##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033018_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2837286
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73581972349633	34.0	31.0	34.0	31.0	34.0
2	32.7356583016305	34.0	31.0	34.0	31.0	34.0
3	32.834367420133184	34.0	31.0	34.0	31.0	34.0
4	36.31531928751631	37.0	37.0	37.0	35.0	37.0
5	36.29871398230563	37.0	37.0	37.0	35.0	37.0
6	36.36800484688537	37.0	37.0	37.0	35.0	37.0
7	36.32105082110157	37.0	37.0	37.0	35.0	37.0
8	36.33109915602446	37.0	37.0	37.0	35.0	37.0
9	38.141280082444986	39.0	39.0	39.0	37.0	39.0
10-11	38.10001476763358	39.0	39.0	39.0	36.0	39.0
12-13	38.076929854797854	39.0	39.0	39.0	35.0	39.0
14-15	39.5858781948665	41.0	40.0	41.0	37.0	41.0
16-17	39.583948181466376	41.0	40.0	41.0	37.0	41.0
18-19	39.60128693406304	41.0	40.0	41.0	37.0	41.0
20-21	39.59278056565323	41.0	40.0	41.0	37.0	41.0
22-23	39.52074958252358	41.0	39.5	41.0	37.0	41.0
24-25	39.46963718144734	41.0	39.0	41.0	36.0	41.0
26-27	39.35923378890955	41.0	39.0	41.0	36.0	41.0
28-29	39.30613392516651	41.0	39.0	41.0	36.0	41.0
30-31	39.30316277597676	41.0	39.0	41.0	36.0	41.0
32-33	39.257965887118885	41.0	39.0	41.0	35.5	41.0
34-35	39.17884132935488	41.0	39.0	41.0	35.0	41.0
36-37	39.08865021009514	41.0	39.0	41.0	35.0	41.0
38-39	38.99083684196799	41.0	39.0	41.0	35.0	41.0
40-41	38.88351932092852	41.0	39.0	41.0	35.0	41.0
42-43	38.7642153452278	41.0	39.0	41.0	35.0	41.0
44-45	38.59902491324456	41.0	39.0	41.0	35.0	41.0
46-47	38.398110377311276	41.0	38.0	41.0	35.0	41.0
48-49	38.192668275246135	40.5	38.0	41.0	34.0	41.0
50-51	38.05763236416773	40.0	38.0	41.0	34.0	41.0
52-53	37.92237705328261	40.0	38.0	41.0	34.0	41.0
54-55	37.77881961846638	40.0	38.0	41.0	33.0	41.0
56-57	37.633609371772884	40.0	37.0	41.0	33.0	41.0
58-59	37.459590608771904	40.0	37.0	41.0	33.0	41.0
60-61	37.27383157707753	40.0	36.5	41.0	33.0	41.0
62-63	37.04997011228336	39.5	36.0	41.0	33.0	41.0
64-65	36.79043388646756	39.0	35.0	41.0	32.0	41.0
66-67	36.51633127573322	39.0	35.0	41.0	32.0	41.0
68-69	36.217290925201056	38.0	35.0	40.5	31.5	41.0
70-71	35.89271807635889	37.0	35.0	40.0	31.0	41.0
72-73	35.549459589198975	37.0	35.0	39.5	31.0	41.0
74-75	35.202575454148786	36.5	35.0	39.0	31.0	41.0
76-77	34.847024409946684	36.0	35.0	39.0	31.0	40.5
78-79	34.4835480103169	35.5	35.0	37.5	30.5	39.5
80-81	34.146646478359955	35.0	34.0	37.0	30.0	39.0
82-83	33.83928038978094	35.0	34.0	37.0	30.0	39.0
84-85	33.604288746358314	35.0	34.0	36.0	30.0	38.0
86-87	33.3896355531307	35.0	34.0	36.0	30.0	37.0
88-89	33.1750193670994	35.0	34.0	36.0	30.0	37.0
90-91	32.99014868434131	35.0	34.0	35.0	30.0	36.5
92-93	32.81360902637239	35.0	34.0	35.0	29.5	36.0
94-95	32.65724392958623	35.0	34.0	35.0	29.0	36.0
96-97	32.511879486241426	35.0	34.0	35.0	29.0	36.0
98-99	32.38910952226882	35.0	34.0	35.0	29.0	36.0
100	32.28883905253119	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12447970737559189
1101	2	0.17598265844500816
1101	3	0.19462977891431166
1101	4	0.13225230880202332
1101	5	0.11135951635972674
1101	6	0.10546762991222636
1101	7	0.15268245102729594
1101	8	0.14723109602952178
1101	9	0.23235632866854417
