FastQCFastQC Report
Wed 25 May 2016
SRR1033015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933198
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49800.16978056033039704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41010.13981326865762214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36400.050.4854741
GTATCAA70600.045.784941
TCAACGC84750.037.8212554
ATCAACG85350.037.610443
CAACGCA87000.036.8412325
AACGCAG90150.035.6581956
TATCAAC92400.034.966732
ACGCAGA105950.030.2075277
CGCAGAG107250.029.4908168
GTACATG74950.026.7818891
TACATGG75300.026.0821842
GCAGAGT119800.026.0483559
ACATGGG77100.024.9931353
TATACCG1652.667719E-622.7863665
AGAGTAC111650.021.72000910-11
GTGGTAT18100.021.6074121
CATGGGG52150.020.5481054
GAGTACT85750.020.25130712-13
CAGAGTA119750.019.95650310-11
ACACCGT5750.019.6160896
Produced by FastQC (version 0.11.5)