##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033014_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1850543 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69802539038542 34.0 31.0 34.0 31.0 34.0 2 32.70273968235269 34.0 31.0 34.0 31.0 34.0 3 32.80427312415869 34.0 31.0 34.0 31.0 34.0 4 36.29212236624601 37.0 37.0 37.0 35.0 37.0 5 36.27599953094848 37.0 37.0 37.0 35.0 37.0 6 36.3462048706785 37.0 37.0 37.0 35.0 37.0 7 36.30035022152957 37.0 37.0 37.0 35.0 37.0 8 36.30836570671419 37.0 37.0 37.0 35.0 37.0 9 38.11076694786341 39.0 39.0 39.0 37.0 39.0 10-11 38.07061332808803 39.0 39.0 39.0 35.0 39.0 12-13 38.048922127181044 39.0 39.0 39.0 35.0 39.0 14-15 39.53924361660334 41.0 39.0 41.0 37.0 41.0 16-17 39.5311686894063 41.0 39.0 41.0 37.0 41.0 18-19 39.54837093761128 41.0 39.5 41.0 37.0 41.0 20-21 39.5388766972721 41.0 40.0 41.0 37.0 41.0 22-23 39.46720638212676 41.0 39.0 41.0 36.0 41.0 24-25 39.4118358773614 41.0 39.0 41.0 36.0 41.0 26-27 39.30328206369698 41.0 39.0 41.0 36.0 41.0 28-29 39.25143322797687 41.0 39.0 41.0 36.0 41.0 30-31 39.25129705173022 41.0 39.0 41.0 36.0 41.0 32-33 39.21494069578496 41.0 39.0 41.0 35.0 41.0 34-35 39.140527942339084 41.0 39.0 41.0 35.0 41.0 36-37 39.052612935770746 41.0 39.0 41.0 35.0 41.0 38-39 38.95398242569884 41.0 39.0 41.0 35.0 41.0 40-41 38.845592617950516 41.0 39.0 41.0 35.0 41.0 42-43 38.72524577921183 41.0 39.0 41.0 35.0 41.0 44-45 38.553726933121794 41.0 38.0 41.0 35.0 41.0 46-47 38.350826757335554 40.5 38.0 41.0 34.5 41.0 48-49 38.13781981829118 40.0 38.0 41.0 34.0 41.0 50-51 37.99647157618061 40.0 38.0 41.0 34.0 41.0 52-53 37.85375049377399 40.0 38.0 41.0 33.5 41.0 54-55 37.699033743068924 40.0 37.0 41.0 33.0 41.0 56-57 37.547110226565934 40.0 37.0 41.0 33.0 41.0 58-59 37.36166816982907 40.0 36.5 41.0 33.0 41.0 60-61 37.16294433579766 40.0 36.0 41.0 33.0 41.0 62-63 36.927446160397245 39.0 35.5 41.0 32.5 41.0 64-65 36.66029916624472 39.0 35.0 41.0 32.0 41.0 66-67 36.381623393782256 38.5 35.0 41.0 32.0 41.0 68-69 36.07250763694764 37.5 35.0 40.0 31.0 41.0 70-71 35.74556116772212 37.0 35.0 40.0 31.0 41.0 72-73 35.402447011498786 36.5 35.0 39.0 31.0 41.0 74-75 35.055448049572476 36.0 35.0 39.0 31.0 41.0 76-77 34.701021808193595 36.0 35.0 39.0 30.5 40.5 78-79 34.344187624929546 35.0 34.5 37.0 30.0 39.5 80-81 34.01942132660522 35.0 34.0 37.0 30.0 39.0 82-83 33.72875664061846 35.0 34.0 36.5 30.0 39.0 84-85 33.51167170933072 35.0 34.0 36.0 30.0 37.0 86-87 33.30465301265629 35.0 34.0 36.0 30.0 37.0 88-89 33.10289196198089 35.0 34.0 35.5 30.0 37.0 90-91 32.9270046683595 35.0 34.0 35.0 29.5 36.0 92-93 32.75321783930447 35.0 34.0 35.0 29.0 36.0 94-95 32.60107844021998 35.0 34.0 35.0 29.0 36.0 96-97 32.45618286092244 35.0 34.0 35.0 29.0 36.0 98-99 32.32831012302876 35.0 34.0 35.0 29.0 35.5 100 32.22385753803073 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1504702435388765 1101 2 0.051020484101989894 1101 3 0.09191634783340419 1101 4 0.1463767960122837 1101 5 0.1005891470850031 1101 6 0.08788124020117039 1101 7 0.153596963740263 1101 8 0.1408085055821644 1101 9 0.22906371190493502 1101 10-11 0.19784869027353125 1101 12-13 0.18548872954397666 1101 14-15 0.2808066807645062 1101 16-17 0.269193775294454 1101 18-19 0.2088743131675841 1101 20-21 0.28545049969599745 1101 22-23 0.26954154248705464 1101 24-25 0.30634000529462213 1101 26-27 0.28500683485872713 1101 28-29 0.3011088581077601 1101 30-31 0.2688099291940489 1101 32-33 0.33681139976088303 1101 34-35 0.37233091692829134 1101 36-37 0.32687674687304735 1101 38-39 0.2604377955198274 1101 40-41 0.34698840967686095 1101 42-43 0.3616153614154314 1101 44-45 0.3312071208077967 1101 46-47 0.237073272864464 1101 48-49 