FastQCFastQC Report
Wed 25 May 2016
SRR1033014_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033014_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850543
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16800.032.4632951
GTATCAA23000.031.888981
TCAACGC21800.031.0512684
ATCAACG22400.030.8491123
CAACGCA22750.029.9604425
AACGCAG23950.028.2569076
GTACATG54900.025.6060561
TATCAAC26950.025.4663942
ACCGTTC2101.4169927E-924.6406658
TACATGG55600.024.5186522
ACATGGG56800.023.007523
ACGCAGA29500.022.4628437
TACACCG2555.6024874E-1022.1208935
ACACCGT3500.021.4842176
CGCAGAG31650.020.6595138
GGACCGT2151.1955963E-619.6730486
TATATCG1200.00506097719.586215
CATGGGG38500.019.0474664
GAGTACA40400.018.969251
TACTCCG2957.147719E-817.5279985
Produced by FastQC (version 0.11.5)