##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033020_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2077095
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72450224953601	34.0	31.0	34.0	31.0	34.0
2	32.72073352446566	34.0	31.0	34.0	31.0	34.0
3	32.81830248496097	34.0	31.0	34.0	31.0	34.0
4	36.30532594801875	37.0	37.0	37.0	35.0	37.0
5	36.292002532382966	37.0	37.0	37.0	35.0	37.0
6	36.3622092393463	37.0	37.0	37.0	35.0	37.0
7	36.31284365905267	37.0	37.0	37.0	35.0	37.0
8	36.32251726570041	37.0	37.0	37.0	35.0	37.0
9	38.129570867004155	39.0	39.0	39.0	37.0	39.0
10-11	38.088907825592955	39.0	39.0	39.0	36.0	39.0
12-13	38.06347302362194	39.0	39.0	39.0	35.0	39.0
14-15	39.5655795714688	41.0	40.0	41.0	37.0	41.0
16-17	39.56167483913832	41.0	40.0	41.0	37.0	41.0
18-19	39.58247745047771	41.0	40.0	41.0	37.0	41.0
20-21	39.57517975826816	41.0	40.0	41.0	37.0	41.0
22-23	39.50053440020798	41.0	39.0	41.0	36.5	41.0
24-25	39.44284589775624	41.0	39.0	41.0	36.0	41.0
26-27	39.330623539125554	41.0	39.0	41.0	36.0	41.0
28-29	39.27692113263957	41.0	39.0	41.0	36.0	41.0
30-31	39.26194155780068	41.0	39.0	41.0	36.0	41.0
32-33	39.21266793285815	41.0	39.0	41.0	35.0	41.0
34-35	39.12580599346684	41.0	39.0	41.0	35.0	41.0
36-37	39.029081000146846	41.0	39.0	41.0	35.0	41.0
38-39	38.93066783175541	41.0	39.0	41.0	35.0	41.0
40-41	38.8198787248537	41.0	39.0	41.0	35.0	41.0
42-43	38.69322515339934	41.0	39.0	41.0	35.0	41.0
44-45	38.51526049602931	41.0	38.0	41.0	35.0	41.0
46-47	38.28454018713636	41.0	38.0	41.0	34.5	41.0
48-49	38.05103112760851	40.0	38.0	41.0	34.0	41.0
50-51	37.90219489238576	40.0	38.0	41.0	34.0	41.0
52-53	37.76244875655664	40.0	37.5	41.0	33.0	41.0
54-55	37.60577272585029	40.0	37.0	41.0	33.0	41.0
56-57	37.44855338826582	40.0	37.0	41.0	33.0	41.0
58-59	37.264499697895374	40.0	36.5	41.0	33.0	41.0
60-61	37.068587137324	40.0	36.0	41.0	33.0	41.0
62-63	36.830130061456025	39.0	35.5	41.0	32.0	41.0
64-65	36.56260041066971	39.0	35.0	41.0	32.0	41.0
66-67	36.28603024897754	38.5	35.0	41.0	31.5	41.0
68-69	35.98138939239659	37.5	35.0	40.0	31.0	41.0
70-71	35.65724268750346	37.0	35.0	40.0	31.0	41.0
72-73	35.316055356158486	36.5	35.0	39.0	31.0	41.0
74-75	34.97355248556277	36.0	35.0	39.0	31.0	41.0
76-77	34.625868340157766	36.0	35.0	39.0	30.0	40.5
78-79	34.27854672029926	35.0	34.5	37.0	30.0	39.5
80-81	33.95569027897135	35.0	34.0	37.0	30.0	39.0
82-83	33.6625501000195	35.0	34.0	36.5	30.0	39.0
84-85	33.44419345287529	35.0	34.0	36.0	30.0	37.5
86-87	33.24391277240569	35.0	34.0	36.0	30.0	37.0
88-89	33.03708930020052	35.0	34.0	35.5	29.5	37.0
90-91	32.85767767001509	35.0	34.0	35.0	29.0	36.0
92-93	32.686276506370675	35.0	34.0	35.0	29.0	36.0
94-95	32.534134933645305	35.0	34.0	35.0	29.0	36.0
96-97	32.39512708855396	35.0	34.0	35.0	29.0	36.0
98-99	32.273978802125086	35.0	34.0	35.0	29.0	35.5
100	32.174296312879285	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12213931100481545
1101	2	0.0855753915895292
1101	3	0.13023644667228496
1101	4	0.06576925230562125
1101	5	0.09749294886074722
1101	6	0.11402428551203769
1101	7	0.1322333274075831
1101	8	0.1497050031739846
1101	9	0.20248575051586926
1101	10-11	0.1938016570528447
1101	12-13	0.18891109692357588
1101	14-15	0.21723766269401068
1101	16-17	0.24650593970654455
1101	18-19	0.25516255487898576
