##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033015_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2933198
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.546132923859897	33.0	31.0	34.0	30.0	34.0
2	31.597260396331922	33.0	31.0	34.0	30.0	34.0
3	31.766588890351077	34.0	31.0	34.0	30.0	34.0
4	35.1539606259107	37.0	35.0	37.0	33.0	37.0
5	35.18242546190199	37.0	35.0	37.0	33.0	37.0
6	35.33460032360584	37.0	35.0	37.0	33.0	37.0
7	35.189158386171	37.0	35.0	37.0	33.0	37.0
8	35.20959682912643	37.0	35.0	37.0	33.0	37.0
9	36.86355575041303	39.0	38.0	39.0	34.0	39.0
10-11	36.85308100578277	39.0	38.0	39.0	34.0	39.0
12-13	36.7950319071539	39.0	38.0	39.0	33.5	39.0
14-15	38.18612296203666	41.0	39.0	41.0	34.0	41.0
16-17	38.185655895033335	41.0	39.0	41.0	33.5	41.0
18-19	38.18404843450732	40.0	38.5	41.0	33.5	41.0
20-21	38.22836303584006	40.5	39.0	41.0	34.0	41.0
22-23	38.18405405976685	40.0	39.0	41.0	34.0	41.0
24-25	38.19976182992079	40.0	38.5	41.0	34.0	41.0
26-27	38.12796050590516	40.0	38.0	41.0	33.0	41.0
28-29	38.14312859206913	40.0	38.0	41.0	33.0	41.0
30-31	38.29254349689315	40.5	39.0	41.0	34.0	41.0
32-33	38.28773986618019	41.0	39.0	41.0	34.0	41.0
34-35	38.242907911433186	41.0	38.5	41.0	33.0	41.0
36-37	38.16357555814507	40.5	38.0	41.0	33.0	41.0
38-39	38.07151221976832	40.0	38.0	41.0	33.0	41.0
40-41	38.00270915908166	40.0	38.0	41.0	33.0	41.0
42-43	37.87756094201619	40.0	38.0	41.0	33.0	41.0
44-45	37.72673494936244	40.0	38.0	41.0	32.5	41.0
46-47	37.594084170246944	40.0	38.0	41.0	32.0	41.0
48-49	37.478742655627066	40.0	38.0	41.0	32.0	41.0
50-51	37.35994501564504	40.0	37.0	41.0	31.5	41.0
52-53	37.209945936142056	40.0	37.0	41.0	31.0	41.0
54-55	37.062042180582424	40.0	37.0	41.0	31.0	41.0
56-57	36.896214814001645	40.0	36.0	41.0	31.0	41.0
58-59	36.70917374142489	40.0	36.0	41.0	30.5	41.0
60-61	36.49767744966415	39.0	35.0	41.0	30.0	41.0
62-63	36.255301210487666	39.0	35.0	41.0	30.0	41.0
64-65	35.99047950394075	39.0	35.0	41.0	30.0	41.0
66-67	35.70259150592629	38.0	35.0	40.0	30.0	41.0
68-69	35.39338121736071	37.5	35.0	40.0	29.0	41.0
70-71	35.064133242965525	37.0	34.5	40.0	29.0	41.0
72-73	34.71761163071842	36.5	34.0	39.0	29.0	41.0
74-75	34.36135900133574	36.0	34.0	39.0	29.0	41.0
76-77	33.995444051168725	35.5	34.0	38.5	28.0	40.0
78-79	33.60619177430232	35.0	34.0	37.5	28.0	39.5
80-81	33.24724242959391	35.0	34.0	37.0	27.0	39.0
82-83	33.01635978887208	35.0	34.0	36.5	27.5	39.0
84-85	32.78172441819475	35.0	34.0	36.0	27.0	37.5
86-87	32.59874290791143	35.0	34.0	36.0	27.0	37.0
88-89	32.37239064665938	35.0	33.5	35.5	27.0	37.0
90-91	32.15771574233993	35.0	33.0	35.0	27.0	36.0
92-93	31.93444339591122	35.0	33.0	35.0	26.0	36.0
94-95	31.736477217017057	35.0	33.0	35.0	26.0	36.0
96-97	31.565043512234766	35.0	33.0	35.0	25.5	36.0
98-99	31.390268403292243	35.0	33.0	35.0	25.0	35.0
100	31.251484898053253	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.13492725979531173
1101	2	-0.11071146313312852
1101	3	-0.0764474113406628
1101	4	-0.1450809458139588
1101	5	-0.14767941181937516
1101	6	-0.06390976577059604
1101	7	-0.11288583951576925
1101	8	-0.08620243200510913
1101	9	-0.09737355854939267
1101	10-11	-0.11021263465664788
1101	12-13	-0.0585763954641223
1101	14-15	0.042370387734919746
1101	16-17	0.05731626889831176
1101	18-19	0.014843615555854228
1101	20-21	0.04398852247655327
1101	22-23	0.05109798713998259