1101	10-11	0.23833312685775354
1101	12-13	0.2755378059203224
1101	14-15	0.3469355935314056
1101	16-17	0.33955487463718725
1101	18-19	0.33850593178615185
1101	20-21	0.31063378102884087
1101	22-23	0.3414302333953785
1101	24-25	0.3739408141663034
1101	26-27	0.3648220943263141
1101	28-29	0.366774861148329
1101	30-31	0.33948719659495197
1101	32-33	0.3391103217463325
1101	34-35	0.3082256742017435
1101	36-37	0.2627645611089804
1101	38-39	0.2793672083495693
1101	40-41	0.28184925011449025
1101	42-43	0.24302937342733344
1101	44-45	0.22779849183027068
1101	46-47	0.26864521095497906
1101	48-49	0.1885446118675631
1101	50-51	0.19323130293649626
1101	52-53	0.1996781318546894
1101	54-55	0.15554600862918022
1101	56-57	0.2216059650618334
1101	58-59	0.22824143636238858
1101	60-61	0.196510430143654
1101	62-63	0.12260638318042538
1101	64-65	0.12671620197585298
1101	66-67	0.17271907151371124
1101	68-69	0.139946652113224
1101	70-71	0.12127424336136983
1101	72-73	0.1814849858116645
1101	74-75	0.1399050673963771
1101	76-77	0.0988016281877222
1101	78-79	0.14031877940630721
1101	80-81	0.13680802524418567
1101	82-83	0.20746940724166762
1101	84-85	0.22217392537518066
1101	86-87	0.25903456952654125
1101	88-89	0.2561994643332923
1101	90-91	0.2777461586660763
1101	92-93	0.30712109252358744
1101	94-95	0.29318365496382626
1101	96-97	0.31972436180474517
1101	98-99	0.22805348973468398
1101	100	0.2765446425402587
1102	1	0.11715009295367906
1102	2	0.15823566651662446
1102	3	0.11918023220710694
1102	4	0.09922899273225738
1102	5	0.11363618070382842
1102	6	0.10990977283352521
1102	7	0.08456666319860773
1102	8	0.1324295265380897
1102	9	0.1408452262052151
1102	10-11	0.15268639476536094
1102	12-13	0.1235113845807092
1102	14-15	0.21807961432742928
1102	16-17	0.2416291180041128
1102	18-19	0.2169265108138987
1102	20-21	0.22230937268754047
1102	22-23	0.26789624738634643
1102	24-25	0.2838204984473123
1102	26-27	0.2550959978906704
1102	28-29	0.24466076954568905
1102	30-31	0.21529265923170726
1102	32-33	0.2479498188751208
1102	34-35	0.22541146383183985
1102	36-37	0.230471419691618
1102	38-39	0.21601225786403688
1102	40-41	0.230228435310039
1102	42-43	0.25598262245623005
1102	44-45	0.22991459420892113
1102	46-47	0.16933177592002124
1102	48-49	0.19647978141906464
1102	50-51	0.0824855629912804
1102	52-53	0.14883951177866805
1102	54-55	0.13775671466095218
1102	56-57	0.13559766485854396
1102	58-59	0.17541697846623805
1102	60-61	0.15266640233777906
1102	62-63	0.11469522289478817
1102	64-65	0.09614747605970564
1102	66-67	0.08595233575132966
1102	68-69	0.11790596741183634
1102	70-71	0.13456480113877234
1102	72-73	0.05907938885164299
1102	74-75	0.10373421517390824
1102	76-77	0.036762578403006785
1102	78-79	0.10660576859220328
1102	80-81	0.08614993912708968
1102	82-83	0.07064613894748817
1102	84-85	0.08362184702666298
1102	86-87	0.0851653255604603
1102	88-89	0.10740396847156575
1102	90-91	0.06653060883638062
1102	92-93	0.09258719061844545
1102	94-95	0.11488652357805051
1102	96-97	0.16162509678125048
1102	98-99	0.09647460253986395
1102	100	0.058559801676317136
1103	1	0.08855194057858995
1103	2	0.09599074877741032
1103	3	0.06079549690975483
