##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033016_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 168689 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.508219267409256 33.0 31.0 34.0 16.0 34.0 2 29.512214785789233 33.0 31.0 34.0 16.0 34.0 3 29.635726099508563 34.0 31.0 34.0 18.0 34.0 4 32.57108643717136 37.0 35.0 37.0 17.0 37.0 5 33.03920231906052 37.0 35.0 37.0 23.0 37.0 6 32.83408521006112 37.0 35.0 37.0 17.0 37.0 7 32.947346892802734 37.0 35.0 37.0 19.0 37.0 8 32.68007398229879 37.0 35.0 37.0 16.0 37.0 9 33.97162826266087 39.0 37.0 39.0 13.0 39.0 10-11 34.182495598408906 39.0 37.0 39.0 15.0 39.0 12-13 34.127717278542164 39.0 37.0 39.0 14.5 39.0 14-15 35.37167213037009 40.0 37.0 41.0 13.0 41.0 16-17 35.451126036671035 40.0 37.0 41.0 17.0 41.0 18-19 35.47415361997522 40.0 36.5 41.0 17.5 41.0 20-21 35.532064924209635 40.0 36.0 41.0 20.0 41.0 22-23 35.506227436288086 40.0 36.0 41.0 23.0 41.0 24-25 35.52539584679499 40.0 36.0 41.0 24.0 41.0 26-27 35.29267172133334 40.0 36.0 41.0 24.0 41.0 28-29 35.12091481957923 40.0 35.5 41.0 23.0 41.0 30-31 35.02484157236097 40.0 36.0 41.0 18.5 41.0 32-33 34.82514568229108 40.0 35.5 41.0 6.0 41.0 34-35 34.58529898215059 40.0 35.0 41.0 2.0 41.0 36-37 34.353028353953135 40.0 35.0 41.0 2.0 41.0 38-39 34.12536087118899 40.0 35.0 41.0 2.0 41.0 40-41 33.98359999762877 40.0 35.0 41.0 2.0 41.0 42-43 33.79891101375905 40.0 35.0 41.0 2.0 41.0 44-45 33.65151551079205 40.0 35.0 41.0 2.0 41.0 46-47 33.47763932443728 40.0 34.0 41.0 2.0 41.0 48-49 33.30557119907048 40.0 34.0 41.0 2.0 41.0 50-51 33.135385235551816 40.0 34.0 41.0 2.0 41.0 52-53 32.96250200072322 39.0 34.0 41.0 2.0 41.0 54-55 32.78716454540604 39.0 33.0 41.0 2.0 41.0 56-57 32.60109728553729 39.0 33.0 41.0 2.0 41.0 58-59 32.3876571679244 38.5 33.0 41.0 2.0 41.0 60-61 32.15700786654732 38.0 33.0 41.0 2.0 41.0 62-63 31.88500139309617 37.5 32.5 40.0 2.0 41.0 64-65 31.602113949338722 37.0 32.0 40.0 2.0 41.0 66-67 31.288522073164224 36.5 32.0 40.0 2.0 41.0 68-69 30.97956594680151 36.0 31.0 39.0 2.0 41.0 70-71 30.644988707028908 35.0 31.0 39.0 2.0 41.0 72-73 30.3235154633675 35.0 31.0 38.5 2.0 40.5 74-75 30.005101103213605 35.0 31.0 37.5 2.0 40.0 76-77 29.668238592913585 35.0 30.0 37.0 2.0 39.0 78-79 29.330477980188395 35.0 30.0 36.5 2.0 39.0 80-81 29.03239096799436 35.0 30.0 36.0 2.0 39.0 82-83 28.837683547830622 35.0 30.0 36.0 2.0 37.0 84-85 28.63786020428125 35.0 30.0 35.0 2.0 37.0 86-87 28.497003361215018 35.0 30.0 35.0 2.0 37.0 88-89 28.29181215135545 35.0 29.0 35.0 2.0 36.0 90-91 28.072556598236993 35.0 29.0 35.0 2.0 36.0 92-93 27.852032438392545 35.0 29.0 35.0 2.0 36.0 94-95 27.65429281103095 35.0 28.5 35.0 2.0 35.5 96-97 27.463687614485828 35.0 28.0 35.0 2.0 35.0 98-99 27.27431249221941 34.5 27.0 35.0 2.0 35.0 100 27.1248629134087 34.0 27.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.1700123134603544 1101 2 1.063143770498705 1101 3 1.2154131615438857 1101 4 1.016726718358676 1101 5 0.9869800862476765 1101 6 1.3956073676199665 1101 7 0.851029763288011 1101 8 0.9958764565147149 1101 9 1.1409385108779304 1101 10-11 1.346611476488107 1101 12-13 1.0864362361100675 1101 14-15 1.2854576190528277 1101 16-17 1.3736177950825805 1101 18-19 0.9243850987193909 1101 20-21 1.1458071876321085 1101 22-23 1.1158775110022177 1101 24-25 1.1845629801051913 1101 26-27 1.150006531617855 1101 28-29 1.1666312905416163 1101 30-31 1.3889950957826258 1101 32-33 1.3534140952393159 1101 34-35 