1101	24-25	-0.043832644179374824
1101	26-27	-0.05359075137346281
1101	28-29	0.007569623090468269
1101	30-31	0.06252011021973658
1101	32-33	0.02776411797616163
1101	34-35	0.07357906918675639
1101	36-37	0.015213266819827709
1101	38-39	0.02079713649381887
1101	40-41	0.020462991837895572
1101	42-43	0.019542382290573812
1101	44-45	-0.0636594648075004
1101	46-47	-0.010286009518537753
1101	48-49	-0.05018168803619005
1101	50-51	-0.11933081599660511
1101	52-53	-0.13291267836824971
1101	54-55	-0.08103857773180323
1101	56-57	-0.02503703172556726
1101	58-59	-0.05213752087793466
1101	60-61	-0.055460500888429465
1101	62-63	-0.1489455526593062
1101	64-65	-0.05687436438649485
1101	66-67	-0.1091346560933033
1101	68-69	-0.12081115991922076
1101	70-71	-0.1704941494146155
1101	72-73	-0.10544535711543546
1101	74-75	-0.16380940133506527
1101	76-77	-0.10121533183236409
1101	78-79	-0.1781062908562845
1101	80-81	-0.09427256420727304
1101	82-83	-0.13726016274436148
1101	84-85	-0.12571094104868052
1101	86-87	-0.11558444324631267
1101	88-89	-0.12932549781105251
1101	90-91	-0.08948042120830735
1101	92-93	-0.037682543734657514
1101	94-95	-0.052660548429209086
1101	96-97	0.01796455563649957
1101	98-99	-0.020197880237343924
1101	100	-0.019884948662689084
1102	1	0.021279266647614037
1102	2	0.058642567178864624
1102	3	0.08113315455822345
1102	4	-0.036259221321095936
1102	5	-0.06371019812690548
1102	6	0.05140808476227221
1102	7	0.11034056160106331
1102	8	0.12469184274899447
1102	9	0.13425355354728907
1102	10-11	0.022063112080005
1102	12-13	0.05651530088783119
1102	14-15	0.09611745689898044
1102	16-17	0.0777013177842889
1102	18-19	0.06975935173049663
1102	20-21	0.1746934144970993
1102	22-23	0.17358981502809456
1102	24-25	0.21757041635903818
1102	26-27	0.05092181913163785
1102	28-29	0.04446270472845981
1102	30-31	0.19909523130390738
1102	32-33	0.2039455596418307
1102	34-35	0.20613964794586792
1102	36-37	0.22700115620231287
1102	38-39	0.24680278912587994
1102	40-41	0.19138726563848252
1102	42-43	0.18193942101549965
1102	44-45	0.16017938628036177
1102	46-47	0.06297572054872091
1102	48-49	0.07730509111263473
1102	50-51	0.16365652181417545
1102	52-53	0.15629940046542856
1102	54-55	0.15109463074812624
1102	56-57	0.1792168672947625
1102	58-59	0.17025516590707923
1102	60-61	0.10666026299927012
1102	62-63	0.13865655289723833
1102	64-65	0.07444542251513298
1102	66-67	0.06739885262157941
1102	68-69	0.08189755594067094
1102	70-71	-0.001722618925363406
1102	72-73	0.01573294284079907
1102	74-75	0.04852064685412216
1102	76-77	-0.017638526205161043
1102	78-79	-0.05916752851865681
1102	80-81	0.046388612821331776
1102	82-83	0.046200998636820145
1102	84-85	0.008833070999841652
1102	86-87	-0.0023080926168645988
1102	88-89	-0.013096307051839062
1102	90-91	-0.030231081945252924
1102	92-93	0.007098428997256434
1102	94-95	0.09083672427361478
1102	96-97	-0.002804955357039063
1102	98-99	0.030112867831785906
1102	100	0.02245722628040525
1103	1	-0.026715518990766185
1103	2	-0.005049934807495049
1103	3	0.11556996747501458
1103	4	0.03993210943684744
1103	5	-0.023576641811111188
1103	6	0.07081764177531369
1103	7	0.1047448957570083
1103	8	0.06871918552982947
1103	9	0.18102263430770194
1103	10-11	0.15181230766079779
1103	12-13	0.1451318846388645
1103	14-15	0.23931502116553816
1103	16-17	0.26146617269675687
1103	18-19	0.30610515379670744
