##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033015_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2933198 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6774315951395 34.0 31.0 34.0 31.0 34.0 2 32.68285332255102 34.0 31.0 34.0 31.0 34.0 3 32.78590534972409 34.0 31.0 34.0 31.0 34.0 4 36.28380729838218 37.0 37.0 37.0 35.0 37.0 5 36.27162230439268 37.0 37.0 37.0 35.0 37.0 6 36.340191831577684 37.0 37.0 37.0 35.0 37.0 7 36.288106701286445 37.0 37.0 37.0 35.0 37.0 8 36.29782101310583 37.0 37.0 37.0 35.0 37.0 9 38.09707288768095 39.0 39.0 39.0 35.0 39.0 10-11 38.05469473932547 39.0 39.0 39.0 35.0 39.0 12-13 38.03436709693652 39.0 39.0 39.0 35.0 39.0 14-15 39.51469164372811 41.0 39.0 41.0 37.0 41.0 16-17 39.51106778335455 41.0 39.0 41.0 37.0 41.0 18-19 39.534098277716 41.0 39.5 41.0 37.0 41.0 20-21 39.52823709821158 41.0 40.0 41.0 37.0 41.0 22-23 39.455563347581716 41.0 39.0 41.0 36.0 41.0 24-25 39.40281972100077 41.0 39.0 41.0 36.0 41.0 26-27 39.291228890787465 41.0 39.0 41.0 36.0 41.0 28-29 39.23656398238373 41.0 39.0 41.0 36.0 41.0 30-31 39.22788676386661 41.0 39.0 41.0 36.0 41.0 32-33 39.180134447111996 41.0 39.0 41.0 35.0 41.0 34-35 39.09525422422898 41.0 39.0 41.0 35.0 41.0 36-37 39.00068440657603 41.0 39.0 41.0 35.0 41.0 38-39 38.89744129104139 41.0 39.0 41.0 35.0 41.0 40-41 38.7924246845934 41.0 39.0 41.0 35.0 41.0 42-43 38.67280780226906 41.0 39.0 41.0 35.0 41.0 44-45 38.50062082409711 41.0 38.0 41.0 35.0 41.0 46-47 38.2987624769961 40.5 38.0 41.0 34.0 41.0 48-49 38.09402092869284 40.0 38.0 41.0 34.0 41.0 50-51 37.957899023523126 40.0 38.0 41.0 34.0 41.0 52-53 37.82638216035876 40.0 38.0 41.0 33.0 41.0 54-55 37.68190316507784 40.0 37.5 41.0 33.0 41.0 56-57 37.53707830156709 40.0 37.0 41.0 33.0 41.0 58-59 37.36655128634344 40.0 37.0 41.0 33.0 41.0 60-61 37.183367437179484 40.0 36.0 41.0 33.0 41.0 62-63 36.96323722435376 39.5 36.0 41.0 32.0 41.0 64-65 36.70451619699727 39.0 35.0 41.0 32.0 41.0 66-67 36.436940158830055 39.0 35.0 41.0 32.0 41.0 68-69 36.14034596368878 38.0 35.0 40.5 31.0 41.0 70-71 35.82020000013637 37.0 35.0 40.0 31.0 41.0 72-73 35.48044148400483 37.0 35.0 39.5 31.0 41.0 74-75 35.13694472722264 36.5 35.0 39.0 31.0 41.0 76-77 34.776449117993394 36.0 35.0 39.0 30.0 40.5 78-79 34.40918546923869 35.5 34.5 37.5 30.0 39.5 80-81 34.074611737768805 35.0 34.0 37.0 30.0 39.0 82-83 33.763798761624685 35.0 34.0 37.0 30.0 39.0 84-85 33.52603966728465 35.0 34.0 36.0 30.0 38.0 86-87 33.303366837151806 35.0 34.0 36.0 30.0 37.0 88-89 33.08430525317418 35.0 34.0 36.0 29.5 37.0 90-91 32.89003265377926 35.0 34.0 35.0 29.0 36.5 92-93 32.706735617575085 35.0 34.0 35.0 29.0 36.0 94-95 32.548082502442725 35.0 34.0 35.0 29.0 36.0 96-97 32.39851247682563 35.0 34.0 35.0 29.0 36.0 98-99 32.26547662312602 35.0 34.0 35.0 29.0 36.0 100 32.158219799686215 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12430002631698756 1101 2 0.1468643946157684 1101 3 0.15047269000128694 1101 4 0.1338197183510843 1101 5 0.1252922844207376 1101 6 0.18130412223094083 1101 7 0.17910195021742226 1101 8 0.21189128320462913 1101 9 0.26913980909365876 1101 10-11 0.2750761897428262 1101 12-13 0.2771618428773692 1101 14-15 0.37508791791798046 1101 16-17 0.3576183899106766 1101 18-19 0.3959473760205299 1101 20-21 0.3737135018103217 1101 22-23 0.3221353413769066 1101 24-25 0.3517405826980209 1101 26-27 0.37593152885154524 1101 28-29 0.38658699459288215 1101 30-31 0.37736295060066993 1101 32-33 0.3770515992459096 1101 34-35 0.3144973997296816 1101 36-37 0.28224766341089946 1101 38-39 0.30880788098803436 1101 40-41 0.26114380831177897 1101 42-43 0.22147253043897308 1101 44-45 0.2064262357155613 1101 46-47 0.15251765490424418 1101 48-49 0.0429722429306878 1101 50-51 0.15446107372584095 1101 52-53 0.16020397697766242 1101 54-55 