0.2995469819610932 1101 50-51 0.35149082520615593 1101 52-53 0.3018541824560046 1101 54-55 0.3899722561596519 1101 56-57 0.3308592449788037 1101 58-59 0.3201410405880125 1101 60-61 0.26844485985846234 1101 62-63 0.3116169248117444 1101 64-65 0.30265844513096596 1101 66-67 0.34075181704107393 1101 68-69 0.2534419819048779 1101 70-71 0.2027633610030577 1101 72-73 0.23422915899921293 1101 74-75 0.23989386750390196 1101 76-77 0.21182709232207486 1101 78-79 0.20559042656623916 1101 80-81 0.2673930891916214 1101 82-83 0.2027589776498857 1101 84-85 0.244518777094207 1101 86-87 0.26163068576087056 1101 88-89 0.3184508732645597 1101 90-91 0.3854545453252243 1101 92-93 0.442572297522581 1101 94-95 0.4197844630397327 1101 96-97 0.35192737853697054 1101 98-99 0.3232863197585232 1101 100 0.33822043233302423 1102 1 0.1329862094981067 1102 2 0.09576520031305336 1102 3 0.12782622935230847 1102 4 0.09861000679110532 1102 5 0.1188645507538979 1102 6 0.14048422602689925 1102 7 0.14503310386239576 1102 8 0.1482691108589549 1102 9 0.2244839736067803 1102 10-11 0.22830806296684614 1102 12-13 0.20038684565316345 1102 14-15 0.25993810091007674 1102 16-17 0.273230904122741 1102 18-19 0.2371177189339022 1102 20-21 0.24713791617108427 1102 22-23 0.32105754979634327 1102 24-25 0.32632395795497615 1102 26-27 0.3690145259685522 1102 28-29 0.3042455235515149 1102 30-31 0.3399617129209531 1102 32-33 0.29202247418482585 1102 34-35 0.29480902559447486 1102 36-37 0.3158252854126147 1102 38-39 0.2886477693285272 1102 40-41 0.2869103020664312 1102 42-43 0.24797925771653695 1102 44-45 0.2065910384525651 1102 46-47 0.2943406766178214 1102 48-49 0.2787589027414299 1102 50-51 0.26694712303915225 1102 52-53 0.2811778363578199 1102 54-55 0.2732252208264683 1102 56-57 0.25852128659806795 1102 58-59 0.24661008919368044 1102 60-61 0.23366242712049257 1102 62-63 0.18593365917812577 1102 64-65 0.20409003674728154 1102 66-67 0.30961896635963626 1102 68-69 0.29800150266656544 1102 70-71 0.27093706113775795 1102 72-73 0.2818015149932549 1102 74-75 0.2974485221561807 1102 76-77 0.15782458316568437 1102 78-79 0.2546122338802732 1102 80-81 0.2703078280220552 1102 82-83 0.25598177437390035 1102 84-85 0.31876873552420903 1102 86-87 0.31608716571005857 1102 88-89 0.3640153322783064 1102 90-91 0.3183735423313081 1102 92-93 0.3272005913402225 1102 94-95 0.32677785670894366 1102 96-97 0.42762854786367654 1102 98-99 0.31669824458588636 1102 100 0.3951097470394842 1103 1 0.149360047556776 1103 2 0.1632644569291699 1103 3 0.18462075804692546 1103 4 0.12252111100706031 1103 5 0.1766804242622797 1103 6 0.2006918563776452 1103 7 0.18238329584936253 1103 8 0.18215448752833652 1103 9 0.22253824709448367 1103 10-11 0.25760385218195836 1103 12-13 0.21254602030312952 1103 14-15 0.2680409852395442 1103 16-17 0.308244285032643 1103 18-19 0.27784869153858693 1103 20-21 0.28750366104173963 1103 22-23 0.33567958757316774 1103 24-25 0.32577126819816016 1103 26-27 0.2964958120873149 1103 28-29 0.3263983632779528 1103 30-31 0.3607893900725685 1103 32-33 0.351535202209746 1103 34-35 0.3611253322047787 1103 36-37 0.32395725867664993 1103 38-39 0.3520390219626961 1103 40-41 0.3122952456338908 1103 42-43 0.3997459256760436 1103 44-45 0.4447453778751864 1103 46-47 0.39369554869115575 1103 48-49 0.3909387628559813 1103 50-51 0.3754035995288234 1103 52-53 0.44820973075852777 1103 54-55 0.5102127707624717 1103 56-57 0.45953552078338333 1103 58-59 0.3723712496482392 1103 60-61 0.36744566930849487 1103 62-63 0.3613819519808743 1103 64-65 0.3760444252978772 1103 66-67 0.38499809214018654 1103 68-69 0.39977075630913816 1103 70-71 0.3996071150492426 1103 72-73 0.4024753440020348 1103 74-75 0.4262316997021074 1103 76-77 