##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033016_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	168689
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.531807053216276	33.0	31.0	34.0	31.0	34.0
2	32.506808387031754	34.0	31.0	34.0	31.0	34.0
3	32.55515771627077	34.0	31.0	34.0	30.0	34.0
4	36.14217287434273	37.0	35.0	37.0	35.0	37.0
5	36.14744885558632	37.0	35.0	37.0	35.0	37.0
6	36.2474968729437	37.0	37.0	37.0	35.0	37.0
7	36.1900953826272	37.0	37.0	37.0	35.0	37.0
8	36.21884651637036	37.0	37.0	37.0	35.0	37.0
9	37.958361244657326	39.0	38.0	39.0	35.0	39.0
10-11	37.934720106231	39.0	38.0	39.0	35.0	39.0
12-13	37.90572888570091	39.0	38.0	39.0	35.0	39.0
14-15	39.25080473534137	41.0	39.0	41.0	36.0	41.0
16-17	39.246821073099014	41.0	39.0	41.0	36.0	41.0
18-19	39.35021252126695	41.0	39.0	41.0	36.0	41.0
20-21	39.370978546318966	41.0	39.0	41.0	36.0	41.0
22-23	39.239956369413534	41.0	39.0	41.0	35.5	41.0
24-25	39.150157983033864	41.0	39.0	41.0	35.0	41.0
26-27	38.92366425789471	41.0	38.5	41.0	35.0	41.0
28-29	38.776022740071966	40.0	38.0	41.0	35.0	41.0
30-31	38.507315236915275	40.0	38.0	41.0	35.0	41.0
32-33	38.14930137709038	40.0	37.0	41.0	34.5	41.0
34-35	37.82266478549283	40.0	36.0	41.0	33.5	41.0
36-37	37.54194405088654	40.0	36.0	41.0	33.0	41.0
38-39	37.230649301377085	40.0	35.5	41.0	33.0	41.0
40-41	36.97298875445346	40.0	35.0	41.0	33.0	41.0
42-43	36.63024559989093	40.0	35.0	41.0	31.5	41.0
44-45	36.099653800781326	40.0	35.0	41.0	29.5	41.0
46-47	35.32430686055404	40.0	35.0	41.0	23.0	41.0
48-49	34.59710176715731	40.0	35.0	41.0	5.5	41.0
50-51	34.188079839230774	40.0	35.0	41.0	2.0	41.0
52-53	33.971622334592055	39.5	35.0	41.0	2.0	41.0
54-55	33.77423839135925	39.0	35.0	41.0	2.0	41.0
56-57	33.57172370456877	39.0	35.0	41.0	2.0	41.0
58-59	33.340783927819835	39.0	35.0	41.0	2.0	41.0
60-61	33.10487939344	38.0	34.0	41.0	2.0	41.0
62-63	32.80833960720616	37.5	34.0	41.0	2.0	41.0
64-65	32.46753196711107	37.0	34.0	40.0	2.0	41.0
66-67	32.04555424479368	36.5	33.0	40.0	2.0	41.0
68-69	31.62257764288128	36.0	33.0	39.0	2.0	41.0
70-71	31.107167628001825	35.5	33.0	39.0	2.0	41.0
72-73	30.69798860625174	35.0	32.5	39.0	2.0	41.0
74-75	30.38755935478899	35.0	32.0	37.5	2.0	40.0
76-77	30.06690122058937	35.0	32.0	37.0	2.0	39.0
78-79	29.748869220873914	35.0	31.5	36.5	2.0	39.0
80-81	29.4752354925336	35.0	31.0	36.0	2.0	39.0
82-83	29.21764311840132	35.0	31.0	36.0	2.0	37.0
84-85	29.0292698397643	35.0	31.0	35.0	2.0	37.0
86-87	28.863017149903076	35.0	31.0	35.0	2.0	36.5
88-89	28.675607182448175	35.0	31.0	35.0	2.0	36.0
90-91	28.4964935472971	35.0	31.0	35.0	2.0	36.0
92-93	28.34292692469574	35.0	31.0	35.0	2.0	36.0
94-95	28.21566314341777	35.0	31.0	35.0	2.0	35.5
96-97	28.07028911191601	35.0	30.0	35.0	2.0	35.0
98-99	27.936059849782737	35.0	30.0	35.0	2.0	35.0
100	27.82093082536502	35.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15290553689492015
1101	2	0.14429181570930183
1101	3	0.07899143963715716
1101	4	0.013028120029005663
1101	5	0.05678369599823441
1101	6	0.20421663808458135
1101	7	0.12046897697832293
1101	8	0.18560596386105033