0.10625643647890115 1101 56-57 0.14905368410699538 1101 58-59 0.19031557548898093 1101 60-61 0.1525665379856349 1101 62-63 0.15402202729957537 1101 64-65 0.1989724358117826 1101 66-67 0.15751465824675392 1101 68-69 0.17954424291052362 1101 70-71 0.05633273362804658 1101 72-73 0.13553123475204387 1101 74-75 0.1494572254767519 1101 76-77 0.10415054608382945 1101 78-79 0.1634620088292067 1101 80-81 0.0978636107659625 1101 82-83 0.10531376692404137 1101 84-85 0.14156173379190307 1101 86-87 0.17233080374411003 1101 88-89 0.18218761941540862 1101 90-91 0.16416813534671348 1101 92-93 0.14817258205184203 1101 94-95 0.1504338700087544 1101 96-97 0.19649466411370042 1101 98-99 0.21913627970926797 1101 100 0.22808329566593244 1102 1 0.21687165606958558 1102 2 0.1284444421937181 1102 3 0.1856806173776775 1102 4 0.1453230202021274 1102 5 0.12757460796547093 1102 6 0.1185921790897666 1102 7 0.14854668669278936 1102 8 0.17253434344965513 1102 9 0.20251478739508144 1102 10-11 0.22779285156042306 1102 12-13 0.23055513355517832 1102 14-15 0.30311771159202294 1102 16-17 0.3176043805760074 1102 18-19 0.3124708117059427 1102 20-21 0.3058501331643342 1102 22-23 0.2625033736888369 1102 24-25 0.29507184600750236 1102 26-27 0.32346437010597384 1102 28-29 0.2962188400354506 1102 30-31 0.24872950863183263 1102 32-33 0.2849964567443237 1102 34-35 0.25985060366207335 1102 36-37 0.2486808819149573 1102 38-39 0.3014743732511178 1102 40-41 0.32416625503107355 1102 42-43 0.27014641122026717 1102 44-45 0.23424258874101866 1102 46-47 0.2222770513048502 1102 48-49 0.2506208995748551 1102 50-51 0.2604790976028326 1102 52-53 0.30805624573195445 1102 54-55 0.22696898106795516 1102 56-57 0.21265618573897171 1102 58-59 0.24787792680789522 1102 60-61 0.2768468175855503 1102 62-63 0.2834616783620163 1102 64-65 0.2861145315397948 1102 66-67 0.3193988770857743 1102 68-69 0.3316946635573004 1102 70-71 0.2700869419294918 1102 72-73 0.3366959899065307 1102 74-75 0.3066374373526628 1102 76-77 0.2435996589482201 1102 78-79 0.26212024218609287 1102 80-81 0.2924185515624629 1102 82-83 0.22479285438581087 1102 84-85 0.2748710662581004 1102 86-87 0.2878693074102543 1102 88-89 0.30387568311999047 1102 90-91 0.18357155284199678 1102 92-93 0.29168146807747064 1102 94-95 0.29249414682147545 1102 96-97 0.29913687841984427 1102 98-99 0.31811296260198674 1102 100 0.31254149137119924 1103 1 0.17092446605352052 1103 2 0.1443613046025476 1103 3 0.18771686502909546 1103 4 0.12403862957172862 1103 5 0.1419992432072661 1103 6 0.16198781195649303 1103 7 0.1715926172639186 1103 8 0.14108899208392245 1103 9 0.15448267127321458 1103 10-11 0.23039125784642778 1103 12-13 0.21931782088118013 1103 14-15 0.3232353695866266 1103 16-17 0.30726929359877175 1103 18-19 0.24149635400407732 1103 20-21 0.27131084745027323 1103 22-23 0.2994013940632243 1103 24-25 0.27945418556347335 1103 26-27 0.25608799431177687 1103 28-29 0.27810651732346514 1103 30-31 0.28075249031925154 1103 32-33 0.27073869248434335 1103 34-35 0.30620129775792293 1103 36-37 0.3388594549491586 1103 38-39 0.2695714134088547 1103 40-41 0.2643624174201662 1103 42-43 0.2596004876253488 1103 44-45 0.35438665712252515 1103 46-47 0.3507215402868056 1103 48-49 0.3404303224958056 1103 50-51 0.39771696180061156 1103 52-53 0.4052233085130794 1103 54-55 0.35532817423590046 1103 56-57 0.30485807717906965 1103 58-59 0.38315017362117487 1103 60-61 0.42975821393105207 1103 62-63 0.34520177125786233 1103 64-65 0.3327873095745417 1103 66-67 0.3692951299671776 1103 68-69 0.3454390406532255 1103 70-71 0.3973827744150853 1103 72-73 0.3521545307149765 1103 74-75 0.3360585091455661 1103 76-77 0.3965256278246443 1103 78-79 0.36181632120170093 1103 80-81 0.3416045330853592 1103 82-83 0.3081303062677776 1103 84-85 0.32079807176856434 1103 86-87 0.3459316345591077 