FastQCFastQC Report
Wed 25 May 2016
SRR1033019_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033019_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4099788
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46550.036.1521261
TCAACGC46700.034.1218154
GGTATCA30500.034.0614361
ATCAACG48850.032.523813
CAACGCA49750.032.408655
AACGCAG51100.031.5489866
GTACATG111050.027.9803681
TATCAAC59450.027.594522
TACATGG111700.026.7641352
ACGCAGA62850.025.0534557
ACATGGG113350.025.047523
CGCAGAG63500.024.451688
CATGGGG81250.021.17424
GAGTACA78350.018.6591511
GCAGAGT80050.018.5753359
GTATACG3901.8189894E-1118.0799471
TATACTG22200.016.7276155
AGTACAT80500.016.4665472
AGAGTAC67150.016.33413310-11
CTGTCGC12350.016.0026999
Produced by FastQC (version 0.11.5)