##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033017_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 693495 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.59423932400378 34.0 31.0 34.0 31.0 34.0 2 32.58349663660156 34.0 31.0 34.0 31.0 34.0 3 32.65202921434185 34.0 31.0 34.0 31.0 34.0 4 36.192901174485755 37.0 35.0 37.0 35.0 37.0 5 36.1979365388359 37.0 35.0 37.0 35.0 37.0 6 36.29126669983201 37.0 37.0 37.0 35.0 37.0 7 36.240021917966246 37.0 37.0 37.0 35.0 37.0 8 36.25291602679183 37.0 37.0 37.0 35.0 37.0 9 38.0361444567012 39.0 39.0 39.0 35.0 39.0 10-11 37.99023424826423 39.0 38.0 39.0 35.0 39.0 12-13 37.97083468518158 39.0 38.0 39.0 35.0 39.0 14-15 39.3908002220636 41.0 39.0 41.0 36.0 41.0 16-17 39.36328308062784 41.0 39.0 41.0 36.0 41.0 18-19 39.42711483139749 41.0 39.0 41.0 36.0 41.0 20-21 39.40700581835486 41.0 39.0 41.0 36.0 41.0 22-23 39.31544711930151 41.0 39.0 41.0 36.0 41.0 24-25 39.24809767914693 41.0 39.0 41.0 36.0 41.0 26-27 39.0998702225683 41.0 39.0 41.0 35.5 41.0 28-29 39.03221941037787 40.0 39.0 41.0 35.0 41.0 30-31 38.933091803113214 40.0 38.5 41.0 35.0 41.0 32-33 38.81368358820178 41.0 38.0 41.0 35.0 41.0 34-35 38.696850013338235 41.0 38.0 41.0 35.0 41.0 36-37 38.56654265712081 41.0 38.0 41.0 35.0 41.0 38-39 38.42314796790171 40.0 38.0 41.0 35.0 41.0 40-41 38.29898196814685 40.0 38.0 41.0 34.0 41.0 42-43 38.11505634503493 40.0 38.0 41.0 34.0 41.0 44-45 37.814266144673 40.0 38.0 41.0 33.0 41.0 46-47 37.41443846026287 40.0 37.5 41.0 33.0 41.0 48-49 37.00692362598144 40.0 37.0 41.0 31.5 41.0 50-51 36.762841116374304 40.0 37.0 41.0 31.0 41.0 52-53 36.603033186973235 40.0 36.5 41.0 31.0 41.0 54-55 36.39819969862796 40.0 36.0 41.0 31.0 41.0 56-57 36.207257442375216 39.5 35.5 41.0 30.5 41.0 58-59 35.94435215827079 39.0 35.0 41.0 30.0 41.0 60-61 35.66434076669623 39.0 35.0 41.0 29.5 41.0 62-63 35.338987303441264 38.5 35.0 40.5 29.0 41.0 64-65 34.99097397962494 37.0 35.0 40.0 29.0 41.0 66-67 34.55858730055732 37.0 35.0 39.5 28.0 41.0 68-69 34.131035551806434 36.0 35.0 39.0 28.0 41.0 70-71 33.70188970360277 36.0 34.0 39.0 26.5 40.5 72-73 33.27057008341805 35.0 34.0 37.5 26.0 39.5 74-75 32.924662758924 35.0 34.0 37.0 26.0 39.0 76-77 32.618662715664854 35.0 34.0 36.5 26.0 39.0 78-79 32.305707323052076 35.0 34.0 36.0 25.0 37.5 80-81 32.04238891412339 35.0 34.0 36.0 25.0 37.0 82-83 31.824662758924 35.0 33.0 35.0 25.0 37.0 84-85 31.709153634849567 35.0 33.0 35.0 25.0 36.0 86-87 31.60992725253967 35.0 33.0 35.0 25.0 36.0 88-89 31.459274399959625 35.0 33.0 35.0 24.0 36.0 90-91 31.345642722730517 35.0 33.0 35.0 24.0 35.5 92-93 31.22793819710308 35.0 33.0 35.0 23.0 35.0 94-95 31.090100865903864 35.0 33.0 35.0 20.0 35.0 96-97 30.96749363730092 35.0 33.0 35.0 19.5 35.0 98-99 30.83909617228675 35.0 33.0 35.0 18.5 35.0 100 30.732400377796523 35.0 33.0 35.0 16.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10718044274265992 1101 2 0.1227214346578549 1101 3 0.06702288764397935 1101 4 0.0734226182066493 1101 5 0.12497958657323949 1101 6 0.0730545484070717 1101 7 0.09180967007092988 1101 8 0.16972345206164619 1101 9 0.05730124425711125 1101 10-11 0.18234211744520223 1101 12-13 0.18867117701714164 1101 14-15 0.23807231533050555 1101 16-17 0.2528320861601969 1101 18-19 0.2327879308334957 1101 20-21 0.15502170649163816 1101 22-23 0.17163839249756307 1101 24-25 0.19702901115675076 1101 26-27 0.2981809825904236 1101 28-29 0.312010461766576 1101 30-31 0.3835365147931782 1101 32-33 0.38549743043401463 1101 34-35 0.35846263593418826 1101 36-37 0.3812934119047142 1101 38-39 0.3174876170707819 1101 40-41 0.3004546982923202 1101 42-43 0.19411074279934581 1101 44-45 0.23935272416877496 1101 46-47 