1103	4	0.08628507381361317
1103	5	0.0817209536289738
1103	6	0.07957300204039086
1103	7	0.12934380342873908
1103	8	0.10002673292549957
1103	9	0.1325152141167507
1103	10-11	0.1566599623762599
1103	12-13	0.1458489846425266
1103	14-15	0.21123859456342586
1103	16-17	0.22126718319601224
1103	18-19	0.19908537680548477
1103	20-21	0.23962358701526654
1103	22-23	0.25348619189875876
1103	24-25	0.2328298234580899
1103	26-27	0.2516342960944158
1103	28-29	0.275047135609789
1103	30-31	0.22536245830315949
1103	32-33	0.2214179986455065
1103	34-35	0.26547481929871
1103	36-37	0.22413051135371376
1103	38-39	0.23092338712532978
1103	40-41	0.21233811846533968
1103	42-43	0.2628509660181777
1103	44-45	0.24316876711996116
1103	46-47	0.22627807651149112
1103	48-49	0.1818776688461199
1103	50-51	0.18791558870383085
1103	52-53	0.23876044667461116
1103	54-55	0.18763024156454122
1103	56-57	0.25903637617062714
1103	58-59	0.23350039585605487
1103	60-61	0.29412538179014547
1103	62-63	0.22388180683438463
1103	64-65	0.22179669980950933
1103	66-67	0.2518913759800725
1103	68-69	0.26274478037531424
1103	70-71	0.2069854449245696
1103	72-73	0.2561628157274498
1103	74-75	0.2635482222532133
1103	76-77	0.3053453730851885
1103	78-79	0.2760079885081552
1103	80-81	0.2381543923218743
1103	82-83	0.21736455425244117
1103	84-85	0.2535952546129039
1103	86-87	0.2529225153624921
1103	88-89	0.26217895166829663
1103	90-91	0.2583597552931778
1103	92-93	0.2951737678766051
1103	94-95	0.31863561673728924
1103	96-97	0.33734389138864174
1103	98-99	0.26526126368723624
1103	100	0.21581289868274212
1104	1	0.0707620505106874
1104	2	0.11841312258496117
1104	3	0.10666426314771371
1104	4	0.064268803208968
1104	5	0.05243973163861426
1104	6	0.09965817339413263
1104	7	0.06904675756022982
1104	8	0.07556294100400152
1104	9	0.09253669644405704
1104	10-11	0.13539574195034731
1104	12-13	0.14832187929787466
1104	14-15	0.13690042338021868
1104	16-17	0.18091689095485464
1104	18-19	0.15776882111094181
1104	20-21	0.22483982107192446
1104	22-23	0.1302100539207487
1104	24-25	0.18895088504222457
1104	26-27	0.20823076708483512
1104	28-29	0.19023029477342845
1104	30-31	0.15343624682682844
1104	32-33	0.14992332860403224
1104	34-35	0.16250157019533873
1104	36-37	0.1644768191371071
1104	38-39	0.16061607251747745
1104	40-41	0.1512443108318422
1104	42-43	0.13849818083028254
1104	44-45	0.1379048441656181
1104	46-47	0.11776033113832796
1104	48-49	0.06345413318626214
1104	50-51	0.11990323091458777
1104	52-53	0.160081422910892
1104	54-55	0.09280780431716096
1104	56-57	0.11785165800676367
1104	58-59	0.09995811426988155
1104	60-61	0.15400076300671373
1104	62-63	0.08792243895887708
1104	64-65	0.0973518308177006
1104	66-67	0.13064646784454226
1104	68-69	0.1328408797858458
1104	70-71	0.08337627376705825
1104	72-73	0.07524682393101756
1104	74-75	0.12063811707131578
1104	76-77	0.11971324989353604
1104	78-79	0.03980693147174463
1104	80-81	0.0796777115231464
1104	82-83	0.08004209948382623
1104	84-85	0.04472511185510086
1104	86-87	0.08650982002347263
1104	88-89	0.08888112629967537
1104	90-91	0.10897250519460044
1104	92-93	0.1655733090539684