1101	9	0.03588547221623628
1101	10-11	0.2257118419872981
1101	12-13	0.36661014644306533
1101	14-15	0.6172974970859144
1101	16-17	0.5770928993599185
1101	18-19	0.2899872450090726
1101	20-21	0.34917474196127785
1101	22-23	0.5444744797317753
1101	24-25	0.4260393108510456
1101	26-27	0.5385297883612665
1101	28-29	0.2818683727503952
1101	30-31	0.46633954527650445
1101	32-33	0.609800547309419
1101	34-35	0.7306107164289699
1101	36-37	0.6531842422097753
1101	38-39	0.47132983520482696
1101	40-41	0.4868085661808408
1101	42-43	0.11008707324551636
1101	44-45	0.08175570895415518
1101	46-47	0.22475819705444877
1101	48-49	0.5139998516041899
1101	50-51	0.7728431052825755
1101	52-53	1.2048717706132237
1101	54-55	1.3746440748177307
1101	56-57	1.2659040412880458
1101	58-59	1.1254847019567151
1101	60-61	1.107775665317341
1101	62-63	1.368097069170247
1101	64-65	1.2840682064935578
1101	66-67	1.3490914915142938
1101	68-69	1.0545406816394909
1101	70-71	1.1575313444402724
1101	72-73	1.241473029894287
1101	74-75	1.4377357966628068
1101	76-77	1.4197835026684693
1101	78-79	1.4004110247115733
1101	80-81	1.1910769582344969
1101	82-83	1.1641379784521853
1101	84-85	0.9760808492972863
1101	86-87	1.065164823601652
1101	88-89	1.2930868082876295
1101	90-91	1.4631105876843051
1101	92-93	1.0180550044343946
1101	94-95	0.9797154328337925
1101	96-97	1.0747077550318487
1101	98-99	1.0660212995686429
1101	100	1.2060452965750876
1102	1	0.09518120757022075
1102	2	0.13328349008487805
1102	3	0.16271021854557688
1102	4	-0.10575078838912333
1102	5	-0.004085869219153437
1102	6	0.0708216334592322
1102	7	-0.10802315993194611
1102	8	0.14231271686752933
1102	9	0.1499465267953326
1102	10-11	0.10457400664964922
1102	12-13	0.13862679769191288
1102	14-15	0.22118278848276418
1102	16-17	0.2562788383053345
1102	18-19	0.19872915065199237
1102	20-21	0.1308121147642396
1102	22-23	0.13309612635526946
1102	24-25	0.06614106848287093
1102	26-27	0.2788165598691279
1102	28-29	0.2414428408354965
1102	30-31	0.08673732510999344
1102	32-33	0.0949069302881469
1102	34-35	0.32686418543914186
1102	36-37	0.466135213532624
1102	38-39	0.33557590365995793
1102	40-41	0.7087789639606754
1102	42-43	0.5868215783333994
1102	44-45	0.84655681903741
1102	46-47	0.9703178640294752
1102	48-49	1.1500775574321196
1102	50-51	0.8021215511660174
1102	52-53	0.819210346006372
1102	54-55	0.6620168777779512
1102	56-57	0.4283462244517864
1102	58-59	0.1814051459900199
1102	60-61	0.3815036949195658
1102	62-63	0.7166632116309302
1102	64-65	0.9791190853094633
1102	66-67	0.811163646925948
1102	68-69	0.5397858250252447
1102	70-71	0.5712223712672824
1102	72-73	0.3682075349821652
1102	74-75	0.5335267309828815
1102	76-77	0.22459386344552357
1102	78-79	0.27839437346273144
1102	80-81	0.3470899092058204
1102	82-83	0.2582175344188826
1102	84-85	0.23177002598553642
1102	86-87	0.44610839436946037
1102	88-89	0.6498398147631157
1102	90-91	0.5176896811163125
1102	92-93	0.5428006103548384
1102	94-95	0.6223148777921672
1102	96-97	0.6616180233019691
1102	98-99	0.34738115155753846
1102	100	0.22325158704687098
1103	1	0.33437682656654033
