##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033017_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	693495
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.330227326801204	33.0	31.0	34.0	29.0	34.0
2	31.418132791152065	34.0	31.0	34.0	28.0	34.0
3	31.47162272258632	34.0	31.0	34.0	28.0	34.0
4	34.701562376080574	37.0	35.0	37.0	32.0	37.0
5	34.91458049445202	37.0	35.0	37.0	32.0	37.0
6	34.93844079625664	37.0	36.0	37.0	33.0	37.0
7	34.90792002826264	37.0	36.0	37.0	32.0	37.0
8	34.84419930929567	37.0	35.0	37.0	32.0	37.0
9	36.40183707164435	39.0	38.0	39.0	33.0	39.0
10-11	36.47013316606464	39.0	38.0	39.0	33.0	39.0
12-13	36.428642600162945	39.0	38.0	39.0	32.5	39.0
14-15	37.79635830106922	41.0	39.0	41.0	33.0	41.0
16-17	37.77894000677726	41.0	39.0	41.0	32.5	41.0
18-19	37.824154463983156	41.0	39.0	41.0	32.5	41.0
20-21	37.84404862327774	41.0	39.0	41.0	32.0	41.0
22-23	37.81085227723344	40.0	39.0	41.0	32.0	41.0
24-25	37.850646363708464	40.0	38.0	41.0	32.0	41.0
26-27	37.725703862320564	40.0	38.0	41.0	32.0	41.0
28-29	37.70237204305727	40.0	38.0	41.0	31.5	41.0
30-31	37.76160174190153	40.5	38.0	41.0	32.0	41.0
32-33	37.692189561568576	41.0	38.0	41.0	31.5	41.0
34-35	37.61624813444942	41.0	38.0	41.0	31.0	41.0
36-37	37.50448813617979	40.5	38.0	41.0	31.0	41.0
38-39	37.377625649788385	40.0	38.0	41.0	31.0	41.0
40-41	37.28626738476845	40.0	38.0	41.0	30.0	41.0
42-43	37.14560739442966	40.0	38.0	41.0	30.0	41.0
44-45	36.990385655267886	40.0	38.0	41.0	30.0	41.0
46-47	36.83890078515346	40.0	37.5	41.0	30.0	41.0
48-49	36.67455136662846	40.0	37.0	41.0	30.0	41.0
50-51	36.525443586471425	40.0	37.0	41.0	30.0	41.0
52-53	36.37286353903056	40.0	36.0	41.0	30.0	41.0
54-55	36.17967901715225	40.0	36.0	41.0	30.0	41.0
56-57	35.98202726768037	39.5	35.0	41.0	29.5	41.0
58-59	35.70763163396997	39.0	35.0	41.0	29.0	41.0
60-61	35.42007296375604	39.0	35.0	41.0	29.0	41.0
62-63	35.08868989682694	38.0	35.0	40.0	28.0	41.0
64-65	34.74763336433572	37.0	35.0	40.0	28.0	41.0
66-67	34.31679103670538	37.0	34.5	39.5	27.5	41.0
68-69	33.92248826595721	36.0	34.0	39.0	27.0	41.0
70-71	33.53353376736675	36.0	34.0	39.0	26.0	40.5
72-73	33.101431877663146	35.0	34.0	37.5	26.0	39.5
74-75	32.73728866105739	35.0	34.0	37.0	26.0	39.0
76-77	32.40549535324696	35.0	33.5	36.5	25.5	39.0
78-79	32.06839775340846	35.0	33.0	36.0	25.0	37.5
80-81	31.77365878629262	35.0	33.0	35.5	24.0	37.0
82-83	31.622569016359165	35.0	33.0	35.0	24.0	37.0
84-85	31.483479332944	35.0	33.0	35.0	24.0	36.0
86-87	31.399741887108053	35.0	33.0	35.0	24.0	36.0
88-89	31.23717835024045	35.0	33.0	35.0	23.0	36.0
90-91	31.088322194103778	35.0	33.0	35.0	22.5	35.5
92-93	30.911031081694894	35.0	33.0	35.0	19.5	35.0
94-95	30.731306642441545	35.0	33.0	35.0	18.5	35.0
96-97	30.567700560205914	35.0	33.0	35.0	12.0	35.0
98-99	30.376580941463168	35.0	32.0	35.0	2.0	35.0
100	30.23665491459924	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0061846343696352335
1101	2	-0.048251215240302514
1101	3	0.008345096444820399
1101	4	-0.1819944243682059
1101	5	-0.18467743124985958
1101	6	0.1363231826805631
1101	7	-0.08759900271554955
1101	8	0.17811615194420227
1101	9	0.0870213496027219
1101	10-11	0.00784141330058219
1101	12-13	0.07055357118519368
1101	14-15	0.17573628832455768
1101	16-17	0.14002725156558427
1101	18-19	0.07707687013255082
1101	20-21	0.13811637143599853
