FastQCFastQC Report
Wed 25 May 2016
SRR1033018_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033018_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2837286
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC28050.034.8579374
ATCAACG28300.034.5500073
CAACGCA28850.033.8913425
GTATCAA31200.033.603961
GGTATCA19650.031.3436051
AACGCAG31400.031.1329776
GTACATG70850.027.3399411
TATCAAC38650.025.9060572
TACATGG72050.025.8364262
ACGCAGA37750.025.3984837
CGCAGAG39550.024.1390468
ACATGGG74250.023.7413833
GTGTAGG10200.019.3592851
CATGGGG52500.019.3404084
GAGTACA52350.018.5008091
GCAGAGT51050.018.1510759
CCTATAC10400.017.627983
GTATAGA12200.017.341751
ACCGTAT1902.0144982E-417.3266668
CTGTCGC7550.016.8208799
Produced by FastQC (version 0.11.5)