0.4324435181228239 1103 78-79 0.4275168609601323 1103 80-81 0.4284029553023032 1103 82-83 0.4200918469023094 1103 84-85 0.44086792232688055 1103 86-87 0.3951832109077955 1103 88-89 0.40415482988007767 1103 90-91 0.36818672672929864 1103 92-93 0.47221539437581583 1103 94-95 0.40543110368047053 1103 96-97 0.4620827748090761 1103 98-99 0.4855498134490901 1103 100 0.42713343189642927 1104 1 0.1832348339284664 1104 2 0.07322698377970482 1104 3 0.13182124723615374 1104 4 0.09959820534845676 1104 5 0.10881325795362073 1104 6 0.12310362200582858 1104 7 0.1415391308420766 1104 8 0.13673201304614224 1104 9 0.1564349842650259 1104 10-11 0.17074532508055995 1104 12-13 0.20967281752478328 1104 14-15 0.30568268548570643 1104 16-17 0.27837551945150096 1104 18-19 0.21803276466610555 1104 20-21 0.1866514996720099 1104 22-23 0.23696370930603194 1104 24-25 0.22801573738080805 1104 26-27 0.17386924221322175 1104 28-29 0.2650654794133587 1104 30-31 0.20187736573853954 1104 32-33 0.24605377415348784 1104 34-35 0.28410546673181614 1104 36-37 0.26418836923028266 1104 38-39 0.26237392843661667 1104 40-41 0.26964797929230855 1104 42-43 0.24188832364403368 1104 44-45 0.28792651900314326 1104 46-47 0.28463535646487514 1104 48-49 0.27925623412394884 1104 50-51 0.2264439106030025 1104 52-53 0.2840687125550474 1104 54-55 0.1515327977305958 1104 56-57 0.2066897565036072 1104 58-59 0.17715025214121738 1104 60-61 0.2470768958877727 1104 62-63 0.26881229739237256 1104 64-65 0.2343676978484055 1104 66-67 0.28181757817791464 1104 68-69 0.2835216774587366 1104 70-71 0.28579614278564947 1104 72-73 0.19540875468580055 1104 74-75 0.1791676190486058 1104 76-77 0.18283549667375354 1104 78-79 0.22401727645739555 1104 80-81 0.19095018871769298 1104 82-83 0.18137298959818082 1104 84-85 0.23891273331482665 1104 86-87 0.17582025444877303 1104 88-89 0.1994949888665829 1104 90-91 0.2795015795805469 1104 92-93 0.2331241574482945 1104 94-95 0.2371390326225935 1104 96-97 0.24848974261979606 1104 98-99 0.23968910694695467 1104 100 0.2134659237824721 1105 1 0.09778412844243434 1105 2 0.06550365661536972 1105 3 0.10884463995369487 1105 4 0.050494349298546126 1105 5 0.006268013562248598 1105 6 0.05602670020248013 1105 7 0.0753367261205895 1105 8 0.08304460614385789 1105 9 0.10086958394289525 1105 10-11 0.10954775536107064 1105 12-13 0.12398799342934552 1105 14-15 0.13937303891943742 1105 16-17 0.17661095067728638 1105 18-19 0.18807321415313538 1105 20-21 0.14397348804006072 1105 22-23 0.18996614465695671 1105 24-25 0.2025091616228707 1105 26-27 0.13654142286897297 1105 28-29 0.14280201552376326 1105 30-31 0.14527395334986437 1105 32-33 0.15268662598719374 1105 34-35 0.16883859221266562 1105 36-37 0.14824235672291763 1105 38-39 0.17278740777027224 1105 40-41 0.16124957564491638 1105 42-43 0.2221251559597448 1105 44-45 0.21126263416569913 1105 46-47 0.228132058884853 1105 48-49 0.2714984359046966 1105 50-51 0.31136202858946405 1105 52-53 0.2768848929707204 1105 54-55 0.3154230677611878 1105 56-57 0.28914451987307643 1105 58-59 0.24881059544964756 1105 60-61 0.355795175403145 1105 62-63 0.3071545229212873 1105 64-65 0.38959616139393205 1105 66-67 0.3275767717958118 1105 68-69 0.2947441077289028 1105 70-71 0.3579440607339279 1105 72-73 0.27561216365970864 1105 74-75 0.2676036601727674 1105 76-77 0.22257577247324178 1105 78-79 0.19641126470541082 1105 80-81 0.2985408184889877 1105 82-83 0.2961248031766459 1105 84-85 0.22215753704582397 1105 86-87 0.2218816680284803 1105 88-89 0.24834416008670246 1105 90-91 0.25350717048815596 1105 92-93 0.28828243714993107 1105 94-95 0.17239016816252217 1105 96-97 0.21599349757185848 1105 98-99 0.20110066883626843 1105 100 0.17667140334792464 1106 1 -0.002498891754321164 1106 2 0.0077506337423471905 1106 3 0.013994138606506112 1106 4 -0.01824243286343119 1106 5 0.02253941111658264 1106 6 0.002864580841006159 1106 7 0.06839251923111078 1106 8 0.033932603391129135 1106 9 -0.014541886977355034 1106 10-11 0.07447634130645042 1106 12-13 0.08590577973451019 1106 14-15 0.09539675104171863 1106 16-17 0.08943559888911068 1106 18-19 0.11978239253937062 1106 20-21 0.06187549644715773 1106 22-23 0.043080875994021994 1106 24-25 0.061246819105043926 1106 26-27 0.042124808687979964 1106 28-29 0.01578374849676578 1106 30-31 0.0274847807588543 1106 32-33 0.07850349089208208 1106 34-35 0.07582450257585549 1106 36-37 0.07289942236791092 1106 38-39 0.07804456074606492 1106 40-41 0.12763149495046378 1106 42-43 0.14427548566368387 1106 44-45 0.1060921580116343 1106 46-47 0.06699944485679055 1106 48-49 0.06825411693399985 1106 50-51 0.039169140213921594 1106 52-53 0.15339431725150376 1106 54-55 0.16951222163845614 1106 56-57 0.09289421049265911 1106 58-59 0.10733571110258566 1106 60-61 0.04317129747308712 1106 62-63 0.03960359840205996 1106 64-65 -0.038472892314501905 1106 66-67 -0.03033637430619507 1106 68-69 -0.010815332549107382 1106 70-71 0.014459612256082721 1106 72-73 0.02207919024730387 1106 74-75 0.11250597645111071 1106 76-77 0.10480546454163431 1106 78-79 0.054457218009595465 1106 80-81 -0.029770910197974843 1106 82-83 0.022719797552461785 1106 84-85 0.042714381546694824 1106 86-87 -0.0022274672960165276 1106 88-89 0.051110876187159704 1106 90-91 0.013142986980803073 1106 92-93 0.04780909014684909 1106 94-95 -0.020728356228723044 1106 96-97 -7.473533198592008E-4 1106 98-99 0.07253902114157995 1106 100 0.02704547696738757 1107 1 0.039472999112653895 1107 2 -0.001887202167161206 1107 3 0.026624922798539785 1107 4 0.02567368932724179 1107 5 0.04639918798324061 1107 6 0.03866658542486334 1107 7 0.037880280127552624 1107 8 -0.007937152413219906 1107 9 0.012767513846569045 1107 10-11 0.014333572126801641 1107 12-13 0.08453318711657687 1107 14-15 0.08322936193644637 1107 16-17 0.0980548941083157 1107 18-19 0.09833146379445878 1107 20-21 0.11590421154897967 1107 22-23 0.11149719610192932 1107 24-25 0.06402279146504952 1107 26-27 0.11560475551910088 1107 28-29 0.10639086569797485 1107 30-31 0.10848362746341422 1107 32-33 0.12827588608147522 1107 34-35 0.03230959036802261 1107 36-37 0.06705768773336729 1107 38-39 0.05501224892624634 1107 40-41 0.05742194703250192 1107 42-43 0.1387832826250417 1107 44-45 0.12542537243173513 1107 46-47 0.173264258106002 1107 48-49 0.08025535330580169 1107 50-51 0.1605883044656693 1107 52-53 0.2115538825177623 1107 54-55 0.20630234630186806 1107 56-57 0.1494130175160251 1107 58-59 0.1768491279358102 1107 60-61 0.18449470399507106 1107 62-63 0.22044105704713957 1107 64-65 0.18105445202726145 1107 66-67 0.15022485541950914 1107 68-69 0.10766683779654329 1107 70-71 0.171066626833543 1107 72-73 0.15359433213685492 1107 74-75 0.14319292026704744 1107 76-77 0.20290985732669498 1107 78-79 0.11888000830041534 1107 80-81 0.09891487054883896 1107 82-83 0.12901576443920248 1107 84-85 0.16329813962831707 1107 86-87 0.203071461018844 1107 88-89 0.15180798464091794 1107 90-91 0.1707135652411793 1107 92-93 0.22121439041904267 1107 94-95 0.2638074667892951 1107 96-97 0.22251635061552122 1107 98-99 0.268255823581093 1107 100 0.2798175398513081 1108 1 -0.02943711885511391 1108 2 -0.07302589348324062 1108 3 -0.09055479327253124 1108 4 -0.089981756483418 1108 5 -0.006025918322137613 1108 6 0.005257662492660131 1108 7 0.02879872974888542 1108 8 0.01850096970117221 1108 9 0.027921668634867558 1108 10-11 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-0.1216894604659089 2214 74-75 -0.15987578836652716 2214 76-77 -0.19776261879905377 2214 78-79 -0.058273078247580656 2214 80-81 -0.10065021037185318 2214 82-83 -0.09380563195363578 2214 84-85 0.04043959672014097 2214 86-87 0.03785776129366525 2214 88-89 0.05370618286556095 2214 90-91 -0.022677403526394357 2214 92-93 0.03525295053429289 2214 94-95 -0.06397801377822532 2214 96-97 0.013014407480170576 2214 98-99 -0.04340592408906474 2214 100 -0.05560984830832183 2215 1 -0.19888710310582525 2215 2 -0.15329072522685294 2215 3 -0.227098545850275 2215 4 -0.10590420383640264 2215 5 -0.1626582845967235 2215 6 -0.23560579027425632 2215 7 -0.2141760853056951 2215 8 -0.19271487565296042 2215 9 -0.2853237035966103 2215 10-11 -0.2213308589692673 2215 12-13 -0.26107872604614357 2215 14-15 -0.3102326395999171 2215 16-17 -0.3086914372301024 2215 18-19 -0.29113127290492 2215 20-21 -0.3507272010425595 2215 22-23 -0.41002470141106784 2215 24-25 -0.3725348406937101 2215 26-27 -0.3701083195973922 2215 28-29 -0.43967491158284844 2215 30-31 -0.48129705623819774 2215 32-33 -0.43014943434175024 2215 34-35 -0.4354896088765656 2215 36-37 -0.45127153323594626 2215 38-39 -0.47723477465399355 2215 40-41 -0.4561658025982638 2215 42-43 -0.3786146584782699 2215 44-45 -0.4428882208206133 2215 46-47 -0.3940498215865489 2215 48-49 -0.41138590914960105 2215 50-51 -0.3899822172579519 2215 52-53 -0.28751890962700344 2215 54-55 -0.3203360877568642 2215 56-57 -0.322287608252978 2215 58-59 -0.3672361113553464 2215 60-61 -0.4060842038696322 2215 62-63 -0.32457656265347623 2215 64-65 -0.4202260302748968 2215 66-67 -0.45143960649178183 2215 68-69 -0.34362641080961964 2215 70-71 -0.3897288239600556 2215 72-73 -0.4543050385724001 2215 74-75 -0.4361628656200267 2215 76-77 -0.4379628956387194 2215 78-79 -0.43131996044414933 2215 80-81 -0.4276783311741781 2215 82-83 -0.4225704488329072 2215 84-85 -0.4143700729350499 2215 86-87 -0.3763011631829798 2215 88-89 -0.3786424017598904 2215 90-91 -0.38691573210585517 2215 92-93 -0.447112849572072 2215 94-95 -0.46564670768205474 2215 96-97 -0.48436171577366594 2215 98-99 -0.44900818138455634 2215 100 -0.38365278179351847 2216 1 -0.21892744248188478 2216 2 -0.20156407888941885 2216 3 -0.22121041305548772 2216 4 -0.19458991468638231 2216 5 -0.2479447364918812 2216 6 -0.24814252311706753 2216 7 -0.2108286449257335 2216 8 -0.2835870534689917 2216 9 -0.26401539138296926 2216 10-11 -0.304229107461893 2216 12-13 -0.3468892971145223 2216 14-15 -0.4645555745406966 2216 16-17 -0.4814754316985699 2216 18-19 -0.42161370779250973 2216 20-21 -0.47344515649520247 2216 22-23 -0.4126981047561742 2216 24-25 -0.44677642047196997 2216 26-27 -0.4619886851438082 2216 28-29 -0.47300582539747893 2216 30-31 -0.467077801958915 2216 32-33 -0.4493334816572485 2216 34-35 -0.46201775249392085 2216 36-37 -0.40410426880971784 2216 38-39 -0.4355596858207349 2216 40-41 -0.3913186139932918 2216 42-43 -0.42334813102293367 2216 44-45 -0.4335946978655727 2216 46-47 -0.32447824253216595 2216 48-49 -0.4039038607257339 2216 50-51 -0.5013796930209011 2216 52-53 -0.49862788250768375 2216 54-55 -0.515940033246892 2216 56-57 -0.543777503037532 2216 58-59 -0.534941489925643 2216 60-61 -0.5871150651932382 2216 62-63 -0.5370818681239768 2216 64-65 -0.566392088633684 2216 66-67 -0.6572919607362238 2216 68-69 -0.5885132821583454 2216 70-71 -0.5535489961005737 2216 72-73 -0.5136062552013385 2216 74-75 -0.6026289495716526 2216 76-77 -0.5321286185612379 2216 78-79 -0.6802094211393666 2216 80-81 -0.5523959496082966 2216 82-83 -0.5344752323993802 2216 84-85 -0.7262485714305242 2216 86-87 -0.7059060643499251 2216 88-89 -0.7984466303929381 2216 90-91 -0.7552278231198386 2216 92-93 -0.7078919042667735 2216 94-95 -0.5719999814095331 2216 96-97 -0.7250523703530263 2216 98-99 -0.8420078677041758 2216 100 -0.8979198990312653 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12.0 3 4.0 4 8.0 5 20.0 6 49.0 7 103.0 8 208.0 9 421.0 10 782.0 11 1178.0 12 1934.0 13 2949.0 14 3591.0 15 4913.0 16 5875.0 17 8480.0 18 9997.0 19 5276.0 20 3548.0 21 3458.0 22 3650.0 23 3799.0 24 4483.0 25 5387.0 26 6875.0 27 8902.0 28 11568.0 29 14966.0 30 19271.0 31 24809.0 32 31378.0 33 41032.0 34 57445.0 35 88215.0 36 162033.0 37 362314.0 38 644889.0 39 305596.0 40 1125.