##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033014_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1850543
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.332581301812496	31.0	31.0	34.0	30.0	34.0
2	31.500819489198577	33.0	31.0	34.0	30.0	34.0
3	31.73145611855547	34.0	31.0	34.0	30.0	34.0
4	35.107661913287075	37.0	35.0	37.0	33.0	37.0
5	35.23290623346769	37.0	35.0	37.0	33.0	37.0
6	35.25956057222123	37.0	35.0	37.0	33.0	37.0
7	35.14594635196264	37.0	35.0	37.0	33.0	37.0
8	35.183265668509186	37.0	35.0	37.0	33.0	37.0
9	36.81056749289262	39.0	38.0	39.0	33.0	39.0
10-11	36.81005358967611	39.0	38.0	39.0	33.0	39.0
12-13	36.74862675441749	39.0	38.0	39.0	33.0	39.0
14-15	38.13239195198382	41.0	39.0	41.0	33.0	41.0
16-17	38.137971125231886	41.0	38.5	41.0	33.0	41.0
18-19	38.12414086027723	41.0	38.5	41.0	33.5	41.0
20-21	38.14330064202777	40.0	39.0	41.0	34.0	41.0
22-23	38.102097060160176	40.0	38.5	41.0	33.0	41.0
24-25	38.10850626005448	40.0	38.0	41.0	33.0	41.0
26-27	38.0135357568022	40.0	38.0	41.0	33.0	41.0
28-29	38.037846459120374	40.0	38.0	41.0	33.0	41.0
30-31	38.18583977783818	40.0	39.0	41.0	33.0	41.0
32-33	38.17855164673288	41.0	38.5	41.0	33.0	41.0
34-35	38.141166943972664	41.0	38.0	41.0	33.0	41.0
36-37	38.063866119295795	40.0	38.0	41.0	33.0	41.0
38-39	37.97226030413776	40.0	38.0	41.0	33.0	41.0
40-41	37.89263124391057	40.0	38.0	41.0	33.0	41.0
42-43	37.76858927352674	40.0	38.0	41.0	32.5	41.0
44-45	37.62254619319843	40.0	38.0	41.0	32.0	41.0
46-47	37.48830019080886	40.0	38.0	41.0	32.0	41.0
48-49	37.367782050998	40.0	37.5	41.0	31.5	41.0
50-51	37.23674213460589	40.0	37.0	41.0	31.0	41.0
52-53	37.073544359682536	40.0	37.0	41.0	31.0	41.0
54-55	36.910863460076314	40.0	36.0	41.0	31.0	41.0
56-57	36.73897769465503	40.0	36.0	41.0	30.0	41.0
58-59	36.53666383326407	39.5	35.5	41.0	30.0	41.0
60-61	36.3097758333635	39.0	35.0	41.0	30.0	41.0
62-63	36.059291786248686	39.0	35.0	41.0	30.0	41.0
64-65	35.7788708503396	38.5	35.0	40.5	29.5	41.0
66-67	35.47913639402057	38.0	35.0	40.0	29.0	41.0
68-69	35.15773100111697	37.0	34.5	40.0	29.0	41.0
70-71	34.82212572201781	37.0	34.0	39.5	29.0	41.0
72-73	34.47018902019569	36.0	34.0	39.0	28.5	41.0
74-75	34.11589193009836	36.0	34.0	39.0	28.0	40.5
76-77	33.75419403926307	35.0	34.0	38.5	28.0	40.0
78-79	33.37101272437333	35.0	34.0	37.0	27.0	39.0
80-81	33.02027648101125	35.0	33.0	37.0	27.0	39.0
82-83	32.80919411221463	35.0	33.0	36.0	27.0	39.0
84-85	32.59270333086019	35.0	33.0	36.0	27.0	37.0
86-87	32.42426439158669	35.0	33.0	36.0	27.0	37.0
88-89	32.211232594973474	35.0	33.0	35.0	27.0	37.0
90-91	32.00474590431025	35.0	33.0	35.0	26.0	36.0
92-93	31.79624629095352	35.0	33.0	35.0	26.0	36.0
94-95	31.597577305688112	35.0	33.0	35.0	25.0	36.0
96-97	31.426158970637267	35.0	33.0	35.0	25.0	36.0
98-99	31.2509166228507	35.0	33.0	35.0	25.0	35.0
100	31.110598348700893	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.02812123437765024
1101	2	0.12345291301309302
1101	3	0.1472384108312923
1101	4	-0.023759554195017074
1101	5	0.09181586351120075
1101	6	0.15307488559451343
1101	7	0.09644800729778069
1101	8	0.25572551199842763
1101	9	0.2425978579198329
