FastQCFastQC Report
Wed 25 May 2016
SRR1033015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933198
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22150.033.7469181
TCAACGC31200.032.5430344
GTATCAA34300.032.072481
ATCAACG32200.031.6783663
CAACGCA33150.030.9128655
GTACATG75650.029.6428681
AACGCAG35250.028.6658026
TACATGG76800.027.4817242
TATCAAC39900.026.5074842
ACATGGG81650.025.0433223
ACGCAGA42800.023.3890847
CGCAGAG44600.022.4631448
CATGGGG56000.020.1456874
GAGTACA54400.019.1851331
CTGTCGC7000.018.1456749
GCAGAGT54250.017.7771249
GTCGTAC1350.00997946817.4119341
AGTACAT55300.016.9155772
ACCGACC1952.454228E-416.884548
ACGCGTT1307.2365347E-616.29209310-11
Produced by FastQC (version 0.11.5)