1103	20-21	0.23012270234495702
1103	22-23	0.1673882109459015
1103	24-25	0.20959269980518513
1103	26-27	0.11349217422576174
1103	28-29	0.15213628326023354
1103	30-31	0.1768509139553629
1103	32-33	0.09852979324235633
1103	34-35	0.11539538166319119
1103	36-37	0.16178690992244782
1103	38-39	0.18098524006559558
1103	40-41	0.13589054107835352
1103	42-43	0.13378768611849523
1103	44-45	0.12268094662636031
1103	46-47	0.13031578903714092
1103	48-49	0.10311539979542772
1103	50-51	0.18283604987624358
1103	52-53	0.19382298548663357
1103	54-55	0.27353765540775754
1103	56-57	0.20721091624519516
1103	58-59	0.145641696088866
1103	60-61	0.12369288519102639
1103	62-63	0.13977423501403052
1103	64-65	0.1545341921724699
1103	66-67	0.19951648754818052
1103	68-69	0.10430657389409248
1103	70-71	0.10191601182474841
1103	72-73	0.07673368132705605
1103	74-75	0.04072886959382771
1103	76-77	0.11155040206952549
1103	78-79	0.10837338665240992
1103	80-81	0.07916425022848728
1103	82-83	0.10275317750613766
1103	84-85	0.07721340115882214
1103	86-87	0.08687669581882318
1103	88-89	0.06412257316884507
1103	90-91	0.10367497459083097
1103	92-93	0.15759521897874507
1103	94-95	0.15745176284028162
1103	96-97	0.11837007307955005
1103	98-99	0.13384656109216309
1103	100	0.17386948125802348
1104	1	-0.0796969440214994
1104	2	-0.07105178777578303
1104	3	-0.03296109050005924
1104	4	0.026758721986276157
1104	5	-0.08186583270364167
1104	6	0.04306291996114453
1104	7	0.007229078540447631
1104	8	0.07781679752167037
1104	9	0.12015312139116929
1104	10-11	0.07785641259880549
1104	12-13	0.0679995735986978
1104	14-15	0.08318027150023966
1104	16-17	0.02587783049956016
1104	18-19	0.022569222816940737
1104	20-21	0.028946977927759576
1104	22-23	-0.024846664341971803
1104	24-25	-0.016156672081962142
1104	26-27	-0.08088923486038624
1104	28-29	-0.04213320710744739
1104	30-31	-0.0015047724590004918
1104	32-33	-0.09177643005164526
1104	34-35	-0.04440594433187073
1104	36-37	-0.01122837258445486
1104	38-39	0.003884423117916924
1104	40-41	-0.02582298521275561
1104	42-43	-0.06419340162295128
1104	44-45	-0.0729254947971043
1104	46-47	-0.0802675645807227
1104	48-49	-0.027999796525804754
1104	50-51	-0.08955081905077122
1104	52-53	-0.10567757870636285
1104	54-55	-0.03731817724391817
1104	56-57	-0.08023479904867514
1104	58-59	-0.07984683678292015
1104	60-61	-0.15491449450991013
1104	62-63	-0.09841114625495351
1104	64-65	-0.03399353772437763
1104	66-67	-0.03504875363488935
1104	68-69	-0.055473081853840256
1104	70-71	-0.08338721478364874
1104	72-73	-0.05692185762349311
1104	74-75	0.016694088935643947
1104	76-77	-0.1263738158674741
1104	78-79	-0.06026866308526024
1104	80-81	-0.15406003016600067
1104	82-83	-0.13201986218267336
1104	84-85	-0.09901344145491464
1104	86-87	-0.11322156032479569
1104	88-89	-0.14849274202917684
1104	90-91	-0.10327423435964533
1104	92-93	-0.16668741033985057
1104	94-95	-0.24477190524441284
1104	96-97	-0.25101877759894364
1104	98-99	-0.25037532313317
1104	100	-0.1875351658807709
1105	1	-0.19035056243373916
1105	2	-0.08958593562027417
1105	3	-0.06546752085131757
1105	4	-0.10991437503165002
1105	5	-0.06849036717299839
1105	6	-0.00573440340514253
1105	7	-0.037020110967361575
1105	8	-0.03378596752937568
1105	9	0.01777188194096624
1105	10-11	0.07997625157209143
1105	12-13	0.058006238389843645
1105	14-15	0.009801925708103454
