##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033019_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4099788
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7290098902675	34.0	31.0	34.0	31.0	34.0
2	32.72739395305318	34.0	31.0	34.0	31.0	34.0
3	32.828725046270684	34.0	31.0	34.0	31.0	34.0
4	36.314274055146264	37.0	37.0	37.0	35.0	37.0
5	36.29946694804707	37.0	37.0	37.0	35.0	37.0
6	36.36443933198497	37.0	37.0	37.0	35.0	37.0
7	36.31547923941433	37.0	37.0	37.0	35.0	37.0
8	36.322538140996556	37.0	37.0	37.0	35.0	37.0
9	38.134195719388416	39.0	39.0	39.0	37.0	39.0
10-11	38.091973658150124	39.0	39.0	39.0	36.0	39.0
12-13	38.06878172725029	39.0	39.0	39.0	35.0	39.0
14-15	39.570627554400374	41.0	40.0	41.0	37.0	41.0
16-17	39.5692346775004	41.0	40.0	41.0	37.0	41.0
18-19	39.58536599941265	41.0	40.0	41.0	37.0	41.0
20-21	39.5763891937827	41.0	40.0	41.0	37.0	41.0
22-23	39.505681879160576	41.0	39.0	41.0	36.5	41.0
24-25	39.45012595773245	41.0	39.0	41.0	36.0	41.0
26-27	39.34061578306	41.0	39.0	41.0	36.0	41.0
28-29	39.28653884054492	41.0	39.0	41.0	36.0	41.0
30-31	39.282688153631355	41.0	39.0	41.0	36.0	41.0
32-33	39.23919139233541	41.0	39.0	41.0	35.5	41.0
34-35	39.156524922752105	41.0	39.0	41.0	35.0	41.0
36-37	39.065065925360045	41.0	39.0	41.0	35.0	41.0
38-39	38.97025224231106	41.0	39.0	41.0	35.0	41.0
40-41	38.86579306051922	41.0	39.0	41.0	35.0	41.0
42-43	38.747771714049605	41.0	39.0	41.0	35.0	41.0
44-45	38.58055416523976	41.0	38.5	41.0	35.0	41.0
46-47	38.384028027790706	41.0	38.0	41.0	34.5	41.0
48-49	38.18082264253664	40.0	38.0	41.0	34.0	41.0
50-51	38.04357725326285	40.0	38.0	41.0	34.0	41.0
52-53	37.912654142116615	40.0	38.0	41.0	34.0	41.0
54-55	37.762342833336746	40.0	37.5	41.0	33.0	41.0
56-57	37.60966542660255	40.0	37.0	41.0	33.0	41.0
58-59	37.43156743714553	40.0	37.0	41.0	33.0	41.0
60-61	37.23861331366402	40.0	36.0	41.0	33.0	41.0
62-63	37.006426795726995	39.0	36.0	41.0	32.5	41.0
64-65	36.73929481231713	39.0	35.0	41.0	32.0	41.0
66-67	36.46042283161958	39.0	35.0	41.0	32.0	41.0
68-69	36.153294023983676	38.0	35.0	40.5	31.5	41.0
70-71	35.823231957359745	37.0	35.0	40.0	31.0	41.0
72-73	35.47876219453299	37.0	35.0	39.0	31.0	41.0
74-75	35.13318347192587	36.0	35.0	39.0	31.0	41.0
76-77	34.77848915602465	36.0	35.0	39.0	31.0	40.5
78-79	34.41430008088223	35.0	35.0	37.5	30.0	39.5
80-81	34.08233645251901	35.0	34.0	37.0	30.0	39.0
82-83	33.78158809675037	35.0	34.0	36.5	30.0	39.0
84-85	33.557932141857094	35.0	34.0	36.0	30.0	37.5
86-87	33.349118661745436	35.0	34.0	36.0	30.0	37.0
88-89	33.141430361765046	35.0	34.0	35.5	30.0	37.0
90-91	32.96078833832384	35.0	34.0	35.0	30.0	36.0
92-93	32.78942386777072	35.0	34.0	35.0	29.0	36.0
94-95	32.63720343588498	35.0	34.0	35.0	29.0	36.0
96-97	32.49836735460468	35.0	34.0	35.0	29.0	36.0
98-99	32.376132741497855	35.0	34.0	35.0	29.0	35.5
100	32.27390684591496	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.10237625391726368
1101	2	0.09891484446362853
1101	3	0.10747372118589738
1101	4	0.08819363437226002
1101	5	0.12064330601713635
1101	6	0.10545336853424203
1101	7	0.14445603780683314
1101	8	0.13495405978441255
1101	9	0.20440698341192132
1101	10-11	0.19956994179734977