1101	10-11	0.23447303935137853
1101	12-13	0.25277026659725976
1101	14-15	0.32611890259563125
1101	16-17	0.3117523794351271
1101	18-19	0.3698999477380127
1101	20-21	0.33011079128670673
1101	22-23	0.3402434770380225
1101	24-25	0.3331299224452948
1101	26-27	0.21733553539025507
1101	28-29	0.26039754676556726
1101	30-31	0.28280333793711065
1101	32-33	0.2973468041967138
1101	34-35	0.2732963948740448
1101	36-37	0.2656994698537005
1101	38-39	0.29961945622963526
1101	40-41	0.3041264373103232
1101	42-43	0.23503875122835183
1101	44-45	0.20302908763872551
1101	46-47	0.21365494398342122
1101	48-49	0.23501765597431046
1101	50-51	0.258270934123189
1101	52-53	0.11416146335436395
1101	54-55	0.11710358946719168
1101	56-57	0.2152163191699472
1101	58-59	0.13444679794394432
1101	60-61	0.1485335019408609
1101	62-63	0.26103147775015856
1101	64-65	0.1913438753189709
1101	66-67	0.21399367682094095
1101	68-69	0.2412306229032808
1101	70-71	0.2533364658332147
1101	72-73	0.19397730119676027
1101	74-75	0.18656731016554318
1101	76-77	0.19935870972762615
1101	78-79	0.2154860350664336
1101	80-81	0.20443259134558645
1101	82-83	0.21317173776143505
1101	84-85	0.21030440612580037
1101	86-87	0.1910490825508262
1101	88-89	0.124340971089822
1101	90-91	0.0961371203817798
1101	92-93	0.22404278640042108
1101	94-95	0.2020210172622683
1101	96-97	0.18705792903017837
1101	98-99	0.1307832770988533
1101	100	0.01779222241173173
1102	1	0.05467368691966712
1102	2	0.20206244270463358
1102	3	0.12312605130049903
1102	4	0.11295823697314233
1102	5	0.12096765075725813
1102	6	0.11667936090193365
1102	7	0.1720253464130792
1102	8	0.22443231988872725
1102	9	0.2617191780950492
1102	10-11	0.24184060700930132
1102	12-13	0.2543789596100723
1102	14-15	0.28498637743587807
1102	16-17	0.29237592063606144
1102	18-19	0.3127686912330816
1102	20-21	0.31614129178950634
1102	22-23	0.24701010525794231
1102	24-25	0.31669547591575054
1102	26-27	0.23404997456476195
1102	28-29	0.19096992827346781
1102	30-31	0.3249517987033812
1102	32-33	0.2801108704729316
1102	34-35	0.32075855737623016
1102	36-37	0.36596015114420766
1102	38-39	0.33950136223832317
1102	40-41	0.2941656934668586
1102	42-43	0.31091213912615245
1102	44-45	0.2648172838702365
1102	46-47	0.21615530249717096
1102	48-49	0.1987466213344078
1102	50-51	0.2539113628616505
1102	52-53	0.23221036551046126
1102	54-55	0.23972607140294144
1102	56-57	0.2989979046717437
1102	58-59	0.22690441633925928
1102	60-61	0.21337324409248026
1102	62-63	0.23147432801810197
1102	64-65	0.25489934641484524
1102	66-67	0.23133606493605186
1102	68-69	0.21077454941631402
1102	70-71	0.06664339671792874
1102	72-73	0.12272720112083135
1102	74-75	0.08364218983248861
1102	76-77	0.03842738336749818
1102	78-79	0.059187014358855095
1102	80-81	0.1780457589619857
1102	82-83	0.0921102203200661
1102	84-85	0.08120338266800076
1102	86-87	0.11627147105304658
1102	88-89	0.16523573344579745
1102	90-91	0.20510418726417257
1102	92-93	0.25662068946006045
1102	94-95	0.2616763865666272
1102	96-97	0.21905209251420743
1102	98-99	0.25710240976829724
1102	100	0.2318698039087259
1103	1	-0.14642134986167576