1104	94-95	0.17764077680476476
1104	96-97	0.18320021106311657
1104	98-99	0.20579050610294303
1104	100	0.1831042642978815
1105	1	0.0025545057231823876
1105	2	0.05534476602603888
1105	3	0.12092137187826069
1105	4	0.02710292150459992
1105	5	0.015551505414293842
1105	6	0.06739554595593944
1105	7	0.12401522304647017
1105	8	0.11117506215401818
1105	9	0.13427279222982946
1105	10-11	0.15168585972092075
1105	12-13	0.19471471251708294
1105	14-15	0.17357336291366465
1105	16-17	0.20168890006186047
1105	18-19	0.22080637450258678
1105	20-21	0.1982036208725546
1105	22-23	0.19030129961181785
1105	24-25	0.1958836410571081
1105	26-27	0.21479156723035686
1105	28-29	0.2508672139980135
1105	30-31	0.21069073429932672
1105	32-33	0.2245756584397114
1105	34-35	0.21386206984833223
1105	36-37	0.24200155957010594
1105	38-39	0.15183674287003157
1105	40-41	0.2042065446848511
1105	42-43	0.26327433939840006
1105	44-45	0.2585291017635427
1105	46-47	0.2125962990879131
1105	48-49	0.22612974631262972
1105	50-51	0.22579948779909387
1105	52-53	0.2156228273084153
1105	54-55	0.26847619339934425
1105	56-57	0.21356716312564572
1105	58-59	0.27673123912249764
1105	60-61	0.20724623350771054
1105	62-63	0.29765964951432267
1105	64-65	0.3019263695417962
1105	66-67	0.27481623953146084
1105	68-69	0.3014688542586299
1105	70-71	0.2934604011816475
1105	72-73	0.2968551481789419
1105	74-75	0.21485201353290506
1105	76-77	0.1379156343880652
1105	78-79	0.1729113820231447
1105	80-81	0.2569161471907009
1105	82-83	0.19151010036070915
1105	84-85	0.16476526820587623
1105	86-87	0.19726608474723406
1105	88-89	0.19825969649350839
1105	90-91	0.22993864745107118
1105	92-93	0.22097692264976843
1105	94-95	0.18634806973825135
1105	96-97	0.1858558710566527
1105	98-99	0.18176592651737877
1105	100	0.2397592794377914
1106	1	0.05242236350369467
1106	2	0.12079991563959425
1106	3	0.0697129458712169
1106	4	0.04308674379990407
1106	5	0.05438415571871502
1106	6	0.0816500548463921
1106	7	0.06899748643957793
1106	8	0.07676329975488727
1106	9	0.036662055504372404
1106	10-11	0.07636917743754879
1106	12-13	0.06889004910662777
1106	14-15	0.11383350558158867
1106	16-17	0.15458135795557837
1106	18-19	0.12133449356414872
1106	20-21	0.12335059549968719
1106	22-23	0.08469954560464288
1106	24-25	0.1212696815701122
1106	26-27	0.15733625822032593
1106	28-29	0.12132130019944753
1106	30-31	0.0389411710825911
1106	32-33	0.12242563982422894
1106	34-35	0.05946798062044678
1106	36-37	0.07817291724519748
1106	38-39	0.12106266881133365
1106	40-41	0.10542008591178842
1106	42-43	0.07750630556667204
1106	44-45	0.10810057666770945
1106	46-47	0.07732546232328019
1106	48-49	-0.028141058618835757
1106	50-51	-0.0223430299548113
1106	52-53	-0.0024142569401206515
1106	54-55	0.009189416525352101
1106	56-57	-0.02227648023038853
1106	58-59	-0.03277655907924526
1106	60-61	-0.03178159098177247
1106	62-63	-0.04489964897507548
1106	64-65	-0.036886563810178075
1106	66-67	-0.05130630614335985
1106	68-69	-0.016239904381890824
1106	70-71	-0.014862188459844106
1106	72-73	0.02229006874136985
1106	74-75	-0.004812166225725889
1106	76-77	-0.003943397994561337
1106	78-79	0.0023894427469741686