1101	12-13	0.1793606015221627
1101	14-15	0.2591881548406363
1101	16-17	0.25788995179921415
1101	18-19	0.2740903947600728
1101	20-21	0.2633924375370924
1101	22-23	0.2258061367069999
1101	24-25	0.2770294498928152
1101	26-27	0.3298383098041242
1101	28-29	0.3323956416798879
1101	30-31	0.2907964585103926
1101	32-33	0.2693571583828529
1101	34-35	0.23984240364735854
1101	36-37	0.24618940136627998
1101	38-39	0.22520200285459424
1101	40-41	0.2889919129204017
1101	42-43	0.30056949799791965
1101	44-45	0.3315859411033557
1101	46-47	0.32214655723506525
1101	48-49	0.29767024331456327
1101	50-51	0.2907119930729962
1101	52-53	0.3000942190530793
1101	54-55	0.27915167270325014
1101	56-57	0.287492330341081
1101	58-59	0.24066418042031756
1101	60-61	0.2362923803481891
1101	62-63	0.21494898350844238
1101	64-65	0.2212908759271741
1101	66-67	0.2588775699980559
1101	68-69	0.23668030592046563
1101	70-71	0.2792292760843509
1101	72-73	0.2511164070857461
1101	74-75	0.2523602162717111
1101	76-77	0.21856145807679894
1101	78-79	0.22898883467203035
1101	80-81	0.27171974838883983
1101	82-83	0.2325389585073907
1101	84-85	0.30069704543888065
1101	86-87	0.30721248076280006
1101	88-89	0.35992146041655815
1101	90-91	0.3245870922008436
1101	92-93	0.2822857287384579
1101	94-95	0.323468890496045
1101	96-97	0.3946049653508581
1101	98-99	0.3996760380999831
1101	100	0.3683582906476275
1102	1	0.09985543120244245
1102	2	0.04664433209611474
1102	3	0.11397501521170028
1102	4	0.07188807542920017
1102	5	0.07687229698970555
1102	6	0.10620393476824574
1102	7	0.10223556666262112
1102	8	0.12198440867628335
1102	9	0.17280721730591608
1102	10-11	0.17502311140489013
1102	12-13	0.17304270164838442
1102	14-15	0.25911138883156326
1102	16-17	0.22097868663492903
1102	18-19	0.22735693697512005
1102	20-21	0.20330336186655273
1102	22-23	0.22210917326388824
1102	24-25	0.24326350648390616
1102	26-27	0.2442301906390938
1102	28-29	0.27453549027418234
1102	30-31	0.2468824968944361
1102	32-33	0.2680935158695874
1102	34-35	0.20990558837878837
1102	36-37	0.17795333103548217
1102	38-39	0.2180136712879701
1102	40-41	0.21594006537676336
1102	42-43	0.20043175695109028
1102	44-45	0.28499444571400545
1102	46-47	0.2975033654367749
1102	48-49	0.2614052614350939
1102	50-51	0.2326644867435803
1102	52-53	0.26494330844040803
1102	54-55	0.2611296925050226
1102	56-57	0.23459174258340454
1102	58-59	0.24458774169605846
1102	60-61	0.21357923089888686
1102	62-63	0.2186928508267627
1102	64-65	0.2571360160082037
1102	66-67	0.23368680151453702
1102	68-69	0.21919751182534952
1102	70-71	0.2269816945002745
1102	72-73	0.22465798320187247
1102	74-75	0.2062003928804259
1102	76-77	0.12583499835002243
1102	78-79	0.23129895475637596
1102	80-81	0.2136811537957115
1102	82-83	0.17293518159888777
1102	84-85	0.21493346598528262
1102	86-87	0.241014488040193
1102	88-89	0.1718553697294709
1102	90-91	0.11796864271788365
1102	92-93	0.16398951721657795
1102	94-95	0.24482182756459991
1102	96-97	0.2227478637393503
1102	98-99	0.2446880715497457
1102	100	0.26545494077286236
1103	1	0.11755710372298012
1103	2	0.08636563456519752
1103	3	0.08276219370496563
1103	4	0.05236829999692105