1.5767881442440768 1101 36-37 1.6483115531701458 1101 38-39 1.4726533037652914 1101 40-41 1.480667007729167 1101 42-43 1.542451263367468 1101 44-45 1.7914786860961982 1101 46-47 1.786376222052752 1101 48-49 1.7399986107524796 1101 50-51 1.4294160025980673 1101 52-53 1.401347197790173 1101 54-55 1.4193067798729544 1101 56-57 1.4176982613067608 1101 58-59 1.550453026900776 1101 60-61 1.6799875269769373 1101 62-63 1.4993834141474167 1101 64-65 1.6937387177598566 1101 66-67 1.608579944183795 1101 68-69 1.5563931550561918 1101 70-71 1.5698200955692627 1101 72-73 1.5791372801101176 1101 74-75 1.3273473961485571 1101 76-77 1.3431200991847483 1101 78-79 1.2756839858662232 1101 80-81 1.2577625925649905 1101 82-83 1.1719174410248954 1101 84-85 0.9042030681371465 1101 86-87 1.029694056690726 1101 88-89 1.0044907024696883 1101 90-91 1.1038882131645416 1101 92-93 1.3508852022275022 1101 94-95 1.28369337772547 1101 96-97 1.2821335180692515 1101 98-99 1.2993422155496646 1101 100 1.443340262416271 1102 1 0.4915664300190947 1102 2 0.26332878437474605 1102 3 0.5686046509055878 1102 4 0.7574297710876294 1102 5 0.4077016403642375 1102 6 0.547966294539485 1102 7 -0.06839669369626478 1102 8 0.10447960175060444 1102 9 0.6142965127280675 1102 10-11 0.7879620777832059 1102 12-13 0.522143914185925 1102 14-15 0.6096019298761419 1102 16-17 0.4430905055358707 1102 18-19 0.4222574391449143 1102 20-21 0.3040865585849275 1102 22-23 0.18410137779223135 1102 24-25 0.3597711207157417 1102 26-27 0.08941448721452616 1102 28-29 0.4659837419754709 1102 30-31 0.4817795546170416 1102 32-33 0.6442096549062839 1102 34-35 0.7559278297204912 1102 36-37 0.8186168260656146 1102 38-39 0.7827828134785264 1102 40-41 0.6190573870076079 1102 42-43 0.909056258742126 1102 44-45 1.195826512183153 1102 46-47 1.0585316337086255 1102 48-49 0.8980929678292568 1102 50-51 1.1310348740134302 1102 52-53 1.1499595937198706 1102 54-55 1.1264760675695271 1102 56-57 0.9263476600116647 1102 58-59 1.201563110157025 1102 60-61 1.152235445570831 1102 62-63 0.8855073734443621 1102 64-65 0.9686230840873336 1102 66-67 1.0212533946925788 1102 68-69 1.2893718784604502 1102 70-71 1.3356387819707365 1102 72-73 1.37733339481872 1102 74-75 1.32896626756391 1102 76-77 1.331001690304081 1102 78-79 1.1573213586691828 1102 80-81 1.1805655527870051 1102 82-83 1.1998082828380312 1102 84-85 1.1938885445478782 1102 86-87 1.2454202361541888 1102 88-89 1.3679967154206594 1102 90-91 1.5141564832848005 1102 92-93 1.4737344159185284 1102 94-95 1.1036748763378625 1102 96-97 0.8874989204744352 1102 98-99 1.3144208464469784 1102 100 1.6244688470601183 1103 1 0.9851654963625727 1103 2 0.674371068242241 1103 3 0.8584511362274299 1103 4 0.611204795775663 1103 5 0.5853657035655999 1103 6 0.667025456044108 1103 7 0.6118259474743937 1103 8 0.8192116243738212 1103 9 0.9718869607036495 1103 10-11 0.8618196951635824 1103 12-13 0.7908574432280275 1103 14-15 1.2721389619256982 1103 16-17 1.0261769585388265 1103 18-19 1.0159462801539831 1103 20-21 1.1853595633429919 1103 22-23 1.2312600096263324 1103 24-25 1.3315818757206728 1103 26-27 1.3675812885431853 1103 28-29 1.12817963029579 1103 30-31 1.3431136061477034 1103 32-33 0.982408775114564 1103 34-35 0.9035263574159771 1103 36-37 0.7906340628014092 1103 38-39 0.733550386869311 1103 40-41 0.9204578717908021 1103 42-43 1.1610716999846034 1103 44-45 0.921142967879355 1103 46-47 0.9869632703007767 1103 48-49 0.8231760093949276 1103 50-51 