1103 88-89 0.3221122134040897 1103 90-91 0.32620364089778775 1103 92-93 0.3618458480866593 1103 94-95 0.3666555240211409 1103 96-97 0.40037910296692303 1103 98-99 0.39719436730218405 1103 100 0.4666140083778103 1104 1 0.05886222273538522 1104 2 0.0897999858816263 1104 3 0.10136331839684942 1104 4 0.023238192558025617 1104 5 0.0308408408357721 1104 6 0.03282983697533837 1104 7 0.04389005735615825 1104 8 0.03214637985149693 1104 9 0.05502655614765217 1104 10-11 0.08258661014155422 1104 12-13 0.11076315029724526 1104 14-15 0.18757133778613877 1104 16-17 0.14661669543343692 1104 18-19 0.17105667029180438 1104 20-21 0.1490370529882341 1104 22-23 0.16260125164694728 1104 24-25 0.11900814760404188 1104 26-27 0.10436536592721524 1104 28-29 0.08704445939650896 1104 30-31 0.09633565052646986 1104 32-33 0.13150119270841998 1104 34-35 0.081948263805387 1104 36-37 0.10995592699755719 1104 38-39 0.11823982242236042 1104 40-41 0.1041803731923423 1104 42-43 0.14759550093293683 1104 44-45 0.158786377714911 1104 46-47 0.21285330529570246 1104 48-49 0.17838083366598312 1104 50-51 0.2131815990235708 1104 52-53 0.22240694177443032 1104 54-55 0.19219266696696025 1104 56-57 0.27827861984216185 1104 58-59 0.26890368732644276 1104 60-61 0.25825380464016234 1104 62-63 0.19862221485696807 1104 64-65 0.20270223129983833 1104 66-67 0.22770129302084285 1104 68-69 0.212168436051563 1104 70-71 0.16929782162143425 1104 72-73 0.14336721307527256 1104 74-75 0.16018732393459345 1104 76-77 0.2145291335104389 1104 78-79 0.19673998532383763 1104 80-81 0.1679775009763631 1104 82-83 0.12630372438101745 1104 84-85 0.14957030455761355 1104 86-87 0.12122839016636533 1104 88-89 0.15833370420387638 1104 90-91 0.18992246404592805 1104 92-93 0.14592049846154964 1104 94-95 0.10747450369038347 1104 96-97 0.1530432166131277 1104 98-99 0.14578075958991832 1104 100 0.12440595702304336 1105 1 0.038970333015910796 1105 2 0.031108629345439454 1105 3 0.06668262552650361 1105 4 0.03931407070287207 1105 5 0.035011861386102794 1105 6 0.03629552837206518 1105 7 0.08299128180220805 1105 8 0.06872402634294161 1105 9 0.12129065603244271 1105 10-11 0.09888487126740841 1105 12-13 0.1329820233781831 1105 14-15 0.1512751866193014 1105 16-17 0.1882863763272553 1105 18-19 0.14324004247517763 1105 20-21 0.19965296006965616 1105 22-23 0.16914899030675912 1105 24-25 0.11865112914357923 1105 26-27 0.1306507663505414 1105 28-29 0.17607568678980812 1105 30-31 0.13093479180664502 1105 32-33 0.16049671825566492 1105 34-35 0.1289193004316047 1105 36-37 0.1539078802167495 1105 38-39 0.15028691804703698 1105 40-41 0.18294916187492305 1105 42-43 0.19887520781610135 1105 44-45 0.14546433098769995 1105 46-47 0.1465714549621282 1105 48-49 0.2749330601751012 1105 50-51 0.2985664488480779 1105 52-53 0.2400413428140027 1105 54-55 0.1809810816174462 1105 56-57 0.16252091011893555 1105 58-59 0.10146805782184742 1105 60-61 0.16699171424014025 1105 62-63 0.19231109302860716 1105 64-65 0.17989508155302758 1105 66-67 0.2163220831502457 1105 68-69 0.24076039007454852 1105 70-71 0.2508264701759444 1105 72-73 0.19004720932423425 1105 74-75 0.17734396997055057 1105 76-77 0.13315889933848268 1105 78-79 0.17570268928654542 1105 80-81 0.2014652297227144 1105 82-83 0.21849721097105146 1105 84-85 0.1916052093080367 1105 86-87 0.18554831353801404 1105 88-89 0.18482926324254123 1105 90-91 0.2150194216859873 1105 92-93 0.24405670133913304 1105 94-95 0.17254212285283188 1105 96-97 0.19410100939505526 1105 98-99 0.16045100313947103 1105 100 0.200999478444146 1106 1 -0.003894840395886945 1106 2 0.027284586293099267 1106 3 0.03832213285732422 1106 4 0.022029104864849103 1106 5 0.03754384173159053 1106 6 0.06697160470730523 1106 7 0.07345044750253038 1106 8 0.03227012654498651 1106 9 0.0919488295845099 1106 10-11 0.07088468281705929 