FastQCFastQC Report
Wed 25 May 2016
SRR1033021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033021_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4425634
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51450.050.567511
GTATCAA92400.044.275721
TCAACGC113850.035.254474
ATCAACG114500.035.0555273
CAACGCA115900.034.548635
AACGCAG122000.032.9367646
TATCAAC123750.032.5844422
GTACATG205200.027.9761411
ACGCAGA146250.027.5075917
TACATGG202650.027.2434882
CGCAGAG153300.026.2425658
ACATGGG215350.025.0772153
GTGGTAT22400.024.8285791
GCAGAGT176250.022.6121259
CATGGGG153450.021.9298444
GAGTACA145750.019.7260041
TGGTATC27000.019.3660452
CAGAGTA170650.018.69985210-11
AGTACAT147150.018.4072672
AGAGTAC163100.018.31202110-11
Produced by FastQC (version 0.11.5)