FastQCFastQC Report
Wed 25 May 2016
SRR1033020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033020_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077095
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48170.23191043259937558No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38020.18304410727482373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28800.055.6558041
GTATCAA58400.045.690681
TCAACGC69700.037.2202764
ATCAACG70000.037.1296843
CAACGCA71450.036.4393275
TATCAAC73350.035.6539152
AACGCAG75300.034.5762256
ACGCAGA87550.029.3088877
CGCAGAG90050.028.5480778
GTACATG64600.028.0965021
TACATGG63850.027.2320182
GCAGAGT98900.025.7558759
GTGGTAT14650.025.7440221
TGGTATC14000.025.5799642
ACATGGG67500.024.9968263
GAGTACA47900.022.3416061
AGAGTAC92250.021.16790810-11
GAGTACT71050.021.16724812-13
CATGGGG43100.021.0452124
CAGAGTA97350.020.78310810-11
Produced by FastQC (version 0.11.5)