1106 12-13 0.09769747941196982 1106 14-15 0.08413455661693092 1106 16-17 0.0747706851588319 1106 18-19 0.03909627627353274 1106 20-21 0.0680978056849213 1106 22-23 0.11148468248168086 1106 24-25 0.1150863123954764 1106 26-27 0.1486985813436945 1106 28-29 0.15898682709644163 1106 30-31 0.11923775358227573 1106 32-33 0.18248791034331902 1106 34-35 0.1782682901342909 1106 36-37 0.2140356409875892 1106 38-39 0.2003357438193305 1106 40-41 0.266492146221772 1106 42-43 0.22398262986885698 1106 44-45 0.2331443358702714 1106 46-47 0.24247892369918134 1106 48-49 0.3091845535905833 1106 50-51 0.27957060010946577 1106 52-53 0.26782390323321437 1106 54-55 0.33518400623837863 1106 56-57 0.24388011897010387 1106 58-59 0.36185472367475313 1106 60-61 0.330422656288448 1106 62-63 0.31221749959095746 1106 64-65 0.2866379635370748 1106 66-67 0.3147831611933185 1106 68-69 0.2735807851135945 1106 70-71 0.30011100129326707 1106 72-73 0.29728049051249883 1106 74-75 0.3656188248048906 1106 76-77 0.34344882377631336 1106 78-79 0.35217177218648743 1106 80-81 0.2997899489477618 1106 82-83 0.2777530676545581 1106 84-85 0.25715980476617517 1106 86-87 0.23089358991222753 1106 88-89 0.22777688368212523 1106 90-91 0.20772730330062927 1106 92-93 0.20307860119704912 1106 94-95 0.23723809140732044 1106 96-97 0.2627639648747362 1106 98-99 0.26169049248185416 1106 100 0.2715873043374373 1107 1 0.050172991743316686 1107 2 0.04252823284425489 1107 3 0.04181706204707325 1107 4 0.03567909182036999 1107 5 0.0968780480047613 1107 6 0.07031893071229689 1107 7 0.05687713192568111 1107 8 0.039678814129409545 1107 9 0.06357180722448419 1107 10-11 0.12627934148365938 1107 12-13 0.0972074843858266 1107 14-15 0.13159321842248062 1107 16-17 0.12638730180442082 1107 18-19 0.09082364698947742 1107 20-21 0.08556712840956493 1107 22-23 0.12755906208315793 1107 24-25 0.15880264429508628 1107 26-27 0.13544778451389305 1107 28-29 0.11236143074881255 1107 30-31 0.05717645443444752 1107 32-33 0.07770055402385623 1107 34-35 0.1171910122181643 1107 36-37 0.09136085628358614 1107 38-39 0.09481021653073185 1107 40-41 0.016115747764281707 1107 42-43 0.16881335547701326 1107 44-45 0.17340174011322773 1107 46-47 0.13093412199806664 1107 48-49 0.0895502911289654 1107 50-51 0.06357256484429286 1107 52-53 0.11812011222940555 1107 54-55 0.14459609696513098 1107 56-57 0.0777283924056249 1107 58-59 0.08115775756105847 1107 60-61 0.12444861377625216 1107 62-63 0.15889582842521577 1107 64-65 0.15612289664223056 1107 66-67 0.13049965475356373 1107 68-69 0.1438657006056019 1107 70-71 0.13229961749067343 1107 72-73 0.1025965411804961 1107 74-75 0.09214237288776417 1107 76-77 0.12293710792857127 1107 78-79 0.11618192951825534 1107 80-81 0.13205386550937703 1107 82-83 0.09090945219517721 1107 84-85 0.06186358129908598 1107 86-87 0.11296355506216571 1107 88-89 0.11434471478769836 1107 90-91 0.035709952277507284 1107 92-93 0.13228917843299826 1107 94-95 0.12307121422350775 1107 96-97 0.15766005760757196 1107 98-99 0.13854188915114918 1107 100 0.12720709920622397 1108 1 -0.06017019734543538 1108 2 0.06271120511079431 1108 3 0.015660110127733162 1108 4 -0.019115713877681628 1108 5 -0.005372637370641087 1108 6 -0.0012931671219007512 1108 7 0.009303461950274539 1108 8 5.184938278688378E-4 1108 9 -5.063150640580716E-4 1108 10-11 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-0.23418705681511653 2214 62-63 -0.2890225353910907 2214 64-65 -0.2965118641321851 2214 66-67 -0.29351369409192785 2214 68-69 -0.35597832084786774 2214 70-71 -0.29576604704567444 2214 72-73 -0.24274543222950484 2214 74-75 -0.2720365285994504 2214 76-77 -0.23568614343554373 2214 78-79 -0.21859193285447276 2214 80-81 -0.22864621935823948 2214 82-83 -0.15819132627325416 2214 84-85 -0.15857679060379581 2214 86-87 -0.1536772107125941 