0.2185939417129319 1101 48-49 0.20910837532623106 1101 50-51 0.38366355466466473 1101 52-53 0.49809947167980795 1101 54-55 0.6259199470467252 1101 56-57 0.5370450041057495 1101 58-59 0.49515887697967287 1101 60-61 0.6067839895308182 1101 62-63 0.5002230162656076 1101 64-65 0.4274040458238204 1101 66-67 0.3982611520251709 1101 68-69 0.41975820468936575 1101 70-71 0.24010518882660392 1101 72-73 0.3772395995626354 1101 74-75 0.4438456635103023 1101 76-77 0.5145159343272496 1101 78-79 0.39326197984306077 1101 80-81 0.27757857806967223 1101 82-83 0.3112980300901462 1101 84-85 0.22650787823250695 1101 86-87 0.4600312061529692 1101 88-89 0.5718933070758858 1101 90-91 0.5228976113484372 1101 92-93 0.5019157300706141 1101 94-95 0.5714535948887445 1101 96-97 0.6377070027071738 1101 98-99 0.6579006129283052 1101 100 0.723114721192907 1102 1 0.1898999501678702 1102 2 0.12086670889230788 1102 3 0.15779972754455684 1102 4 0.1582883083034261 1102 5 0.20197720192582835 1102 6 0.13182790596539462 1102 7 0.1595601526781465 1102 8 0.1709220843591126 1102 9 0.2768969897433138 1102 10-11 0.2542285123377681 1102 12-13 0.24453185809263545 1102 14-15 0.2845710473037073 1102 16-17 0.35443699172059695 1102 18-19 0.43576558580403457 1102 20-21 0.3702411823738885 1102 22-23 0.45218837546437385 1102 24-25 0.3776515429205105 1102 26-27 0.40918064192650405 1102 28-29 0.4308409751597111 1102 30-31 0.3681782499080555 1102 32-33 0.21619251099471626 1102 34-35 0.33610309671367844 1102 36-37 0.3930204517876277 1102 38-39 0.3959280071940512 1102 40-41 0.2386065334614571 1102 42-43 0.3018494914272267 1102 44-45 0.38610695407907514 1102 46-47 0.4476203999437516 1102 48-49 0.37318347279396136 1102 50-51 0.1349195951405875 1102 52-53 0.17454887047108514 1102 54-55 0.177017011028461 1102 56-57 0.15988462710434703 1102 58-59 0.16959772780673177 1102 60-61 0.19756335286781024 1102 62-63 0.30197491199721327 1102 64-65 0.17360690487727481 1102 66-67 0.15585164876363677 1102 68-69 0.1154147397588261 1102 70-71 0.1538989231339798 1102 72-73 0.0826252437328705 1102 74-75 0.014975027478151048 1102 76-77 0.062003222516310075 1102 78-79 0.09456407303356684 1102 80-81 0.05251454587062199 1102 82-83 0.008663562472140285 1102 84-85 0.17545939040178027 1102 86-87 0.20155498323259025 1102 88-89 0.23888612789928132 1102 90-91 0.23269863838705973 1102 92-93 0.3247225483958118 1102 94-95 0.27969829251797407 1102 96-97 0.38389156684661785 1102 98-99 0.42008565652067276 1102 100 0.40214244114193676 1103 1 0.24408498512387666 1103 2 0.2648936640511792 1103 3 0.23917237439793126 1103 4 0.14130632341286997 1103 5 0.04694160021882254 1103 6 0.07786123597237804 1103 7 0.17882792564902417 1103 8 0.24203275195715435 1103 9 0.13019447678523477 1103 10-11 0.18052270629516443 1103 12-13 0.24314594498076048 1103 14-15 0.29120711156875245 1103 16-17 0.31388685289447693 1103 18-19 0.3798160210664463 1103 20-21 0.2873869409866927 1103 22-23 0.30590909137272604 1103 24-25 0.3067345861767308 1103 26-27 0.2461618050134362 1103 28-29 0.3139681727506556 1103 30-31 0.4477352977546474 1103 32-33 0.31903360352393406 1103 34-35 0.2907172305620094 1103 36-37 0.4161418967531958 1103 38-39 0.496001969520222 1103 40-41 0.41567997335532425 1103 42-43 0.33929790857474273 1103 44-45 0.42478514164987047 1103 46-47 0.34386593138654575 1103 48-49 0.27115890086559347 1103 50-51 0.2533420843837604 1103 52-53 0.3560348088240701 1103 54-55 0.4805883347779982 1103 56-57 0.49760189143748335 1103 58-59 0.42643307945063214 1103 60-61 0.3335219574452637 1103 62-63 0.4232760619352902 1103 64-65 0.3063763243904205 1103 66-67 0.36431622609242 1103 68-69 0.4883457023843718 1103 70-71 0.5011015622976203 1103 72-73 0.6087750350534478 