1101	20-21	0.2637392553372635
1101	22-23	0.3138664185555555
1101	24-25	0.3303732742027279
1101	26-27	0.3294543045513194
1101	28-29	0.2764857655126889
1101	30-31	0.29158531935079424
1101	32-33	0.2782356546056093
1101	34-35	0.31455996837225086
1101	36-37	0.3614693793935402
1101	38-39	0.2679660506834196
1101	40-41	0.30061849327920953
1101	42-43	0.324811071690597
1101	44-45	0.35032181221092884
1101	46-47	0.37003985354715496
1101	48-49	0.2799744185433326
1101	50-51	0.2778237192950499
1101	52-53	0.20781993637119456
1101	54-55	0.21919705591247407
1101	56-57	0.17807126228161962
1101	58-59	0.194418544096429
1101	60-61	0.20021573197293208
1101	62-63	0.18308877805002055
1101	64-65	0.19916596761540006
1101	66-67	0.17314607889796463
1101	68-69	0.1632288981603054
1101	70-71	0.17118231844212772
1101	72-73	0.20881272699689646
1101	74-75	0.14470237892201254
1101	76-77	0.19139205912536283
1101	78-79	0.2362032237118541
1101	80-81	0.23713122234788386
1101	82-83	0.21668592142382437
1101	84-85	0.25094239545568087
1101	86-87	0.2211127519493914
1101	88-89	0.2253172179291809
1101	90-91	0.2748730613245556
1101	92-93	0.20740402574228511
1101	94-95	0.21143238403706732
1101	96-97	0.28330369376307374
1101	98-99	0.3164182056569729
1101	100	0.23811100910072724
1102	1	0.17085751457646836
1102	2	0.1589364783295153
1102	3	0.18682184813453517
1102	4	0.16211461033303465
1102	5	0.13747947587548737
1102	6	0.11980056904184266
1102	7	0.15894173995322092
1102	8	0.17500739073903304
1102	9	0.21448640673663277
1102	10-11	0.2530419994242834
1102	12-13	0.22048130212643713
1102	14-15	0.2797307560209603
1102	16-17	0.3098057324463568
1102	18-19	0.28227264374285
1102	20-21	0.21784427869713596
1102	22-23	0.26727417577711066
1102	24-25	0.3032983580647368
1102	26-27	0.27504607587631114
1102	28-29	0.2794520197765138
1102	30-31	0.26771914090092963
1102	32-33	0.24703978318901676
1102	34-35	0.24077333585196925
1102	36-37	0.25654004499917704
1102	38-39	0.27629636665574253
1102	40-41	0.31824981668174956
1102	42-43	0.24381611420918148
1102	44-45	0.29582492553391404
1102	46-47	0.20041745707084146
1102	48-49	0.21518467882529535
1102	50-51	0.23882417559328672
1102	52-53	0.20010302768628208
1102	54-55	0.08449037813367255
1102	56-57	0.11326081043775815
1102	58-59	0.11959971520801815
1102	60-61	0.12625740350715375
1102	62-63	0.1272054418771873
1102	64-65	0.1287111457504153
1102	66-67	0.216748639977574
1102	68-69	0.26414355321437455
1102	70-71	0.24076779934296155
1102	72-73	0.26891249629665026
1102	74-75	0.30152266094285807
1102	76-77	0.2763057760716805
1102	78-79	0.2554108156657975
1102	80-81	0.250651946534731
1102	82-83	0.3076442954815306
1102	84-85	0.2431319664709335
1102	86-87	0.2916058407297655
1102	88-89	0.34551126276360833
1102	90-91	0.2523104207204767
1102	92-93	0.30860094665745663
1102	94-95	0.27439118446145727
1102	96-97	0.3071859114811133
1102	98-99	0.33341420985849624
1102	100	0.38972882188256364
1103	1	0.11282576260875032
1103	2	0.08215918734775585
1103	3	0.10677113325390764
1103	4	0.07537558985082171
1103	5	0.10818074530975963
1103	6	0.11794282164569125
1103	7	0.1399328135874427
1103	8	0.10439712297735326
1103	9	0.2164599811007193
1103	10-11	0.20145920987151555
1103	12-13	0.19558619327105475
1103	14-15	0.23958584903892444
1103	16-17	0.23784496373608732
