FastQCFastQC Report
Wed 25 May 2016
SRR1033021_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033021_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4425634
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68700.041.5401041
GGTATCA41800.039.703981
TCAACGC78550.035.010724
CAACGCA81100.034.0845645
ATCAACG81150.034.0048563
AACGCAG84750.032.6081286
TATCAAC90100.031.2531242
GTACATG213750.029.8036061
TACATGG209700.029.2328112
ACATGGG221850.027.1656423
ACGCAGA102700.026.9091537
CGCAGAG108850.025.321918
CATGGGG161050.025.1323914
GCAGAGT128750.021.2660399
GTATTAA17150.020.2863181
GAGTACA146000.019.707621
GTATATA16300.018.7482781
AGTACAT146950.018.5225332
CATGGGA56700.017.8257064
TACCGCG5550.017.797288
Produced by FastQC (version 0.11.5)