2214 88-89 -0.17533997058551165 2214 90-91 -0.14256119401890288 2214 92-93 -0.1797101660402376 2214 94-95 -0.2429220668877008 2214 96-97 -0.2187976383357011 2214 98-99 -0.25938361354964456 2214 100 -0.17892520256119582 2215 1 -0.19304051328064986 2215 2 -0.18480878657197763 2215 3 -0.21617261447159564 2215 4 -0.11289086721635044 2215 5 -0.11477168839744678 2215 6 -0.18021653099713575 2215 7 -0.19340850888330152 2215 8 -0.13233206042743717 2215 9 -0.23132339658160816 2215 10-11 -0.2069711774457872 2215 12-13 -0.1790535600859684 2215 14-15 -0.2987866562996828 2215 16-17 -0.2851425756731274 2215 18-19 -0.2605347541567653 2215 20-21 -0.3071021895426327 2215 22-23 -0.3292549866686514 2215 24-25 -0.3621157230518506 2215 26-27 -0.37080967796704556 2215 28-29 -0.37914052556926237 2215 30-31 -0.39687073599888123 2215 32-33 -0.36911652564329245 2215 34-35 -0.30154785146412166 2215 36-37 -0.2824676490159277 2215 38-39 -0.2819665427778517 2215 40-41 -0.3125553336295681 2215 42-43 -0.28717683537880134 2215 44-45 -0.2594735882359416 2215 46-47 -0.30495558942205747 2215 48-49 -0.2639233215384209 2215 50-51 -0.2499398068010379 2215 52-53 -0.13604923327657303 2215 54-55 -0.18243645322866087 2215 56-57 -0.18091660474716065 2215 58-59 -0.1528109964572053 2215 60-61 -0.1659681830054609 2215 62-63 -0.22093756307720014 2215 64-65 -0.1154714566706474 2215 66-67 -0.1598041715474352 2215 68-69 -0.06397059322786447 2215 70-71 -0.049018922526592235 2215 72-73 -0.03547726620024605 2215 74-75 -0.05888170911227064 2215 76-77 -0.05281465092078008 2215 78-79 -0.09078320577078358 2215 80-81 -0.14088051976589355 2215 82-83 -0.14365492260965596 2215 84-85 -0.06711702084275828 2215 86-87 -0.1259235479338443 2215 88-89 -0.062047669348679335 2215 90-91 -0.031417474750909946 2215 92-93 -0.005631705492135097 2215 94-95 -0.03408220520007177 2215 96-97 0.012834656700135838 2215 98-99 0.028216570905037486 2215 100 -0.03270491240309781 2216 1 -0.2486537602105301 2216 2 -0.2155548621734127 2216 3 -0.2529816964812852 2216 4 -0.19911520415994488 2216 5 -0.20131293916425363 2216 6 -0.1849587574373288 2216 7 -0.1909748960453328 2216 8 -0.24276949661158653 2216 9 -0.2886669318453201 2216 10-11 -0.32501498696053943 2216 12-13 -0.35282067020553853 2216 14-15 -0.41868864059462396 2216 16-17 -0.4520922003017773 2216 18-19 -0.3706455958703643 2216 20-21 -0.4326566868317201 2216 22-23 -0.40520136335521073 2216 24-25 -0.4333755455121704 2216 26-27 -0.4494996520093224 2216 28-29 -0.4471407891546093 2216 30-31 -0.4527075209641893 2216 32-33 -0.42944689726466123 2216 34-35 -0.40464107304089225 2216 36-37 -0.4874631008100323 2216 38-39 -0.47787254606995333 2216 40-41 -0.4502423994015814 2216 42-43 -0.40239732655786753 2216 44-45 -0.46616131908540837 2216 46-47 -0.37510366596234945 2216 48-49 -0.3755454311858486 2216 50-51 -0.4178254651088338 2216 52-53 -0.43770577885475603 2216 54-55 -0.4630130665509995 2216 56-57 -0.3679025538819829 2216 58-59 -0.2890940286941017 2216 60-61 -0.4430532755558545 2216 62-63 -0.5180086068004357 2216 64-65 -0.5530868516852152 2216 66-67 -0.5928313473323641 2216 68-69 -0.5189063986475304 2216 70-71 -0.533560423291874 2216 72-73 -0.4988390051570022 2216 74-75 -0.6733817902938384 2216 76-77 -0.5841094663736754 2216 78-79 -0.6162863786454054 2216 80-81 -0.5229030481607566 2216 82-83 -0.5113636458579478 2216 84-85 -0.5718349267794736 2216 86-87 -0.6020813703384604 2216 88-89 -0.6898592180552399 2216 90-91 -0.6565616824289862 2216 92-93 -0.7202496867680637 2216 94-95 -0.5966693565014545 2216 96-97 -0.6848714035209653 2216 98-99 -0.8096098282542066 2216 100 -0.816994818239273 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18.0 3 9.0 4 14.0 5 29.0 6 63.0 7 190.0 8 395.0 9 731.0 10 1389.0 11 2240.0 12 3388.0 13 5425.0 14 6439.0 15 8379.0 16 10258.0 17 14181.0 18 16279.0 19 8469.0 20 6050.0 21 5524.0 22 5674.0 23 6204.0 24 7193.0 25 8827.0 26 11058.0 27 13972.0 28 18248.0 29 23649.0 30 30295.0 31 38311.0 32 49752.0 33 65104.0 34 89308.0 35 135747.0 36 244455.0 37 548635.0 38 1023595.0 39 521895.0 40 1806.