1105	16-17	-0.022727754528439448
1105	18-19	0.04046201010628181
1105	20-21	0.028078210679680637
1105	22-23	-0.05014088640343317
1105	24-25	-0.06699395740200487
1105	26-27	-0.11686805489092933
1105	28-29	-0.11808402831688625
1105	30-31	-0.015612360273486559
1105	32-33	-0.0011329260460755108
1105	34-35	-0.017075779286187753
1105	36-37	-0.008423299305242438
1105	38-39	0.05979699713393671
1105	40-41	0.06840945858866121
1105	42-43	0.06851129789208699
1105	44-45	-0.03113424007482024
1105	46-47	-0.0685373647039711
1105	48-49	0.01768115087926958
1105	50-51	-0.08673251666350268
1105	52-53	-0.056302863383372426
1105	54-55	-0.07248884948355538
1105	56-57	0.01789504046047341
1105	58-59	-0.005743904274815748
1105	60-61	3.0715166001016314E-4
1105	62-63	-0.021517206861012994
1105	64-65	-0.032284136014972376
1105	66-67	-0.03106662465275889
1105	68-69	0.028667729442148016
1105	70-71	-0.09610206219677053
1105	72-73	-0.06767561536000244
1105	74-75	-0.0668145719437021
1105	76-77	-0.09499035258745891
1105	78-79	-0.1271269006331508
1105	80-81	-0.06665983457614288
1105	82-83	-0.08331330054507191
1105	84-85	-0.044117164360372385
1105	86-87	-0.10485992674764333
1105	88-89	-0.05355630873067696
1105	90-91	-0.10767266160289779
1105	92-93	-0.06578833336318723
1105	94-95	0.05932041048583159
1105	96-97	0.10264384330505649
1105	98-99	0.07184103809181153
1105	100	0.11908340711711318
1106	1	-0.00659883720291532
1106	2	0.007055365201090069
1106	3	0.01703818351933961
1106	4	0.05381931892712544
1106	5	0.06476233939160636
1106	6	0.10114499800801724
1106	7	0.16948849296889534
1106	8	0.14336228614621405
1106	9	0.22473458697440662
1106	10-11	0.17148024596998113
1106	12-13	0.19487199856750692
1106	14-15	0.21350170931142287
1106	16-17	0.2216240351609713
1106	18-19	0.18931101747619294
1106	20-21	0.18729016699774803
1106	22-23	0.1586986663267922
1106	24-25	0.1233996789723335
1106	26-27	0.05071157876587762
1106	28-29	0.05038191088860344
1106	30-31	0.12022270498280108
1106	32-33	0.057266177529875506
1106	34-35	0.0784632476033238
1106	36-37	0.11601298252654146
1106	38-39	0.09880814675602068
1106	40-41	0.1164152041828288
1106	42-43	0.11031836542262852
1106	44-45	0.07456670421133538
1106	46-47	0.07102387791192655
1106	48-49	0.07993459946712989
1106	50-51	0.07888295039890636
1106	52-53	0.05040902065969988
1106	54-55	0.060730342899624645
1106	56-57	0.07402727906859496
1106	58-59	0.08203391276345684
1106	60-61	0.13942939062147275
1106	62-63	0.1538951621204916
1106	64-65	0.13972922040353097
1106	66-67	0.09145688613441649
1106	68-69	0.09551251381104464
1106	70-71	0.1126146230472429
1106	72-73	0.12562497472515588
1106	74-75	0.1727919630004493
1106	76-77	0.1246678562990482
1106	78-79	0.12830105833177896
1106	80-81	0.19330463825006206
1106	82-83	0.15728318763983395
1106	84-85	0.15124422666018944
1106	86-87	0.0972203616670484
1106	88-89	0.18693658529920043
1106	90-91	0.08903989700676362
1106	92-93	0.1554655467331294
1106	94-95	0.08513842955144213
1106	96-97	0.038821277874774296
1106	98-99	-0.016962494923941307
1106	100	0.06480403161587134
1107	1	-0.021989880211670254
1107	2	0.007727641891325021
1107	3	-0.007575577810861489
1107	4	0.03318608886089436
1107	5	0.029528280845646293
1107	6	0.03799150764749726
1107	7	0.06484238297434075
1107	8	0.07990924814604483
1107	9	0.06739569047566363
1107	10-11	0.07853783850001861