0.9582455751535619 1103 52-53 1.0565939415069252 1103 54-55 1.2351662551985783 1103 56-57 1.498059662884451 1103 58-59 1.2327957163155645 1103 60-61 0.9072852705102363 1103 62-63 0.8476314420322026 1103 64-65 0.9428398001300664 1103 66-67 0.8244852826537965 1103 68-69 0.8713720580097828 1103 70-71 1.0088679766919881 1103 72-73 0.6859158528490639 1103 74-75 0.3852908927546821 1103 76-77 0.5127953881225586 1103 78-79 0.38985569747877236 1103 80-81 0.35861564705040294 1103 82-83 0.41696422143215983 1103 84-85 0.432078687289593 1103 86-87 0.42114516657882106 1103 88-89 0.6437036909121758 1103 90-91 0.51392306915427 1103 92-93 0.9420978237648328 1103 94-95 0.8489841500608222 1103 96-97 0.5773454057962724 1103 98-99 0.3671095976813845 1103 100 0.601953360930306 1104 1 0.2947712728927705 1104 2 0.5346009289487696 1104 3 0.44888596489116495 1104 4 0.3126153849040634 1104 5 0.3249098661276122 1104 6 0.424259359615597 1104 7 0.24719130594763783 1104 8 0.30859248343848833 1104 9 0.6121955601775468 1104 10-11 0.610173419366042 1104 12-13 0.4379188629974564 1104 14-15 0.5323741189618687 1104 16-17 0.5723079897342132 1104 18-19 0.18756501503737866 1104 20-21 0.35104640902559936 1104 22-23 0.8320226810950047 1104 24-25 0.5047012373364055 1104 26-27 0.05741057018434503 1104 28-29 0.27720069203333253 1104 30-31 0.7632537823389285 1104 32-33 0.6601359435201886 1104 34-35 0.6804537800454256 1104 36-37 1.0288100068721704 1104 38-39 0.8232172477347248 1104 40-41 1.080830733397704 1104 42-43 1.1515471338422714 1104 44-45 0.8016842527689292 1104 46-47 1.1195215740629365 1104 48-49 1.1051614268339733 1104 50-51 1.037302122299728 1104 52-53 0.9940795972808019 1104 54-55 1.0282298288087333 1104 56-57 0.859366443951842 1104 58-59 0.9204093667224953 1104 60-61 1.0032547636514906 1104 62-63 0.7016028065432707 1104 64-65 0.8254378263645492 1104 66-67 0.7565333733269668 1104 68-69 0.9603680504536847 1104 70-71 1.116436431752632 1104 72-73 1.135777265556726 1104 74-75 1.0243548547623433 1104 76-77 1.0304586474838189 1104 78-79 1.0452764908689538 1104 80-81 1.0901711790667186 1104 82-83 0.9882263367862194 1104 84-85 0.8420146017009067 1104 86-87 1.1755190521427892 1104 88-89 1.0068465329226584 1104 90-91 1.2082542309924484 1104 92-93 1.0553421787601565 1104 94-95 0.8077610510018047 1104 96-97 0.8037634980583377 1104 98-99 0.6720546041252504 1104 100 0.5003167950704466 1105 1 -0.16516555215229545 1105 2 -0.1566190753510952 1105 3 -0.22095047481975172 1105 4 -0.1512574713646444 1105 5 0.24943067913305583 1105 6 0.02999471939862275 1105 7 -0.3375077861191045 1105 8 0.05911756323408213 1105 9 -0.04285595552523347 1105 10-11 -0.11109603339330931 1105 12-13 -0.16494716309941992 1105 14-15 0.5411888443492714 1105 16-17 0.4499023800628237 1105 18-19 0.22596612638736957 1105 20-21 0.2161629188574068 1105 22-23 -0.04874699492663126 1105 24-25 0.12013610263483798 1105 26-27 0.5156191798391916 1105 28-29 0.23856804943489607 1105 30-31 0.05974608392492797 1105 32-33 0.10815717824326043 1105 34-35 0.08726245254447917 1105 36-37 0.23882538716224389 1105 38-39 0.13846358044513352 1105 40-41 0.06525198796631315 1105 42-43 -0.13818114769972567 1105 44-45 0.03811900230173393 1105 46-47 0.30890586632152406 1105 48-49 0.27043339336117356 1105 50-51 0.255305330661308 1105 52-53 0.007078423086618102 1105 54-55 0.17721191821286908 1105 56-57 0.2759986565636794 1105 58-59 0.33839769093239624 1105 60-61 0.23532349535638986 1105 62-63 0.2756679991276556 