1106	80-81	0.013216027793284013
1106	82-83	-0.0640011886470262
1106	84-85	0.012431165109809683
1106	86-87	-0.0010254433413763309
1106	88-89	0.060096021221276885
1106	90-91	0.01688847008500005
1106	92-93	0.05019313832358563
1106	94-95	0.03320135600463914
1106	96-97	0.011599013893892618
1106	98-99	0.03239311475885387
1106	100	0.06240560932806716
1107	1	0.03264964503214429
1107	2	0.04898145218383121
1107	3	-0.009876514546384385
1107	4	0.043165954369762005
1107	5	0.04841299892755302
1107	6	0.04636438632678619
1107	7	0.04870945920522729
1107	8	0.015405032107651095
1107	9	0.0827934112724904
1107	10-11	0.07476912392640855
1107	12-13	0.08249710483214301
1107	14-15	0.14175589154476853
1107	16-17	0.09744611382860313
1107	18-19	0.1192736985716607
1107	20-21	0.052923817750460955
1107	22-23	0.09040468689870096
1107	24-25	0.12173086311018011
1107	26-27	0.17555226896480036
1107	28-29	0.1782307942023209
1107	30-31	0.13697877322022123
1107	32-33	0.10844629442038212
1107	34-35	0.13354394531717162
1107	36-37	0.11228428906665044
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2216	34-35	-0.42805338392197
2216	36-37	-0.41156334461793165
2216	38-39	-0.45639319819886737
2216	40-41	-0.42970718166522204
2216	42-43	-0.38645016380124275
2216	44-45	-0.4404763089017152
2216	46-47	-0.32827529814445455
2216	48-49	-0.3665172088364841
2216	50-51	-0.4458262849071488
2216	52-53	-0.457761387741499
2216	54-55	-0.34983313836529106
2216	56-57	-0.4120267057597786
2216	58-59	-0.4065027348208403
2216	60-61	-0.44481876865565795
2216	62-63	-0.45224875003246723
2216	64-65	-0.4797569215673789
2216	66-67	-0.4920919359743223
2216	68-69	-0.47774623587798715
2216	70-71	-0.43147069905059254
2216	72-73	-0.41847416550696437
2216	74-75	-0.5032729037130608
2216	76-77	-0.4468538863264513
2216	78-79	-0.41543147680279446
2216	80-81	-0.42073882393555806
2216	82-83	-0.4277322128715255
2216	84-85	-0.45607730869384255
2216	86-87	-0.4387341001581788
2216	88-89	-0.5425286114841938
2216	90-91	-0.5683665634863289
2216	92-93	-0.5738058809827251
2216	94-95	-0.45704294563112313
2216	96-97	-0.522127474910512
2216	98-99	-0.6496173773688128
2216	100	-0.6438968952100126
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	13.0
3	5.0
4	6.0
5	24.0
6	50.0
7	141.0
8	360.0
9	622.0
10	1165.0
11	1993.0
12	3052.0
13	4857.0
14	5773.0
15	8112.0
16	9601.0
17	13281.0
18	15700.0
19	8450.0
20	5450.0
21	5042.0
22	5214.0
23	5559.0
24	6402.0
25	8094.0
26	10020.0
27	13073.0
28	16738.0
29	21693.0
30	27909.0
31	35955.0
32	46051.0
33	59942.0
34	83503.0
35	126281.0
36	231186.0
37	527282.0
38	1005634.0
39	520991.0
40	2062.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.72670630270902	19.909336167400866	13.583178389341782	23.780779140548333
2	15.581358785611195	20.203010533865413	40.37432937805501	23.841301302468384
3	18.24788498282292	25.330262712219852	30.057299228009477	26.36455307694775
4	12.133931620828779	16.880885405340248	39.23041975406112	31.75476321976985
5	12.45633580624253	37.702918888131904	36.187911023202155	13.652834282423406
6	30.685448014879025	38.61070608747876	17.51890805393752	13.1849378437047