1107	12-13	0.10732020824837463
1107	14-15	0.11932095453406077
1107	16-17	0.11053403318879162
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2215	92-93	-0.17129235315292135
2215	94-95	-0.25431357457672377
2215	96-97	-0.21300955091976803
2215	98-99	-0.10181863556786297
2215	100	-0.050865691403416236
2216	1	-0.12826114446735204
2216	2	-0.16045702717975985
2216	3	-0.12933672866047274
2216	4	-0.128684107679085
2216	5	-0.12247231833546834
2216	6	-0.30381198797119424
2216	7	-0.24285623749188545
2216	8	-0.11651751646378017
2216	9	-0.20950434482262636
2216	10-11	-0.2519115786496826
2216	12-13	-0.2571923918582186
2216	14-15	-0.3082272378538349
2216	16-17	-0.3319213438025841
2216	18-19	-0.31659637761852366
2216	20-21	-0.24456464749961526
2216	22-23	-0.21407223151383192
2216	24-25	-0.307286540444494
2216	26-27	-0.3552248220100509
2216	28-29	-0.2939272989239825
2216	30-31	-0.47792834893513714
2216	32-33	-0.49341458555585405
2216	34-35	-0.6471478858687192
2216	36-37	-0.655569464503408
2216	38-39	-0.5558499520355227
2216	40-41	-0.4373749864304983
2216	42-43	-0.5265523715033282
2216	44-45	-0.4520764310463079
2216	46-47	-0.4056065752444411
2216	48-49	-0.37834372623311197
2216	50-51	-0.3023152373725537
2216	52-53	-0.4635804387312561
2216	54-55	-0.5197991920154266
2216	56-57	-0.571568163246603
2216	58-59	-0.49253797801506494
2216	60-61	-0.4193788501204665
2216	62-63	-0.5192353254322981
2216	64-65	-0.41838671889529877
2216	66-67	-0.5056588272749849
2216	68-69	-0.4419490088787299
2216	70-71	-0.47565090954130085
2216	72-73	-0.4236370834049552
2216	74-75	-0.5043737538822342
2216	76-77	-0.3796810616099151
2216	78-79	-0.368418246904362
2216	80-81	-0.3482828984401465
2216	82-83	-0.50087745858891
2216	84-85	-0.3212097532068867
2216	86-87	-0.3372179263703856
2216	88-89	-0.39503406690560894
2216	90-91	-0.4159269789930633
2216	92-93	-0.5706921869854575
2216	94-95	-0.53293500284245
2216	96-97	-0.45923654409711645
2216	98-99	-0.38519302390380616
2216	100	-0.4219774848775053
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	31924.0
3	6204.0
4	3327.0
5	2678.0
6	2396.0
7	2626.0
8	3226.0
9	3538.0
10	4187.0
11	4395.0
12	5046.0
13	5642.0
14	6284.0
15	6308.0
16	6348.0
17	6358.0
18	6178.0
19	6007.0
20	6176.0
21	6345.0
22	7296.0
23	10329.0
24	16045.0
25	21627.0
26	22321.0
27	19636.0
28	20926.0
29	26498.0
30	31963.0
31	39473.0
32	51646.0
33	70156.0
34	101425.0
35	158447.0
36	286353.0
37	585405.0
38	939789.0
39	397995.0
40	675.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.51294494573169	20.14570931207152	13.515788927879793	23.825556814316997
2	15.063362159378299	20.55498805404585	40.89544348395525	23.48620630262061
3	17.90135522210272	25.784730188715933	30.155457063825303	26.15845752535605
4	11.77281802760313	16.984911490113177	39.92791469860511	31.31435578367859
5	12.444937340578907	38.40728197166037	36.201363236962656	12.946417450798064
6	30.290025988774598	39.00431872283736	18.16265915394527	12.542996134442769
7	26.68424096928251	32.835417509124134	23.110992811598358	17.369348709995
8	24.55094500351872	36.985647304714405	20.84557149692735	17.61783619483953
9	24.641238786685786	17.204703261391117	21.418001775974655	36.73605617594845
10-11	23.28022807712398	27.316218447974556	29.572877565880447	19.83067590902102
12-13	24.071052892048616	24.770745376169394	29.302099367561684	21.856102364220305