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.17432841243035 19.605513256596943 13.64858611436072 23.571572216611994 2 15.851637011331613 19.915643997300087 39.97608865939395 24.25663033197435 3 18.707602652041423 24.90609289710505 29.708203030642004 26.67810142021152 4 12.387131429300192 16.684148494482283 38.443052743241935 32.485667332975595 5 12.864171712504763 37.45424971697374 35.59927264277628 14.082305927745221 6 31.269034984382937 37.85462621721227 17.36385055173085 13.512488246673943 7 27.582660873051857 32.2051959884207 22.058768696539342 18.153374441988106 8 25.54265358793344 35.885278643593 19.884292983222156 18.687774785251406 9 25.039245237749135 16.452576351914004 21.064898248784274 37.443280161552586 10-11 24.266055454878774 26.48395879778323 28.407460235073007 20.842525512264988 12-13 25.207925457554893 23.684237545412344 28.805383068645256 22.302453928387507 14-15 22.13714181343037 25.558608122939447 26.35510665282564 25.94914341080454 16-17 21.389729484366725 29.152382547796858 26.81852324186454 22.639364725971877 18-19 21.1942991559729 27.623068596993406 29.601228402843937 21.581403844189754 20-21 22.379605542592387 26.932055581553943 29.353589380840855 21.33474949501281 22-23 21.88344432342875 26.73348678399635 29.409029416000354 21.974039476574546 24-25 21.761032663042993 26.8823055617175 29.398765155566025 21.95789661967348 26-27 21.556918100414446 26.846642913246153 29.44542418823041 22.151014798108985 28-29 21.561629474719474 26.91340612309413 29.494119268211627 22.03084513397477 30-31 21.815124724438466 27.383257693229464 29.125884640668303 21.67573294166377 32-33 21.453784105530108 26.956817539500566 29.665211778380723 21.9241865765886 34-35 21.57029044988417 26.802619555449404 29.63838181549956 21.98870817916687 36-37 21.9733343132259 27.143114210261526 28.967038323346173 21.916513153166395 38-39 21.894762780437958 27.343271677556263 29.058606041578066 21.703359500427712 40-41 21.719879905454725 27.097871866728774 29.20282274058087 21.97942548723563 42-43 21.736457893710117 27.046547959166578 29.055039520832533 22.16195462629077 44-45 21.834591252405374 27.272265491804298 28.872255332623993 22.020887923166335 46-47 22.028426251105756 27.484851743515282 28.467103979750807 22.019618025628155 48-49 22.04598866386785 27.449834994377326 28.242142981816688 22.262033359938137 50-51 22.089354313841937 27.483825017846115 28.134228710167775 22.29259195814418 52-53 22.09162391795273 27.395904877649425 28.142712706486687 22.369758497911153 54-55 22.18230000599824 27.852446552174147 27.925884456616245 22.039368985211368 56-57 21.908596557875175 27.51884176698407 28.120232818151216 22.452328856989542 58-59 21.812651746001038 27.143519496709885 28.503741874682188 22.54008688260689 60-61 21.986168384090508 27.535242358594207 28.180134155218223 22.29845510209706 62-63 21.93653430371518 27.53575572142879 28.118962920613033 22.408747054242998 64-65 21.94120860741955 27.25540557555269 28.268486600959825 22.534899216067934 66-67 21.781450574628792 27.335983907421742 28.181735896365524 22.70082962158394 68-69 22.191129179120246 27.45772988302327 28.05098946847378 22.300151469382705 70-71 