1103	2	-0.06421766434264597
1103	3	-0.023378751605807935
1103	4	0.015219416820329457
1103	5	0.012478853229168863
1103	6	0.0074019300366359175
1103	7	0.13144305058508365
1103	8	0.08339623668144469
1103	9	0.13513706326486385
1103	10-11	0.16679762002821974
1103	12-13	0.15703445612586364
1103	14-15	0.1765945430230218
1103	16-17	0.20339933399628052
1103	18-19	0.283159387945382
1103	20-21	0.21559054129499344
1103	22-23	0.2042073540911531
1103	24-25	0.18577587773090443
1103	26-27	0.01370452730623839
1103	28-29	0.048165884265145564
1103	30-31	0.11845268429983946
1103	32-33	0.021497313736581702
1103	34-35	0.037839579755313935
1103	36-37	0.11919871919292291
1103	38-39	0.1298638750051282
1103	40-41	0.11935735722463647
1103	42-43	0.14305929034983222
1103	44-45	0.16399826875692014
1103	46-47	0.19367602028279407
1103	48-49	0.22262863661579502
1103	50-51	0.21168392617856568
1103	52-53	0.23512572317506653
1103	54-55	0.2818371523477481
1103	56-57	0.2658850008203686
1103	58-59	0.24865001098172002
1103	60-61	0.182661793394999
1103	62-63	0.20160645642312147
1103	64-65	0.2711697571506093
1103	66-67	0.27111033480379376
1103	68-69	0.25642155196321426
1103	70-71	0.17801199876837615
1103	72-73	0.2062609248923053
1103	74-75	0.19187888326097635
1103	76-77	0.3287898961343032
1103	78-79	0.2963329373830703
1103	80-81	0.31035516179848344
1103	82-83	0.2273252845096536
1103	84-85	0.2182995312930629
1103	86-87	0.21790174423045983
1103	88-89	0.22679115109825432
1103	90-91	0.27037329222997286
1103	92-93	0.34547445615387673
1103	94-95	0.2566504650938235
1103	96-97	0.17900309381997914
1103	98-99	0.0663307126552155
1103	100	-0.01590974075248397
1104	1	-0.009903060142487874
1104	2	0.04247889722150333
1104	3	0.09810324089956168
1104	4	0.13401243646629268
1104	5	0.026268658651019905
1104	6	0.13358766515320752
1104	7	0.25129441231856475
1104	8	0.23981135764602612
1104	9	0.3096459141405461
1104	10-11	0.19260940760720047
1104	12-13	0.2649539907689231
1104	14-15	0.2299859414457046
1104	16-17	0.19014653918921454
1104	18-19	0.17918707731022465
1104	20-21	0.22413959349085388
1104	22-23	0.16304568645760043
1104	24-25	0.18799447632918742
1104	26-27	0.1907259606934062
1104	28-29	0.15348660111552448
1104	30-31	0.21980265842925206
1104	32-33	0.16846575162366406
1104	34-35	0.23324480718127205
1104	36-37	0.2468778925885573
1104	38-39	0.2365212387141895
1104	40-41	0.24259541363819181
1104	42-43	0.23868245785011055
1104	44-45	0.23524429017462722
1104	46-47	0.10914584577395203
1104	48-49	0.1709619438172254
1104	50-51	0.14202933767764847
1104	52-53	0.10659987610942068
1104	54-55	0.1225605879613525
1104	56-57	0.13632041059474176
1104	58-59	0.0986750976089823
1104	60-61	0.024251501606158854
1104	62-63	0.05579043547579232
1104	64-65	0.16424584802691555
1104	66-67	0.09448479975306867
1104	68-69	0.09108891896489268
1104	70-71	0.10322249125022154
1104	72-73	0.10126947002471098
1104	74-75	0.09981096121767052
1104	76-77	0.023411166458394916
1104	78-79	0.08934866356253224
1104	80-81	0.06891597335086885
1104	82-83	0.02785915929602112
1104	84-85	0.06166882212546909
1104	86-87	0.03763080865605417
1104	88-89	-0.011425973660678324