1103	2	0.28344059574965996
1103	3	0.2745335420036952
1103	4	0.2354827155160777
1103	5	0.20519683120278387
1103	6	0.13549530254981335
1103	7	0.19484065190035693
1103	8	0.1263507813257405
1103	9	0.31321073363616136
1103	10-11	0.3718960283751116
1103	12-13	0.4480172277125618
1103	14-15	0.4678386821895586
1103	16-17	0.6587476416090468
1103	18-19	0.4025445739395366
1103	20-21	0.4025869599029406
1103	22-23	0.3531517866479348
1103	24-25	0.4268556748209633
1103	26-27	0.2176620576696493
1103	28-29	0.38440001832001514
1103	30-31	0.4482419485052702
1103	32-33	0.39840814224613297
1103	34-35	0.7178148991477329
1103	36-37	0.5082933964933858
1103	38-39	0.2191650947902204
1103	40-41	0.42659025761359715
1103	42-43	0.13728391786118976
1103	44-45	0.12909564658027506
1103	46-47	0.16099925374129498
1103	48-49	0.2062764980910714
1103	50-51	0.2594785850110668
1103	52-53	0.49144304193958277
1103	54-55	0.5334864890536508
1103	56-57	0.6137668187600696
1103	58-59	0.5356021116063161
1103	60-61	0.6198284996596612
1103	62-63	0.45450323317685104
1103	64-65	0.4649616205460205
1103	66-67	0.3293519941743597
1103	68-69	0.6986151633859556
1103	70-71	0.7934146686009775
1103	72-73	0.7663491994044662
1103	74-75	0.7896987392421515
1103	76-77	0.7011447207935824
1103	78-79	0.9998056312057955
1103	80-81	0.8274831222411052
1103	82-83	0.7732188810388685
1103	84-85	0.5398856557548157
1103	86-87	0.4901877551738423
1103	88-89	0.4005625008211098
1103	90-91	0.6760073038831678
1103	92-93	0.5186512253112951
1103	94-95	0.4527754218266402
1103	96-97	0.5459882540228591
1103	98-99	0.6004828662536532
1103	100	0.653559514149844
1104	1	0.28505034315287503
1104	2	0.38087540675896037
1104	3	0.3761171445671181
1104	4	0.22372486370738187
1104	5	0.11808986299841706
1104	6	0.270216274249762
1104	7	0.3210874961706054
1104	8	0.2947564095587083
1104	9	0.2770897654671032
1104	10-11	0.3000160078959695
1104	12-13	0.3128990312893478
1104	14-15	0.41957146470461026
1104	16-17	0.4645224063637414
1104	18-19	0.5088066010214405
1104	20-21	0.4689764519849291
1104	22-23	0.517223251789261
1104	24-25	0.5890645973709709
1104	26-27	0.4692465429546857
1104	28-29	0.6693160714951674
1104	30-31	0.8267088376177796
1104	32-33	1.0462022209495885
1104	34-35	1.204732964928148
1104	36-37	1.5476539529610918
1104	38-39	1.4310624166128605
1104	40-41	1.61146747103804
1104	42-43	1.513261532041227
1104	44-45	1.900629640189372
1104	46-47	1.7773596160102443
1104	48-49	1.6778528623373177
1104	50-51	1.8870053756118494
1104	52-53	1.7486592910789298
1104	54-55	1.6895340147849822
1104	56-57	1.8149125914062907
1104	58-59	1.6955865757060309
1104	60-61	1.7432859436509744
1104	62-63	1.7657685263286993
1104	64-65	1.4468570003811294
1104	66-67	1.5125079004646267
1104	68-69	1.722987106962389
1104	70-71	1.9909677643056511
1104	72-73	1.961174685342705
1104	74-75	1.874219333137244
1104	76-77	1.8161633462194935
1104	78-79	1.8514024745933284
1104	80-81	1.9943969509578032
1104	82-83	1.810981711397428
1104	84-85	1.9516311494610115
1104	86-87	2.1297636957137165
1104	88-89	2.3395576105434053
1104	90-91	2.0555246317083196