1103	5	0.11226212930366586
1103	6	0.08949934369591972
1103	7	0.0844507464854587
1103	8	0.12355435084772637
1103	9	0.1323228896035431
1103	10-11	0.13321892561574344
1103	12-13	0.11843471375263448
1103	14-15	0.15942459806479548
1103	16-17	0.14833816167041647
1103	18-19	0.19685935299154522
1103	20-21	0.2032910327359687
1103	22-23	0.2010930852279671
1103	24-25	0.1318499371414248
1103	26-27	0.1367524249931833
1103	28-29	0.17264365916157232
1103	30-31	0.15613314220113494
1103	32-33	0.15584205881294366
1103	34-35	0.19763751165412202
1103	36-37	0.1729958369460718
1103	38-39	0.12527547988265297
1103	40-41	0.07296589441215673
1103	42-43	0.07715381593562398
1103	44-45	0.13194239409735786
1103	46-47	0.08736581179272918
1103	48-49	0.0793404476888
1103	50-51	0.10303975588849568
1103	52-53	0.09874795977752626
1103	54-55	0.13463448952314394
1103	56-57	0.0824771980560115
1103	58-59	0.024663866679908608
1103	60-61	0.028592166622694037
1103	62-63	-0.015850266115535305
1103	64-65	0.07884135176001905
1103	66-67	0.08421458693638328
1103	68-69	0.05708414103118287
1103	70-71	0.10146576970205956
1103	72-73	0.06612356174879608
1103	74-75	0.10186428697220862
1103	76-77	0.10951675291352814
1103	78-79	0.10232061555930727
1103	80-81	0.056008587886644534
1103	82-83	0.116692548254413
1103	84-85	0.1336224552826053
1103	86-87	0.1383374392592529
1103	88-89	0.14810828063720294
1103	90-91	0.15824554512738587
1103	92-93	0.18146329808748618
1103	94-95	0.1150322808919384
1103	96-97	0.15632837148253742
1103	98-99	0.1139517955981546
1103	100	0.07293349548312733
1104	1	0.11832904058514515
1104	2	0.14029477120109846
1104	3	0.1066266987724731
1104	4	0.0860071531846458
1104	5	0.09666020768670336
1104	6	0.08785122550644786
1104	7	0.11415646040169491
1104	8	0.09933708602896019
1104	9	0.15037829487699383
1104	10-11	0.1621940485758273
1104	12-13	0.16755716889012717
1104	14-15	0.2154751315589465
1104	16-17	0.23955954612488028
1104	18-19	0.23819008417190446
1104	20-21	0.2295096153455276
1104	22-23	0.21020919772754354
1104	24-25	0.2424636511385998
1104	26-27	0.23997050001576525
1104	28-29	0.2334461128211629
1104	30-31	0.24742424289072318
1104	32-33	0.20148464016980228
1104	34-35	0.19745695320288803
1104	36-37	0.235109483646184
1104	38-39	0.21789294843997453
1104	40-41	0.20381513052455347
1104	42-43	0.2674292148576427
1104	44-45	0.22741602904467584
1104	46-47	0.19320077477055264
1104	48-49	0.12889571528918253
1104	50-51	0.1695028599455881
1104	52-53	0.21977204508732484
1104	54-55	0.20964981829787632
1104	56-57	0.2313846984672736
1104	58-59	0.22178619162178137
1104	60-61	0.2634001852652972
1104	62-63	0.2029010259440227
1104	64-65	0.21571939277339425
1104	66-67	0.2576204163572484
1104	68-69	0.2775002655826455
1104	70-71	0.2810763440494668
1104	72-73	0.2749841653462468
1104	74-75	0.17735135494048393
1104	76-77	0.18358838337614003
1104	78-79	0.20223541660192978
1104	80-81	0.1899363345963465
1104	82-83	0.23962294374119608
1104	84-85	0.2553309472918528
1104	86-87	0.244210009417543
1104	88-89	0.2588359158009297
1104	90-91	0.2538009921865978
1104	92-93	0.3126542976995381
1104	94-95	0.3327360700242963