7	26.85661579410747	32.64979279494559	22.69538566080402	17.798205750142916
8	24.89229105663319	36.15016656821543	20.46547463130129	18.49206774385009
9	24.83732693848981	16.376213043027736	21.577028188205208	37.209431830277246
10-11	23.61832388498872	26.77334669829567	29.107809462583656	20.500519954131956
12-13	24.7158904671577	24.07207098614662	29.319180371665034	21.892858175030646
14-15	21.762246605547013	25.95233949746165	26.913137812465195	25.372276084526145
16-17	20.79040053459595	29.53610901697152	27.379873632626705	22.293616815805827
18-19	20.601238652848718	27.990103888377444	30.266953232607612	21.141704226166226
20-21	21.808626590450626	27.273409743221116	29.927266748446712	20.990696917881547
22-23	21.348579142189752	27.097742293902705	30.065599742006654	21.488078821900885
24-25	21.27721291275796	27.298541266632263	29.96562523790592	21.45862058270385
26-27	21.048227812231698	27.177892660727654	30.10148085281558	21.672398674225068
28-29	21.028971953229124	27.2298436688538	30.17888444860101	21.56229992931607
30-31	21.35962468319163	27.724781066957366	29.653598178257155	21.261996071593845
32-33	20.982904085101044	27.33434345356795	30.223583382147588	21.459169079183415
34-35	21.139620750252178	27.13723607701162	30.257118950997537	21.46602422173866
36-37	21.532478572833334	27.596354403468666	29.49825995687428	21.372907066823718
38-39	21.366316261385	27.723535801466614	29.613669541949594	21.296478395198793
40-41	21.181361967754732	27.496114682894863	29.740020783957338	21.58250256539307
42-43	21.230658453183782	27.378452507078947	29.596135179886694	21.794753859850577
44-45	21.359055801917748	27.570907550384415	29.46171094489593	21.608325702801903
46-47	21.52729756534942	27.90547022753434	28.990926540362867	21.57630566675337
48-49	21.510785306803758	27.8076302494708	28.83357546613207	21.848008977593373
50-51	21.626212514353504	27.777636797982296	28.65158817264104	21.944562515023158
52-53	21.6079732533132	27.74129925569717	28.688313409363737	21.96241408162589
54-55	21.726836843377793	28.21180170063927	28.423412373655673	21.637949082327264
56-57	21.549149433648918	27.8804815587854	28.545906193453884	22.024462814111796
58-59	21.301077861026346	27.48772947105086	29.028973462668194	22.1822192052546
60-61	21.597012074214582	27.904536236389283	28.58323059430738	21.915221095088757
62-63	21.473778110490095	27.87390837582112	28.586684599296653	22.065628914392136
64-65	21.4822721431678	27.596601118110758	28.77237261241905	22.14875412630239
66-67	21.372889444349283	27.70897963758324	28.537887967585924	22.380242950481552
68-69	21.821965536316252	27.835760807943817	28.479952517845895	21.862321137894032
70-71	21.809133798989595	27.637291411581348	28.47989240422009	22.073682385208965
72-73	21.612121637198527	27.82156884064931	28.51018768546635	22.05612183668582
74-75	21.639277470098566	27.835809677612065	28.39142975112965	22.13348310115972
76-77	21.61775372662467	27.862489012387186	28.287454983389054	22.232302277599086
78-79	21.81331032543071	27.9306703659765	28.3129018364733	21.943117472119482
80-81	21.661298860953742	27.738462037313123	28.435889085555704	22.16435001617743