1104	92-93	2.2333997379253887
1104	94-95	2.2044834881366846
1104	96-97	2.2649296942714336
1104	98-99	2.209181204971589
1104	100	2.18126814540172
1105	1	0.171087355076736
1105	2	-0.07112320405354922
1105	3	-0.0012457145130397862
1105	4	0.004332467855093114
1105	5	0.08445168018796068
1105	6	0.17338659855888494
1105	7	0.1054492141324701
1105	8	0.06307631959227677
1105	9	0.1706680809118879
1105	10-11	0.14685808704658854
1105	12-13	0.1423018460478147
1105	14-15	0.21571646941792721
1105	16-17	0.15733005351011542
1105	18-19	0.07516511062171816
1105	20-21	0.09403640204033081
1105	22-23	0.18597645601635548
1105	24-25	0.04007093140440787
1105	26-27	0.10117800970514423
1105	28-29	0.09293556642628431
1105	30-31	0.16811820140298295
1105	32-33	0.15367406509598425
1105	34-35	0.27902968876097844
1105	36-37	-0.14286318862026803
1105	38-39	-0.009500204320872285
1105	40-41	0.058942953532614695
1105	42-43	0.17826889142733648
1105	44-45	0.04282290263004285
1105	46-47	0.16685305871452982
1105	48-49	-0.30497248041162095
1105	50-51	-0.4210304125039812
1105	52-53	-0.05678830843815774
1105	54-55	-0.19037568802811933
1105	56-57	-0.23172441720997483
1105	58-59	-0.1683888158298501
1105	60-61	-0.4333310540502495
1105	62-63	-0.36510451185742454
1105	64-65	-0.33786855239972624
1105	66-67	-0.46829981283353206
1105	68-69	-0.45316682824193677
1105	70-71	-0.47646075042138847
1105	72-73	-0.45496965785275023
1105	74-75	0.025285203777432486
1105	76-77	-0.2655919914026761
1105	78-79	-0.45571545750186715
1105	80-81	-0.16208509710146757
1105	82-83	0.04477038951938184
1105	84-85	-0.21403772777781427
1105	86-87	-0.09333320011376145
1105	88-89	0.09960854741806457
1105	90-91	-0.1001305190350621
1105	92-93	-0.08767622086205051
1105	94-95	-0.3406402983915022
1105	96-97	0.0839962925812543
1105	98-99	0.2776813786200272
1105	100	0.17916782621935567
1106	1	0.22750684627883544
1106	2	0.22998931445027893
1106	3	0.3326929672517309
1106	4	0.15860475256216233
1106	5	0.10093343580015102
1106	6	0.10530779134964519
1106	7	-0.06422130336413545
1106	8	-0.14492954058749774
1106	9	-0.08432604364745089
1106	10-11	0.12296717185132167
1106	12-13	-0.07988137613541113
1106	14-15	0.060910053741949355
1106	16-17	0.17566600662868126
1106	18-19	0.32865603802568444
1106	20-21	0.20968338725253233
1106	22-23	0.23092739227164572
1106	24-25	0.28186439058917045
1106	26-27	0.190309207531989
1106	28-29	0.11623130711333118
1106	30-31	0.06403133644655412
1106	32-33	0.25381599132486343
1106	34-35	0.30510291048637583
1106	36-37	0.4116868441906405
1106	38-39	0.5611653228664011
1106	40-41	0.4499191923349457
1106	42-43	0.39575939152653206
1106	44-45	0.5359020913614074
1106	46-47	0.500425479243475
1106	48-49	0.4138991297643386
1106	50-51	0.6195864324606859
1106	52-53	0.6274334627401288
1106	54-55	0.7448723776547226
1106	56-57	0.32732757662462575
1106	58-59	0.5263324441088102
1106	60-61	0.5602517438803787
1106	62-63	0.7472308613475178
1106	64-65	0.59779155717343
1106	66-67	0.9626217920011158
1106	68-69	0.8456184053259044
1106	70-71	0.8952180995183312
1106	72-73	0.5933615643915147
1106	74-75	0.539523609320181