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.466741158830665 20.180895897882976 13.745850745535588 23.606512197750774 2 15.42934106892519 20.67342163919725 40.355845883278505 23.541391408599054 3 17.909551665426804 25.91112814264119 30.5360977143126 25.64322247761941 4 11.966952495149348 17.21786185384733 39.63784527759093 31.17734037341239 5 12.22812857546124 38.11634303699593 36.26085337864467 13.394675008898169 6 30.181051534877636 39.053483603902635 17.77380865526296 12.991656205956776 7 26.34820424669593 33.08320815710361 23.086167384540694 17.482420211659765 8 24.71046038256629 36.40564150101374 20.71805075087166 18.165847365548306 9 24.981675290928194 16.24128340466617 21.612144833045708 37.164896471359924 10-11 23.47023798362531 26.77349025035925 29.479985067426707 20.276286698588734 12-13 24.60459539383294 24.24730618253524 29.59479721450785 21.553301209123966 14-15 21.587202881230223 26.13150215388255 27.14662510102051 25.134669863866716 16-17 20.690271777252942 29.626490590031167 27.582761786006206 22.100475846709685 18-19 20.47935638519656 28.089450947997445 30.47469250980931 20.956500156996682 20-21 21.706247483547813 27.454463508588066 30.057783367431508 20.781505640432606 22-23 21.283768234821235 27.258110870985984 30.13293022313908 21.325190671053697 24-25 21.143485817655673 27.42459141252045 30.12523379270233 21.306688977121553 26-27 20.89578800447567 27.35567439453374 30.23286219731801 21.51567540367258 28-29 20.914788238412584 27.44695412933178 30.279277613044492 21.35898001921115 30-31 21.303476243481935 27.881712603492097 29.755386191507892 21.059424961518072 32-33 20.93123273641943 27.48701246898436 30.38238468729353 21.199370107302677 34-35 20.996911902980976 27.39281494123479 30.297750100743286 21.31252305504095 36-37 21.477036326903264 27.704437272901455 29.608366022341485 21.210160377853796 38-39 21.312369638871974 27.877541850226272 29.67552821186977 21.13456029903198 40-41 21.135478161022995 27.633594891033535 29.824362231504477 21.406564716438996 42-43 21.194392604931544 27.53417941782314 29.685619586540014 21.5858083907053 44-45 21.330609116738795 27.793640252038905 29.483587538243246 21.392163092979064 46-47 21.466314241316134 28.039225446083083 29.111774929616068 21.382685382984715 48-49 21.481502442044484 27.967443725244596 28.914737429931424 21.636316402779492 50-51 21.504088711365547 27.97659755666 28.73358702685601 21.785726705118442 52-53 21.574336270514298 27.959653593108957 28.740968049207726 21.725042087169022 54-55 21.624009016779638 28.28412879048738 28.53140156239026 21.560460630342718 56-57 21.4418358392444 28.035509365545728 28.641008892001153 21.881645903208717 58-59 21.27280531351787 27.619495854013266 29.121389009538394 21.986309822930465 60-61 21.54668726761712 28.088062926539564 28.655072040823704 21.710177765019615 62-63 21.384424099566413 28.005797767487913 28.704199307377138 21.905578825568544 64-65 21.347859912627786 27.721858531200418 28.87767890200389 22.052602654167906 66-67 21.244120111243788 27.878143220836648 28.711602270218762 22.1661343977008 68-69 21.749769534350293 27.98659276310586 28.5513699750988 21.71226772744505 70-71 21.667143393369077 27.79895111034138 28.60873306497995 21.92517243130959 72-73 21.49664954648275 27.948731498354178 28.68347888046611 21.87114007469696 74-75 21.612255852791215 27.927912724505653 28.488206288399898 21.97162513430323 76-77 21.639200906178328 27.947095277423355 28.356750610894764 22.05695320550355 78-79 21.699199985817526 28.07060757575861 28.438533641438458 