FastQCFastQC Report
Wed 25 May 2016
SRR1033022_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033022_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3370210
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23850.035.6800731
GTATCAA35650.030.4640711
TCAACGC33000.030.3410224
ATCAACG34800.028.9067383
CAACGCA35150.028.351865
GTACATG86650.027.9430731
TACATGG87350.026.3692472
AACGCAG39500.025.344436
ACATGGG88350.024.9534653
TATCAAC44200.023.7163122
ACGCAGA47650.020.6150057
CGCAGAG48600.020.2273568
CATGGGG65700.020.1766154
GAGTACA62500.020.0847641
TATACCG2001.4067195E-518.8031675
GTATTAG17500.018.2686461
AGTACAT65000.017.2118722
GTACCGT2203.197367E-517.0909966
GTATAGA15800.016.663511
TATACTG20950.016.3798475
Produced by FastQC (version 0.11.5)