1106	76-77	0.7354457487654962
1106	78-79	0.6787558291433342
1106	80-81	0.4850756152766351
1106	82-83	0.656533479543338
1106	84-85	0.8139354741084297
1106	86-87	0.7486632288391881
1106	88-89	0.7832580354153791
1106	90-91	0.3796793303400072
1106	92-93	0.45716025875703536
1106	94-95	0.7587233227112478
1106	96-97	0.7857451899823253
1106	98-99	0.7520965052090638
1106	100	0.7861695201775731
1107	1	0.025413389432529243
1107	2	0.02005640489759486
1107	3	0.07917328787653588
1107	4	0.012928930080256862
1107	5	0.04306241975422864
1107	6	0.11107727620658636
1107	7	0.13875299086491566
1107	8	0.1652720243669208
1107	9	0.04618469522830537
1107	10-11	0.1748191324485262
1107	12-13	0.3289592950626741
1107	14-15	0.28355638285215434
1107	16-17	0.41175978644869105
1107	18-19	0.245343502241667
1107	20-21	0.16597917244565963
1107	22-23	0.35037628168250734
1107	24-25	0.42679150129575305
1107	26-27	0.3970270606376758
1107	28-29	0.3700813005070458
1107	30-31	0.12085268794471205
1107	32-33	0.1520058705033378
1107	34-35	0.16783174469809836
1107	36-37	0.19762133925060255
1107	38-39	0.6216935316835759
1107	40-41	0.23994958122464993
1107	42-43	0.23485976294629296
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2216	74-75	-1.6561191711601388
2216	76-77	-1.4295808043476974
2216	78-79	-1.2253463132259554
2216	80-81	-1.227216873189139
2216	82-83	-1.1310904001383015
2216	84-85	-0.9866722506870182
2216	86-87	-1.1084656896615712
2216	88-89	-1.2767060028880124
2216	90-91	-1.2821413350907669
2216	92-93	-1.0932697483516698
2216	94-95	-1.165764083725616
2216	96-97	-1.500798444936759
2216	98-99	-1.5093436352924492
2216	100	-1.5469919032365596
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	1.0
4	1.0
5	11.0
6	42.0
7	105.0
8	158.0
9	211.0
10	337.0
11	632.0
12	972.0
13	1356.0
14	1414.0
15	1937.0
16	2580.0
17	3839.0
18	4454.0
19	2022.0
20	921.0
21	802.0
22	820.0
23	886.0
24	1008.0
25	1486.0
26	1609.0
27	1974.0
28	1267.0
29	1558.0
30	1935.0
31	2315.0
32	2950.0
33	3768.0
34	5206.0
35	8637.0
36	15491.0
37	29540.0
38	47677.0
39	18751.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.725202972381524	19.951488266446844	14.68517782693528	21.638130934236354
2	16.555917455185202	21.792235271741713	39.13389757976321	22.517949693309877
3	18.683898506046955	27.165639079914634	32.981977709271995	21.16848470476642
4	12.478954731924782	19.945578715230848	35.743757558511774	31.831708994332597
5	11.684885706155743	37.31385753580575	35.380110025609405	15.6211467324291
6	27.420875101518178	38.09377019248439	17.07461660214952	17.41073810384791
7	22.176312622636924	36.324834458678396	22.239150152054965	19.259702766629715
8	24.53642227070094	33.53884093711467	21.377928483353884	20.546808308830503
9	25.20733420673547	15.190676333370877	21.65760660149743	37.94438285839622
10-11	23.338056056151004	26.618965190173576	27.785319643365263	22.257659110310158
12-13	25.277581822169793	25.256833581324212	29.079252351961298	20.386332244544693
14-15	22.37467284373268	25.693215601537744	28.644217003530176	23.2878945511994
16-17	19.13877016284405	29.070656652182418	29.720965800971015	22.069607384002513