1104	96-97	0.2742367788646263
1104	98-99	0.27254083576614363
1104	100	0.30303006016616507
1105	1	0.08448017856134271
1105	2	0.0841495934411185
1105	3	0.1075861784535661
1105	4	0.08286967056860561
1105	5	0.06574643480489328
1105	6	0.09657557687167184
1105	7	0.09423909456339175
1105	8	0.10982071964127016
1105	9	0.1814470215722963
1105	10-11	0.15122924337217114
1105	12-13	0.15699654546154562
1105	14-15	0.1719635900238501
1105	16-17	0.21445137513781987
1105	18-19	0.24064749991779877
1105	20-21	0.20347164767594705
1105	22-23	0.2584315684057543
1105	24-25	0.27577178368979105
1105	26-27	0.26864978222743474
1105	28-29	0.2628964297399463
1105	30-31	0.2753223352968135
1105	32-33	0.2813213674413575
1105	34-35	0.26760800438842125
1105	36-37	0.2836756664007538
1105	38-39	0.2354980957759878
1105	40-41	0.2558879809573753
1105	42-43	0.25525130343667257
1105	44-45	0.23247653011021896
1105	46-47	0.2166252363363057
1105	48-49	0.243460001047751
1105	50-51	0.2801209305300105
1105	52-53	0.2605769133942957
1105	54-55	0.2404774942475072
1105	56-57	0.23850417526715262
1105	58-59	0.25205765452571427
1105	60-61	0.20620624266345544
1105	62-63	0.2769861593226821
1105	64-65	0.22344479536843664
1105	66-67	0.24430911919397147
1105	68-69	0.2798985662568114
1105	70-71	0.241612288227131
1105	72-73	0.24710471522120514
1105	74-75	0.21342710607090254
1105	76-77	0.24018717521462207
1105	78-79	0.23910335397449245
1105	80-81	0.27077367855395806
1105	82-83	0.2954647822155039
1105	84-85	0.2575324043713607
1105	86-87	0.23793722871246104
1105	88-89	0.2743592679517022
1105	90-91	0.2942797312776122
1105	92-93	0.33626166912019784
1105	94-95	0.2850144873994367
1105	96-97	0.3117145239958603
1105	98-99	0.32734598333832565
1105	100	0.37406949058321715
1106	1	4.6522489918743304E-4
1106	2	0.04607570239038239
1106	3	0.04297396361338457
1106	4	0.02605138814720931
1106	5	0.06785588305660895
1106	6	0.07313341768519166
1106	7	0.08936506991157955
1106	8	0.06490178571343819
1106	9	0.0448415350752569
1106	10-11	0.10354975717291381
1106	12-13	0.08045238668552201
1106	14-15	0.09579850416906766
1106	16-17	0.11860950729825248
1106	18-19	0.13219562246056427
1106	20-21	0.15572978748222255
1106	22-23	0.11346273528235429
1106	24-25	0.11599777875620276
1106	26-27	0.1188870046128514
1106	28-29	0.13165027781298733
1106	30-31	0.14150717962339598
1106	32-33	0.10442348775475097
1106	34-35	0.0610084333605343
1106	36-37	0.13279176653717428
1106	38-39	0.13880431989580444
1106	40-41	0.1766911165584375
1106	42-43	0.11791262069494479
1106	44-45	0.14021935609482483
1106	46-47	0.17373124395800943
1106	48-49	0.19588152047825957
1106	50-51	0.14299914832390925
1106	52-53	0.15953949302173953
1106	54-55	0.2004523032019918
1106	56-57	0.11582579570328733
1106	58-59	0.1494125947472824
1106	60-61	0.16663094920387067
1106	62-63	0.1334293929515198
1106	64-65	0.14305276475286632
1106	66-67	0.13445241841610311
1106	68-69	0.09694195843631803
1106	70-71	0.1019194902102214
1106	72-73	0.10913665875463607
1106	74-75	0.1132154369025713
1106	76-77	0.09395557051283987
1106	78-79	0.1356100078712572
1106	80-81	0.04836773250143267
1106	82-83	0.05259533702212593
1106	84-85	0.04918273978248067