14-15	21.33215769663488	26.83306578895501	26.268995665081423	25.56578084932869
16-17	21.10072383045001	30.099541613853596	26.827343492776194	21.972391062920195
18-19	21.018533006825972	29.147402510643882	28.683305209585203	21.150759272944946
20-21	22.2632272493904	28.476560960374176	28.10045183259143	21.159759957644
22-23	21.904468535937767	28.214780774176447	28.09651220286386	21.784238487021927
24-25	21.77601570790606	28.301323496113945	28.14456497720744	21.77809581877256
26-27	21.452540146107562	28.260509817269053	28.26220052124916	22.02474951537422
28-29	21.489735228004267	28.283432364266442	28.351134110865743	21.875698296863543
30-31	21.873877802178608	28.74702972095882	27.87187535345233	21.507217123410243
32-33	21.392376495345445	28.441241348887015	28.4636564975699	21.702725658197643
34-35	21.48333475132346	28.21342280254297	28.531694807376983	21.771547638756587
36-37	21.907670266790035	28.50291435174328	27.918935398181034	21.67047998328565
38-39	21.701988641694772	28.66209809631529	28.117435117941792	21.51847814404815
40-41	21.50298912565963	28.372100720423553	28.380767572597254	21.744142581319554
42-43	21.438624421124317	28.24058641432981	28.366964106892873	21.953825057653
44-45	21.587966783885427	28.425032250228337	28.29806136977066	21.688939596115574
46-47	21.623247884478474	28.61149349908224	28.12930079840561	21.63595781803367
48-49	21.594957115866066	28.422503614550326	28.170717664168826	21.81182160541479
50-51	21.586617605164083	28.311984073776493	28.196648601252466	21.90474971980696
52-53	21.639269647098	28.280490070488963	28.198108796167165	21.882131486245875
54-55	21.733637011530877	28.711457836163202	27.9998485248056	21.55505662750032
56-57	21.586504114857085	28.382432313483807	28.077357675619208	21.9537058960399
58-59	21.44641147705074	28.0068852206225	28.496291287613357	22.050412014713398
60-61	21.70621483189244	28.49509667796777	28.056977195612287	21.74171129452751
62-63	21.49210845625018	28.476188217962623	28.053920729915127	21.97778259587207
64-65	21.500932127251023	28.1660897411076	28.265929448312693	22.06704868332868
66-67	21.393738541139996	28.232277020051598	28.13859326991769	22.235391168890718
68-69	21.857657744638757	28.376610095650058	28.02609962255474	21.739632537156446
70-71	21.76033106201801	28.172627924164257	28.13921402108095	21.927826992736783
72-73	21.555192329242463	28.279351698454462	28.26597945142744	21.89947652087563
74-75	21.59769076627179	28.315131328031136	28.092180358481826	21.994997547215252
76-77	21.62009949732836	28.327709432870684	28.009326851179722	22.042864218621226
78-79	21.73163350913389	28.428609754312994	28.07781592294952	21.761940813603598
80-81	21.695414746622372	28.175142148653208	28.16265830772327	21.96678479700115
82-83	21.556650820982888	28.22756316669896	28.32505232623188	21.890733686086268
84-85	21.521592245871204	28.164636078404886	28.153875171432674	22.159896504291233
86-87	21.6630092825024	28.322802295250156	28.050493729808785	21.96369469243866
88-89	21.470555231345543	28.20008642821257	28.084803334887493	22.244555005554396
90-91	21.516866070912414	28.036335291081198	28.219809138767722	22.226989499238666
92-93	21.6573645145293	28.28402237700797	28.0518210139505	22.00679209451223
94-95	21.405343644882525	27.98079800817705	28.24399831172541	22.36986003521502