82-83	21.54170164000352	27.81462742227989	28.574791953069145	22.068878984647448
84-85	21.51399259715094	27.722407963102768	28.411711050630778	22.351888389115516
86-87	21.603327002127752	27.928863988574932	28.28929861779056	22.178510391506762
88-89	21.395625393982236	27.778027435702363	28.37151425402829	22.454832916287113
90-91	21.32034782706771	27.655064500762983	28.493228129209125	22.531359542960182
92-93	21.446443280020215	27.90224240755968	28.2349045541425	22.41640975827761
94-95	21.184751146571738	27.601799269542383	28.508324485309345	22.705125098576534
96-97	21.17980041775978	27.61510906674059	28.470718310323296	22.73437220517634
98-99	21.187287902169736	27.695789085432658	28.385392848753288	22.73153016364432
100	21.262784224078928	27.672007686218446	28.194619788065072	22.87058830163755
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9.0
1	18.0
2	63.5
3	109.0
4	78.0
5	63.0
6	177.5
7	420.0
8	734.5
9	868.5
10	758.5
11	666.0
12	746.5
13	914.5
14	1140.5
15	1405.5
16	1647.5
17	1881.5
18	2148.5
19	2558.0
20	3169.0
21	4128.5
22	5446.5
23	7297.0
24	9905.0
25	13142.0
26	17429.5
27	23182.5
28	30057.0
29	37849.0
30	46070.5
31	54578.5
32	63534.0
33	72747.0
34	80823.0
35	87466.5
36	93715.5
37	99250.5
38	103851.5
39	106660.5
40	107179.0
41	106718.0
42	105282.5
43	103949.0
44	101499.5
45	98276.0
46	96695.5
47	94743.0
48	91764.0
49	88827.0
50	86201.0
51	82259.0
52	76529.5
53	71476.0
54	68050.0
55	64430.0
56	59182.0
57	54511.0
58	50204.5
59	44525.5
60	38997.5
61	33358.0
62	28149.0
63	23575.0
64	18973.5
65	15369.0
66	12366.0
67	9505.5
68	7463.5
69	5716.5
70	4148.0
71	3062.5
72	2227.0
73	1640.0
74	1232.5
75	856.0
76	564.5
77	387.0
78	290.0
79	178.0
80	102.0
81	48.5
82	24.5
83	16.0
84	10.5
85	7.0
86	3.5
87	2.0
88	1.0
89	0.5
90	0.5
91	1.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.046347107764250765
2	0.24287294266422207
3	0.0026433711652614506
4	0.002713861063001756
5	2.467146420910687E-4
6	1.0573484661045802E-4
7	0.0
8	3.5244948870152674E-4
9	0.0
10-11	5.815416563575192E-4
12-13	0.0
14-15	3.5244948870152674E-4
16-17	1.0573484661045802E-4
18-19	3.5244948870152674E-4
20-21	1.7622474435076337E-5
22-23	1.2335732104553436E-4
24-25	0.0010573484661045803
26-27	7.048989774030535E-5
28-29	2.643371165261451E-4
30-31	1.0573484661045802E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	5.286742330522901E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	3.3482701426645043E-4
70-71	0.0
72-73	4.405618608769084E-4
74-75	1.9384721878583968E-4
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.9384721878583968E-4
84-85	0.0
86-87	5.991641307925955E-4
88-89	0.0024847688953457637
90-91	0.002520013844215916
92-93	8.106338240135116E-4
94-95	8.282562984485878E-4
96-97	3.3482701426645043E-4
98-99	1.5860226991568703E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2837286.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.427139926006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.77690169791295	50.23293090474559
2	14.322933328571647	18.742171260786243
3	4.409972682953713	8.6559569939243
4	1.8076225948585902	4.730703057888922
5	0.8639720932755829	2.8263611519452936