1106	86-87	0.041697776959750854
1106	88-89	0.03989944686446023
1106	90-91	0.010836606300244966
1106	92-93	0.08011863549054965
1106	94-95	0.05214218507469326
1106	96-97	0.01712456258725581
1106	98-99	0.019104567810821038
1106	100	0.10919409772765931
1107	1	-0.037430482906934515
1107	2	-0.04358219319912138
1107	3	-0.013462414049193683
1107	4	4.063698556677764E-5
1107	5	0.03326150620660684
1107	6	0.041134904080820434
1107	7	0.02875312063789437
1107	8	0.015624295920815712
1107	9	0.028512526208899658
1107	10-11	0.057482883544892616
1107	12-13	0.006047832235438477
1107	14-15	0.029775482196498615
1107	16-17	0.04688574879992302
1107	18-19	0.06485653656510237
1107	20-21	0.05289601140634659
1107	22-23	0.06411165046720413
1107	24-25	0.08371586303034206
1107	26-27	0.0372890829448167
1107	28-29	0.08731631116810945
1107	30-31	0.07239328660977407
1107	32-33	0.11359037131623495
1107	34-35	0.11123214677459003
1107	36-37	0.07650998638389694
1107	38-39	0.08906949912233841
1107	40-41	0.052272202818834046
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2216	50-51	-0.2646608975839584
2216	52-53	-0.2643157762744863
2216	54-55	-0.25443012874451654
2216	56-57	-0.25369522367802233
2216	58-59	-0.27759284530940675
2216	60-61	-0.26160273160131453
2216	62-63	-0.3005418530204125
2216	64-65	-0.3642869633108461
2216	66-67	-0.34506378437980345
2216	68-69	-0.33358585350068637
2216	70-71	-0.3711353975224938
2216	72-73	-0.3373219118416202
2216	74-75	-0.3821580939987257
2216	76-77	-0.330023498107785
2216	78-79	-0.3839346813901443
2216	80-81	-0.3468016614601481
2216	82-83	-0.3313661449808478
2216	84-85	-0.4261666232877772
2216	86-87	-0.46985089866284113
2216	88-89	-0.6039872644770483
2216	90-91	-0.535692893621615
2216	92-93	-0.5087713446579514
2216	94-95	-0.4266063242276914
2216	96-97	-0.5114955694546452
2216	98-99	-0.5623098377493392
2216	100	-0.5475919524864494
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	23.0
3	14.0
4	20.0
5	38.0
6	93.0
7	202.0
8	506.0
9	1034.0
10	1783.0
11	2865.0
12	4671.0
13	7255.0
14	8309.0
15	11077.0
16	13103.0
17	18394.0
18	21639.0
19	11469.0
20	7518.0
21	7379.0
22	7495.0
23	8226.0
24	9689.0
25	11566.0
26	14903.0
27	19650.0
28	24963.0
29	32011.0
30	41352.0
31	52725.0
32	67751.0
33	87992.0
34	122932.0
35	188923.0
36	347940.0
37	783209.0
38	1448879.0
39	710024.0
40	2166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.48439253574837	19.97909162276387	13.620631551102411	23.915884290385343
2	15.531721778099886	20.68173960330722	39.94565390147086	23.84088471712203
3	17.86018143961347	25.97220352146619	30.429992174953213	25.737622863967125
4	12.161121214841296	17.213544614039673	39.36011353036908	31.265220640749945
5	12.570125782481774	37.92221818948157	36.00906488549619	13.49859114254047
6	30.292145015621575	38.674962723600466	17.91138010122009	13.121512159557868
7	26.545079891935874	32.828380394303316	23.205687708730306	17.420852005030504
8	24.98168805519707	35.82567662727515	20.845444746709767	18.347190570818018
9	25.22106021091822	16.13590751521786	21.491403945765	37.151628328098916
10-11	23.73522215865484	26.582509738676265	29.34475578640367	20.337512316265226