1104	90-91	0.11790147548778762
1104	92-93	0.07396659306414577
1104	94-95	0.12545132431080575
1104	96-97	0.09084711771498988
1104	98-99	0.12183487546742455
1104	100	0.10541546033251237
1105	1	-0.14250732311899839
1105	2	-0.11784733735965247
1105	3	-0.04331681652029218
1105	4	-0.03473094418573197
1105	5	-0.04105751539560032
1105	6	0.004201562951251958
1105	7	-0.04525405851180864
1105	8	0.08406311198342564
1105	9	0.040771168372657485
1105	10-11	0.027667026654576432
1105	12-13	0.015482309807659078
1105	14-15	0.07513077420223624
1105	16-17	0.01983721547380668
1105	18-19	-0.001585925425217738
1105	20-21	0.03572940889156939
1105	22-23	0.04459499287151658
1105	24-25	-0.03216930580650512
1105	26-27	-0.10677761058549606
1105	28-29	-0.016782430916556734
1105	30-31	-0.011664784079755464
1105	32-33	-0.029522083004259514
1105	34-35	0.0017134671070522245
1105	36-37	-0.01709045147111965
1105	38-39	0.016316130116784677
1105	40-41	-0.021809412878631917
1105	42-43	0.007785630946280264
1105	44-45	-0.04690058293825672
1105	46-47	-0.09915743587048098
1105	48-49	-0.10062482674809559
1105	50-51	-0.1586700172932396
1105	52-53	-0.11161005041613947
1105	54-55	-0.18575014408743584
1105	56-57	-0.18474124874483522
1105	58-59	-0.16806114928085947
1105	60-61	-0.12031712774191305
1105	62-63	-0.20289187255600893
1105	64-65	-0.1886155211608056
1105	66-67	-0.13658677535037356
1105	68-69	-0.10972140305296563
1105	70-71	-0.15250533047402115
1105	72-73	-0.011041171998364518
1105	74-75	-0.030171561375425426
1105	76-77	-0.038017158840752074
1105	78-79	-0.00769149515177503
1105	80-81	0.03629135110965365
1105	82-83	0.1137036626308543
1105	84-85	0.0686421936559185
1105	86-87	-0.06304174555469189
1105	88-89	-0.034131145638262694
1105	90-91	-0.04121038198598015
1105	92-93	0.0031966765552375875
1105	94-95	0.039297486912776236
1105	96-97	0.07737333304926963
1105	98-99	0.014255628258354136
1105	100	0.016378601009080285
1106	1	-0.15708291355768367
1106	2	-0.11533255249725727
1106	3	-0.008723558699255562
1106	4	0.039620966728804774
1106	5	-0.03457109090756205
1106	6	0.023079301848270006
1106	7	0.023234716261264055
1106	8	0.1234751584015541
1106	9	0.10749068962282138
1106	10-11	0.1599097982201485
1106	12-13	0.1339433158020924
1106	14-15	0.1771888330841236
1106	16-17	0.18036465122831657
1106	18-19	0.1617697000665217
1106	20-21	0.13671042148612145
1106	22-23	0.14649584625841072
1106	24-25	0.050815576306987964
1106	26-27	0.006124357577000694
1106	28-29	-0.001588079649557983
1106	30-31	0.043887110334495105
1106	32-33	0.02040084857386404
1106	34-35	0.02268518319743862
1106	36-37	0.08702385528535217
1106	38-39	0.16694163191672828
1106	40-41	0.16484431850069114
1106	42-43	0.013881431220347906
1106	44-45	0.039955075755280234
1106	46-47	-0.04480960978352755
1106	48-49	0.05316088279450781
1106	50-51	0.0881458797288488
1106	52-53	0.12466502951971137
1106	54-55	0.057563969092463196
1106	56-57	0.07795265480091729
1106	58-59	0.06860983697359302
1106	60-61	0.06755375146188669
1106	62-63	0.03760914261419401
1106	64-65	0.05465205919258409
1106	66-67	0.09594631074693183
1106	68-69	0.05122507966901679