21.791658796985406 80-81 21.66447679290658 27.848529148049327 28.52253751707181 21.964456541972275 82-83 21.468156486671276 27.90107239698138 28.667437952908355 21.963333163438985 84-85 21.434710510507642 27.84131858810759 28.480177608194197 22.24379329319057 86-87 21.571256598211974 27.937859954772655 28.40361709693333 22.087266350082043 88-89 21.30386836772771 27.86275866524633 28.441921980095874 22.391450986930085 90-91 21.338728970490543 27.705314886704745 28.531741303302415 22.424214839502294 92-93 21.418660182682526 27.908587824256024 28.37743115312549 22.29532083993596 94-95 21.215147201386348 27.613076680887318 28.519142478859514 22.652633638866817 96-97 21.130096110279823 27.6389074977337 28.59866364061281 22.632332751373674 98-99 21.190777219436768 27.71199717986556 28.42124121712393 22.675984383573738 100 21.2697881288614 27.589852440919433 28.304055846212904 22.836303584006263 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 24.0 1 22.5 2 73.5 3 140.0 4 108.5 5 84.0 6 198.0 7 456.0 8 782.5 9 947.0 10 854.0 11 787.0 12 866.5 13 1055.5 14 1302.5 15 1521.5 16 1776.0 17 2033.5 18 2393.5 19 2945.0 20 3583.0 21 4536.5 22 6085.0 23 8478.5 24 11397.5 25 15012.5 26 20097.0 27 26655.0 28 34740.5 29 43302.0 30 52018.5 31 61042.5 32 70092.5 33 79587.0 34 88418.5 35 94735.5 36 99618.0 37 104004.5 38 106704.0 39 107165.0 40 107371.0 41 107710.5 42 105912.5 43 104255.5 44 102030.5 45 99108.0 46 96677.0 47 93466.5 48 91007.5 49 88559.0 50 85178.0 51 82027.0 52 77402.0 53 73213.5 54 70373.0 55 66346.5 56 60861.5 57 55555.0 58 50616.5 59 44968.0 60 39336.0 61 33590.5 62 28474.0 63 24010.5 64 19927.5 65 16227.5 66 12869.5 67 9825.5 68 7663.5 69 6104.0 70 4608.5 71 3399.0 72 2526.5 73 1899.0 74 1420.5 75 998.0 76 663.5 77 483.0 78 358.5 79 241.5 80 158.0 81 79.0 82 28.0 83 13.5 84 10.5 85 5.5 86 2.0 87 0.5 88 0.0 89 0.5 90 0.5 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04588848076399889 2 0.24396580114946212 3 0.002591028631548228 4 0.00296604593348284 5 3.4092481994055636E-4 6 0.0 7 0.0 8 2.3864737395838946E-4 9 0.0 10-11 4.432022659227233E-4 12-13 0.0 14-15 2.2160113296136166E-4 16-17 1.87508650967306E-4 18-19 5.113872299108346E-4 20-21 3.4092481994055635E-5 22-23 1.7046240997027818E-4 24-25 0.001500069207738448 26-27 1.3636992797622254E-4 28-29 1.87508650967306E-4 30-31 1.0227744598216691E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.818496398811127E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.7046240997027817E-5 68-69 2.0455489196433382E-4 70-71 3.4092481994055635E-5 72-73 4.432022659227233E-4 74-75 1.1932368697919473E-4 76-77 1.7046240997027817E-5 78-79 0.0 80-81 0.0 82-83 8.523120498513909E-5 84-85 0.0 86-87 5.795721938989458E-4 88-89 0.0029830921744798677 90-91 0.0029830921744798677 92-93 7.50034603869224E-4 94-95 7.670808448662518E-4 96-97 1.7046240997027818E-4 98-99 1.5341616897325037E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2933198.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.45697372403097 #Duplication Level Percentage of deduplicated Percentage of total 1 76.22527725384096 49.132506930578025 2 14.492206720188285 18.682475755328028 3 4.510979055781295 8.72292175404447 4 1.9147939199544755 4.9368728554175965 5 0.9287574069342401 2.9932445887379733 6 0.5228553049080544 2.02210023899572 7 0.32311015799633086 1.4578692074755892 8 0.21751432119444322 1.121625190866453 9 0.15028426063389153 0.8718181774932752 >10 0.6773020187227179 7.444437686619451 >50 0.02876094834026953 1.2491178875442428 >100 0.007682136093490368 0.8426762828807802 >500 2.647196732693608E-4 0.10012585430550684 >1k 2.1177573838436652E-4 0.42220758971296946 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4980 0.16978056033039704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4101 