1103	18-19	0.2624407844673655
1103	20-21	0.2525814454596471
1103	22-23	0.20182730093272738
1103	24-25	0.19563493341402705
1103	26-27	0.1817325018832605
1103	28-29	0.24028108455856056
1103	30-31	0.17893996690709457
1103	32-33	0.21658052498933955
1103	34-35	0.2770150326816463
1103	36-37	0.27734392321315937
1103	38-39	0.26801431126774844
1103	40-41	0.1921633133429097
1103	42-43	0.16853908148861052
1103	44-45	0.21883361707241988
1103	46-47	0.19729568228435568
1103	48-49	0.17648077405287665
1103	50-51	0.19737655086387207
1103	52-53	0.19127765960307386
1103	54-55	0.18573553634671924
1103	56-57	0.17702026089310863
1103	58-59	0.1611789115616986
1103	60-61	0.13576223284484712
1103	62-63	0.16153422398775064
1103	64-65	0.14128484050619505
1103	66-67	0.1681173632299675
1103	68-69	0.19101706967718712
1103	70-71	0.11165307376865741
1103	72-73	0.18482889143919579
1103	74-75	0.204114011683032
1103	76-77	0.1936790401317836
1103	78-79	0.16325307032010983
1103	80-81	0.21554831984468592
1103	82-83	0.16131620564764404
1103	84-85	0.20216601837898907
1103	86-87	0.24451485516797788
1103	88-89	0.2575614293884456
1103	90-91	0.226490770998808
1103	92-93	0.28172414700662074
1103	94-95	0.22697322266458997
1103	96-97	0.2547381422426014
1103	98-99	0.290573732275476
1103	100	0.24507802615494967
1104	1	0.0912203684710704
1104	2	0.08146341530088819
1104	3	0.07905079460149267
1104	4	0.09247565097471977
1104	5	0.11189369707390284
1104	6	0.11439591694880846
1104	7	0.12111886804902383
1104	8	0.158675703045013
1104	9	0.15713217678637648
1104	10-11	0.18127276339951237
1104	12-13	0.19993267846105311
1104	14-15	0.17817316703740005
1104	16-17	0.23610344351597234
1104	18-19	0.2637544141680124
1104	20-21	0.24769025621894514
1104	22-23	0.27744281302652496
1104	24-25	0.26788236072953
1104	26-27	0.3154716867616969
1104	28-29	0.33017977959118383
1104	30-31	0.29463773151877604
1104	32-33	0.27921243370850846
1104	34-35	0.22468709924538643
1104	36-37	0.21755339333025603
1104	38-39	0.19400724950659765
1104	40-41	0.2449572236189681
1104	42-43	0.21817856195404772
1104	44-45	0.2194491523895934
1104	46-47	0.26115262356267976
1104	48-49	0.20310798886529824
1104	50-51	0.24159500099215592
1104	52-53	0.27269076891452926
1104	54-55	0.325941848086039
1104	56-57	0.31150851544349223
1104	58-59	0.300049763636288
1104	60-61	0.3441184411937428
1104	62-63	0.19771030633594222
1104	64-65	0.2555102437102761
1104	66-67	0.22626762676872403
1104	68-69	0.22241970525055166
1104	70-71	0.2711850579173145
1104	72-73	0.20332386404491132
1104	74-75	0.11321078995190703
1104	76-77	0.184090576756887
1104	78-79	0.15345280984683995
1104	80-81	0.2237059916372175
1104	82-83	0.12325461820982042
1104	84-85	0.2002708755924445
1104	86-87	0.2442871500638546
1104	88-89	0.27547094093603874
1104	90-91	0.30873237389410946
1104	92-93	0.2865475240184594
1104	94-95	0.34140275925624763
1104	96-97	0.29960429205003436
1104	98-99	0.3078893062363264
1104	100	0.2976471342476472
1105	1	0.05590701535729892
1105	2	0.0478119703518729
1105	3	0.08237944078300075
1105	4	0.030613699121758486
1105	5	0.05411878793549363
1105	6	0.08496466495238053
1105	7	0.03572543818451379
1105	8	0.07621662780799454
1105	9	0.10279249988152372
1105	10-11	0.09708295460676197
1105	12-13	0.10761225290718102
1105	14-15	0.1545416342509398