1106	70-71	-0.053838382765846404
1106	72-73	0.03577990651685781
1106	74-75	0.08044652535572538
1106	76-77	0.08308364182570216
1106	78-79	0.0881263859294279
1106	80-81	0.04470750448989236
1106	82-83	0.04852123382858764
1106	84-85	0.0548875931943158
1106	86-87	-0.006603212148654336
1106	88-89	-0.011165560813694242
1106	90-91	-0.08404347150407077
1106	92-93	0.006663749424138388
1106	94-95	-0.007380723248584076
1106	96-97	-0.07722506602027934
1106	98-99	-0.14485620345480044
1106	100	-0.1733490637372448
1107	1	-0.06753294312309777
1107	2	-0.015244344943173616
1107	3	0.07461718634915115
1107	4	0.10148549360889803
1107	5	0.07157827062612654
1107	6	0.11824132519761577
1107	7	0.07416590459538952
1107	8	0.18919736080490424
1107	9	0.2345608793790177
1107	10-11	0.17567891433647986
1107	12-13	0.20322057584578346
1107	14-15	0.2099995997812485
1107	16-17	0.16029120194644264
1107	18-19	0.12986006760613833
1107	20-21	0.1481141188731243
1107	22-23	0.10932137122774321
1107	24-25	0.12443553125027051
1107	26-27	0.06476368886111317
1107	28-29	0.08013292058989663
1107	30-31	0.10561847068557029
1107	32-33	0.035619339418360596
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2216	48-49	-0.6415496148865358
2216	50-51	-0.6020693160142159
2216	52-53	-0.6751777243815766
2216	54-55	-0.8190404373111946
2216	56-57	-0.7583880119998199
2216	58-59	-0.725717227885923
2216	60-61	-0.6870538534354438
2216	62-63	-0.7220292616167328
2216	64-65	-0.7652120179998363
2216	66-67	-0.7675752948933763
2216	68-69	-0.6923428040119006
2216	70-71	-0.731948022070263
2216	72-73	-0.881811128040404
2216	74-75	-0.9074365012869663
2216	76-77	-0.7557346060988337
2216	78-79	-0.7815904349680949
2216	80-81	-0.6912193868324508
2216	82-83	-0.8539334915022998
2216	84-85	-0.7748270892265268
2216	86-87	-0.7076250702785067
2216	88-89	-0.8935935837283324
2216	90-91	-0.8320092839803017
2216	92-93	-1.1077605612354908
2216	94-95	-1.0316326735656638
2216	96-97	-0.9989530869149945
2216	98-99	-0.9052247415886256
2216	100	-0.8237608192992631
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	21632.0
3	4061.0
4	2369.0
5	1917.0
6	1770.0
7	1788.0
8	2135.0
9	2325.0
10	2674.0
11	3098.0
12	3301.0
13	3662.0
14	3954.0
15	4105.0
16	4097.0
17	4226.0
18	4146.0
19	4013.0
20	4171.0
21	4306.0
22	4812.0
23	6836.0
24	10430.0
25	14085.0
26	14362.0
27	12853.0
28	13746.0
29	17062.0
30	21089.0
31	25665.0
32	33350.0
33	45644.0
34	66006.0
35	102261.0
36	187085.0
37	379034.0
38	579099.0
39	232901.0
40	473.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.353130937794354	19.526739202609303	13.353866318586425	23.766263541009916
2	15.569764166728387	19.710132078093178	40.567344906497624	24.152758848680815
3	18.61472329611506	24.845494606504637	29.394901186839327	27.14488091054097
4	12.23306620574629	16.47366707516384	38.85305675226359	32.44020996682628
5	13.146298178419377	37.663073844953885	35.55719748006657	13.633430496560162
6	31.34318993750152	37.801924985599115	17.797543871984942	13.057341204914419
7	27.99445262391538	31.992825624377296	22.054876617398726	17.957845134308595
8	25.142325460623834	36.63363230155038	20.080548312758985	18.14349392506681