0.13981326865762214 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.4092481994055635E-5 0.0 0.0 0.0 3 0.0 3.4092481994055635E-5 0.0 0.0 0.0 4 0.0 3.4092481994055635E-5 0.0 0.0 0.0 5 0.0 3.4092481994055635E-5 0.0 0.0 0.0 6 0.0 3.4092481994055635E-5 0.0 0.0 0.0 7 0.0 3.4092481994055635E-5 0.0 0.0 0.0 8 0.0 3.4092481994055635E-5 0.0 0.0 0.0 9 0.0 6.818496398811127E-5 0.0 3.4092481994055635E-5 0.0 10-11 0.0 6.818496398811127E-5 0.0 3.4092481994055635E-5 0.0 12-13 0.0 6.818496398811127E-5 0.0 3.4092481994055635E-5 0.0 14-15 0.0 6.818496398811127E-5 0.0 1.0227744598216691E-4 0.0 16-17 0.0 6.818496398811127E-5 0.0 2.0455489196433382E-4 0.0 18-19 0.0 6.818496398811127E-5 0.0 2.0455489196433382E-4 0.0 20-21 0.0 6.818496398811127E-5 0.0 2.3864737395838944E-4 0.0 22-23 0.0 6.818496398811127E-5 0.0 2.727398559524451E-4 0.0 24-25 0.0 6.818496398811127E-5 0.0 3.4092481994055636E-4 0.0 26-27 0.0 6.818496398811127E-5 0.0 4.943409889138067E-4 0.0 28-29 0.0 6.818496398811127E-5 0.0 8.523120498513908E-4 0.0 30-31 0.0 6.818496398811127E-5 0.0 0.003170600825447174 0.0 32-33 0.0 6.818496398811127E-5 0.0 0.009051553969421772 0.0 34-35 0.0 8.523120498513909E-5 0.0 0.018307662830807876 0.0 36-37 0.0 1.0227744598216691E-4 0.0 0.03479137787493378 0.0 38-39 0.0 1.0227744598216691E-4 0.0 0.06312223041199401 0.0 40-41 0.0 1.0227744598216691E-4 0.0 0.09741926729801398 0.0 42-43 0.0 1.0227744598216691E-4 0.0 0.13403459295962972 0.0 44-45 0.0 1.0227744598216691E-4 0.0 0.176479733042229 0.0 46-47 0.0 1.0227744598216691E-4 0.0 0.22828325943219652 0.0 48-49 0.0 1.0227744598216691E-4 0.0 0.289257663478565 0.0 50-51 0.0 1.0227744598216691E-4 0.0 0.35747671994867036 3.4092481994055635E-5 52-53 0.0 1.0227744598216691E-4 0.0 0.4262583023716776 3.4092481994055635E-5 54-55 0.0 1.0227744598216691E-4 0.0 0.501875427434493 3.4092481994055635E-5 56-57 0.0 1.0227744598216691E-4 0.0 0.5924250596107048 3.4092481994055635E-5 58-59 0.0 1.0227744598216691E-4 0.0 0.6944468119779162 3.4092481994055635E-5 60-61 0.0 1.0227744598216691E-4 0.0 0.8037473092508586 3.4092481994055635E-5 62-63 0.0 1.0227744598216691E-4 0.0 0.9173945979780431 3.4092481994055635E-5 64-65 0.0 1.0227744598216691E-4 0.0 1.0348091059655706 3.4092481994055635E-5 66-67 0.0 1.0227744598216691E-4 0.0 1.1721677159196209 3.4092481994055635E-5 68-69 0.0 1.3636992797622254E-4 0.0 1.3329308147625902 3.4092481994055635E-5 70-71 0.0 1.3636992797622254E-4 0.0 1.5090014380208905 3.4092481994055635E-5 72-73 0.0 1.3636992797622254E-4 0.0 1.6929133321378236 3.4092481994055635E-5 74-75 0.0 1.5341616897325035E-4 0.0 1.8791946537533435 3.4092481994055635E-5 76-77 0.0 1.7046240997027818E-4 0.0 2.0819767366539867 3.4092481994055635E-5 78-79 0.0 1.7046240997027818E-4 0.0 2.3209309429503224 3.4092481994055635E-5 80-81 0.0 1.7046240997027818E-4 0.0 2.5834941930275424 3.4092481994055635E-5 82-83 0.0 1.7046240997027818E-4 0.0 2.8561488177750016 3.4092481994055635E-5 84-85 0.0 1.7046240997027818E-4 0.0 3.1270817721817616 3.4092481994055635E-5 86-87 0.0 1.7046240997027818E-4 0.0 3.4161007882863688 3.4092481994055635E-5 88 0.0 1.7046240997027818E-4 0.0 3.654782254726752 3.4092481994055635E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3640 0.0 50.485474 1 GTATCAA 7060 0.0 45.78494 1 TCAACGC 8475 0.0 37.821255 4 ATCAACG 8535 0.0 37.61044 3 CAACGCA 8700 0.0 36.841232 5 AACGCAG 9015 0.0 35.658195 6 TATCAAC 9240 0.0 34.96673 2 ACGCAGA 10595 0.0 30.207527 7 CGCAGAG 10725 0.0 29.490816 8 GTACATG 7495 0.0 26.781889 1 TACATGG 7530 0.0 26.082184 2 GCAGAGT 11980 0.0 26.048355 9 ACATGGG 7710 0.0 24.993135 3 TATACCG 165 2.667719E-6 22.786366 5 AGAGTAC 11165 0.0 21.720009 10-11 GTGGTAT 1810 0.0 21.607412 1 CATGGGG 5215 0.0 20.548105 4 GAGTACT 8575 0.0 20.251307 12-13 CAGAGTA 11975 0.0 19.956503 10-11 ACACCGT 575 0.0 19.616089 6 >>END_MODULE