FastQCFastQC Report
Wed 25 May 2016
SRR1033016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033016_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168689
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19141.1346323708125603No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17101.0136997670268957No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT15480.9176650522559266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9230.5471607514420027No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT7210.4274137614189426RNA PCR Primer, Index 33 (95% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5190.30766677139588233No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT4950.29343940624462767RNA PCR Primer, Index 33 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.1766564506280789No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.17250680245896294No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.16480031300203335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2620.1553154029011969No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1860.11026207992222373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1820.10789085239701462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGTC657.3123374E-1057.8408628
CTAGCAC250.005224075656.4115643
GGTATCA9750.056.1625981
TTCGTGG352.9316387E-453.709378
GTACCCA451.5669897E-552.45071
TCTACAC752.59206E-950.1436123
CTACACT751.4685793E-743.875664
CTCTACA1006.7484507E-1042.447142
TACACTG802.443703E-741.1212355
GTATCAA15900.038.892691
TCCGCAG250.001701087537.5965692-93
TACCCAT651.3798861E-436.279612
ACTATAC550.002711685834.178698
CTGGTCC1457.348717E-1032.4108289
CTATACA753.1812428E-431.470421
TGTATAG600.00414684831.3397583
CTAACCA600.00414684831.3397584
ATATAGT753.2598697E-431.3397584
GTATAGC600.00414684831.3397584
TATCAAC20500.029.9085562
Produced by FastQC (version 0.11.5)