9	24.725502393638603	17.485624645011224	20.892813674843804	36.89605928650637
10-11	24.04500660779964	26.95481866282803	28.53720139805136	20.46297333132097
12-13	24.676809220536306	24.197848775955414	28.577251880934767	22.548090122573516
14-15	21.952256604924948	26.211667037235998	25.569202605129437	26.266873752709618
16-17	21.73788424218023	29.61848413061307	26.128022094947813	22.51560953225889
18-19	21.653314580910394	28.577772969464206	28.05133475779623	21.717577691829174
20-21	22.8579727610695	27.92693553647841	27.50954563378194	21.705546068670145
22-23	22.43685719677369	27.637652689844757	27.585636353903574	22.339853759477975
24-25	22.310683326901437	27.702531789245654	27.588595327740283	22.398189556112627
26-27	22.07633936595729	27.70439420746827	27.647682705780184	22.57158372079425
28-29	22.055289972646207	27.695438213602923	27.77246792208779	22.47680389166308
30-31	22.37322526731009	28.24048674055961	27.281271893901764	22.105016098228532
32-33	21.916644810309354	27.82026773812389	27.92150097745235	22.34158647411441
34-35	22.081981501124933	27.56821544635799	27.919410306130032	22.430392746387042
36-37	22.408836062927968	27.979874685829202	27.315212692648842	22.296076558593985
38-39	22.251615551477805	28.111028366320383	27.523215862771277	22.11414021943053
40-41	22.04708396972988	27.84815398128237	27.819705433131848	22.2850566158559
42-43	21.97136139507403	27.710817961929664	27.794427221141355	22.52339342185495
44-45	22.088093224280364	27.899441467672396	27.67552559795623	22.336939710091006
46-47	22.170283914691737	28.00742135497327	27.581458727298735	22.240836003036254
48-49	22.089385311708547	27.877029892631413	27.574956017978952	22.458628777681092
50-51	22.16627116664215	27.760911041697312	27.634176177414748	22.43864161424579
52-53	22.15563599863607	27.6940312249789	27.65557934836778	22.49475342801725
54-55	22.264047280040362	28.1635885503048	27.43382811397349	22.138536055681342
56-57	21.994809119272638	27.93503909383738	27.55230695144858	22.5178448354414
58-59	21.951503739882416	27.485837547640777	27.944616645829456	22.61804206664734
60-61	22.14303327089543	27.952543227073534	27.53836037774211	22.36606312428892
62-63	22.06167096901364	27.992285247902522	27.471488107325147	22.474555675758694
64-65	22.0827411969051	27.606535888334427	27.721340056109657	22.589382858650815
66-67	21.89274476949662	27.74611735892532	27.609627711508256	22.751510160069802
68-69	22.348560023058234	27.877209499516287	27.504297732408766	22.269932745016714
70-71	22.29552660329902	27.671854118694156	27.59974834625803	22.43287093174879
72-73	22.110140977964505	27.774321706605242	27.702163266244096	22.413374049186157
74-75	22.112095581993156	27.837049701901062	27.582776121372177	22.468078594733605
76-77	22.116809929398215	27.848571810922934	27.432449785950748	22.602168473728106
78-79	22.296712779382393	27.87265493969806	27.491070685137704	22.339561595781838
80-81	22.221798715585468	27.64710795878001	27.649135384168726	22.481957941465797
82-83	22.110583737469394	27.681808511012175	27.795486562335174	22.412121189183257