1105 64-65 0.1702209311985925 1105 66-67 0.27933093232608996 1105 68-69 0.3641006649376166 1105 70-71 0.3099386726443569 1105 72-73 0.1073981496753369 1105 74-75 0.2880193329074494 1105 76-77 0.5454916406867234 1105 78-79 0.4754863574077284 1105 80-81 0.5433357150946314 1105 82-83 0.4653957018944581 1105 84-85 0.4964955582557202 1105 86-87 0.6953067049120705 1105 88-89 0.31298872618510387 1105 90-91 0.4548763554570314 1105 92-93 0.2900156370101108 1105 94-95 0.3319147215989844 1105 96-97 0.3754141504803208 1105 98-99 0.3910418202927488 1105 100 0.43464461024235845 1106 1 0.06606737473280688 1106 2 -0.049396098562901614 1106 3 0.2995830457137707 1106 4 -0.010384243534893756 1106 5 -0.29393312510031677 1106 6 0.12172789808832363 1106 7 0.02254226958311989 1106 8 0.053120035497421725 1106 9 0.2590348681047274 1106 10-11 0.34414379141172446 1106 12-13 0.5152762562544311 1106 14-15 0.4773830848913434 1106 16-17 0.6141247616765071 1106 18-19 0.5861611604954433 1106 20-21 0.5196866571637528 1106 22-23 0.6440208717542788 1106 24-25 0.32243439188965084 1106 26-27 0.20372484620573061 1106 28-29 0.5346521063885206 1106 30-31 0.737123348258713 1106 32-33 0.8038169825987183 1106 34-35 0.5890174542994799 1106 36-37 0.9669450935428188 1106 38-39 0.6398599514067271 1106 40-41 0.8012310500323352 1106 42-43 0.9118821849723062 1106 44-45 0.68347129982795 1106 46-47 0.5545145467128094 1106 48-49 0.6391592620870412 1106 50-51 0.6989392525561016 1106 52-53 0.5301452505012705 1106 54-55 0.33348337883215606 1106 56-57 0.5033705795877736 1106 58-59 0.1796539669712871 1106 60-61 0.21154703679732023 1106 62-63 0.3016496555440895 1106 64-65 0.2839603058075326 1106 66-67 0.21774563161035587 1106 68-69 0.5585167071797486 1106 70-71 0.4057693989098681 1106 72-73 0.5958990729588649 1106 74-75 0.569071418307356 1106 76-77 0.47780198604054647 1106 78-79 0.5987408937057417 1106 80-81 0.6839418774399242 1106 82-83 0.6634819869372635 1106 84-85 0.6028181428391761 1106 86-87 0.8139982366470804 1106 88-89 0.6193891412811681 1106 90-91 0.557925480245288 1106 92-93 0.6263426472501621 1106 94-95 0.8417427314269972 1106 96-97 0.8196356164409195 1106 98-99 1.0107405704262789 1106 100 0.8135265978199975 1107 1 0.984956932405634 1107 2 0.7075808675395407 1107 3 0.9059074581218312 1107 4 1.015321527418024 1107 5 0.7973950308666247 1107 6 0.9131309252327924 1107 7 0.9619406576507146 1107 8 1.041669149521823 1107 9 0.9963691605720939 1107 10-11 0.9535878362169896 1107 12-13 0.8000583224053202 1107 14-15 0.9073124710944924 1107 16-17 1.0521431711778035 1107 18-19 1.2278071519513247 1107 20-21 0.6218197516922856 1107 22-23 0.7334258661022375 1107 24-25 1.0060376040099754 1107 26-27 1.1748040188058155 1107 28-29 1.261291564967138 1107 30-31 1.3863665621407009 1107 32-33 1.4223263121346648 1107 34-35 1.416505452890135 1107 36-37 1.39144264921908 1107 38-39 1.4145197279676367 1107 40-41 0.9500338452229613 1107 42-43 0.9591978330484068 1107 44-45 1.085940580624218 1107 46-47 0.8647528132248468 1107 48-49 0.7666311119923535 1107 50-51 0.8826892709068801 1107 52-53 0.994875053634118 1107 54-55 1.0566435297489605 1107 56-57 1.2169956658364285 1107 58-59 1.230573708005096 1107 60-61 1.098073160966031 1107 62-63 1.0312399193548814 1107 64-65 1.329653745037092 1107 66-67 1.2080343994656602 1107 68-69 1.238332318554292 1107 70-71 1.1807888507354036 1107 72-73 1.0897919337718776 1107 74-75 1.2448792195904446 1107 76-77 1.2024522201964842 