1101	22-23	0.1247567416294757
1101	24-25	0.038080223655953205
1101	26-27	-0.32614581894995354
1101	28-29	-0.27634821414266497
1101	30-31	-0.03655524707383506
1101	32-33	-0.039675970816801964
1101	34-35	0.057091747832963335
1101	36-37	0.10665014782454563
1101	38-39	0.14309759716375225
1101	40-41	0.12856662077766146
1101	42-43	0.2958369242498904
1101	44-45	0.2389385865575946
1101	46-47	0.17012463870462824
1101	48-49	0.18135503668898423
1101	50-51	0.12219828401176613
1101	52-53	0.07770631345671575
1101	54-55	0.12774381631538745
1101	56-57	0.08777578834114763
1101	58-59	0.18777126324739868
1101	60-61	0.13154024960828536
1101	62-63	0.0719651062138098
1101	64-65	0.08367529296325671
1101	66-67	0.23778053101067798
1101	68-69	0.13053502058384225
1101	70-71	0.09067471296467033
1101	72-73	0.24102991671213658
1101	74-75	0.21695508069482372
1101	76-77	0.3235658349815438
1101	78-79	0.3515279011729362
1101	80-81	0.2808648254818067
1101	82-83	0.2899240787373145
1101	84-85	0.20212250471420035
1101	86-87	0.2545625284882469
1101	88-89	0.2864897644783895
1101	90-91	0.3450186996856921
1101	92-93	0.2360968533589478
1101	94-95	0.20195857607514967
1101	96-97	0.338876311385512
1101	98-99	0.39388310640381263
1101	100	0.3280590059406734
1102	1	-0.21412650534042044
1102	2	-0.36629757076725866
1102	3	-0.2895238615021114
1102	4	-0.4263122764191323
1102	5	-0.39524936811348255
1102	6	-0.1619457399382611
1102	7	-0.35102179186981886
1102	8	-0.23004842657883273
1102	9	-0.220405123768721
1102	10-11	-0.29007927509287157
1102	12-13	-0.20856562333390372
1102	14-15	-0.2424337155867704
1102	16-17	-0.33355300002103405
1102	18-19	-0.3583080170067561
1102	20-21	-0.4126564309663081
1102	22-23	-0.3749442311552684
1102	24-25	-0.35294580559067157
1102	26-27	-0.4065443957318138
1102	28-29	-0.310083404725205
1102	30-31	-0.32680216532712336
1102	32-33	-0.355074610684575
1102	34-35	-0.2840453126159517
1102	36-37	-0.2570754536996134
1102	38-39	-0.183030063484523
1102	40-41	-0.23773540932693038
1102	42-43	-0.28748126846494415
1102	44-45	-0.128178513509269
1102	46-47	-0.3264674604159552
1102	48-49	-0.37411514260117684
1102	50-51	-0.3382367564263049
1102	52-53	-0.4398536497011065
1102	54-55	-0.27870104029032916
1102	56-57	-0.12757679881204353
1102	58-59	-0.4163021939866667
1102	60-61	-0.3552703791059031
1102	62-63	-0.3839353189852375
1102	64-65	-0.45825728081571526
1102	66-67	-0.4812960174329888
1102	68-69	-0.4542291011971997
1102	70-71	-0.47950079203804563
1102	72-73	-0.5070566876256493
1102	74-75	-0.4009259897469022
1102	76-77	-0.3910202583603706
1102	78-79	-0.429797407632595
1102	80-81	-0.31093652960354845
1102	82-83	-0.43857687385993316
1102	84-85	-0.5348944149269634
1102	86-87	-0.5527497593853816
1102	88-89	-0.353989309925133
1102	90-91	-0.41598789910053213
1102	92-93	-0.2915473925751648
1102	94-95	-0.3157994768413097
1102	96-97	-0.37086604575644344
1102	98-99	-0.5014523213817412
1102	100	-0.6697430809412417
1103	1	0.23259068268237826
1103	2	0.23628993664364728
1103	3	0.2711516610769422
1103	4	0.18186936566191036
1103	5	0.25030320677337414
1103	6	0.2989945208082858
1103	7	0.19703434905776618
1103	8	0.2145719881971928
1103	9	0.1400731659405423
1103	10-11	0.3154878574581801
1103	12-13	0.36388751918445195
1103	14-15	0.4431958740397164
1103	16-17	0.32780461956607354