84-85	22.100821777689955	27.71746049852693	27.58222304637566	22.599494677407453
86-87	22.21820066100771	27.843253539732228	27.5619184522894	22.37662734697066
88-89	22.03786594597692	27.765468037007974	27.5334045697446	22.663261447270504
90-91	22.014184458508648	27.63423148054543	27.693319875811657	22.658264185134268
92-93	22.200227979649963	27.767753773328113	27.547748275217987	22.48426997180394
94-95	22.005751586497745	27.529550606960235	27.71141297164131	22.753284834900704
96-97	22.00637293912081	27.578099320104492	27.730800088230716	22.684727652543984
98-99	21.96260179084305	27.67732387227572	27.568359520189222	22.791714816692007
100	22.1483117927956	27.529931831551263	27.528742344025748	22.793014031627393
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	221.0
1	195.0
2	169.0
3	166.5
4	180.0
5	199.5
6	198.0
7	177.0
8	142.5
9	136.0
10	143.5
11	132.0
12	122.5
13	138.5
14	167.0
15	195.0
16	249.0
17	329.5
18	456.0
19	645.5
20	944.0
21	1439.5
22	2189.0
23	3293.0
24	4878.0
25	6891.5
26	9593.0
27	13186.5
28	17515.5
29	22200.5
30	26858.0
31	32207.5
32	37897.0
33	43135.5
34	48248.0
35	52504.0
36	56165.5
37	60551.5
38	63464.0
39	64698.0
40	66061.5
41	66640.5
42	66713.0
43	66782.5
44	65805.0
45	64970.5
46	64030.5
47	62080.5
48	60637.0
49	59462.5
50	58389.0
51	56938.0
52	53615.5
53	51253.5
54	49634.0
55	46742.5
56	43113.0
57	39897.5
58	36477.0
59	32278.5
60	28837.0
61	25174.0
62	21722.5
63	18197.0
64	14869.5
65	12493.0
66	10203.0
67	7937.0
68	6215.5
69	5064.0
70	4068.5
71	2982.5
72	2277.5
73	1868.0
74	1477.0
75	1064.0
76	699.0
77	517.5
78	401.0
79	298.0
80	191.0
81	109.0
82	75.0
83	46.0
84	23.5
85	14.5
86	16.5
87	13.0
88	9.5
89	8.0
90	5.0
91	3.5
92	3.0
93	4.5
94	3.5
95	1.0
96	0.0
97	0.0
98	0.5
99	1.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.062738342205504
2	0.04344670726375988
3	0.059063745073743214
4	0.08046286954693839
5	0.09532337265332391
6	0.09175685190779138
7	0.09408049421169894
8	0.10607697308303564
9	0.1022942995650466
10-11	0.10645524043483452
12-13	0.11134569691166323
14-15	0.11002176118036705
16-17	0.07740971163599009
18-19	0.1022942995650466
20-21	0.09683644206051953
22-23	0.06073892906028123
24-25	0.02102085712139626
26-27	0.187782721071599
28-29	0.06789898964790335
30-31	0.0770854824773053
32-33	0.07284348431784617
34-35	0.023263442135632622
36-37	0.055740396197224276
38-39	0.08019267858136774
40-41	0.08597476524457956
42-43	0.07919297200875634
44-45	0.07624789048403631
46-47	0.08400237119591385
48-49	0.08062498412628077
50-51	0.0760317377115798
52-53	0.07989546851924002
54-55	0.07057388020705274
56-57	0.07233012148326194
58-59	0.06954715453788428
60-61	0.06695332126840609
62-63	0.07341088534554452
64-65	0.07062791840016687
66-67	0.07124935762097935
68-69	0.07089810936573751
70-71	0.06357593419877301
72-73	0.06163055924666436
74-75	0.06252218943304749
76-77	0.05865845862538725
78-79	0.054956842397069404
80-81	0.04898562205795813
82-83	0.05520001426608299
84-85	0.04504083396062669
86-87	0.04277122984983327
88-89	0.04504083396062669
90-91	0.04171748508410775
92-93	0.04412218467768649
94-95	0.042825268042947395
96-97	0.045770349567667436