96-97	21.4284885955778	27.98591624717034	28.318398138537987	22.267197018713876
98-99	21.45938519710263	28.095647223864123	28.132091180494278	22.312876398538968
100	21.585908793837177	27.953496399972167	28.04996104574771	22.41063376044295
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	325.0
1	309.0
2	308.0
3	308.0
4	317.5
5	329.0
6	311.0
7	295.0
8	266.5
9	250.0
10	237.0
11	232.5
12	241.0
13	260.5
14	278.5
15	325.5
16	444.0
17	613.0
18	875.0
19	1249.0
20	1804.5
21	2750.0
22	4343.5
23	6652.0
24	9633.0
25	13445.5
26	18680.0
27	25488.0
28	34195.5
29	43477.0
30	52376.0
31	61814.5
32	71127.5
33	80607.5
34	89265.0
35	95575.5
36	100531.0
37	104574.0
38	107252.0
39	108195.5
40	108453.0
41	108277.5
42	106453.5
43	104771.0
44	102097.0
45	98899.0
46	96755.0
47	93464.5
48	91085.5
49	89461.0
50	86128.0
51	82729.0
52	78074.5
53	73884.0
54	70903.0
55	66805.5
56	61328.5
57	55880.5
58	51032.5
59	45453.5
60	39736.5
61	33901.0
62	28855.5
63	24600.5
64	20605.0
65	16913.0
66	13429.5
67	10268.5
68	8109.5
69	6408.0
70	4820.5
71	3629.5
72	2796.0
73	2143.5
74	1616.5
75	1187.5
76	805.5
77	580.0
78	469.0
79	346.5
80	241.5
81	129.5
82	65.5
83	43.0
84	31.5
85	24.5
86	18.5
87	14.5
88	9.5
89	10.0
90	11.0
91	8.0
92	5.5
93	2.5
94	1.5
95	1.0
96	1.5
97	2.0
98	2.5
99	2.5
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05949138107962708
2	0.042376955118611156
3	0.056218502808197734
4	0.07588986491876784
5	0.09112920437011071
6	0.09211788634793833
7	0.09487937738945683
8	0.1075958731732396
9	0.10145922641430957
10-11	0.1069310697743555
12-13	0.11141423115657381
14-15	0.1075447344502485
16-17	0.0771512867525479
18-19	0.10205584484920555
20-21	0.09540781086036469
22-23	0.059133410018689506
24-25	0.02256922308006483
26-27	0.18490057609476074
28-29	0.06678717222635498
30-31	0.0779524600794082
32-33	0.0731113276362523
34-35	0.021938512163174804
36-37	0.05405363020157521
38-39	0.07851498603231012
40-41	0.08494141888818962
42-43	0.07844680106832201
44-45	0.075378477688857
46-47	0.0824867601846176
48-49	0.07962299169711694
50-51	0.07442388819302345
52-53	0.07887295709324771
54-55	0.06903727603796267
56-57	0.0720033219714455
58-59	0.06791222413215882
60-61	0.06545756542858683
62-63	0.0721908306224128
64-65	0.06816791774711424
66-67	0.0702305129077546
68-69	0.06970207943684675
70-71	0.060803941636398226
72-73	0.059781167176576555
74-75	0.06259379694108615
76-77	0.056661705074120466
78-79	0.053235410633717874
80-81	0.04800221464763033
82-83	0.054207046370548455
84-85	0.04394520929033771
86-87	0.04193375285268843
88-89	0.04380883936236149
90-91	0.03997343513803023
92-93	0.0429565273125101
94-95	0.04126894945380435
96-97	0.04462705893021883
98-99	0.04428613411027827
100	0.05297971701876245
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2933198.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.6365447526411
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.82086298491043	51.857134189831356
2	13.543371899853875	18.049670154125497
3	4.241757375748367	8.479681655967045
4	1.7375114383815973	4.631270348877645
5	0.8768017385112187	2.9213519143748172
6	0.4821346454250008	1.927667212599709
7	0.29763662806325564	1.388343354017363
8	0.19804226493792676	1.055748180036045
9	0.13666191143225098	0.8196009819423
>10	0.6341810319081352	7.174174846919963
>50	0.025248937042825655	1.1145788067650209
>100	0.005737994557009537	0.5628501293693757