1103 74-75 0.45478671704771045 1103 76-77 0.5009502572727342 1103 78-79 0.571078686463018 1103 80-81 0.49273009322118355 1103 82-83 0.5861643402505727 1103 84-85 0.5909832608620498 1103 86-87 0.5216545388716369 1103 88-89 0.45281100330798196 1103 90-91 0.5809220750524133 1103 92-93 0.6640523088400556 1103 94-95 0.6233959392245971 1103 96-97 0.5199769639832219 1103 98-99 0.49253703803126214 1103 100 0.6243440530510433 1104 1 0.23098282726468256 1104 2 0.21328329658403078 1104 3 0.2937809371906326 1104 4 0.18898626495470694 1104 5 0.13487463904700547 1104 6 0.1639519568457075 1104 7 0.19487956369554382 1104 8 0.15633728800082025 1104 9 0.15917887686937604 1104 10-11 0.2524677689051842 1104 12-13 0.22301828917535715 1104 14-15 0.3512014650413633 1104 16-17 0.38336681879388124 1104 18-19 0.4752666914531858 1104 20-21 0.3784028730512148 1104 22-23 0.4121645579862445 1104 24-25 0.43575607619565915 1104 26-27 0.5170893855318042 1104 28-29 0.5576662053233647 1104 30-31 0.5818480732996463 1104 32-33 0.68557239295275 1104 34-35 0.6204816264389308 1104 36-37 0.7297406168736629 1104 38-39 0.6796065576005148 1104 40-41 0.7376361075876687 1104 42-43 0.663986519196861 1104 44-45 0.6733535808832798 1104 46-47 0.7702966617814795 1104 48-49 0.8676790899689095 1104 50-51 1.083477933189691 1104 52-53 1.133913707419083 1104 54-55 1.1023817161621707 1104 56-57 1.0819261349688887 1104 58-59 0.9240515734885619 1104 60-61 0.928937198640547 1104 62-63 0.848188319328365 1104 64-65 0.8753265131615819 1104 66-67 0.9110833123735773 1104 68-69 0.8312952932879227 1104 70-71 0.9717429413788992 1104 72-73 0.9724955430194839 1104 74-75 0.9269969450124051 1104 76-77 0.8082226523967861 1104 78-79 0.8228578961706177 1104 80-81 0.8503529051918264 1104 82-83 0.9725816739824786 1104 84-85 0.9145602806027533 1104 86-87 1.0395949956867696 1104 88-89 1.0342228565868403 1104 90-91 0.9390431100276686 1104 92-93 0.9409247969657386 1104 94-95 1.0201292570576648 1104 96-97 1.110888269216776 1104 98-99 1.3076293200033327 1104 100 1.2978773398835663 1105 1 0.11872287511928903 1105 2 0.06525017029003521 1105 3 0.16626578116727586 1105 4 0.08876434853908677 1105 5 0.02956352215036162 1105 6 0.12219075874501328 1105 7 0.1267673409360981 1105 8 0.11817138378440717 1105 9 0.02443594038210506 1105 10-11 0.17855658506169192 1105 12-13 0.13727053003525214 1105 14-15 0.17471282740979888 1105 16-17 0.18934182530260557 1105 18-19 0.21739113821183764 1105 20-21 0.23319639160416727 1105 22-23 0.20361581695898678 1105 24-25 0.21464550231237212 1105 26-27 0.199206517727049 1105 28-29 0.23308555296349454 1105 30-31 0.24416009521853965 1105 32-33 0.2859585771061859 1105 34-35 0.1315681490458971 1105 36-37 0.21183233406039648 1105 38-39 0.22390925655044214 1105 40-41 0.2672073997858817 1105 42-43 0.24331233960572973 1105 44-45 0.19197162429954773 1105 46-47 0.102749767991412 1105 48-49 0.07986341696708621 1105 50-51 -0.05305955084844527 1105 52-53 0.06405032858668136 1105 54-55 0.05716779617605994 1105 56-57 0.10708079459373465 1105 58-59 0.097505907057446 1105 60-61 0.20498758234518988 1105 62-63 0.1727503753405628 1105 64-65 0.1257728255058339 1105 66-67 0.1552781525069733 1105 68-69 0.19682820262452339 1105 70-71 0.20728118166850606 1105 72-73 0.10237553458913595 1105 74-75 -0.06501592366018372 1105 76-77 -0.001149286954330364 1105 78-79 0.011074630404010577 1105 80-81 0.10252867787006892 1105 82-83 0.14672717180670602 1105 84-85 0.1567405164044402 1105 86-87 -0.005045536912625437 1105 88-89 0.0351185806666372 1105 90-91 0.06599417683814579 1105 92-93 -0.020924795774245553 1105 94-95 -0.028642764117382313 1105 96-97 0.09485821751892942 1105 98-99 -5.927451830594066E-4 1105 