1107 78-79 1.0301310019019319 1107 80-81 0.9914789093115601 1107 82-83 1.0421025955958996 1107 84-85 0.9327284442323709 1107 86-87 0.971866316568395 1107 88-89 1.0345204594543134 1107 90-91 1.0397288479802143 1107 92-93 0.9890568008388456 1107 94-95 1.1224435843711973 1107 96-97 0.8358283436602605 1107 98-99 0.7243876776095064 1107 100 0.5039122577874053 1108 1 0.49057458871370585 1108 2 0.5282304001162288 1108 3 0.6785710562807274 1108 4 0.6327777154165375 1108 5 0.97217132521466 1108 6 0.8294092636644237 1108 7 0.7485126008166105 1108 8 0.6799235307219718 1108 9 0.9022984324209204 1108 10-11 0.8652777072824875 1108 12-13 0.3440204139459624 1108 14-15 0.4491875961695371 1108 16-17 0.39221864503355164 1108 18-19 0.3157384821780269 1108 20-21 0.7725316073771253 1108 22-23 0.5130007538920509 1108 24-25 0.02310498729708854 1108 26-27 0.20631578300719866 1108 28-29 0.0452190643239021 1108 30-31 -0.006526316443590474 1108 32-33 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0.3566814102982576 2215 52-53 0.4565001768561956 2215 54-55 0.41619596154473726 2215 56-57 0.1767452529624478 2215 58-59 0.2300680173853351 2215 60-61 0.27799660324688347 2215 62-63 0.36269771659215877 2215 64-65 0.15741167925890664 2215 66-67 0.22948464334936602 2215 68-69 0.1764268250898624 2215 70-71 0.3700323631728324 2215 72-73 0.21287318993298499 2215 74-75 0.23070707994301998 2215 76-77 0.32641683465539373 2215 78-79 0.46348225844710456 2215 80-81 0.14566333916138774 2215 82-83 0.13059845405373238 2215 84-85 0.17867971870075294 2215 86-87 0.08734300999185507 2215 88-89 -0.01375699186496604 2215 90-91 0.025217447537254856 2215 92-93 0.39819891910643435 2215 94-95 -0.03508864540437884 2215 96-97 -0.28656798976269116 2215 98-99 -0.26603620635049907 2215 100 -0.14125935696595704 2216 1 -0.3147781432968273 2216 2 -0.2862833210096909 2216 3 0.08689330594821598 2216 4 -0.5955005615032007 2216 5 -0.6095039821874195 2216 6 -0.21175411409404887 2216 7 -0.13890902183207032 2216 8 -0.14381903869796986 2216 9 -0.661054896750386 2216 10-11 -0.6214626092127205 2216 12-13 -0.9638227499203822 2216 14-15 -1.0367994834428487 2216 16-17 -0.7770492901473602 2216 18-19 -0.6470950456849494 2216 20-21 -0.5240876483434036 2216 22-23 -0.520922049506332 2216 24-25 -0.6701850971197345 2216 26-27 -1.0245343567827092 2216 28-29 -0.7677746112006503 2216 30-31 -0.8905685519963669 2216 32-33 -1.0246819650338068 2216 34-35 -1.2437188405306756 2216 36-37 -1.1444525341002958 2216 38-39 -1.1685882595685584 2216 40-41 -0.9944075488553636 2216 42-43 -0.8426374197278079 2216 44-45 -0.6222240293330898 2216 46-47 -0.6822472279551022 2216 48-49 -0.9425629965305262 2216 50-51 -0.7768075101942671 2216 52-53 -0.6467221790737341 2216 54-55 -0.7581771316009167 2216 56-57 -0.6461771114761632 2216 58-59 -0.5495391250376969 2216 60-61 -0.6604613821425076 2216 62-63 -0.5188554808455201 2216 64-65 -0.4964433572676086 2216 66-67 -0.5177895425529826 2216 68-69 -0.5603938485539146 2216 70-71 -0.672802726593666 2216 72-73 -0.6197247509682065 2216 74-75 -0.7907894740445052 2216 76-77 -0.2726545044881483 2216 78-79 -0.5418721277737077 2216 80-81 -0.4343971939677367 2216 82-83 -0.5031335713750416 2216 84-85 -0.5282329701613939 2216 86-87 -0.7037386854219712 2216 88-89 -0.7540479884736513 2216 90-91 -0.6885933438908687 2216 92-93 -0.8582342452689673 2216 94-95 -0.6096671622211645 2216 96-97 -0.378327947948641 2216 98-99 -0.4067635763197437 2216 100 -0.29632384055029704 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7341.0 3 921.0 4 680.0 5 679.0 6 1375.0 7 2825.0 8 3142.0 9 1927.0 10 1179.0 11 1181.0 12 1129.0 13 693.0 14 598.0 15 568.0 16 579.0 17 530.0 18 474.0 19 385.0 20 363.0 21 356.0 22 464.0 23 885.0 24 1458.0 25 1471.0 26 1306.0 27 1059.0 28 1086.0 29 1413.0 30 1601.0 31 1994.0 32 2677.0 33 3575.0 34 5339.0 35 9318.0 36 15913.0 37 29546.0 38 45705.0 39 16947.