0
7	0.0
8	1.98283735300731E-4
9	0.0
10-11	5.155377117819006E-4
12-13	0.0
14-15	3.370823500112427E-4
16-17	1.586269882405848E-4
18-19	4.7588096472175434E-4
20-21	1.98283735300731E-5
22-23	1.1897024118043858E-4
24-25	0.0012690159059246784
26-27	1.586269882405848E-4
28-29	1.98283735300731E-4
30-31	7.93134941202924E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	5.948512059021929E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	2.577688558909503E-4
70-71	1.98283735300731E-5
72-73	3.172539764811696E-4
74-75	1.586269882405848E-4
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.387986147105117E-4
84-85	0.0
86-87	5.750228323721198E-4
88-89	0.0017647252441765058
90-91	0.0016854117500562135
92-93	6.146795794322661E-4
94-95	5.750228323721198E-4
96-97	3.172539764811696E-4
98-99	1.586269882405848E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2521639.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.42417435535455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.25723109511358	50.544905573786295
2	13.998469278634737	18.316765895869462
3	4.329776706132974	8.498161984253892
4	1.7393758610675567	4.551889184159151
5	0.8304612449007551	2.716612064087589
6	0.4751324596969854	1.8651089331062427
7	0.31181517101237344	1.428017508047075
8	0.20679355401473637	1.0823438026738832
9	0.14078897259794987	0.8289902061503552
>10	0.6739840806560612	7.529541367833895
>50	0.02745608174852026	1.2282078086051318
>100	0.00835128879403696	0.899817616966375
>500	1.2140187658056954E-4	0.044915502191260635
>1k	2.4280375314869688E-4	0.4647225522693438
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4714	0.18694190564152918	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3784	0.1500611308755932	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.93134941202924E-5	0.0
3	0.0	0.0	0.0	7.93134941202924E-5	0.0
4	0.0	0.0	0.0	1.189702411804386E-4	0.0
5	0.0	0.0	0.0	1.189702411804386E-4	0.0
6	0.0	0.0	0.0	1.189702411804386E-4	0.0
7	0.0	0.0	0.0	1.189702411804386E-4	0.0
8	0.0	0.0	0.0	1.189702411804386E-4	0.0
9	0.0	0.0	0.0	1.586269882405848E-4	0.0
10-11	3.96567470601462E-5	0.0	0.0	1.98283735300731E-4	0.0
12-13	3.96567470601462E-5	0.0	0.0	3.172539764811696E-4	0.0
14-15	5.9485120590219306E-5	0.0	0.0	3.172539764811696E-4	0.0
16-17	7.93134941202924E-5	0.0	0.0	3.172539764811696E-4	0.0
18-19	7.93134941202924E-5	0.0	0.0	3.370823500112427E-4	0.0
20-21	7.93134941202924E-5	0.0	0.0	4.560525911916813E-4	0.0
22-23	7.93134941202924E-5	0.0	0.0	5.353660853119736E-4	0.0
24-25	7.93134941202924E-5	0.0	0.0	5.750228323721199E-4	0.0
26-27	7.93134941202924E-5	0.0	0.0	5.94851205902193E-4	0.0
28-29	7.93134941202924E-5	0.0	0.0	9.121051823833626E-4	0.0
30-31	1.1897024118043861E-4	0.0	0.0	0.002736315547150088	0.0
32-33	1.586269882405848E-4	0.0	0.0	0.008327916882630702	0.0
34-35	1.586269882405848E-4	0.0	0.0	0.014732481532844312	0.0
36-37	1.586269882405848E-4	0.0	0.0	0.026907102880309196	0.0
38-39	1.98283735300731E-4	0.0	0.0	0.05006664316343458	0.0
40-41	1.98283735300731E-4	0.0	0.0	0.08028508442326598	0.0
42-43	1.98283735300731E-4	0.0	0.0	0.11266481839787534	0.0
44-45	1.98283735300731E-4	0.0	0.0	0.15077495232267585	0.0
46-47	1.98283735300731E-4	0.0	0.0	0.19812510831249042	0.0
48-49	1.98283735300731E-4	0.0	0.0	0.25072978328777434	0.0
50-51	1.98283735300731E-4	0.0	0.0	0.31211842773688064	0.0
52-53	1.98283735300731E-4	0.0	0.0	0.37364587080069744	0.0
54-55	1.98283735300731E-4	0.0	0.0	0.4407054300794047	0.0
56-57	1.98283735300731E-4	0.0	0.0	0.518630938052592	0.0
58-59	1.98283735300731E-4	0.0	0.0	0.614382153829315	0.0
60-61	1.98283735300731E-4	0.0	0.0	0.7155861723268081	0.0
62-63	1.98283735300731E-4	0.0	0.0	0.8216877990862292	0.0
64-65	1.98283735300731E-4	0.0	0.0	0.9320723545281462	0.0
66-67	1.98283735300731E-4	0.0	0.0	1.0587954897588434	0.0
68-69	1.98283735300731E-4	0.0	0.0	1.207587604728512	0.0
70-71	1.98283735300731E-4	0.0	0.0	1.3692879908662579	0.0
72-73	1.98283735300731E-4	0.0	0.0	1.542568147145567	0.0
74-75	1.98283735300731E-4	0.0	0.0	1.7179897677661233	0.0
76-77	1.98283735300731E-4	0.0	0.0	1.907152451243021	0.0
78-79	1.98283735300731E-4	0.0	0.0	2.124729193988513	0.0
80-81	1.98283735300731E-4	0.0	0.0	2.364474058340627	0.0
82-83	1.98283735300731E-4	0.0	0.0	2.615203841628401	0.0
84-85	1.98283735300731E-4	0.0	0.0	2.870434665707502	0.0
86-87	1.98283735300731E-4	0.0	0.0	3.142182524937154	0.0
88	1.98283735300731E-4	0.0	0.0	3.364042196365142	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3700	0.0	51.10011	1
GTATCAA	6945	0.0	43.653385	1
TCAACGC	8185	0.0	36.46025	4
ATCAACG	8205	0.0	36.315536	3
CAACGCA	8360	0.0	35.64081	5
AACGCAG	8680	0.0	34.489292	6
TATCAAC	8870	0.0	33.89625	2
ACGCAGA	10020	0.0	29.783148	7
CGCAGAG	10235	0.0	29.019762	8
GCAGAGT	11270	0.0	26.104479	9
GTACATG	6425	0.0	25.684677	1
TACATGG	6420	0.0	24.516928	2
ACATGGG	6805	0.0	22.998417	3
GAGTACT	7970	0.0	22.05352	12-13
AGAGTAC	10335	0.0	21.122234	10-11
CAGAGTA	11275	0.0	20.778452	10-11
AGTACTT	8475	0.0	20.07398	12-13
GTACTTT	8730	0.0	19.83754	14-15
GTATTAG	1245	0.0	19.69306	1
ACACCGT	305	5.2150426E-9	18.490387	6
>>END_MODULE