6	0.49328552755124255	1.9364556740741299
7	0.2991230272681615	1.3699534912115154
8	0.2123574447966587	1.1115152204032066
9	0.1431710198763876	0.8430543315721258
>10	0.6355895980778318	6.999685260517703
>50	0.025735461777166093	1.1413971862525618
>100	0.00890434426107186	0.9209928830040419
>500	2.1558940952409825E-4	0.08473818126882188
>1k	2.1558940948276947E-4	0.4040844024056008
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4626	0.16304313347332627	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3687	0.1299481264842529	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.5244948870152674E-5	0.0
5	0.0	0.0	0.0	3.5244948870152674E-5	0.0
6	0.0	0.0	0.0	3.5244948870152674E-5	0.0
7	0.0	0.0	0.0	3.5244948870152674E-5	0.0
8	0.0	0.0	0.0	3.5244948870152674E-5	0.0
9	0.0	0.0	0.0	2.1146969322091605E-4	0.0
10-11	0.0	0.0	0.0	2.1146969322091605E-4	0.0
12-13	0.0	0.0	0.0	2.1146969322091605E-4	0.0
14-15	0.0	0.0	0.0	2.1146969322091605E-4	0.0
16-17	0.0	0.0	0.0	2.1146969322091605E-4	0.0
18-19	0.0	0.0	0.0	2.1146969322091605E-4	0.0
20-21	0.0	0.0	0.0	2.2909216765599238E-4	0.0
22-23	0.0	0.0	0.0	3.3482701426645043E-4	0.0
24-25	0.0	0.0	0.0	4.053169120067558E-4	0.0
26-27	0.0	0.0	0.0	4.934292841821374E-4	0.0
28-29	0.0	0.0	0.0	0.001039725991669504	0.0
30-31	0.0	0.0	0.0	0.003401137565969733	0.0
32-33	0.0	0.0	0.0	0.00951613619494122	0.0
34-35	0.0	0.0	0.0	0.018151148668128628	0.0
36-37	0.0	0.0	0.0	0.03409948803187271	0.0
38-39	0.0	0.0	0.0	0.06220733475581947	0.0
40-41	0.0	0.0	0.0	0.09805144775676473	0.0
42-43	0.0	0.0	0.0	0.1358340329455684	0.0
44-45	0.0	0.0	0.0	0.1784275536551479	0.0
46-47	0.0	0.0	0.0	0.23143595675585754	0.0
48-49	0.0	0.0	0.0	0.2913699923095522	0.0
50-51	0.0	0.0	0.0	0.35812392546962135	0.0
52-53	0.0	0.0	0.0	0.42692206566415936	0.0
54-55	0.0	0.0	0.0	0.5004077840584277	0.0
56-57	0.0	0.0	0.0	0.5885906461315497	0.0
58-59	0.0	0.0	0.0	0.6910124675482133	0.0
60-61	0.0	0.0	0.0	0.800342298943427	0.0
62-63	0.0	0.0	0.0	0.916685875163801	0.0
64-65	0.0	0.0	0.0	1.0372940901974634	0.0
66-67	0.0	0.0	0.0	1.1751018402797602	0.0
68-69	0.0	0.0	0.0	1.3353782452667795	0.0
70-71	0.0	0.0	0.0	1.512501735813732	0.0
72-73	0.0	0.0	0.0	1.6988946479135343	0.0
74-75	0.0	0.0	0.0	1.8871731647778898	0.0
76-77	0.0	0.0	0.0	2.094237239390037	0.0
78-79	0.0	0.0	0.0	2.328845241544208	0.0
80-81	0.0	0.0	0.0	2.591931162385463	0.0
82-83	0.0	0.0	0.0	2.867458550177881	0.0
84-85	0.0	0.0	0.0	3.1411003332057468	0.0
86-87	0.0	0.0	0.0	3.4336334088280136	0.0
88	0.0	0.0	0.0	3.6725236722699086	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3560	0.0	53.221695	1
GTATCAA	7275	0.0	44.11911	1
ATCAACG	8165	0.0	38.681152	3
TCAACGC	8185	0.0	38.528534	4
CAACGCA	8410	0.0	37.66473	5
AACGCAG	8810	0.0	35.954643	6
TATCAAC	8965	0.0	35.524246	2
TAGCGCG	75	3.2775945E-4	31.331947	4
ACGCAGA	10395	0.0	30.427168	7
CGCAGAG	10575	0.0	29.820377	8
GCAGAGT	11965	0.0	26.041851	9
GTGGTAT	1995	0.0	25.751125	1
GTACATG	6965	0.0	25.646654	1
TACATGG	7055	0.0	23.706083	2
TGGTATC	1940	0.0	22.8273	2
ACATGGG	7305	0.0	21.939182	3
GAGTACT	8585	0.0	21.1309	12-13
AGAGTAC	11140	0.0	20.714159	10-11
CAGAGTA	11855	0.0	20.37664	10-11
ACACCGT	405	0.0	19.727175	6
>>END_MODULE