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.97153974825905 21.3552726798192 14.176740654621382 21.496446917300368 2 16.495169411611204 24.849803989016266 36.12414226661052 22.530884332762007 3 19.18562234941724 29.736943562976364 28.53821287701296 22.539221210593436 4 12.984978999074537 21.842742222538618 33.350537481312735 31.82174129707411 5 12.692385280820211 41.96046089402048 31.364882343866814 13.982271481292496 6 27.556420418139965 40.00925508436365 16.706020551033486 15.728303946462896 7 23.598372035074814 38.99818455806448 20.03690212038872 17.36654128647199 8 22.63184752682047 39.25875818855027 19.530879141744993 18.57851514288427 9 22.85546450843455 23.46543406929207 19.39026778138406 34.28883364088932 10-11 21.42441774217475 33.084112149532714 25.154724818276218 20.336745290016317 12-13 21.803152713451993 32.26383577169678 25.06564444299392 20.867367071857302 14-15 20.50883413194084 33.54151522793693 22.901986381640434 23.047664258481806 16-17 19.858810268883385 35.58989722218103 23.750723004137807 20.80056950479778 18-19 20.336539317309345 33.81116536825304 25.69286633954159 20.159428974896016 20-21 22.66671413187615 32.26892601063814 24.838247465802798 20.22611239168291 22-23 21.79378820096264 32.40034757130105 24.902060219990094 20.903804007746214 24-25 21.458535298285558 32.59395631924248 24.823679038039078 21.12382934443288 26-27 21.645688766950826 32.161065136898245 25.162790283357534 21.0304558127934 28-29 20.80264800880297 32.66707992371552 25.26463775251439 21.265634314967123 30-31 21.602493941632037 32.958114950302104 24.83619418811937 20.60319691994649 32-33 21.154353816355766 32.69759536811008 25.31344452321136 20.834606292322796 34-35 21.10855174417398 32.606730048054835 24.903134384551308 21.38158382321988 36-37 20.87942067836618 32.585062659019876 25.24212536548624 21.29339129712771 38-39 20.943290452899248 32.628678378947555 25.330205048956067 21.097826119197126 40-41 20.66545444837641 32.47509069390104 25.38242718532998 21.477027672392566 42-43 20.542801210179746 32.80417630657887 25.676573530284152 20.976448952957227 44-45 20.069168231256803 32.9584249100546 25.51839097846946 21.45401588021913 46-47 20.12618165531111 32.65803110345175 25.566171214327877 21.64961602690926 48-49 20.11775698162457 32.0527385172112 25.198807599329648 22.630696901834575 50-51 20.09829219535055 31.567099104870717 25.248103263158516 23.086505436620218 52-53 20.192498776490826 30.754126562754898 25.669963962093462 23.383410698660814 54-55 20.34688526923944 30.863179623456276 25.41225250020761 23.377682607096677 56-57 20.66530434292595 30.57190904264627 25.355237284566496 23.407549329861286 58-59 20.48965670645807 30.173352116853263 25.3076295692148 24.029361607473867 60-61 21.04517051267983 29.842313954488432 24.950991876959215 24.16152365587252 62-63 20.69045288726755 29.816116499095415 24.990360945517097 24.503069668119938 64-65 21.262467339500148 29.840056230926415 25.111735240123256 23.785741189450178 66-67 21.602367902816333 29.55103566090113 