100 0.022818761388379016 1106 1 0.05711683818130808 1106 2 0.03593528227949605 1106 3 0.04434558992920046 1106 4 0.107535107416588 1106 5 0.07558610149759915 1106 6 0.10216953636763293 1106 7 0.08345475661856483 1106 8 0.20626200096901215 1106 9 0.20637443493450291 1106 10-11 0.17765128104522176 1106 12-13 0.11865164133044459 1106 14-15 0.28132568864385377 1106 16-17 0.2601784129967797 1106 18-19 0.19348144771142017 1106 20-21 0.17119884519500772 1106 22-23 0.1902325045324602 1106 24-25 0.21250649968521884 1106 26-27 0.22638733396019006 1106 28-29 0.2489658477099681 1106 30-31 0.27662974090739567 1106 32-33 0.18688991600940597 1106 34-35 0.09297833263129007 1106 36-37 0.05952134339352 1106 38-39 0.10631933757416334 1106 40-41 0.01711568598029345 1106 42-43 0.030375564933734722 1106 44-45 0.1161824911034941 1106 46-47 0.13386244382551382 1106 48-49 -0.01588480989934027 1106 50-51 0.02044857124728594 1106 52-53 0.07596622012376741 1106 54-55 0.061703191787998435 1106 56-57 -0.174567371524617 1106 58-59 -0.11363899681069967 1106 60-61 -0.120093480824913 1106 62-63 -5.788555257666417E-4 1106 64-65 -0.052633208276404275 1106 66-67 -0.08832850769042011 1106 68-69 -0.010625467111097464 1106 70-71 -0.17358455720946608 1106 72-73 -0.042067792196029075 1106 74-75 -0.04684081143404484 1106 76-77 -0.22445662484770423 1106 78-79 -0.12284133213731252 1106 80-81 -0.11477978820173718 1106 82-83 -0.1110339857201339 1106 84-85 -0.1087551720605262 1106 86-87 -0.18939521699909534 1106 88-89 0.03170544646073736 1106 90-91 0.05162074977922515 1106 92-93 -0.1139876818597827 1106 94-95 -0.018408292349455735 1106 96-97 -0.05481378235484158 1106 98-99 0.07913317846371726 1106 100 0.07620408560161351 1107 1 0.04555769939029375 1107 2 0.01972475713132127 1107 3 -4.01819085908528E-4 1107 4 -0.07786497466669573 1107 5 0.04149195714192899 1107 6 0.09548178193716694 1107 7 0.1158443786287151 1107 8 0.027609756804508834 1107 9 0.1293150502633722 1107 10-11 0.07440473908106782 1107 12-13 0.15505021855344125 1107 14-15 0.09199799514728824 1107 16-17 0.0457968292330051 1107 18-19 0.1314535652753932 1107 20-21 0.0571737876072973 1107 22-23 0.16699821959991823 1107 24-25 0.15140575169200332 1107 26-27 0.1006067107927322 1107 28-29 0.22454930459959854 1107 30-31 0.20782444098911412 1107 32-33 0.16816143052002985 1107 34-35 0.225764228355942 1107 36-37 0.04435858184546504 1107 38-39 0.27544650011756744 1107 40-41 0.09478283824479661 1107 42-43 0.2744194425187061 1107 44-45 0.24839759368911274 1107 46-47 0.28428053246807394 1107 48-49 0.16979674021768432 1107 50-51 0.2294610020588408 1107 52-53 0.2777963631779343 1107 54-55 0.29720689477959894 1107 56-57 0.3693471879062713 1107 58-59 0.40382311361720014 1107 60-61 0.3615080306907785 1107 62-63 0.1860885259803453 1107 64-65 0.31018417119611996 1107 66-67 0.2516760956291506 1107 68-69 0.24306874305909076 1107 70-71 0.315586087292246 1107 72-73 0.35262480219051184 1107 74-75 0.28482928769446403 1107 76-77 0.3286326832493245 1107 78-79 0.39778764030562996 1107 80-81 0.3660101340409909 1107 82-83 0.2455356353286824 1107 84-85 0.24465729276752413 1107 86-87 0.4145385876352847 1107 88-89 0.41253446918893744 1107 90-91 0.45331168401276045 1107 92-93 0.49034406033953104 1107 94-95 0.4356638891803257 1107 96-97 0.4947777423607356 1107 98-99 0.44554724798308953 1107 100 0.4508579122727667 1108 1 0.07362727397185864 1108 2 0.015159095608801465 1108 3 -0.027248828956736304 1108 4 0.047017241659951026 1108 5 0.030217950997588616 1108 6 0.101325385666037 1108 7 0.08870684989631883 1108 8 0.12729521262945553 1108 9 0.17887024117941763 1108 10-11 0.09577406267477073 1108 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-0.22282986736240673 