1103	18-19	0.3654473907545963
1103	20-21	0.4373726256618298
1103	22-23	0.5108714382918365
1103	24-25	0.5040615378117153
1103	26-27	0.580667752873758
1103	28-29	0.5234397261118033
1103	30-31	0.39498326174661713
1103	32-33	0.44522476063565364
1103	34-35	0.2808055593763896
1103	36-37	0.3652646908204247
1103	38-39	0.42005991814229304
1103	40-41	0.33202104329902227
1103	42-43	0.3731895580933937
1103	44-45	0.3194045033318318
1103	46-47	0.33812513043736914
1103	48-49	0.2580038780437093
1103	50-51	0.3256895864888705
1103	52-53	0.3292306849608053
1103	54-55	0.4130410072920938
1103	56-57	0.48375587316504465
1103	58-59	0.52459897238748
1103	60-61	0.3701996632169866
1103	62-63	0.4586575888494906
1103	64-65	0.6241725576998718
1103	66-67	0.5720020566115167
1103	68-69	0.5302922275418567
1103	70-71	0.4505431744558521
1103	72-73	0.48207730745527044
1103	74-75	0.4200898769330692
1103	76-77	0.43782915786555776
1103	78-79	0.41673780958771545
1103	80-81	0.33509680040343426
1103	82-83	0.32615248859655566
1103	84-85	0.3906067385331191
1103	86-87	0.39472172696387275
1103	88-89	0.3426794503841002
1103	90-91	0.39250778936545316
1103	92-93	0.5347937615893237
1103	94-95	0.40384867380703326
1103	96-97	0.26166505070569457
1103	98-99	0.24801673477679387
1103	100	0.25270096303698963
1104	1	0.3245825782548373
1104	2	0.4394763495487304
1104	3	0.46003353793836155
1104	4	0.4496254799653414
1104	5	0.30823325626352727
1104	6	0.536201814793074
1104	7	0.4925680566119297
1104	8	0.5546136174971394
1104	9	0.7533524697569263
1104	10-11	0.7037220444135954
1104	12-13	0.7198075156415271
1104	14-15	0.8099084879390333
1104	16-17	0.7430436005339587
1104	18-19	0.7137728078779659
1104	20-21	0.5752121092813667
1104	22-23	0.575045882773189
1104	24-25	0.39281000162411317
1104	26-27	0.42846616077447663
1104	28-29	0.4499850477977887
1104	30-31	0.4671857395756973
1104	32-33	0.5077788731897641
1104	34-35	0.6547036561645072
1104	36-37	0.7144498194173252
1104	38-39	0.7008080276627808
1104	40-41	0.7079911942052348
1104	42-43	0.781251359889211
1104	44-45	0.76711378467283
1104	46-47	0.7331716866092464
1104	48-49	0.8866345397945778
1104	50-51	0.8741473094990226
1104	52-53	0.8247970538008289
1104	54-55	0.7675953191354097
1104	56-57	0.7815753171481674
1104	58-59	0.7089713774760042
1104	60-61	0.6403644089571827
1104	62-63	0.6765520984319835
1104	64-65	0.6621039357846996
1104	66-67	0.7168731275695421
1104	68-69	0.7417901073261888
1104	70-71	0.6596473694935483
1104	72-73	0.6194407113148372
1104	74-75	0.693438267672768
1104	76-77	0.5485105055034225
1104	78-79	0.6207147363267893
1104	80-81	0.570566403264344
1104	82-83	0.4551950146979067
1104	84-85	0.5371335706098286
1104	86-87	0.5951850743581666
1104	88-89	0.5427580589026064
1104	90-91	0.6501090116725585
1104	92-93	0.6981693885199398
1104	94-95	0.807837779330665
1104	96-97	0.6178939454256351
1104	98-99	0.7096823496393405
1104	100	0.7723154491476407
1105	1	-0.10093904929751218
1105	2	-0.167688203333082
1105	3	-0.1718393622240484
1105	4	-0.24654119290011067
1105	5	-0.08092631279711071
1105	6	-0.1570762253998268
1105	7	-0.22072585246432652
1105	8	-0.4525397775760709
1105	9	-0.2930392189154176
1105	10-11	-0.2134126303362862
1105	12-13	-0.15948979036756583
1105	14-15	-0.15145759976134343
1105	16-17	-0.16758855131141814
1105	18-19	-0.058211174467899696
1105	20-21	-0.13376744713375643
