FastQCFastQC Report
Wed 25 May 2016
SRR1033014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033014_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850543
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29320.15843998221062683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25210.13623028484071972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24450.050.5075041
GTATCAA45050.044.9890821
TCAACGC52750.037.954494
ATCAACG53350.037.440553
CAACGCA54350.036.8381465
TATCAAC56500.035.604192
AACGCAG57500.035.065276
ACGCAGA66250.030.2211827
CGCAGAG69450.028.8287038
GTGGTAT10250.028.0504251
GCAGAGT75800.026.2276239
GTACATG51450.025.7427181
TACACCG3000.025.0660825
TACATGG52150.023.7587242
ACATGGG51300.023.0871813
GAGTACT51450.021.83145312-13
AGAGTAC70450.020.74756410-11
CAGAGTA76200.020.41553710-11
TGGTATC12950.020.3722932
GGACCGT1909.047888E-619.7890136
Produced by FastQC (version 0.11.5)