1105	16-17	0.1774425309593468
1105	18-19	0.14805794402143846
1105	20-21	0.1493802653407812
1105	22-23	0.19095245791165638
1105	24-25	0.16449235347377567
1105	26-27	0.18544219173369214
1105	28-29	0.12359762435754362
1105	30-31	0.1322456485243535
1105	32-33	0.1731996521638166
1105	34-35	0.2071748791709922
1105	36-37	0.28418542776271494
1105	38-39	0.2478361146779875
1105	40-41	0.18766963557021654
1105	42-43	0.1517830256154511
1105	44-45	0.11520173663482325
1105	46-47	0.09375900928564107
1105	48-49	0.12511466064722043
1105	50-51	0.17394974044908906
1105	52-53	0.13388043830018148
1105	54-55	0.1335570428004189
1105	56-57	0.16897744248089452
1105	58-59	0.21336176878227064
1105	60-61	0.1821098374710175
1105	62-63	0.14189593291593638
1105	64-65	0.098864202707027
1105	66-67	0.09655690197259759
1105	68-69	0.13762473795633667
1105	70-71	0.16895004390525514
1105	72-73	0.20935925807850708
1105	74-75	0.15046892688040003
1105	76-77	0.1088947756545835
1105	78-79	0.19981862064177847
1105	80-81	0.2615714824544568
1105	82-83	0.31492054393328317
1105	84-85	0.2810800075253468
1105	86-87	0.2142961840824782
1105	88-89	0.1960885363064051
1105	90-91	0.20568715220939282
1105	92-93	0.18842057355408315
1105	94-95	0.18161758887773516
1105	96-97	0.20488639382599416
1105	98-99	0.22237040998922453
1105	100	0.23189802969123008
1106	1	0.05049968309372588
1106	2	-0.02764520883825128
1106	3	0.011291778817842157
1106	4	0.02990054814603127
1106	5	0.017822477013616833
1106	6	0.04891958979776945
1106	7	0.007235152758696017
1106	8	0.05211688486039634
1106	9	0.04802705152333431
1106	10-11	0.06807264102631905
1106	12-13	0.10051462902761443
1106	14-15	0.1858576283318527
1106	16-17	0.10450694134240734
1106	18-19	0.08739859421405072
1106	20-21	0.11987740585297502
1106	22-23	0.12260683049728271
1106	24-25	0.11000508949425125
1106	26-27	0.018356531970319168
1106	28-29	0.08900339840437965
1106	30-31	0.059518123532853906
1106	32-33	0.08276251366876863
1106	34-35	0.08094534210544424
1106	36-37	0.029840057560832633
1106	38-39	0.08335523831981817
1106	40-41	0.08888728589678152
1106	42-43	0.08770765915435419
1106	44-45	0.1120935745141125
1106	46-47	0.11871529116704949
1106	48-49	0.16134026481641683
1106	50-51	0.22342194789256808
1106	52-53	0.231179173529803
1106	54-55	0.21007509009870518
1106	56-57	0.16170233131120426
1106	58-59	0.11321016283181251
1106	60-61	0.17399510362457704
1106	62-63	0.1062233889616877
1106	64-65	0.12554941144136933
1106	66-67	0.1454978215446019
1106	68-69	0.15451329931138247
1106	70-71	0.13840894247015
1106	72-73	0.1165461058754147
1106	74-75	0.10162929139639942
1106	76-77	0.0904469302894455
1106	78-79	0.07984575068937261
1106	80-81	0.11344350376573686
1106	82-83	0.14586539426709777
1106	84-85	0.09592148832219038
1106	86-87	0.04241479212532795
1106	88-89	0.11819072010447229
1106	90-91	0.14752996762953785
1106	92-93	0.0929840674175395
1106	94-95	0.12688223758257067
1106	96-97	0.12305690862984875
1106	98-99	0.08061970012011699
1106	100	0.021886166965856546
1107	1	-0.03282148669523366
1107	2	-0.015212901532436263
1107	3	-0.008888569197949892
1107	4	-0.015989558102354806
1107	5	1.6933661945728318E-4
1107	6	0.019354673269837974
1107	7	0.05347382164618608
1107	8	0.005942151673735907
1107	9	0.060574689787884495
1107	10-11	0.0800187491923694