2215 6 -0.21961044128653384 2215 7 -0.20783397315305052 2215 8 -0.17891226047721887 2215 9 -0.24727898842289875 2215 10-11 -0.2326650446272609 2215 12-13 -0.3503044719364965 2215 14-15 -0.4596218467070159 2215 16-17 -0.420111001566454 2215 18-19 -0.3846403610995708 2215 20-21 -0.3186148794707009 2215 22-23 -0.33101856170010535 2215 24-25 -0.45075773376865413 2215 26-27 -0.518255973703468 2215 28-29 -0.5051173724925846 2215 30-31 -0.6025105452634136 2215 32-33 -0.5263400778635088 2215 34-35 -0.43572735113914973 2215 36-37 -0.4542435688450368 2215 38-39 -0.49442762591563394 2215 40-41 -0.45453604341889076 2215 42-43 -0.41462785902436394 2215 44-45 -0.5815360715549431 2215 46-47 -0.5314741853177694 2215 48-49 -0.5450395127358192 2215 50-51 -0.5784752847302244 2215 52-53 -0.6467787730883856 2215 54-55 -0.5550507757985343 2215 56-57 -0.6089355010823922 2215 58-59 -0.4532610999619422 2215 60-61 -0.48766889800717195 2215 62-63 -0.42515866979105965 2215 64-65 -0.4381123221320138 2215 66-67 -0.4294027895602568 2215 68-69 -0.3640485241650495 2215 70-71 -0.4468179149710352 2215 72-73 -0.3122496223917395 2215 74-75 -0.4715152350677698 2215 76-77 -0.43936555957625245 2215 78-79 -0.5828621161636462 2215 80-81 -0.6065652922444116 2215 82-83 -0.5516958908825025 2215 84-85 -0.5847514237274538 2215 86-87 -0.6787103653105433 2215 88-89 -0.6611264672314867 2215 90-91 -0.5481711096102302 2215 92-93 -0.5499071047126023 2215 94-95 -0.46890206314850147 2215 96-97 -0.6204892031412719 2215 98-99 -0.7375724332233915 2215 100 -0.6568052732171807 2216 1 -0.42931017270910843 2216 2 -0.4166063697943514 2216 3 -0.4771208632653767 2216 4 -0.37956734250919055 2216 5 -0.5015590428462247 2216 6 -0.2752649627234405 2216 7 -0.3391666139389642 2216 8 -0.42655182801983216 2216 9 -0.5359622505777466 2216 10-11 -0.5236087046121227 2216 12-13 -0.5918977157516565 2216 14-15 -0.7693989490373028 2216 16-17 -0.7033879051099845 2216 18-19 -0.6505817767178002 2216 20-21 -0.6924320982515582 2216 22-23 -0.7271467086227545 2216 24-25 -0.6832561627120128 2216 26-27 -0.9112763140776963 2216 28-29 -0.9125712347269541 2216 30-31 -0.8796164024190958 2216 32-33 -0.944431276098797 2216 34-35 -0.9287845632510283 2216 36-37 -0.8252466724834733 2216 38-39 -0.9614226113593105 2216 40-41 -0.9935508305888092 2216 42-43 -1.0162268099432765 2216 44-45 -0.9006734652681487 2216 46-47 -0.8119385768379104 2216 48-49 -1.00458288031178 2216 50-51 -0.898003839486357 2216 52-53 -1.0136057516578845 2216 54-55 -1.123723585617313 2216 56-57 -1.0959245870480387 2216 58-59 -1.1357592082814918 2216 60-61 -1.0792919598362687 2216 62-63 -1.2230201090072015 2216 64-65 -1.328917793256636 2216 66-67 -1.2226103742370071 2216 68-69 -1.2419979425534748 2216 70-71 -1.4114370780146572 2216 72-73 -1.2773144525252391 2216 74-75 -1.471897098020328 2216 76-77 -1.282763274169767 2216 78-79 -1.3804612851067404 2216 80-81 -1.4384551772045882 2216 82-83 -1.392921379192554 2216 84-85 -1.6479210465456084 2216 86-87 -1.628321769258065 2216 88-89 -1.9596576895776856 2216 90-91 -1.9767822955335674 2216 92-93 -1.9433192473773673 2216 94-95 -1.710002828798146 2216 96-97 -1.9563785494534578 2216 98-99 -2.1468862341544828 2216 100 -2.060662377599467 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 0.0 4 5.0 5 17.0 6 45.0 7 96.0 8 215.0 9 318.0 10 518.0 11 929.0 12 1523.0 13 2255.0 14 2418.0 15 3297.0 16 4670.0 17 7094.0 18 8470.0 19 3935.0 20 2041.0 21 1680.0 22 1829.0 23 2076.0 24 2525.0 25 3371.0 26 4195.0 27 5038.0 28 5201.0 29 6725.0 30 8243.0 31 10462.0 32 13228.0 33 17393.0 34 24365.0 35 37083.0 36 69448.0 37 166494.0 38 230809.0 39 45460.