1105	22-23	-0.26087544227856796
1105	24-25	-0.16746386058934348
1105	26-27	-0.1970281921346171
1105	28-29	-0.14891342854145506
1105	30-31	-0.10944051102869423
1105	32-33	-0.11761319980710283
1105	34-35	-0.0866552409480974
1105	36-37	-0.18938536735200273
1105	38-39	-0.17941599633889638
1105	40-41	-0.05095364902755506
1105	42-43	-0.13554858060247454
1105	44-45	-0.16526679578116443
1105	46-47	-0.15410965133597898
1105	48-49	-0.24569087149465219
1105	50-51	-0.2333767727712157
1105	52-53	0.020768465015670756
1105	54-55	-0.09642508725677601
1105	56-57	-0.09400891810802392
1105	58-59	-0.23029605040116508
1105	60-61	-0.2075608585202815
1105	62-63	-0.22708162790416964
1105	64-65	-0.14425849463396645
1105	66-67	-0.19493679296206778
1105	68-69	-0.0870677049994768
1105	70-71	-0.009886234106701863
1105	72-73	0.024586940595398232
1105	74-75	-0.09191338926181203
1105	76-77	-0.013976322083642856
1105	78-79	-0.018060508215185678
1105	80-81	0.08511494593056312
1105	82-83	0.05535184389665204
1105	84-85	-0.07122734730589642
1105	86-87	-0.1937339819391788
1105	88-89	-0.21093951489389795
1105	90-91	-0.045214626764852994
1105	92-93	-0.0854497086893673
1105	94-95	-0.09574945613628572
1105	96-97	-0.10937201953884568
1105	98-99	-0.011096589377036281
1105	100	0.04857659145929105
1106	1	-0.3247680710958143
1106	2	-0.29974819402363906
1106	3	-0.27081532334528546
1106	4	-0.2246639986719643
1106	5	-0.4033703575139995
1106	6	-0.36554860869647854
1106	7	-0.32147297482040216
1106	8	-0.2560394975038065
1106	9	-0.3378389475214476
1106	10-11	-0.30606526972155024
1106	12-13	-0.17173210415895568
1106	14-15	-0.3221261440956056
1106	16-17	-0.31758598726954546
1106	18-19	-0.17699041440699403
1106	20-21	-0.25626257545418696
1106	22-23	-0.3204933605921383
1106	24-25	-0.2535762208490624
1106	26-27	-0.14279772027201432
1106	28-29	-0.17346215717115143
1106	30-31	-0.09640827510527572
1106	32-33	-0.19420211683470257
1106	34-35	-0.16368990679426787
1106	36-37	-0.24148373977710946
1106	38-39	-0.2136706096419445
1106	40-41	-0.24551906365284992
1106	42-43	-0.28443657536394085
1106	44-45	-0.2649537854382231
1106	46-47	-0.2944021887907198
1106	48-49	-0.2282958197010032
1106	50-51	-0.2788500554548605
1106	52-53	-0.35565655056581846
1106	54-55	-0.3586516223289209
1106	56-57	-0.26529086015278835
1106	58-59	-0.14647752145116044
1106	60-61	-0.08253225959165889
1106	62-63	-0.2985325994788042
1106	64-65	-0.22164818166307043
1106	66-67	-0.2789860856809767
1106	68-69	-0.25924508936118684
1106	70-71	-0.36569142214958816
1106	72-73	-0.3020019764322015
1106	74-75	-0.16172830488510925
1106	76-77	-0.2689890239526278
1106	78-79	-0.3526995305657401
1106	80-81	-0.053045492086681634
1106	82-83	-0.1581825497666145
1106	84-85	-0.14047324286655183
1106	86-87	-0.2436065687813027
1106	88-89	-0.3473228746565873
1106	90-91	-0.3863110899649378
1106	92-93	-0.31227668714352674
1106	94-95	-0.27184883839943197
1106	96-97	-0.16650281723199356
1106	98-99	-0.22013602039642421
1106	100	-0.15874986235245458
1107	1	-0.10079901131921432
1107	2	0.0365030399987738
1107	3	-0.0017707062712197796
1107	4	0.04666379095817774
1107	5	-0.05071078501264026
1107	6	0.10258126037697934
1107	7	0.07524628184516757
1107	8	0.006066863211579232
1107	9	0.04095509367549255
1107	10-11	0.04755841167698094
1107	12-13	0.0576685029615831
