FastQCFastQC Report
Wed 25 May 2016
SRR1033018_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033018_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2837286
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46260.16304313347332627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36870.1299481264842529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35600.053.2216951
GTATCAA72750.044.119111
ATCAACG81650.038.6811523
TCAACGC81850.038.5285344
CAACGCA84100.037.664735
AACGCAG88100.035.9546436
TATCAAC89650.035.5242462
TAGCGCG753.2775945E-431.3319474
ACGCAGA103950.030.4271687
CGCAGAG105750.029.8203778
GCAGAGT119650.026.0418519
GTGGTAT19950.025.7511251
GTACATG69650.025.6466541
TACATGG70550.023.7060832
TGGTATC19400.022.82732
ACATGGG73050.021.9391823
GAGTACT85850.021.130912-13
AGAGTAC111400.020.71415910-11
CAGAGTA118550.020.3766410-11
ACACCGT4050.019.7271756
Produced by FastQC (version 0.11.5)