FastQCFastQC Report
Wed 25 May 2016
SRR1033017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033017_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693495
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29200.42105566730834393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28540.4115386556500047No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT18460.2661879321408229No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13330.1922147960691858No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG10650.15356996085047475No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA10570.15241638367976698No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA9610.13857345763127346No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT9570.13799666904591956TruSeq Adapter, Index 3 (95% over 21bp)
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT8840.12747027736321098No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA8600.1240095458510876No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA8250.11896264572924102No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA7640.1101666198025941No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT7480.10785946546117851No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT7210.10396614251003973No Hit
GTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGT7170.10338935392468582No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA7020.10122639672960872No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.058.9397351
GTATAAC407.903749E-658.9079781
TATAACG250.00519020156.5230522
GGACCGT451.6138387E-552.219186
AACACGT450.00101396841.7753454
GTATCAA34050.037.784151
CAACGAG500.001679262537.7011071
GCTCTAG500.001679262537.7011071
AACGAGA550.002687394434.2563972
GAGTTAC1001.4107372E-632.8980837
CTTCGTA600.00410553231.417591
GTACATT300.004162462431.33150914-15
TATAAGG600.004161040331.3315093
AAATACG2000.030.548225
ATCAACG43100.029.550483
CAACGCA43500.029.3867935
TCAACGC43500.029.3867934
ACTACGC804.780581E-429.373298
TAACTCG650.00615425928.921395
ATGGGAC1154.195139E-628.6070295
Produced by FastQC (version 0.11.5)