1107	14-15	0.16893435720405137
1107	16-17	0.11894782402023196
1107	18-19	-0.024647805415547452
1107	20-21	-0.01264113101015596
1107	22-23	-0.07152402350713771
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2216	9	-0.25461985511137897
2216	10-11	-0.08226479941584586
2216	12-13	-0.26107017390429377
2216	14-15	-0.3091902357551817
2216	16-17	-0.2032362432692807
2216	18-19	-0.24132848415232644
2216	20-21	-0.14567527704355854
2216	22-23	-0.02901890219418135
2216	24-25	-0.12338492984324745
2216	26-27	-0.1500436801459344
2216	28-29	-0.16742198105668393
2216	30-31	-0.2998609360015294
2216	32-33	-0.43571118105978
2216	34-35	-0.551128825424712
2216	36-37	-0.6248208255495271
2216	38-39	-0.5537887697793593
2216	40-41	-0.44793563442385675
2216	42-43	-0.5458406546821664
2216	44-45	-0.3837589254458891
2216	46-47	-0.3517144417551421
2216	48-49	-0.36168293799456563
2216	50-51	-0.31459787487082025
2216	52-53	-0.2342026055015225
2216	54-55	-0.3018938964737856
2216	56-57	-0.4283743644305673
2216	58-59	-0.32697484471127325
2216	60-61	-0.20466664694343706
2216	62-63	-0.3250085588523959
2216	64-65	-0.4163901806290653
2216	66-67	-0.4353243722339002
2216	68-69	-0.4979168927000259
2216	70-71	-0.43931219231686924
2216	72-73	-0.3694604077458905
2216	74-75	-0.42912377860969997
2216	76-77	-0.2096583756979271
2216	78-79	-0.3179177557454409
2216	80-81	-0.2825863603176977
2216	82-83	-0.4638224657119778
2216	84-85	-0.2717389822638303
2216	86-87	-0.221219517197774
2216	88-89	-0.3281253962956505
2216	90-91	-0.27936657603087767
2216	92-93	-0.45574077213210273
2216	94-95	-0.36088879770631266
2216	96-97	-0.4903758346933458
2216	98-99	-0.3059713967243667
2216	100	-0.26070863555503365
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10923.0
3	1613.0
4	1142.0
5	1138.0
6	1729.0
7	3300.0
8	3997.0
9	2829.0
10	1949.0
11	1905.0
12	1860.0
13	1594.0
14	1607.0
15	1603.0
16	1672.0
17	1718.0
18	1654.0
19	1404.0
20	1356.0
21	1344.0
22	1528.0
23	2601.0
24	4218.0
25	5370.0
26	4967.0
27	3903.0
28	4375.0
29	5517.0
30	7057.0
31	8787.0
32	11463.0
33	15626.0
34	24093.0
35	39641.0
36	76080.0
37	172993.0
38	218113.0
39	40816.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.23835934253659	21.390357357218708	13.3185107807378	23.052772519506902
2	16.844051943255945	21.29315741945535	37.57288472024673	24.28990591704197
3	17.40044408983946	27.592147198901174	29.408327478022745	25.59908123323662
4	12.283106867849598	19.050518927280912	38.373344800914325	30.293029403955163
5	11.855510158134084	40.645814000141435	35.403013163648644	12.095662678075836
6	28.588974586936693	40.28464131903151	17.474800540359546	13.65158355367225
7	26.754918332089712	34.308027370332255	22.636667643285076	16.300386654292957
8	25.14986914867972	35.92500263434815	19.571835426136484	19.353292790835646
9	25.11586887583735	18.093875801267014	20.68492181827892	36.10533350461671
10-11	24.04473929982433	28.31181822512266	27.87956735939397	19.763875115659044
12-13	23.86840750971339	27.68483224693044	26.19103127555561	22.25572896780056
14-15	22.049936667977814	28.276105964757804	24.672784552038713	25.00117281522567
16-17	21.82242188187723	30.60233174641428	25.549276506334444	22.025969865374044
18-19	21.450733002165844	30.057527300388347	27.51105475377868	20.980684943667125
20-21	22.55950014036541	29.16143754388674	27.206858203510365	21.072204112237483