12-13	24.82849113173657	24.07757425505904	29.33180935209333	21.762125261111063
14-15	21.92966836976743	25.73719253510618	26.948209622133106	25.38492947299329
16-17	21.092153592125236	29.178757252979764	27.340793000991148	22.38829615390385
18-19	20.954480373289233	27.812072384548404	30.014573988296906	21.21887325386546
20-21	22.102230908309274	27.172652241122254	29.612376251208143	21.112740599360333
22-23	21.674593520574412	27.012859479918056	29.59974408364162	21.71280291586591
24-25	21.571231398333573	27.11222570930525	29.624059710222628	21.69248318213855
26-27	21.384579510261407	27.08261835576991	29.67872170670663	21.854080427262055
28-29	21.390663443401923	27.14396417170983	29.727824220683225	21.737548164205027
30-31	21.709733350683834	27.5416604112581	29.318180182841274	21.430426055216792
32-33	21.398435723993533	27.216687789710104	29.691998708225885	21.69287777807048
34-35	21.49226984419682	27.028080476356337	29.734354556869768	21.74529512257707
36-37	21.882143662062525	27.373147099313428	29.07368624914264	21.671022989481408
38-39	21.70237826931539	27.584377533667592	29.17732575440486	21.535918442612154
40-41	21.608317700407362	27.279465982013214	29.3133643284395	21.798851989139923
42-43	21.61997156926163	27.190735228260582	29.141275109834947	22.04801809264284
44-45	21.774699082001312	27.412917448414404	28.975522636780244	21.836860832804035
46-47	21.897961553133968	27.58880459184719	28.660664892916415	21.85256896210243
48-49	21.905827813535726	27.527642892754457	28.499790720886054	22.066738572823766
50-51	21.946659193109497	27.552485640720935	28.283133176642306	22.217721989527263
52-53	21.998381379720122	27.50049514755397	28.261058864507138	22.240064608218766
54-55	22.06705566239035	27.8610381805108	28.101074982413728	21.97083117468513
56-57	21.86669164356791	27.531155269491986	28.235472175634445	22.366680911305657
58-59	21.725867288747615	27.242006172026457	28.58556344864661	22.446563090579318
60-61	21.906474188421452	27.66585735652673	28.17848142391753	22.249187031134294
62-63	21.832763059943588	27.56668149670178	28.232654956792885	22.367900486561744
64-65	21.86555743857975	27.31370500133178	28.26565666322259	22.55508089686589
66-67	21.71789126657281	27.40547803935228	28.198726373168565	22.67790432090635
68-69	22.15736645445208	27.451088241054826	28.16974857925338	22.221796725239713
70-71	22.06608000218548	27.40377063399376	28.087318173525073	22.442831190295692
72-73	21.960446370782986	27.45394229513299	28.15855404765456	22.427057286429463
74-75	21.996826906389835	27.501189391783885	27.996593479486542	22.505390222339734
76-77	22.037956596780127	27.507336964740613	27.876368241479803	22.578338196999454
78-79	22.11918030883548	27.591109589081192	27.983215229665532	22.306494872417794
80-81	22.04861568451832	27.35740238275735	28.083720426519616	22.510261506204714
82-83	21.877369408074983	27.419794869705953	28.210177876422527	22.49265784579653
84-85	21.87739951431635	27.35306069484568	27.992703525157886	22.77683626568008
86-87	21.897537103937857	27.499977437742196	27.957783942355746	22.6447015159642
88-89	21.729101588154535	27.330981044811743	28.03226483527862	22.9076525317551