24.903018008399176 23.94357842788336 68-69 21.76584859494328 30.00192778230889 24.73552309631497 23.49670052643286 70-71 21.701428533299328 29.658747505182397 25.347643379725326 23.292180581792948 72-73 21.62040332147094 29.727461447212338 25.172004744958482 23.480130486358245 74-75 21.985794569907622 29.493023324782396 25.2791411497798 23.242040955530182 76-77 22.240509594939635 28.84472241250975 25.734188589272527 23.180579403278088 78-79 22.57787108865324 28.656323393352785 26.174590183379593 22.591215334614382 80-81 23.05983869054679 28.338571936899537 26.210117423793143 22.39147194876053 82-83 22.646869560318013 28.047778756364522 26.458909735751924 22.846441947565545 84-85 22.913108454312553 28.08704573488946 26.28421814213872 22.715627668659266 86-87 23.28130652133949 27.736003463182925 26.261185665743547 22.721504349734033 88-89 23.314820800061675 27.736479574439066 26.106526631657918 22.842172993841345 90-91 23.31010762890266 27.6884988288315 26.287840602484657 22.713552939781184 92-93 23.46951785401897 27.897516181733543 25.601385269063016 23.03158069518447 94-95 22.772045306291883 27.96507145822214 25.913835023424063 23.34904821206191 96-97 22.18390566020952 28.663094627411795 25.993850241216432 23.15914947116226 98-99 22.073139271809683 29.47276032107031 25.414014060910844 23.040086346209158 100 21.43323132398643 29.990747989466943 25.257395677650464 23.318625008896163 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1318.0 1 836.5 2 322.5 3 256.5 4 179.0 5 119.0 6 111.0 7 118.5 8 116.0 9 112.5 10 99.0 11 89.5 12 92.5 13 86.0 14 92.5 15 107.5 16 101.5 17 111.0 18 131.0 19 148.5 20 189.5 21 235.5 22 330.0 23 472.5 24 669.5 25 896.5 26 1158.0 27 1475.5 28 1875.0 29 2464.0 30 2950.0 31 3367.5 32 3776.0 33 4080.5 34 4445.5 35 4699.0 36 4873.5 37 5172.0 38 5286.0 39 4990.5 40 4807.5 41 4775.0 42 5044.5 43 5456.0 44 5332.5 45 5077.0 46 5395.0 47 5775.5 48 5658.5 49 5447.0 50 5539.0 51 5638.5 52 5459.0 53 5203.5 54 4870.0 55 4550.0 56 3891.0 57 3139.5 58 2815.5 59 2542.5 60 2285.0 61 2051.5 62 1738.5 63 1478.0 64 1235.5 65 1041.0 66 902.0 67 803.0 68 722.5 69 594.0 70 439.5 71 319.0 72 247.5 73 223.5 74 183.0 75 142.5 76 202.0 77 178.0 78 65.0 79 34.5 80 27.5 81 16.0 82 14.5 83 11.5 84 6.5 85 3.5 86 1.0 87 0.5 88 0.5 89 1.5 90 2.0 91 1.0 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.061059108774134646 2 0.04505332297897314 3 0.055723846842414146 4 0.07410086016278478 5 0.08714261155143489 6 0.078843315213203 7 0.08002892897580756 8 0.09544190788966678 9 0.09247787348315539 10-11 0.09722032853357362 12-13 0.09929515261813159 14-15 0.0987023457368293 16-17 0.0720260360782268 18-19 0.08892103219534173 20-21 0.08566059434817919 22-23 0.05364902275785617 24-25 0.01985903052362632 26-27 0.17636004718742773 28-29 0.06491235350259945 30-31 0.07143322919692452 32-33 0.07024761543431997 34-35 0.01630218923581265 36-37 0.046238936741577696 38-39 0.07084042231562224 40-41 0.07558287736604047 42-43 0.07054401887497111 44-45 0.06787638790911085 46-47 0.07261884295952908 48-49 0.0720260360782268 50-51 0.0652087569432506 52-53 0.0690620016717154 54-55 0.061059108774134646 56-57 0.06194831909608807 58-59 0.06016989845218123 60-61 0.05602025028306529 62-63 0.06165191565543692 64-65 0.0583914778082744 66-67 0.059873495011530095 68-69 0.06016989845218123 70-71 0.053056215876553896 72-73 0.052759812435902755 74-75 0.05424182963915845 76-77 0.050092181470042504 78-79 0.04564612986027542 80-81 0.04090367480985719 82-83 0.04653534018222883 84-85 0.03853244728464808 86-87 0.03438279911553213 88-89 0.038236043843996946 90-91 0.03201157159032302 92-93 0.034086395674881 94-95 0.03497560599683441 96-97 0.038236043843996946 98-99 0.03882885072529922 100 0.04564612986027542 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 168689.