FastQCFastQC Report
Wed 25 May 2016
SRR1033020_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033020_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077095
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16850.034.8772471
GTATCAA27250.034.6786081
TCAACGC27100.033.655354
CAACGCA27650.032.985895
ATCAACG27700.032.926353
CCGCGTA600.00413880831.3687369
AACGCAG30100.030.2958776
GTACATG63800.029.5498831
TACATGG62950.028.3051262
TATCAAC35900.025.5365242
ACATGGG68100.025.1980763
ACGCAGA35900.024.484747
CGCAGAG37850.022.8716938
CATGGGG48650.020.873374
TATACTG12300.020.2578035
GAGTACA47550.019.9725251
AGTACAT47900.018.7464682
GCAGAGT44100.018.565179
GTGTAGG8750.017.7311941
TCTGTCG5100.017.5278958
Produced by FastQC (version 0.11.5)