90-91	21.619800787643864	27.275208610005304	28.098599030098697	23.006391572252134
92-93	21.762022010304747	27.35139592876317	27.993652801510528	22.892929259421553
94-95	21.519623685747842	27.187570507090044	28.08404871986565	23.208757087296465
96-97	21.496782389108102	27.19325739594383	28.091268777527382	23.218691437420684
98-99	21.45707379081174	27.303708937943217	27.984415282214155	23.25480198903089
100	21.54923620440862	27.15303815709495	27.90593074568734	23.39179489280909
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24.0
1	28.0
2	100.5
3	163.5
4	110.5
5	95.5
6	220.0
7	504.5
8	902.5
9	1103.0
10	924.0
11	788.0
12	910.5
13	1110.5
14	1328.5
15	1547.5
16	1839.0
17	2178.0
18	2526.0
19	3020.0
20	3726.0
21	4683.5
22	6095.5
23	8535.5
24	11875.5
25	15865.0
26	21327.5
27	29148.0
28	39353.5
29	50794.0
30	62942.5
31	75467.0
32	88393.5
33	101974.0
34	113913.5
35	123441.5
36	131122.0
37	138343.0
38	144074.5
39	146407.5
40	148670.5
41	149960.0
42	149659.5
43	149934.0
44	147231.5
45	143569.5
46	141193.0
47	137207.5
48	133806.5
49	131603.0
50	128811.0
51	124278.5
52	115922.0
53	109573.0
54	105722.5
55	99512.0
56	91416.0
57	83146.5
58	75505.0
59	67517.0
60	59311.5
61	50532.5
62	42970.5
63	36385.5
64	29778.0
65	24372.0
66	19733.0
67	15502.5
68	12300.0
69	9739.5
70	7493.5
71	5533.5
72	4160.0
73	3289.0
74	2548.0
75	1762.5
76	1099.0
77	771.5
78	567.0
79	365.5
80	224.0
81	106.5
82	55.5
83	22.0
84	9.0
85	6.0
86	6.5
87	4.5
88	1.5
89	1.0
90	2.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.046563383277379224
2	0.24572002259629033
3	0.0032196786760681283
4	0.0022440184711989986
5	2.9269806146073893E-4
6	2.1952354609555423E-4
7	0.0
8	1.2195752560864123E-4
9	0.0
10-11	6.463748857257985E-4
12-13	0.0
14-15	2.6830655633901067E-4
16-17	1.8293628841296183E-4
18-19	4.3904709219110845E-4
20-21	1.2195752560864123E-5
22-23	2.6830655633901067E-4
24-25	0.0013293370291341893
26-27	2.1952354609555423E-4
28-29	2.6830655633901067E-4
30-31	6.0978762804320615E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	2.4391505121728245E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	1.7074053585209773E-4
70-71	0.0
72-73	3.048938140216031E-4
74-75	8.537026792604886E-5
76-77	0.0
78-79	0.0
80-81	0.0
82-83	8.537026792604886E-5
84-85	0.0
86-87	5.122216075562931E-4
88-89	0.0022562142237598625
90-91	0.0022562142237598625
92-93	6.463748857257985E-4
94-95	5.610046177997497E-4
96-97	1.7074053585209773E-4
98-99	6.0978762804320615E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4099788.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.33951513924281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.0168603626099	40.01055479540753
2	15.81749003535045	17.82299438622898
3	5.7813403117715785	9.771537300605088
4	2.7236432176466643	6.13794953178001
5	1.472445563595692	4.14784345609552
6	0.8583982181954898	2.9017043645714313
7	0.5344178880723662	2.1076191287014674
8	0.3755260439811774	1.6925564192046005
9	0.26553552442221623	1.3464128428373006
>10	1.0912189932462462	10.33052220408874
>50	0.04730190010588016	1.8234113279423876
>100	0.015172463149926124	1.3821023975110078