>500	5.11492281062742E-5	0.01792822517405331
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.4092481994055635E-5	0.0
3	0.0	0.0	0.0	3.4092481994055635E-5	0.0
4	0.0	0.0	0.0	3.4092481994055635E-5	0.0
5	0.0	0.0	0.0	3.4092481994055635E-5	0.0
6	0.0	0.0	0.0	3.4092481994055635E-5	0.0
7	0.0	0.0	0.0	3.4092481994055635E-5	0.0
8	0.0	0.0	0.0	6.818496398811127E-5	0.0
9	0.0	0.0	0.0	6.818496398811127E-5	0.0
10-11	0.0	0.0	0.0	6.818496398811127E-5	0.0
12-13	0.0	0.0	0.0	6.818496398811127E-5	0.0
14-15	0.0	0.0	0.0	1.5341616897325035E-4	0.0
16-17	0.0	0.0	0.0	2.5569361495541724E-4	0.0
18-19	0.0	0.0	0.0	2.727398559524451E-4	0.0
20-21	0.0	0.0	0.0	2.727398559524451E-4	0.0
22-23	0.0	3.4092481994055635E-5	0.0	2.727398559524451E-4	0.0
24-25	0.0	3.4092481994055635E-5	0.0	3.4092481994055636E-4	0.0
26-27	0.0	3.4092481994055635E-5	0.0	4.7729474791677887E-4	0.0
28-29	0.0	3.4092481994055635E-5	0.0	8.182195678573352E-4	0.0
30-31	0.0	3.4092481994055635E-5	0.0	0.003170600825447174	0.0
32-33	0.0	3.4092481994055635E-5	0.0	0.009102692692412855	0.0
34-35	0.0	3.4092481994055635E-5	0.0	0.018324709071804904	0.0
36-37	0.0	3.4092481994055635E-5	0.0	0.03468910042895161	0.0
38-39	0.0	3.4092481994055635E-5	0.0	0.06283244431504453	0.0
40-41	0.0	3.4092481994055635E-5	0.0	0.09701015751408532	0.0
42-43	0.0	3.4092481994055635E-5	0.0	0.13367662189869214	0.0
44-45	0.0	3.4092481994055635E-5	0.0	0.17622403942727358	0.0
46-47	0.0	3.4092481994055635E-5	0.0	0.22787414964826785	0.0
48-49	0.0	3.4092481994055635E-5	0.0	0.2885417213566899	0.0
50-51	0.0	3.4092481994055635E-5	0.0	0.35611302066890815	0.0
52-53	0.0	3.4092481994055635E-5	0.0	0.4247752794049362	0.0
54-55	0.0	3.4092481994055635E-5	0.0	0.49981283227385265	0.0
56-57	0.0	3.4092481994055635E-5	0.0	0.5898169847381596	0.0
58-59	0.0	3.4092481994055635E-5	0.0	0.6913614423574542	0.0
60-61	0.0	3.4092481994055635E-5	0.0	0.8002357836054709	0.0
62-63	0.0	3.4092481994055635E-5	0.0	0.9131841764517772	0.0
64-65	0.0	3.4092481994055635E-5	0.0	1.0298997885584267	0.0
66-67	0.0	3.4092481994055635E-5	0.0	1.166303809016643	0.0
68-69	0.0	3.4092481994055635E-5	0.0	1.3262486882917552	0.0
70-71	0.0	6.818496398811127E-5	0.0	1.5010067509932843	0.0
72-73	0.0	6.818496398811127E-5	0.0	1.683435622143476	0.0
74-75	0.0	6.818496398811127E-5	0.0	1.8683021057562428	0.0
76-77	0.0	6.818496398811127E-5	0.0	2.070078460438061	0.0
78-79	0.0	6.818496398811127E-5	0.0	2.30712348774273	0.0
80-81	0.0	6.818496398811127E-5	0.0	2.5674195877673447	0.0
82-83	0.0	6.818496398811127E-5	0.0	2.8385059583430783	0.0
84-85	0.0	6.818496398811127E-5	0.0	3.1078877048191087	0.0
86-87	0.0	6.818496398811127E-5	0.0	3.395577114125947	0.0
88	0.0	6.818496398811127E-5	0.0	3.633406268516479	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2215	0.0	33.746918	1
TCAACGC	3120	0.0	32.543034	4
GTATCAA	3430	0.0	32.07248	1
ATCAACG	3220	0.0	31.678366	3
CAACGCA	3315	0.0	30.912865	5
GTACATG	7565	0.0	29.642868	1
AACGCAG	3525	0.0	28.665802	6
TACATGG	7680	0.0	27.481724	2
TATCAAC	3990	0.0	26.507484	2
ACATGGG	8165	0.0	25.043322	3
ACGCAGA	4280	0.0	23.389084	7
CGCAGAG	4460	0.0	22.463144	8
CATGGGG	5600	0.0	20.145687	4
GAGTACA	5440	0.0	19.185133	1
CTGTCGC	700	0.0	18.145674	9
GCAGAGT	5425	0.0	17.777124	9
GTCGTAC	135	0.009979468	17.411934	1
AGTACAT	5530	0.0	16.915577	2
ACCGACC	195	2.454228E-4	16.88454	8
ACGCGTT	130	7.2365347E-6	16.292093	10-11
>>END_MODULE