FastQCFastQC Report
Wed 25 May 2016
SRR1033019_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033019_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4099788
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57950.14134877218041517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49490.1207135588474331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50300.053.132181
GTATCAA96000.044.139871
ATCAACG115600.036.0634353
TCAACGC116100.035.865454
CAACGCA119100.034.883125
TATCAAC124800.033.67332
AACGCAG123700.033.6619156
ACGCAGA143600.028.8007017
CGCAGAG145700.028.224318
GTACATG105200.027.062241
TACATGG107250.024.9069882
GCAGAGT165650.024.6832669
ATACGCG500.001759889523.49932786-87
ACATGGG109450.023.317683
AGAGTAC151350.021.47259510-11
GAGTACT113050.020.7444612-13
CAGAGTA161200.020.20426210-11
AGTACTT120950.019.71979112-13
CATGGGG80300.019.372574
GTACTTT127800.018.22164714-15
Produced by FastQC (version 0.11.5)