>500	4.7628381587746447E-4	0.17107637847855
>1k	1.2989552739186E-4	0.21235125077892855
>5k	4.329850913061994E-5	0.14136421576839633
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5795	0.14134877218041517	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4949	0.1207135588474331	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.4391505121728245E-5	0.0
4	0.0	0.0	0.0	4.878301024345649E-5	0.0
5	0.0	0.0	0.0	4.878301024345649E-5	0.0
6	0.0	0.0	0.0	4.878301024345649E-5	0.0
7	0.0	0.0	0.0	4.878301024345649E-5	0.0
8	0.0	0.0	0.0	9.756602048691298E-5	0.0
9	0.0	0.0	0.0	1.7074053585209773E-4	0.0
10-11	0.0	0.0	0.0	1.9513204097382596E-4	0.0
12-13	0.0	0.0	0.0	2.073277935346901E-4	0.0
14-15	0.0	0.0	0.0	3.4148107170419546E-4	0.0
16-17	0.0	0.0	0.0	4.390470921911084E-4	0.0
18-19	0.0	0.0	0.0	4.390470921911084E-4	0.0
20-21	0.0	0.0	0.0	4.6343859731283666E-4	0.0
22-23	0.0	0.0	0.0	5.244173601171573E-4	0.0
24-25	0.0	0.0	0.0	6.097876280432061E-4	0.0
26-27	0.0	0.0	0.0	7.195494010909832E-4	0.0
28-29	0.0	0.0	0.0	0.0015000775649862872	0.0
30-31	0.0	0.0	0.0	0.004414862427032812	0.0
32-33	0.0	0.0	0.0	0.012122578045498938	0.0
34-35	0.0	0.0	0.0	0.024330526358923923	0.0
36-37	0.0	0.0	0.0	0.04470962888812788	0.0
38-39	0.0	0.0	0.0	0.08077246921060309	0.0
40-41	0.0	0.0	0.0	0.12207948313424988	0.0
42-43	0.0	0.0	0.0	0.16642323944555182	0.0
44-45	0.0	0.0	0.0	0.21901132448799793	0.0
46-47	0.0	0.0	0.0	0.28245362930961304	0.0
48-49	0.0	0.0	0.0	0.35377439028554647	0.0
50-51	0.0	0.0	0.0	0.4306320229241122	0.0
52-53	0.0	0.0	0.0	0.5100385678478985	0.0
54-55	0.0	0.0	0.0	0.597055262369664	0.0
56-57	0.0	0.0	0.0	0.7041827528642944	0.0
58-59	0.0	0.0	0.0	0.823505995919789	0.0
60-61	0.0	0.0	0.0	0.951232112489719	0.0
62-63	0.0	0.0	0.0	1.083702376805825	0.0
64-65	0.0	0.0	0.0	1.2236608331943017	0.0
66-67	0.0	0.0	0.0	1.3840105878645432	0.0
68-69	0.0	2.4391505121728245E-5	0.0	1.5688616094295607	0.0
70-71	0.0	2.4391505121728245E-5	0.0	1.769079279221267	0.0
72-73	0.0	2.4391505121728245E-5	0.0	1.9819317486660286	0.0
74-75	0.0	2.4391505121728245E-5	0.0	2.1973453261485716	0.0
76-77	0.0	2.4391505121728245E-5	0.0	2.4309793579570456	0.0
78-79	0.0	2.4391505121728245E-5	0.0	2.7022006991581025	0.0
80-81	0.0	2.4391505121728245E-5	0.0	2.9972769323682105	0.0
82-83	0.0	2.4391505121728245E-5	0.0	3.3045001351289383	0.0
84-85	0.0	2.4391505121728245E-5	0.0	3.6169309242331558	0.0
86-87	0.0	2.4391505121728245E-5	0.0	3.951314067946928	0.0
88	0.0	2.4391505121728245E-5	0.0	4.222608583663351	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5030	0.0	53.13218	1
GTATCAA	9600	0.0	44.13987	1
ATCAACG	11560	0.0	36.063435	3
TCAACGC	11610	0.0	35.86545	4
CAACGCA	11910	0.0	34.88312	5
TATCAAC	12480	0.0	33.6733	2
AACGCAG	12370	0.0	33.661915	6
ACGCAGA	14360	0.0	28.800701	7
CGCAGAG	14570	0.0	28.22431	8
GTACATG	10520	0.0	27.06224	1
TACATGG	10725	0.0	24.906988	2
GCAGAGT	16565	0.0	24.683266	9
ATACGCG	50	0.0017598895	23.499327	86-87
ACATGGG	10945	0.0	23.31768	3
AGAGTAC	15135	0.0	21.472595	10-11
GAGTACT	11305	0.0	20.74446	12-13
CAGAGTA	16120	0.0	20.204262	10-11
AGTACTT	12095	0.0	19.719791	12-13
CATGGGG	8030	0.0	19.37257	4
GTACTTT	12780	0.0	18.221647	14-15
>>END_MODULE