18-19	19.009354548041355	24.601038603812956	35.70674855354263	20.682858294603054
20-21	20.75535452815536	23.16452169376782	36.029320228349206	20.050803549727604
22-23	19.662218639033963	24.33531528433982	36.17544712459022	19.827018952035996
24-25	19.70347711906858	24.92701043639608	36.200452312991104	19.169060131544235
26-27	19.989744440953473	24.595261101790868	36.47777863405439	18.937215823201274
28-29	19.2441074646685	24.467952670018022	36.60129944038699	19.68664042492649
30-31	20.761282596968385	24.183260319285786	35.95610857850838	19.099348505237447
32-33	19.998340140732353	24.06707017055054	36.56195721119931	19.372632477517797
34-35	19.764774229499256	23.59045343798351	36.22642851638222	20.418343816135017
36-37	19.62279698142736	24.17110777821909	36.141657132355995	20.064438107997557
38-39	19.86406938211739	23.366372436851247	36.22257527165375	20.54698290937761
40-41	19.845692368797017	23.16244686968326	36.12535494312018	20.86650581839954
42-43	20.594111056441143	23.604680803134762	35.491347983567394	20.3098601568567
44-45	20.048432322202398	24.198673298199648	34.80428480813805	20.948609571459905
46-47	20.79062653759285	23.730652265411496	33.60948253887331	21.869238658122345
48-49	21.262797218550116	24.112123493529513	31.781562520377737	22.84351676754264
50-51	20.76780347266271	24.298264854258427	31.780376906615132	23.15355476646373
52-53	20.6216765764217	24.442316926414883	32.2288353123203	22.707171184843116
54-55	20.65339174457137	24.918933658981913	31.398905678497112	23.0287689179496
56-57	20.81463521628559	25.03156696642935	30.68398057964657	23.469817237638495
58-59	19.75262168843256	25.368577678449693	31.34347823509535	23.535322398022394
60-61	20.514082127465336	26.175387843902094	29.963423815423653	23.347106213208924
62-63	19.645916449798147	26.787164545406046	30.062126161160478	23.50479284363533
64-65	19.702825910403167	26.707135616430232	30.317625927001764	23.272412546164837
66-67	19.816348428172553	26.6460765076561	29.8460480529258	23.691527011245547
68-69	20.103326545674424	26.658604469184326	29.69348829351141	23.54458069162984
70-71	20.267178061402937	26.15730723402237	29.830931477452587	23.744583227122103
72-73	20.114293505484962	26.09128660222837	29.468221010916572	24.326198881370097
74-75	20.683328492077134	25.849936866067143	29.11778479924595	24.348949842609773
76-77	20.69192419185602	25.783246091920635	29.092886910231254	24.43194280599209
78-79	20.725417780649597	25.712405669605015	29.256501609470682	24.305674940274706
80-81	20.82026688165796	25.964644997599134	29.17469425985097	24.040393860891935
82-83	20.36973365186823	25.90862474731607	29.2224152137958	24.499226387019903
84-85	20.75535452815536	26.128852503719862	28.69363147567417	24.422161492450602
86-87	21.08590686383482	26.148196231515485	28.37804592488522	24.387850979764476
88-89	20.941930397099927	26.311093721748463	28.158614700137235	24.588361181014374
90-91	20.800246612779404	26.211203689706586	28.473994504566193	24.514555192947824
92-93	20.951635707235525	26.225557758827662	27.81695254863254	25.00585398530427