1107	12-13	0.03348961063839084
1107	14-15	0.08083419415005721
1107	16-17	0.09953074968167641
1107	18-19	0.08795021951712556
1107	20-21	0.05366576881532836
1107	22-23	0.13609546453879062
1107	24-25	0.10200140877146424
1107	26-27	0.1574143684931073
1107	28-29	0.13030323998363258
1107	30-31	0.11465757736984017
1107	32-33	0.015100862219895816
1107	34-35	0.13736926622794954
1107	36-37	0.13057471165635803
1107	38-39	0.08468581015168297
1107	40-41	0.03412666650579865
1107	42-43	0.06286067311618382
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2216	28-29	-0.38826337585474135
2216	30-31	-0.3223729974295253
2216	32-33	-0.3253963030916509
2216	34-35	-0.36675003471597734
2216	36-37	-0.4005122179104106
2216	38-39	-0.33122442295494636
2216	40-41	-0.27724371212099186
2216	42-43	-0.2776369868478312
2216	44-45	-0.2900713598049194
2216	46-47	-0.24405338585505376
2216	48-49	-0.30886763054151345
2216	50-51	-0.31750033111203635
2216	52-53	-0.27952165135324947
2216	54-55	-0.2815062751021884
2216	56-57	-0.3025623287289392
2216	58-59	-0.33242070421966474
2216	60-61	-0.31046526747388725
2216	62-63	-0.3200770313582808
2216	64-65	-0.3704593991117875
2216	66-67	-0.35908979320439016
2216	68-69	-0.3333794375841421
2216	70-71	-0.38575685506686597
2216	72-73	-0.41228374605081086
2216	74-75	-0.45983014042814574
2216	76-77	-0.35876926710405144
2216	78-79	-0.4473390928945804
2216	80-81	-0.4559961753395356
2216	82-83	-0.45769885015967304
2216	84-85	-0.4802002005155259
2216	86-87	-0.4412284890988758
2216	88-89	-0.582585434274776
2216	90-91	-0.6784995076321536
2216	92-93	-0.6171319622435973
2216	94-95	-0.49127259903014675
2216	96-97	-0.5972235894649955
2216	98-99	-0.6670082836264264
2216	100	-0.7231840944074079
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	11.0
3	5.0
4	6.0
5	12.0
6	40.0
7	130.0
8	278.0
9	504.0
10	987.0
11	1464.0
12	2350.0
13	3659.0
14	4358.0
15	5700.0
16	7195.0
17	10428.0
18	12766.0
19	6730.0
20	4389.0
21	4042.0
22	4149.0
23	4447.0
24	4965.0
25	6131.0
26	7610.0
27	9997.0
28	12984.0
29	16236.0
30	21117.0
31	27163.0
32	34703.0
33	45527.0
34	63188.0
35	98512.0
36	180728.0
37	402363.0
38	717553.0
39	353289.0
40	1379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.32778780703495	19.578662022167975	13.375925030392825	23.717625140404248
2	15.753889752500788	20.07217982989768	40.204927783974014	23.96900263362752
3	18.3387673274763	25.336583458831957	29.96936036143604	26.355288852255704
4	12.252990307379141	16.70017582680001	38.75623241491273	32.29060145090812
5	12.477083301237117	37.74004760510869	35.72650749510854	14.056361598545655
6	30.746210452579202	38.25602584378663	17.455677280047375	13.542086423586788
7	27.19403782686878	32.706737053432796	22.352757095847807	17.746468023850618
8	25.40761353624542	35.89093395086395	20.11602771184686	18.585424801043768
9	25.35050154181682	16.137682677008033	21.213232904609562	37.29858287656559
10-11	24.031091724921524	26.485330367631484	28.672511410249	20.811066497197988
12-13	25.01784944838825	23.883548898822635	29.051174837934713	22.0474268148544
14-15	22.11889127630903	25.68936555981418	26.66744018437324	25.524302979503545
16-17	21.067911034294333	29.190900260821316	27.201942137868194	22.539246567016153
18-19	20.899178848809196	27.531939006799917	30.172564036890176	21.396318107500708