22-23	22.547865356591494	28.829372808726138	26.934164394234518	21.68859744044785
24-25	22.08266748971668	30.037599732316444	25.751201403031054	22.128531374935818
26-27	21.22836631700293	29.67403481324663	26.63923384565824	22.4583650240922
28-29	22.577005268117	28.851994915134398	27.422634000782065	21.148365815966535
30-31	22.644520765390055	29.257410026263386	26.527172501370895	21.57089670697567
32-33	21.4374253242443	28.774264209317224	27.362134846377177	22.426175620061297
34-35	22.437298576250946	28.787307338116424	26.930512998209405	21.844881087423225
36-37	23.05304601996151	28.440243877807177	25.93625475016375	22.570455352067565
38-39	21.90270307712955	28.983651080668043	26.876214912742746	22.23743092945966
40-41	21.67944847253331	28.83988154339945	27.57262105433302	21.90804892973422
42-43	21.822917794084752	29.030817296938473	26.93104169823437	22.215223210742398
44-45	21.03484775347904	28.77051942251593	27.3431879954659	22.851444828539126
46-47	22.414819486366138	28.674294711336213	26.528320865663048	22.3825649366346
48-49	21.98701769120981	28.324984883316525	26.898965715794805	22.789031709678863
50-51	21.80606282748201	28.425979505813327	26.426634650212055	23.34132301649261
52-53	21.558099899993216	28.663055070112982	26.82598964143311	22.952855388460687
54-55	21.470010411022553	29.3100226474011	26.831362357696186	22.38860458388016
56-57	21.82442996484155	28.41737908284999	26.60909617600438	23.149094776304086
58-59	22.318355202544222	27.266037200866062	26.784306114601065	23.631301481988654
60-61	22.351402360016507	27.76042282907628	27.070208903882282	22.81796590702493
62-63	21.38664180849007	27.66616931073394	27.24142061016049	23.705768270615497
64-65	21.776797225530945	28.210252462543295	26.334224824629686	23.678725487296067
66-67	21.723994877437274	27.2402734438412	26.70983568117458	24.325895997546944
68-69	21.97982013441219	28.245318480413555	26.366145155064626	23.40871623010963
70-71	22.715284150261727	27.657614372861012	26.715160067178008	22.911941409699256
72-73	22.17314239090679	27.08074077120021	26.545022749619996	24.201094088273
74-75	21.840712356951837	27.233672327633414	27.55910397003518	23.36651134537957
76-77	21.885088084419074	27.653912382315447	26.97025811789844	23.490741415367037
78-79	22.011352758587606	28.00397613453646	26.683310838934087	23.30136026794184
80-81	22.123434206748907	27.557312036284998	26.981486744405125	23.33776701256097
82-83	22.01711369745026	27.570264698201925	27.035732165296793	23.376889439051027
84-85	22.233947098129278	27.330036583536256	27.02868403134413	23.40733228699034
86-87	22.958661484055103	26.531287889552384	27.125547538968764	23.384503087423752
88-89	22.302492969297163	27.232676413118977	27.325433364661045	23.139397252922816
90-91	22.39712071433981	26.715760699049014	27.29789282739072	23.589225759220458
92-93	22.498790065772816	27.009987457075287	26.98106443918557	23.51015803796633
94-95	22.527685736418228	26.365245439084166	27.35676769658423	23.750301127913367
96-97	22.4474884395586	27.014106226950346	26.855278637112136	23.683126696378917
98-99	21.41268236105361	26.991167038613735	27.075197742639563	24.5209528576931
100	21.64086405913897	27.333884442982516	27.115604626435662	23.909646871442856
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	714.0