22.17432032732041 27.316355780186502 28.050086961032754 22.45923693146033 72-73 22.072160675522834 27.44365554430957 28.09030567883809 22.393878101329506 74-75 22.096907415378055 27.485227956495926 27.989459290115125 22.428405338010887 76-77 22.0823353151846 27.57331971570499 27.793822622285084 22.55052234682532 78-79 22.24276874409295 27.576041194395373 27.88081660355906 22.300373457952613 80-81 22.15238986610957 27.377721025666517 28.001483888782914 22.468405219440996 82-83 21.977822155202766 27.489049820849637 28.119081965512237 22.41404605843536 84-85 22.000623600748536 27.419168319785058 27.943284754799 22.636923324667407 86-87 22.072088380975437 27.524244353251547 27.898703833596137 22.504963432176876 88-89 21.801829016218832 27.42602957141923 27.947995761152512 22.82414565120943 90-91 21.740185376524447 27.231662293083396 28.0945623462581 22.933589984134052 92-93 21.893661873267728 27.47619467061076 27.91142110021394 22.718722355907577 94-95 21.704998027592097 27.237791335455245 28.017892171431967 23.03931846552069 96-97 21.659301689374033 27.241328532926246 28.065953810717158 23.033415966982567 98-99 21.627789286427397 27.31015614086803 27.943403562364615 23.118651010339953 100 21.767935141199096 27.16024431747871 27.859768727341113 23.212051813981084 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9.0 1 8.0 2 39.5 3 75.0 4 51.5 5 44.5 6 125.5 7 279.0 8 493.0 9 623.5 10 539.0 11 445.5 12 509.5 13 661.0 14 781.5 15 872.0 16 1028.5 17 1197.0 18 1408.5 19 1683.5 20 2064.0 21 2530.5 22 3237.0 23 4412.5 24 5991.0 25 7828.5 26 10307.5 27 13545.5 28 17606.0 29 22344.0 30 26864.5 31 31745.0 32 37252.0 33 42653.5 34 47779.0 35 51954.5 36 55546.0 37 59849.0 38 62949.0 39 64056.0 40 65432.5 41 66484.0 42 66590.5 43 66444.5 44 65619.0 45 64868.0 46 63780.5 47 61800.5 48 60661.0 49 59619.0 50 58051.0 51 56442.0 52 53279.5 53 50929.0 54 49202.0 55 46446.0 56 42905.5 57 39505.5 58 36193.5 59 32101.0 60 28585.0 61 24979.5 62 21543.0 63 17998.5 64 14506.5 65 12174.5 66 10014.0 67 7760.5 68 6045.0 69 4787.0 70 3693.0 71 2746.5 72 2179.5 73 1723.5 74 1310.5 75 898.5 76 578.0 77 424.5 78 330.0 79 224.5 80 150.5 81 84.0 82 30.5 83 14.0 84 9.5 85 6.0 86 3.0 87 1.0 88 0.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.040366530256254515 2 0.24625204602108677 3 0.002377680497021685 4 0.0027019096557064602 5 4.323055449130336E-4 6 1.621145793423876E-4 7 0.0 8 3.782673517989044E-4 9 0.0 10-11 6.214392208124858E-4 12-13 0.0 14-15 3.242291586847752E-4 16-17 1.621145793423876E-4 18-19 5.40381931141292E-4 20-21 5.40381931141292E-5 22-23 1.35095482785323E-4 24-25 0.0014320121175244239 26-27 1.35095482785323E-4 28-29 4.323055449130336E-4 30-31 1.891336758994522E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.40381931141292E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.70190965570646E-5 68-69 2.161527724565168E-4 70-71 8.10572896711938E-5 72-73 3.242291586847752E-4 74-75 1.891336758994522E-4 76-77 2.70190965570646E-5 78-79 0.0 80-81 0.0 82-83 1.35095482785323E-4 84-85 0.0 86-87 4.8634373802716285E-4 88-89 0.002161527724565168 90-91 0.002107489531451039 92-93 6.484583173695504E-4 94-95 7.024965104836797E-4 96-97 2.9721006212771065E-4 98-99 1.35095482785323E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1850543.