FastQCFastQC Report
Wed 25 May 2016
SRR1033017_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033017_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693495
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10160.14650430067988954No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG9590.13828506333859653No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA9340.13468013468013468No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA8440.12170239150967202No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA8170.11780906855853324No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT8120.11708808282684086No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA7830.11290636558302511No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA7820.11276216843668664No Hit
GTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGT7710.11117599982696343No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT7560.10901304263188631No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT7130.10281256533933195No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT250.005233095556.4056326
GGTATCA11650.038.341081
TATAAGG801.1560476E-535.2586143
CGTATCA1502.910383E-1134.43529594
GGGTAAG1606.002665E-1132.3250661
TCTAAGG600.004154879631.3409863
GTAAGAT2000.030.5574653
AAGACCG650.006138659528.9364135
GTACATA1853.255991E-1027.9568121
CGTTCCT856.786389E-427.6658028
GTATCAA19300.027.04161
TAGTACA700.00878898926.8850619
AAGAGCG700.00881380326.8695265
GTACATT700.00881690926.8675841
CTCGTTC350.00885101226.8481934-35
CGTACTC350.00887908826.83076184-85
GTCGCCG2650.026.5795694
CTATAAG1151.3741557E-424.527732
GTACAAA2900.024.31981
AACGCAG19400.023.744646
Produced by FastQC (version 0.11.5)