1	526.0
2	316.0
3	265.0
4	206.0
5	178.5
6	166.0
7	158.5
8	168.0
9	170.5
10	161.5
11	156.0
12	166.5
13	160.5
14	158.5
15	175.0
16	189.0
17	218.5
18	227.0
19	246.0
20	289.5
21	380.0
22	527.0
23	699.5
24	963.0
25	1283.5
26	1540.5
27	1879.5
28	2458.0
29	3259.5
30	4417.0
31	5513.5
32	6717.5
33	7702.0
34	8563.0
35	11585.5
36	15171.5
37	18436.5
38	22656.0
39	24952.5
40	26474.0
41	28608.0
42	31724.5
43	36611.5
44	38713.0
45	38046.0
46	38773.0
47	37750.0
48	34309.5
49	33478.5
50	34317.0
51	31659.5
52	25486.0
53	19531.0
54	15690.0
55	14949.5
56	12705.5
57	9055.0
58	7701.0
59	6862.5
60	5630.5
61	4171.5
62	3121.0
63	2588.5
64	2269.5
65	1966.5
66	1642.0
67	1249.0
68	910.5
69	748.5
70	553.0
71	408.0
72	373.0
73	331.0
74	251.0
75	219.0
76	249.5
77	210.0
78	156.5
79	197.0
80	165.0
81	111.5
82	91.5
83	33.0
84	10.0
85	4.5
86	4.5
87	4.0
88	1.5
89	2.0
90	2.0
91	2.0
92	1.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05868823855975891
2	0.03994260953575729
3	0.056525281364681795
4	0.07599189612037578
5	0.08680668209576133
6	0.08983482216886927
7	0.09055580790056166
8	0.10439873394905515
9	0.09891924238819313
10-11	0.10468712824173208
12-13	0.1103829155221018
14-15	0.10346145249785507
16-17	0.077578064730099
18-19	0.10000072098573169
20-21	0.09524221515656205
22-23	0.05839984426708195
24-25	0.02004340334104788
26-27	0.1814721086669695
28-29	0.06604229302302107
30-31	0.07426153036431409
32-33	0.0719543760228985
34-35	0.021557473377601857
36-37	0.052055169828189106
38-39	0.07844324760812983
40-41	0.08406693631533031
42-43	0.07786645902277595
44-45	0.07548720610819112
46-47	0.08212027483976092
48-49	0.07959682477883763
50-51	0.0746941218033295
52-53	0.07815485331545288
54-55	0.06914253166929826
56-57	0.070584503132683
58-59	0.06827734879126743
60-61	0.06474451870597481
62-63	0.07166598173022157
64-65	0.06654698303520573
66-67	0.0695751231083137
68-69	0.06885413737662131
70-71	0.059265027145112795
72-73	0.05861613998658967
74-75	0.05991391430363593
76-77	0.05501121132812782
78-79	0.05183887410868139
80-81	0.04556629824295777
82-83	0.05277615555988147
84-85	0.04138458099914203
86-87	0.040303102401603466
88-89	0.041168285279634315
90-91	0.03857273664554178
92-93	0.03972631381624958
94-95	0.038644835218711024
96-97	0.04188927101132669
98-99	0.042682355316188296
100	0.05018060692578894
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	693495.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.635768371795404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.04079059515837	17.74780230423339
2	9.08614843780913	4.476884966112328
3	4.153310282876385	3.06959970315416
4	2.467656908836082	2.4317049610858543
5	1.741250552906181	2.144852264932868
6	1.3259075384279875	1.959885059947759
7	1.0477117959310482	1.8067829587468558
8	0.831954017854103	1.6396661183870576
9	0.7139896369872105	1.583071498501128
>10	5.464370826657064	26.971998031539
>50	0.6532214878689923	11.256041022562883
>100	0.4526351223013426	21.522897777300535
>500	0.02046799648649482	3.2424382656094424
>1k	5.847998996141377E-4	0.14637506788674992