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.29617111177872 #Duplication Level Percentage of deduplicated Percentage of total 1 78.67272018273869 52.943728392466696 2 13.438639061641771 18.08737907603356 3 3.8887156368644815 7.850870187104449 4 1.5925546754641542 4.286913277795958 5 0.7594376697339867 2.5553623685574434 6 0.42627749442663626 1.7212105923621126 7 0.26862448801180117 1.2654179657039284 8 0.18757006460891934 1.009819773069538 9 0.1342559525006946 0.813142039703542 >10 0.5954004013406105 6.877236796812797 >50 0.026637901474326396 1.2004556885071402 >100 0.00884502919033792 0.9814071428689116 >500 8.036050089783898E-5 0.045534972840394414 >1k 2.4108150269351692E-4 0.36152172617344025 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2932 0.15843998221062683 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2521 0.13623028484071972 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 5.403819311412921E-5 0.0 0.0 0.0 6 0.0 5.403819311412921E-5 0.0 0.0 0.0 7 0.0 5.403819311412921E-5 0.0 0.0 0.0 8 0.0 5.403819311412921E-5 0.0 0.0 0.0 9 0.0 5.403819311412921E-5 0.0 0.0 0.0 10-11 0.0 5.403819311412921E-5 0.0 0.0 0.0 12-13 0.0 5.403819311412921E-5 0.0 0.0 0.0 14-15 0.0 5.403819311412921E-5 0.0 0.0 0.0 16-17 0.0 5.403819311412921E-5 0.0 0.0 0.0 18-19 0.0 5.403819311412921E-5 0.0 0.0 0.0 20-21 0.0 5.403819311412921E-5 0.0 0.0 0.0 22-23 0.0 5.403819311412921E-5 0.0 8.105728967119382E-5 0.0 24-25 0.0 8.105728967119382E-5 0.0 1.621145793423876E-4 0.0 26-27 0.0 1.0807638622825841E-4 0.0 2.9721006212771065E-4 0.0 28-29 0.0 1.0807638622825841E-4 0.0 7.295156070407443E-4 0.0 30-31 0.0 1.0807638622825841E-4 0.0 0.002782966945377654 0.0 32-33 0.0 1.0807638622825841E-4 0.0 0.008213805353347639 0.0 34-35 0.0 1.0807638622825841E-4 0.0 0.01567107600309747 0.0 36-37 0.0 1.0807638622825841E-4 0.0 0.029396777054086286 0.0 38-39 0.0 1.0807638622825841E-4 0.0 0.0541192504038004 0.0 40-41 0.0 1.0807638622825841E-4 0.0 0.08389429480968559 0.0 42-43 0.0 1.0807638622825841E-4 0.0 0.11375039650524196 0.0 44-45 0.0 1.0807638622825841E-4 0.0 0.14720003804288795 0.0 46-47 0.0 1.0807638622825841E-4 0.0 0.18826906480962613 0.0 48-49 0.0 1.0807638622825841E-4 0.0 0.2387677562747799 0.0 50-51 0.0 1.0807638622825841E-4 0.0 0.2951025725962596 0.0 52-53 0.0 1.0807638622825841E-4 0.0 0.35092402608315504 0.0 54-55 0.0 1.0807638622825841E-4 0.0 0.41039305760525424 0.0 56-57 0.0 1.0807638622825841E-4 0.0 0.48445240126816835 0.0 58-59 0.0 1.0807638622825841E-4 0.0 0.5677252568570414 0.0 60-61 0.0 1.0807638622825841E-4 0.0 0.657050390074697 0.0 62-63 0.0 1.0807638622825841E-4 0.0 0.7496178148792003 0.0 64-65 0.0 1.0807638622825841E-4 0.0 0.8512096179337632 0.0 66-67 0.0 1.35095482785323E-4 0.0 0.9646357852803205 0.0 68-69 0.0 1.891336758994522E-4 0.0 1.0940032195955456 0.0 70-71 0.0 2.1615277245651683E-4 0.0 1.2357994383270208 0.0 72-73 0.0 2.1615277245651683E-4 0.0 1.3891328112883623 0.0 74-75 0.0 2.1615277245651683E-4 0.0 1.5463569341539214 0.0 76-77 0.0 2.1615277245651683E-4 0.0 1.7170365671048984 0.0 78-79 0.0 2.1615277245651683E-4 0.0 1.9058460138456659 0.0 80-81 0.0 2.1615277245651683E-4 0.0 2.1219987863021825 0.0 82-83 0.0 2.1615277245651683E-4 0.0 2.3470138224294166 5.403819311412921E-5 84-85 0.0 2.1615277245651683E-4 0.0 2.5737580807363027 5.403819311412921E-5 86-87 0.0 2.1615277245651683E-4 0.0 2.8073922086652408 5.403819311412921E-5 88 0.0 2.1615277245651683E-4 0.0 3.0061446829390075 5.403819311412921E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2445 0.0 50.507504 1 GTATCAA 4505 0.0 44.989082 1 TCAACGC 5275 0.0 37.95449 4 ATCAACG 5335 0.0 37.44055 3 CAACGCA 5435 0.0 36.838146 5 TATCAAC 5650 0.0 35.60419 2 AACGCAG 5750 0.0 35.06527 6 ACGCAGA 6625 0.0 30.221182 7 CGCAGAG 6945 0.0 28.828703 8 GTGGTAT 1025 0.0 28.050425 1 GCAGAGT 7580 0.0 26.227623 9 GTACATG 5145 0.0 25.742718 1 TACACCG 300 0.0 25.066082 5 TACATGG 5215 0.0 23.758724 2 ACATGGG 5130 0.0 23.087181 3 GAGTACT 5145 0.0 21.831453 12-13 AGAGTAC 7045 0.0 20.747564 10-11 CAGAGTA 7620 0.0 20.415537 10-11 TGGTATC 1295 0.0 20.372293 2 GGACCGT 190 9.047888E-6 19.789013 6 >>END_MODULE