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.527271520771365 21.05381599332962 13.565652165132269 22.853260320766747 2 17.337422816308596 20.540256932078353 38.105328276404336 24.016991975208715 3 17.322608694398262 27.254099247131503 30.82160866700025 24.60168339146999 4 12.252752402719597 18.87885102057002 38.49124350202239 30.37715307468799 5 11.423619272292584 39.247692478510956 36.60094622440313 12.72774202479333 6 28.58337635221069 39.62889368905859 17.36208244051138 14.425647518219337 7 26.346549001795257 33.5408330269144 23.269670293224898 16.84294767806545 8 25.96151073119805 33.728723619017956 20.19432091502137 20.115444734762622 9 26.049935471777015 15.223757921830728 21.52589420255373 37.20041240383853 10-11 24.727645283486833 26.06926714050259 28.64493885267106 20.55814872333952 12-13 25.370766912522804 25.400327327522188 27.410435547480517 21.818470212474494 14-15 22.853206266533142 25.773709486822877 26.839040308236488 24.5340439384075 16-17 21.299231644951764 28.703616250741714 27.5669814252312 22.43017067907532 18-19 20.874471696181505 26.9334369633147 31.229875427726018 20.96221591277778 20-21 21.58825946834512 26.364429447941223 31.458986726652675 20.588324357060976 22-23 21.594650851089447 26.03066935739124 31.26345091915065 21.111228872368667 24-25 21.274308489621937 27.344833751993562 30.20200956910201 21.17884818928249 26-27 20.53912545683557 27.006165162326685 31.005481666374667 21.449227714463078 28-29 21.91425561812942 26.029590845742163 31.72735354362671 20.32879999250171 30-31 21.869387572648378 26.612323529602616 30.907094432616265 20.611194465132744 32-33 20.916372864981003 26.110354076092833 31.42524459440948 21.548028464516687 34-35 21.930078803740475 26.009488172229073 31.162877886646623 20.89755513738383 36-37 22.34716904952451 25.85656709853712 30.036337680877295 21.759926171061075 38-39 21.29820690848528 26.091536348495666 30.94268884418777 21.667567898831283 40-41 21.107680816272385 26.053289574242893 31.395513155124632 21.443516454360097 42-43 21.678743177672512 26.081226252532463 30.480392793026628 21.759637776768397 44-45 20.849897980518968 25.996726724778117 30.70577293275366 22.447602361949258 46-47 22.58545483384884 25.903431171097125 29.275409339649165 22.23570465540487 48-49 22.438301646010427 25.866156208768626 28.982905428301574 22.71263671691937 50-51 22.201529931722654 26.208696529895676 28.264731540962806 23.32504199741887 52-53 21.970670300434755 26.682888845629744 28.622989351040744 22.723451502894758 54-55 21.862738736400406 27.45319000136987 28.3432468871441 22.340824375085617 56-57 22.328856011939525 26.707690754799962 27.988666104297792 22.974787128962717 58-59 22.32734194190297 25.75433132178314 28.63142488410154 23.286901852212345 60-61 22.505281220484648 26.61071817388734 28.52969379735975 22.354306808268266 62-63 21.387537040641966 26.52210902746235 28.767330694525555 23.323023237370133 64-65 21.568216065004073 27.140426390961725 27.837907987800918 23.453449556233284 66-67 21.577805175235582 26.20884072704201 28.15384393542852 24.059510162293886 68-69 21.791511804381447 27.116010627368 27.830437964361547 23.26203960388901 70-71 22.50450436160633 26.373100291350543 28.275927206344104 22.846468140699024 72-73 22.014024675122425 25.944495394323226 27.801978970197204 24.239500960357148 74-75 21.616136272365928 26.09714438563591 28.69442900329996 23.5922903386982 76-77 21.809169496535663 26.478705686414468 27.902868802226404 23.809256014823465 78-79 21.708736184110915 27.06119005904873 27.619233015378626 23.61084074146173 80-81 21.64608252402685 26.76176468467689 27.781743199302085 23.810409591994176 82-83 21.633800460999275 26.76751836895371 27.72030307421771 23.878378095829305 84-85 