20-21	22.11763165268399	26.894931091226493	29.86196098972892	21.1254762663606
22-23	21.693264724658583	26.718193784200427	29.932492222306745	21.656049268834245
24-25	21.4711727707957	26.884944325259113	29.911259354550463	21.732623549394724
26-27	21.283230938624317	26.847570137687626	30.02944499837032	21.83975392531774
28-29	21.323058057921916	26.895976316860686	30.044949916698343	21.73601570851906
30-31	21.52632141474716	27.342650205190715	29.620305696618153	21.51072268344397
32-33	21.20735931673804	27.086339334503233	30.124958174758497	21.581343174000224
34-35	21.406146565275062	26.820583555398287	30.07019418948098	21.703075689845676
36-37	21.78937410181046	27.190739951711407	29.44504223446689	21.574843712011248
38-39	21.582763426805226	27.36261461319776	29.544700651631246	21.50992130836577
40-41	21.367839242672513	27.1305793407952	29.682390320897927	21.81919109563436
42-43	21.414331072964888	27.083258108078834	29.59075054342724	21.911660275529044
44-45	21.58293193137531	27.316227712261597	29.36740977182074	21.733430584542354
46-47	21.728471735765574	27.57252316336037	28.957221504071796	21.741783596802264
48-49	21.81436092234587	27.501414234784637	28.664938291219226	22.01928655165026
50-51	21.797606753663167	27.495829511890406	28.48755112308296	22.219012611363468
52-53	21.808968776103164	27.593610306702388	28.51494515176244	22.08247576543201
54-55	21.921746477652686	27.95445080749797	28.315315380374994	21.80848733447435
56-57	21.714028486901178	27.613951215519748	28.4514670730034	22.22055322457567
58-59	21.575686234861667	27.21445095193046	28.882477691198527	22.327385122009343
60-61	21.904149786119557	27.629549924293308	28.409918660436812	22.05638162915033
62-63	21.698935291837877	27.56749209833927	28.530784581350392	22.202788028472458
64-65	21.678931392160685	27.336761197730485	28.64702866262737	22.33727874748146
66-67	21.582455685092803	27.47465269394339	28.453110823797378	22.489780797166425
68-69	21.96259095051685	27.60305157549669	28.348661661910818	22.085695812075638
70-71	22.021723132962894	27.367146752350457	28.352658003764393	22.258472110922252
72-73	21.83874732317552	27.537326295274923	28.394897086690595	22.229029294858957
74-75	21.89499413483312	27.58797329056203	28.27371516977931	22.24331740482554
76-77	21.892450754539393	27.649577896051937	28.12798644260373	22.32998490680494
78-79	21.966689053702407	27.803205919806267	28.128058658848055	22.102046367643272
80-81	21.89160823168897	27.551628596669868	28.244013875147743	22.31274929649342
82-83	21.73493351769347	27.58907396583881	28.43629128002873	22.239701236438986
84-85	21.731264097212694	27.535668806674707	28.25520739301765	22.477859703094946
86-87	21.886404208977588	27.644596944562664	28.144793373787486	22.324205472672265
88-89	21.578552956211997	27.634185450796238	28.21607079107471	22.571190801917055
90-91	21.56730769925175	27.52114247225438	28.25220704757902	22.659342780914855
92-93	21.662336874519156	27.67134374088264	28.149273837046394	22.517045547551806
94-95	21.42650981532519	27.442445140024464	28.328110063943022	22.802934980707324
96-97	21.462640334351903	27.42876703161582	28.328534158075737	22.78005847595654
98-99	21.438587141118106	27.54919313935555	28.17788414435845	22.8343355751679