94-95	20.81416579721142	25.925969837807077	27.97827942710803	25.28158493787347
96-97	20.727196201293506	26.00021341047727	28.064070567731147	25.208519820498076
98-99	20.940606678562325	26.312029830042267	27.492901137596405	25.254462353799
100	20.66880472348523	26.152861182412607	27.506239292425704	25.672094801676458
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	81.0
1	58.5
2	123.0
3	201.0
4	115.5
5	68.0
6	162.5
7	312.0
8	485.0
9	579.0
10	412.0
11	195.5
12	153.5
13	149.5
14	155.0
15	173.5
16	182.5
17	202.5
18	229.0
19	264.0
20	292.5
21	362.5
22	454.0
23	551.5
24	750.0
25	991.0
26	1304.5
27	1622.5
28	2042.5
29	2652.0
30	3116.5
31	3610.5
32	4032.0
33	4330.5
34	4675.5
35	4847.5
36	4947.0
37	5136.5
38	5149.5
39	4808.5
40	4645.5
41	4702.0
42	5030.0
43	5453.0
44	5421.0
45	5215.5
46	5596.5
47	6070.0
48	5889.5
49	5652.5
50	5788.0
51	5766.5
52	5211.5
53	4786.5
54	4465.5
55	4192.0
56	3662.5
57	2948.5
58	2647.0
59	2314.0
60	2042.5
61	1858.5
62	1579.0
63	1289.5
64	1020.5
65	938.5
66	870.5
67	734.0
68	633.0
69	523.0
70	395.0
71	286.0
72	226.0
73	197.5
74	154.5
75	101.5
76	158.0
77	154.5
78	62.0
79	32.0
80	15.5
81	8.5
82	8.0
83	3.5
84	0.0
85	0.0
86	0.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04149648169115947
2	0.2614278346543047
3	0.005335261931720504
4	0.002964034406511391
5	5.928068813022781E-4
6	0.0
7	0.0
8	5.928068813022781E-4
9	0.0
10-11	5.928068813022781E-4
12-13	0.0
14-15	2.9640344065113905E-4
16-17	0.0
18-19	5.928068813022781E-4
20-21	0.0
22-23	0.0
24-25	2.9640344065113905E-4
26-27	0.0
28-29	5.928068813022781E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	2.9640344065113905E-4
70-71	0.0
72-73	2.9640344065113905E-4
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	2.9640344065113905E-4
88-89	0.0020748240845579733
90-91	0.0020748240845579733
92-93	2.9640344065113905E-4
94-95	5.928068813022781E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	168689.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.52269561145066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.8701680786728	32.15028840054776
2	10.286305810564167	8.953755135189608
3	4.430793538369338	5.785202354628933
4	2.7036966411506715	4.706886637540088
5	1.7883897681767413	3.8917771757494566
6	1.3048571195074778	3.407453953725495
7	0.9248413195674086	2.817611106829728
8	0.7940831948568471	2.7648512943938255
9	0.5734288594077747	2.246145273254332
>10	3.196763736413413	24.106491828157143
>50	0.08989621073851099	2.5911588781722577
>100	0.028603339780435316	2.2301394874591702
>500	0.004086191397205045	1.2822412842568278
>1k	0.004086191397205045	3.065997190095383