21.73945017628101 26.415258942025538 27.779003453521657 24.066287428171794 86-87 22.399265167633633 25.95230078522952 27.681449592388656 23.966984454748182 88-89 21.73854109286812 26.536241957207007 28.021292672586583 23.703924277338288 90-91 21.882070309063984 25.85564709054754 28.10235608473772 24.159926515650753 92-93 21.855790070210197 26.06061523686749 27.778306505222144 24.305288187700167 94-95 22.074595217375318 25.493248275568696 28.092638749397068 24.339517757658914 96-97 22.00782417100702 26.014142917293924 27.688279028453106 24.28975388324595 98-99 21.014616575353624 25.808893666631338 27.793915876644842 25.38257388137019 100 21.097196086489447 26.024700971167782 28.07777994073497 24.8003230016078 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 61.0 1 45.5 2 89.5 3 146.5 4 97.0 5 61.5 6 115.0 7 286.0 8 498.5 9 559.0 10 397.0 11 222.5 12 207.5 13 226.5 14 240.5 15 278.0 16 327.5 17 380.0 18 428.5 19 472.0 20 514.5 21 616.0 22 789.5 23 1040.0 24 1282.5 25 1572.5 26 1837.0 27 2116.5 28 2650.0 29 3325.0 30 4437.0 31 5709.5 32 7043.0 33 8037.5 34 8815.0 35 11793.0 36 15495.0 37 18436.0 38 22103.5 39 24436.0 40 26189.0 41 28332.0 42 31495.0 43 36796.0 44 39322.5 45 38457.5 46 38726.0 47 37817.0 48 34616.0 49 33694.0 50 34932.0 51 32193.5 52 25324.5 53 18959.0 54 15149.0 55 14698.5 56 12320.0 57 8603.5 58 7321.0 59 6502.5 60 5361.0 61 3878.5 62 2759.0 63 2296.5 64 1994.5 65 1751.5 66 1520.0 67 1151.0 68 820.5 69 648.5 70 452.0 71 295.5 72 271.5 73 242.5 74 191.0 75 190.5 76 240.0 77 208.5 78 126.5 79 145.5 80 138.5 81 97.5 82 76.5 83 27.0 84 1.5 85 0.5 86 1.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04051939812111119 2 0.2363391228487588 3 0.0020187600487386356 4 0.0014419714633847397 5 2.883942926769479E-4 6 1.4419714633847396E-4 7 0.0 8 4.3259143901542185E-4 9 0.0 10-11 8.651828780308437E-4 12-13 0.0 14-15 2.1629571950771093E-4 16-17 7.209857316923698E-5 18-19 2.883942926769479E-4 20-21 0.0 22-23 2.1629571950771093E-4 24-25 0.0018745629024001615 26-27 2.1629571950771093E-4 28-29 5.046900121846589E-4 30-31 7.209857316923698E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.4419714633847396E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.604928658461849E-4 70-71 7.209857316923698E-5 72-73 4.3259143901542185E-4 74-75 2.1629571950771093E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.1629571950771093E-4 84-85 0.0 86-87 5.046900121846589E-4 88-89 0.0024513514877540572 90-91 0.0020187600487386356 92-93 8.651828780308437E-4 94-95 7.930843048616068E-4 96-97 4.3259143901542185E-4 98-99 2.1629571950771093E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 693495.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.280077626285003 #Duplication Level Percentage of deduplicated Percentage of total 1 67.70658167071393 14.408013137631977 2 10.305733239374195 4.3861360665933695 3 4.631792596892914 2.9569471803220027 4 2.9098855983492613 2.4769036566592426 5 2.0577880459310585 2.189494467792713 6 1.5756404786503697 2.0117851018078037 7 1.1963169896747903 1.7820402884146194 8 0.9575281596879268 1.6301018854010325 9 0.8083873113476296 1.5482290263823244 >10 6.51965506373647 27.79257766152395 >50 0.757782725838557 11.37097881747 >100 0.5444658727204646 22.5312718692043 >500 0.024379068927781997 3.3195257275799235 >1k 0.004063178154630333 1.595995113216742 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2920 0.42105566730834393 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2854 0.4115386556500047 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 1846 0.2661879321408229 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1333 0.1922147960691858 No Hit CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG 1065 0.15356996085047475 No Hit CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA 1057 0.15241638367976698 No Hit AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA 961 0.13857345763127346 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 957 0.13799666904591956 TruSeq Adapter, Index 3 (95% over 21bp) GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT 884 0.12747027736321098 No Hit GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA 860 0.1240095458510876 No Hit GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA 825 0.11896264572924102 No Hit CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA 764 0.1101666198025941 No Hit GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT 748 0.10785946546117851 No Hit TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT 721 0.10396614251003973 No Hit GTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGT 717 0.10338935392468582 No Hit GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA 702 0.10122639672960872 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.4419714633847396E-4 0.0 5 0.0 0.0 0.0 1.4419714633847396E-4 0.0 6 0.0 0.0 0.0 1.4419714633847396E-4 0.0 7 0.0 0.0 0.0 1.4419714633847396E-4 0.0 8 0.0 0.0 0.0 1.4419714633847396E-4 0.0 9 0.0 0.0 0.0 1.4419714633847396E-4 0.0 10-11 0.0 0.0 0.0 1.4419714633847396E-4 0.0 12-13 0.0 0.0 0.0 1.4419714633847396E-4 0.0 14-15 0.0 0.0 0.0 2.883942926769479E-4 0.0 16-17 0.0 0.0 0.0 2.883942926769479E-4 0.0 18-19 0.0 0.0 0.0 5.046900121846589E-4 0.0 20-21 0.0 0.0 0.0 7.209857316923698E-4 0.0 22-23 0.0 0.0 0.0 0.0014419714633847397 0.0 24-25 0.0 0.0 0.0 0.003749125804800323 0.0 26-27 0.0 0.0 0.0 0.008868124499816148 0.0 28-29 0.0 0.0 0.0 0.01838513615815543 0.0 30-31 0.0 0.0 0.0 0.048017649730711834 0.0 32-33 0.0 0.0 0.0 0.11081550696111725 0.0 34-35 0.0 0.0 0.0 0.19913625909343252 0.0 36-37 0.0 0.0 0.0 0.33360009805405955 0.0 38-39 0.0 0.0 0.0 0.5408834959156159 0.0 40-41 0.0 0.0 0.0 0.7626587069841888 0.0 42-43 0.0 0.0 0.0 0.9527105458582974 0.0 44-45 0.0 0.0 0.0 1.1983503846458878 0.0 46-47 0.0 0.0 0.0 1.4855911001521278 0.0 48-49 0.0 0.0 0.0 1.7685059012682138 0.0 50-51 0.0 0.0 0.0 2.057260686811008 0.0 52-53 0.0 0.0 0.0 2.336137967829617 0.0 54-55 0.0 0.0 0.0 2.6442872695549355 0.0 56-57 0.0 0.0 0.0 3.014657639925306 0.0 58-59 0.0 0.0 0.0 3.4134348481243553 0.0 60-61 0.0 0.0 0.0 3.842637654200823 0.0 62-63 0.0 0.0 0.0 4.260737280009229 0.0 64-65 0.0 0.0 0.0 4.703855110707359 0.0 66-67 0.0 0.0 0.0 5.193476521099647 0.0 68-69 0.0 0.0 0.0 5.763271544856128 0.0 70-71 0.0 0.0 0.0 6.366231912270456 0.0 72-73 0.0 0.0 0.0 6.982097924282078 0.0 74-75 0.0 0.0 0.0 7.632787547134442 0.0 76-77 0.0 0.0 0.0 8.28210729709659 0.0 78-79 0.0 0.0 0.0 9.025515685044592 0.0 80-81 0.0 0.0 0.0 9.832154521662016 0.0 82-83 0.0 0.0 0.0 10.67642881347378 0.0 84-85 0.0 0.0 0.0 11.532238876992626 0.0 86-87 0.0 0.0 0.0 12.419411819840086 0.0 88 0.0 0.0 0.0 13.125833639752269 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1855 0.0 58.939735 1 GTATAAC 40 7.903749E-6 58.907978 1 TATAACG 25 0.005190201 56.523052 2 GGACCGT 45 1.6138387E-5 52.21918 6 AACACGT 45 0.001013968 41.775345 4 GTATCAA 3405 0.0 37.78415 1 CAACGAG 50 0.0016792625 37.701107 1 GCTCTAG 50 0.0016792625 37.701107 1 AACGAGA 55 0.0026873944 34.256397 2 GAGTTAC 100 1.4107372E-6 32.898083 7 CTTCGTA 60 0.004105532 31.41759 1 GTACATT 30 0.0041624624 31.331509 14-15 TATAAGG 60 0.0041610403 31.331509 3 AAATACG 200 0.0 30.54822 5 ATCAACG 4310 0.0 29.55048 3 CAACGCA 4350 0.0 29.386793 5 TCAACGC 4350 0.0 29.386793 4 ACTACGC 80 4.780581E-4 29.37329 8 TAACTCG 65 0.006154259 28.92139 5 ATGGGAC 115 4.195139E-6 28.607029 5 >>END_MODULE