100	21.475474159824177	27.5109227069537	28.095681709310362	22.91792142391176
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	30.5
2	135.5
3	243.5
4	166.5
5	120.0
6	315.0
7	698.0
8	1245.5
9	1480.0
10	1084.0
11	760.5
12	817.5
13	958.0
14	1094.0
15	1260.5
16	1462.5
17	1655.0
18	1968.0
19	2323.5
20	2769.0
21	3433.0
22	4405.5
23	5981.0
24	7823.5
25	10123.0
26	13208.5
27	17214.5
28	22252.0
29	27634.0
30	32889.5
31	38684.0
32	44464.5
33	49671.5
34	55066.0
35	59987.0
36	63913.0
37	67680.0
38	70825.0
39	72224.5
40	73416.0
41	74506.0
42	74209.5
43	74177.0
44	72866.5
45	70634.0
46	69991.5
47	68273.0
48	66051.5
49	64657.0
50	63379.0
51	61470.0
52	57925.5
53	54693.0
54	52225.0
55	49726.5
56	46233.0
57	41799.5
58	38235.0
59	35046.5
60	31491.5
61	27587.0
62	23854.0
63	20142.0
64	16469.5
65	13497.5
66	10943.5
67	8446.0
68	6536.5
69	5247.0
70	3950.0
71	2875.0
72	2190.0
73	1755.5
74	1401.0
75	997.0
76	677.0
77	484.5
78	357.5
79	264.5
80	179.0
81	80.0
82	35.5
83	18.0
84	10.5
85	6.0
86	2.0
87	0.5
88	1.5
89	2.5
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04559252224862127
2	0.24303173422496321
3	0.002262775655422597
4	0.0024553523069479246
5	3.37009140169323E-4
6	0.0
7	0.0
8	2.407208144066593E-4
9	0.0
10-11	7.221624432199779E-4
12-13	0.0
14-15	1.9257665152532744E-4
16-17	1.2036040720332966E-4
18-19	5.295857916946505E-4
20-21	4.814416288133186E-5
22-23	4.814416288133186E-5
24-25	0.0014924690493212877
26-27	9.628832576266372E-5
28-29	2.6479289584732527E-4
30-31	1.4443248864399558E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	7.221624432199779E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	2.407208144066593E-5
68-69	1.9257665152532744E-4
70-71	2.407208144066593E-5
72-73	3.37009140169323E-4
74-75	7.221624432199779E-5
76-77	0.0
78-79	0.0
80-81	0.0
82-83	4.814416288133186E-5
84-85	0.0
86-87	5.295857916946505E-4
88-89	0.0025757127141512546
90-91	0.0026720010399139183
92-93	6.258741174573142E-4
94-95	5.536578731353164E-4
96-97	9.628832576266372E-5
98-99	7.221624432199779E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2077095.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.07429116235191
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.83342312918083	55.83511404874627
2	12.44417546228324	17.191451983142954
3	3.2682843887302866	6.772632824055753
4	1.298393870066454	3.587425448975326
5	0.6292713862987034	2.173323747866673
6	0.379834779839799	1.5742090905745267
7	0.2310892430599701	1.117362796172832
8	0.1754941445512175	0.9697706910414936
9	0.11844590039631983	0.7363409949866097
>10	0.5836004254297782	7.08097496314818
>50	0.028829707351148176	1.3373242851011753
>100	0.008668870859015377	0.969491088665168
>500	2.094394085061888E-4	0.0833292212199914
>1k	2.7925254467473594E-4	0.571248816302916
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4817	0.23191043259937558	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3802	0.18304410727482373	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	4.814416288133186E-5	0.0
8	0.0	0.0	0.0	4.814416288133186E-5	0.0
9	0.0	0.0	0.0	4.814416288133186E-5	0.0
10-11	0.0	0.0	0.0	1.2036040720332964E-4	0.0
12-13	0.0	0.0	0.0	1.685045700846615E-4	0.0
14-15	0.0	2.407208144066593E-5	0.0	1.9257665152532744E-4	0.0
16-17	0.0	4.814416288133186E-5	0.0	1.9257665152532744E-4	0.0