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1914	1.1346323708125603	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1710	1.0136997670268957	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT	1548	0.9176650522559266	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	923	0.5471607514420027	No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	721	0.4274137614189426	RNA PCR Primer, Index 33 (95% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	519	0.30766677139588233	No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT	495	0.29343940624462767	RNA PCR Primer, Index 33 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	298	0.1766564506280789	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	291	0.17250680245896294	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	278	0.16480031300203335	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	262	0.1553154029011969	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	186	0.11026207992222373	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	182	0.10789085239701462	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	5.928068813022782E-4	0.0
14-15	0.0	0.0	0.0	5.928068813022782E-4	0.0
16-17	0.0	0.0	0.0	8.892103219534174E-4	0.0
18-19	0.0	0.0	0.0	0.002667630965860252	0.0
20-21	0.0	0.0	0.0	0.0035568412878136694	0.0
22-23	0.0	0.0	0.0	0.007113682575627338	0.0
24-25	0.0	0.0	0.0	0.014820172032556953	0.0
26-27	0.0	0.0	0.0	0.033789992234229856	0.0
28-29	0.0	0.0	0.0	0.07113682575627338	0.0
30-31	0.0	0.0	0.0	0.1493873340881741	0.0
32-33	0.0	0.0	0.0	0.26972713099253653	0.0
34-35	0.0	0.0	0.0	0.47098506719466	0.0
36-37	0.0	0.0	0.0	0.7368589534587318	0.0
38-39	0.0	0.0	0.0	1.0273343252968479	0.0
40-41	0.0	0.0	0.0	1.3409291655057531	0.0
42-43	0.0	0.0	0.0	1.6106562964982896	0.0
44-45	0.0	0.0	0.0	1.8877935135071047	0.0
46-47	0.0	0.0	0.0	2.2369567665941466	0.0
48-49	0.0	0.0	0.0	2.5659645857169107	0.0
50-51	0.0	0.0	0.0	2.8454730302509352	0.0
52-53	0.0	0.0	0.0	3.1226102472597503	0.0
54-55	0.0	0.0	0.0	3.461102976483351	0.0
56-57	0.0	0.0	0.0	3.836349732347693	0.0
58-59	0.0	0.0	0.0	4.222563415516127	0.0
60-61	0.0	0.0	0.0	4.601959819549585	0.0
62-63	0.0	0.0	0.0	4.963275613703324	0.0
64-65	0.0	0.0	0.0	5.405805950595475	0.0
66-67	0.0	0.0	0.0	5.868491721451902	0.0
68-69	0.0	0.0	0.0	6.411206421284138	0.0
70-71	0.0	0.0	0.0	7.000456461298603	0.0
72-73	0.0	0.0	0.0	7.562140981332512	0.0
74-75	0.0	0.0	0.0	8.12649313233228	0.0
76-77	0.0	0.0	0.0	8.794290084119297	0.0
78-79	0.0	0.0	0.0	9.476610804498218	0.0
80-81	0.0	0.0	0.0	10.155374683589327	0.0
82-83	0.0	0.0	0.0	10.861407679220342	0.0
84-85	0.0	5.928068813022782E-4	0.0	11.611604787508373	0.0
86-87	0.0	5.928068813022782E-4	0.0	12.377214874710265	0.0
88	0.0	5.928068813022782E-4	0.0	13.019224727160633	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGGTC	65	7.3123374E-10	57.840862	8
CTAGCAC	25	0.0052240756	56.411564	3
GGTATCA	975	0.0	56.162598	1
TTCGTGG	35	2.9316387E-4	53.70937	8
GTACCCA	45	1.5669897E-5	52.4507	1
TCTACAC	75	2.59206E-9	50.143612	3
CTACACT	75	1.4685793E-7	43.87566	4
CTCTACA	100	6.7484507E-10	42.44714	2
TACACTG	80	2.443703E-7	41.121235	5
GTATCAA	1590	0.0	38.89269	1
TCCGCAG	25	0.0017010875	37.59656	92-93
TACCCAT	65	1.3798861E-4	36.27961	2
ACTATAC	55	0.0027116858	34.17869	8
CTGGTCC	145	7.348717E-10	32.410828	9
CTATACA	75	3.1812428E-4	31.47042	1
TGTATAG	60	0.004146848	31.339758	3
CTAACCA	60	0.004146848	31.339758	4
ATATAGT	75	3.2598697E-4	31.339758	4
GTATAGC	60	0.004146848	31.339758	4
TATCAAC	2050	0.0	29.908556	2
>>END_MODULE