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.351896092809845 #Duplication Level Percentage of deduplicated Percentage of total 1 78.14372294372295 40.12828340911381 2 8.779220779220779 9.01659266460765 3 3.7240981240981244 5.737184997243448 4 2.2152958152958155 4.550385620876287 5 1.4741702741702742 3.7850719371150463 6 1.0620490620490621 3.2722939847885755 7 0.8300144300144301 2.983597033594366 8 0.6441558441558441 2.6462899181333697 9 0.4675324675324675 2.1607810823468037 >10 2.6031746031746033 22.644630058865722 >50 0.049639249639249636 1.6023570001600578 >100 0.005772005772005772 0.3491632530870419 >500 0.0 0.0 >1k 0.0011544011544011544 1.1233690400678171 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1895 1.1233690400678171 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.928068813022782E-4 0.0 14-15 0.0 0.0 0.0 5.928068813022782E-4 0.0 16-17 0.0 0.0 0.0 8.892103219534174E-4 0.0 18-19 0.0 0.0 0.0 0.00326043784716253 0.0 20-21 0.0 0.0 0.0 0.0041496481691159475 0.0 22-23 0.0 0.0 0.0 0.007706489456929616 0.0 24-25 0.0 0.0 0.0 0.015412978913859232 0.0 26-27 0.0 0.0 0.0 0.03438279911553213 0.0 28-29 0.0 0.0 0.0 0.07113682575627338 0.0 30-31 0.0 0.0 0.0 0.14820172032556955 0.0 32-33 0.0 0.0 0.0 0.26883792067058315 0.0 34-35 0.0 0.0 0.0 0.46950304999140424 0.0 36-37 0.0 0.0 0.0 0.733302112170918 0.0 38-39 0.0 0.0 0.0 1.0205170461618718 0.0 40-41 0.0 0.0 0.0 1.3329262726081725 0.0 42-43 0.0 0.0 0.0 1.596428931347035 0.0 44-45 0.0 0.0 0.0 1.8723805345932454 0.0 46-47 0.0 0.0 0.0 2.22480422552745 0.0 48-49 0.0 0.0 0.0 2.5523300274469585 0.0 50-51 0.0 0.0 0.0 2.8285780341338205 0.0 52-53 0.0 0.0 0.0 3.1012691995328683 0.0 54-55 0.0 0.0 0.0 3.4379835081125623 0.0 56-57 0.0 0.0 0.0 3.810266229570393 0.0 58-59 0.0 0.0 0.0 4.1961835092981765 0.0 60-61 0.0 0.0 0.0 4.56935544107796 0.0 62-63 0.0 0.0 0.0 4.92592878018128 0.0 64-65 0.0 0.0 0.0 5.363420258582361 0.0 66-67 0.0 0.0 0.0 5.817213926219256 0.0 68-69 0.0 0.0 0.0 6.352814943475863 0.0 70-71 0.0 0.0 0.0 6.9308016527455845 0.0 72-73 0.0 0.0 0.0 7.482112052356704 0.0 74-75 0.0 0.0 0.0 8.041425344865402 0.0 76-77 0.0 0.0 0.0 8.7038870347207 0.0 78-79 0.0 0.0 0.0 9.37286960027032 0.0 80-81 0.0 0.0 0.0 10.047187427751663 0.0 82-83 0.0 0.0 0.0 10.744921127044442 0.0 84-85 0.0 0.0 0.0 11.489486569960103 0.0 86-87 0.0 0.0 0.0 12.248575781467672 0.0 88 0.0 0.0 0.0 12.892364054561945 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATAGA 30 1.3672705E-4 62.671413 4 GAACATA 50 2.9970784E-5 47.00356 3 ACTGGTC 55 5.2339004E-5 42.76858 8 CGCAGAA 60 8.788127E-5 39.169636 2 GCAGTGT 50 0.0016972771 37.602848 3 GGAATCA 165 0.0 37.033108 1 ACAACAC 290 0.0 35.657875 5 CTACAGA 55 0.0027094567 34.184406 1 CCTAATT 35 2.1807691E-4 33.57397 40-41 TGTAGAG 70 2.1761612E-4 33.57397 2 GGAAAAC 60 0.004149464 31.335707 7 GAATCAA 200 0.0 30.552315 2 CTACACT 80 4.7616728E-4 29.377226 4 AGAACAA 145 2.6298949E-8 29.174623 2 CCATGCA 65 0.0061105466 28.95104 9 GAGAACA 260 0.0 28.925268 1 CAACACA 570 0.0 28.03721 6 AACAACA 440 0.0 27.774832 4 GAACAAA 290 0.0 27.553812 1 AACGGTA 35 0.0088240625 26.859179 18-19 >>END_MODULE