18-19	0.0	4.814416288133186E-5	0.0	1.9257665152532744E-4	0.0
20-21	0.0	4.814416288133186E-5	0.0	1.9257665152532744E-4	0.0
22-23	0.0	4.814416288133186E-5	0.0	2.1664873296599338E-4	0.0
24-25	0.0	4.814416288133186E-5	0.0	2.407208144066593E-4	0.0
26-27	0.0	4.814416288133186E-5	0.0	3.129370587286571E-4	0.0
28-29	0.0	4.814416288133186E-5	0.0	7.462345246606438E-4	0.0
30-31	2.407208144066593E-5	4.814416288133186E-5	0.0	0.003177514750167903	0.0
32-33	4.814416288133186E-5	4.814416288133186E-5	0.0	0.0086900214000804	0.0
34-35	4.814416288133186E-5	4.814416288133186E-5	0.0	0.015815357506517518	0.0
36-37	4.814416288133186E-5	7.221624432199779E-5	0.0	0.03129370587286571	0.0
38-39	4.814416288133186E-5	9.628832576266372E-5	0.0	0.05794150002768289	0.0
40-41	4.814416288133186E-5	9.628832576266372E-5	0.0	0.09005365666953125	0.0
42-43	4.814416288133186E-5	9.628832576266372E-5	0.0	0.1230083361618029	0.0
44-45	4.814416288133186E-5	9.628832576266372E-5	0.0	0.16019970198763178	0.0
46-47	4.814416288133186E-5	9.628832576266372E-5	0.0	0.20742912577421832	0.0
48-49	4.814416288133186E-5	9.628832576266372E-5	0.0	0.2630356339021566	0.0
50-51	9.628832576266372E-5	9.628832576266372E-5	0.0	0.32764510048890394	0.0
52-53	9.628832576266372E-5	9.628832576266372E-5	0.0	0.39153240463243133	0.0
54-55	9.628832576266372E-5	9.628832576266372E-5	0.0	0.45869351185188934	0.0
56-57	9.628832576266372E-5	9.628832576266372E-5	0.0	0.536398190742359	0.0
58-59	9.628832576266372E-5	9.628832576266372E-5	0.0	0.6302070921166341	0.0
60-61	9.628832576266372E-5	9.628832576266372E-5	0.0	0.7326819428095489	0.0
62-63	9.628832576266372E-5	9.628832576266372E-5	0.0	0.8362159650858532	0.0
64-65	9.628832576266372E-5	9.628832576266372E-5	0.0	0.9476456300746956	0.0
66-67	9.628832576266372E-5	9.628832576266372E-5	0.0	1.0734703997650565	0.0
68-69	9.628832576266372E-5	9.628832576266372E-5	0.0	1.2178788163276115	0.0
70-71	9.628832576266372E-5	9.628832576266372E-5	0.0	1.376658265510244	0.0
72-73	9.628832576266372E-5	9.628832576266372E-5	0.0	1.5536362082620196	0.0
74-75	9.628832576266372E-5	9.628832576266372E-5	0.0	1.727219987530662	0.0
76-77	9.628832576266372E-5	1.4443248864399558E-4	0.0	1.9087716257561644	0.0
78-79	9.628832576266372E-5	1.4443248864399558E-4	0.0	2.122868718089447	0.0
80-81	9.628832576266372E-5	1.4443248864399558E-4	0.0	2.3669596238977997	0.0
82-83	9.628832576266372E-5	1.4443248864399558E-4	0.0	2.6236883724625017	0.0
84-85	9.628832576266372E-5	1.4443248864399558E-4	0.0	2.8822225271352537	0.0
86-87	9.628832576266372E-5	1.4443248864399558E-4	0.0	3.149542991533849	0.0
88	9.628832576266372E-5	1.4443248864399558E-4	0.0	3.371343149928145	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2880	0.0	55.655804	1
GTATCAA	5840	0.0	45.69068	1
TCAACGC	6970	0.0	37.220276	4
ATCAACG	7000	0.0	37.129684	3
CAACGCA	7145	0.0	36.439327	5
TATCAAC	7335	0.0	35.653915	2
AACGCAG	7530	0.0	34.576225	6
ACGCAGA	8755	0.0	29.308887	7
CGCAGAG	9005	0.0	28.548077	8
GTACATG	6460	0.0	28.096502	1
TACATGG	6385	0.0	27.232018	2
GCAGAGT	9890	0.0	25.755875	9
GTGGTAT	1465	0.0	25.744022	1
TGGTATC	1400	0.0	25.579964	2
ACATGGG	6750	0.0	24.996826	3
GAGTACA	4790	0.0	22.341606	1
AGAGTAC	9225	0.0	21.167908	10-11
GAGTACT	7105	0.0	21.167248	12-13
CATGGGG	4310	0.0	21.045212	4
CAGAGTA	9735	0.0	20.783108	10-11
>>END_MODULE