98-99	0.04622967420913753
100	0.05441646046592811
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1850543.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.26142502231552
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.10869696990048	55.48442508816156
2	12.446949893426115	17.24186973600102
3	3.663959689307899	7.613132079173565
4	1.4550604981917192	4.031182543937553
5	0.7507172551003759	2.59978734385466
6	0.4287518057592669	1.7817576628666714
7	0.2598527086220446	1.2598438226549942
8	0.1791983116324272	0.9929224340203909
9	0.11804170604963325	0.7358163095756596
>10	0.5615081328666133	6.6348758944739865
>50	0.0210196993433301	0.975115808588322
>100	0.006243329800191888	0.6492712766916565
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	8.105728967119382E-5	0.0
24-25	0.0	0.0	0.0	1.621145793423876E-4	0.0
26-27	0.0	0.0	0.0	2.9721006212771065E-4	0.0
28-29	0.0	0.0	0.0	7.295156070407443E-4	0.0
30-31	0.0	0.0	0.0	0.0027289287522635247	0.0
32-33	0.0	0.0	0.0	0.008186786256790575	0.0
34-35	0.0	0.0	0.0	0.01567107600309747	0.0
36-37	0.0	0.0	0.0	0.02942379615064335	0.0
38-39	0.0	0.0	0.0	0.054173288596914525	0.0
40-41	0.0	0.0	0.0	0.08394833300279972	0.0
42-43	0.0	0.0	0.0	0.11383145379491316	0.0
44-45	0.0	0.0	0.0	0.14695686617387438	0.0
46-47	0.0	0.0	0.0	0.18818800751995496	0.0
48-49	0.0	0.0	0.0	0.23876775627477986	0.0
50-51	0.0	0.0	0.0	0.2949404580169172	0.0
52-53	0.0	0.0	0.0	0.35038364415201373	0.0
54-55	0.0	0.0	0.0	0.40960950380509936	0.0
56-57	0.0	0.0	0.0	0.48380394295079876	0.0
58-59	0.0	0.0	0.0	0.5665634357050877	0.0
60-61	0.0	0.0	0.0	0.6551320342191453	0.0
62-63	0.0	5.403819311412921E-5	0.0	0.7474562871546352	0.0
64-65	0.0	5.403819311412921E-5	0.0	0.8486428037608421	0.0
66-67	0.0	5.403819311412921E-5	0.0	0.961150321824459	0.0
68-69	0.0	5.403819311412921E-5	0.0	1.0896531450498583	0.0
70-71	0.0	5.403819311412921E-5	0.0	1.2302875426293798	0.0
72-73	0.0	5.403819311412921E-5	0.0	1.3827563045008953	0.0
74-75	0.0	5.403819311412921E-5	0.0	1.5391158162766279	0.0
76-77	0.0	5.403819311412921E-5	0.0	1.7092550672964637	0.0
78-79	0.0	5.403819311412921E-5	0.0	1.897929418554446	0.0
80-81	0.0	5.403819311412921E-5	0.0	2.1131095035349086	0.0
82-83	0.0	5.403819311412921E-5	0.0	2.3367735848342894	0.0
84-85	0.0	5.403819311412921E-5	0.0	2.562329002892665	0.0
86-87	0.0	5.403819311412921E-5	0.0	2.7944500614144063	5.403819311412921E-5
88	0.0	5.403819311412921E-5	0.0	2.991608408991307	5.403819311412921E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1680	0.0	32.463295	1
GTATCAA	2300	0.0	31.88898	1
TCAACGC	2180	0.0	31.051268	4
ATCAACG	2240	0.0	30.849112	3
CAACGCA	2275	0.0	29.960442	5
AACGCAG	2395	0.0	28.256907	6
GTACATG	5490	0.0	25.606056	1
TATCAAC	2695	0.0	25.466394	2
ACCGTTC	210	1.4169927E-9	24.640665	8
TACATGG	5560	0.0	24.518652	2
ACATGGG	5680	0.0	23.00752	3
ACGCAGA	2950	0.0	22.462843	7
TACACCG	255	5.6024874E-10	22.120893	5
ACACCGT	350	0.0	21.484217	6
CGCAGAG	3165	0.0	20.659513	8
GGACCGT	215	1.1955963E-6	19.673048	6
TATATCG	120	0.005060977	19.58621	5
CATGGGG	3850	0.0	19.047466	4
GAGTACA	4040	0.0	18.96925	1
TACTCCG	295	7.147719E-8	17.527998	5
>>END_MODULE