FastQCFastQC Report
Wed 25 May 2016
SRR1033022_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033022_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3370210
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47590.1412078179104566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41620.12349378822091205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41750.054.413341
GTATCAA79750.043.9109541
TCAACGC92400.037.639354
ATCAACG93400.037.1373753
CAACGCA95350.036.474845
TATCAAC98950.035.2309232
AACGCAG99350.034.9589926
ACGCAGA116150.029.942977
CGCAGAG116700.029.8022928
GTACATG84650.027.1711271
GCAGAGT132500.026.0711549
TACATGG86550.024.7657992
ACATGGG85000.024.274333
GAGTACT92400.021.74403212-13
AGAGTAC121200.021.69616910-11
CAGAGTA128100.021.48143810-11
CATGGGG60850.020.2359264
GTACTTT104550.019.37429814-15
AGTACTT101200.019.0173212-13
GTGGTAT19800.018.8051451
Produced by FastQC (version 0.11.5)