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1016	0.14650430067988954	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG	959	0.13828506333859653	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA	934	0.13468013468013468	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA	844	0.12170239150967202	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA	817	0.11780906855853324	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT	812	0.11708808282684086	No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA	783	0.11290636558302511	No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA	782	0.11276216843668664	No Hit
GTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGT	771	0.11117599982696343	No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT	756	0.10901304263188631	No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT	713	0.10281256533933195	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.4419714633847396E-4	0.0
5	0.0	0.0	0.0	1.4419714633847396E-4	0.0
6	0.0	0.0	0.0	1.4419714633847396E-4	0.0
7	0.0	0.0	0.0	1.4419714633847396E-4	0.0
8	0.0	0.0	0.0	1.4419714633847396E-4	0.0
9	0.0	0.0	0.0	1.4419714633847396E-4	0.0
10-11	0.0	0.0	0.0	1.4419714633847396E-4	0.0
12-13	0.0	0.0	0.0	1.4419714633847396E-4	0.0
14-15	0.0	0.0	0.0	2.883942926769479E-4	0.0
16-17	0.0	0.0	0.0	2.883942926769479E-4	0.0
18-19	0.0	0.0	0.0	5.046900121846589E-4	0.0
20-21	0.0	0.0	0.0	7.209857316923698E-4	0.0
22-23	0.0	0.0	0.0	0.0014419714633847397	0.0
24-25	0.0	0.0	0.0	0.003749125804800323	0.0
26-27	0.0	0.0	0.0	0.008651828780308438	0.0
28-29	0.0	0.0	0.0	0.018024643292309246	0.0
30-31	0.0	0.0	0.0	0.047152466852680985	0.0
32-33	0.0	0.0	0.0	0.1099503240830864	0.0
34-35	0.0	0.0	0.0	0.19819897764223246	0.0
36-37	0.0	0.0	0.0	0.33374429520039794	0.0
38-39	0.0	0.0	0.0	0.5404509044766004	0.0
40-41	0.0	0.0	0.0	0.761793524106158	0.0
42-43	0.0	0.0	0.0	0.9509080815290665	0.0
44-45	0.0	0.0	0.0	1.1956106388654568	0.0
46-47	0.0	0.0	0.0	1.4809767914692968	0.0
48-49	0.0	0.0	0.0	1.7617286353903057	0.0
50-51	0.0	0.0	0.0	2.048392562311192	0.0
52-53	0.0	0.0	0.0	2.3254673790005693	0.0
54-55	0.0	0.0	0.0	2.6323910049820114	0.0
56-57	0.0	0.0	0.0	3.000021629571951	0.0
58-59	0.0	0.0	0.0	3.396275387710077	0.0
60-61	0.0	0.0	0.0	3.822522152286606	0.0
62-63	0.0	0.0	0.0	4.237088948009719	0.0
64-65	0.0	0.0	0.0	4.678476412951788	0.0
66-67	0.0	0.0	0.0	5.165358077563645	0.0
68-69	0.0	0.0	0.0	5.729745708332432	0.0
70-71	0.0	0.0	0.0	6.327875471344422	0.0
72-73	0.0	0.0	0.0	6.938982977526875	0.0
74-75	0.0	0.0	0.0	7.587149150318315	0.0
76-77	0.0	0.0	0.0	8.231854591597632	0.0
78-79	0.0	0.0	0.0	8.967981023655542	0.0
80-81	0.0	0.0	0.0	9.769212467285271	0.0
82-83	0.0	0.0	0.0	10.609953929011745	0.0
84-85	0.0	0.0	0.0	11.460717092408743	0.0
86-87	0.0	0.0	0.0	12.34241054369534	0.0
88	0.0	0.0	0.0	13.044362252071032	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	25	0.0052330955	56.405632	6
GGTATCA	1165	0.0	38.34108	1
TATAAGG	80	1.1560476E-5	35.258614	3
CGTATCA	150	2.910383E-11	34.435295	94
GGGTAAG	160	6.002665E-11	32.325066	1
TCTAAGG	60	0.0041548796	31.340986	3
GTAAGAT	200	0.0	30.557465	3
AAGACCG	65	0.0061386595	28.936413	5
GTACATA	185	3.255991E-10	27.956812	1
CGTTCCT	85	6.786389E-4	27.665802	8
GTATCAA	1930	0.0	27.0416	1
TAGTACA	70	0.008788989	26.885061	9
AAGAGCG	70	0.008813803	26.869526	5
GTACATT	70	0.008816909	26.867584	1
CTCGTTC	35	0.008851012	26.84819	34-35
CGTACTC	35	0.008879088	26.830761	84-85
GTCGCCG	265	0.0	26.57956	94
CTATAAG	115	1.3741557E-4	24.52773	2
GTACAAA	290	0.0	24.3198	1
AACGCAG	1940	0.0	23.74464	6
>>END_MODULE